Article
Infectious Diseases
Yin-Chih Feng, Ci-Hong Liou, Wailap Victor Ng, Feng-Jui Chen, Chih-Hsin Hung, Po-Yen Liu, Yu-Chieh Liao, Han-Chieh Wu, Ming-Fang Cheng
Summary: Third-generation cephalosporin-resistant Escherichia coli (CREC), particularly strains producing extended-spectrum beta-lactamases (ESBLs), are a global concern. A longitudinal study was conducted to investigate the distribution of antibiotic resistance genes among CREC isolates from fecal samples, family members, and home environment. The study revealed a wide distribution of ESBL genes, particularly bla(CTX-M) and bla(CMY-2), among CREC isolates, indicating plasmid-mediated household transmission.
Article
Microbiology
Masato Kawamura, Ryota Ito, Yurina Tamura, Mio Takahashi, Miho Umenai, Yuriko Chiba, Takumi Sato, Shigeru Fujimura
Summary: This study is the first to demonstrate that mutations in the chromosomal-ampC attenuator region are responsible for the emergence of cefazolin resistance in Escherichia coli strains. The resistance was maintained even after culturing E. coli without cefazolin. This study highlights one of the key factors contributing to the increase in the number of cefazolin-resistant E. coli strains, which can pose a considerable challenge for treating common infections, such as urinary tract infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Cecilia G. G. Carvalhaes, Dee Shortridge, Leah N. N. Woosley, Nabina Gurung, Mariana Castanheira
Summary: This study evaluated the performance of the Vitek 2 Advanced Expert System (AES) confidence level report as a rapid tool for reporting antimicrobial susceptibility testing (AST) results for Enterobacterales isolates. The results showed that the AES assessment was accurate and enabled rapid adjustments to antimicrobial therapy.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Faheema Ebrahim Choonara, Bjorg Christina Haldorsen, Jessin Janice, Joshua Mbanga, Isaac Ndhlovu, Osborne Saulosi, Tarsizio Maida, Fanuel Lampiao, Gunnar Skov Simonsen, Sabiha Yusuf Essack, Arnfinn Sundsfjord
Summary: The global rise in infections caused by multidrug resistant Enterobacterales is a public health problem. This study characterized the molecular epidemiology of MDR E. coli and K. pneumoniae isolates from a tertiary hospital in Malawi, identifying dominant sequence types and resistance determinants. The findings provide valuable insights into the transmission and resistance patterns of these bacteria in the region.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Article
Microbiology
Junxin Zhou, Weiping Wang, Min Liang, Qian Yu, Shiqi Cai, Tailong Lei, Yan Jiang, Xiaoxing Du, Zhihui Zhou, Yunsong Yu
Summary: The study aimed to investigate the molecular mechanism underlying ceftazidime-avibactam resistance caused by a novel CMY-178 variant. The results showed that CMY-178 increased the resistance to multiple cephalosporins and ceftazidime-avibactam compared with CMY-172. Structural modeling and molecular docking revealed that CMY-178 had a narrower ligand-binding pocket and a stronger positive charge at the pocket entrance than CMY-172. The bla(CMY-178) gene was located in a transferable plasmid with high transfer frequency and no fitness costs.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Mohammad M. Obaidat, Wasan A. Gharaibeh
Summary: Limited data is available on the prevalence and antimicrobial resistance of ESBL and AmpC beta-lactamases E. coli in sheep and goats in Jordan. This study found a high prevalence of ESBL and AmpC beta-lactamases E. coli in milk samples from sheep and goats, with a significant resistance to multiple antimicrobial drugs. Therefore, good hygiene practices and judicious antimicrobial use are crucial in small ruminant farming.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Immunology
Tam Tran, Sylvia Checkley, Niamh Caffrey, Chunu Mainali, Sheryl Gow, Agnes Agunos, Karen Liljebjelke
Summary: This study identified potential horizontal gene transfer events of antimicrobial resistance genes between E. coli and Salmonella in broiler chicken production, revealing various resistant genes in the process.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Min Xu, Jun Zhao, Li Xu, Qing Yang, Hao Xu, Haishen Kong, Jianying Zhou, Yiqi Fu
Summary: This study identified conjugative plasmid-mediated bla(CMY-172) as a new mechanism for CAZ/AVI resistance in clinical KPC-Kp strains. Careful monitoring of CAZ/AVI susceptibility is crucial to prevent the spread of the resistance gene.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Antonella Santona, Jose Joao Sumbana, Maura Fiamma, Massimo Deligios, Elisa Taviani, Samuel Elija Simbine, Tomas Zimba, Jahit Sacarlal, Salvatore Rubino, Bianca Paglietti
Summary: ESBL-producing ExPEC, including high-risk lineages, were identified in extraintestinal infections at Maputo Central Hospital. The majority of isolates showed multidrug resistance, with ESBL genes associated with resistance to cefotaxime and ceftazidime. Furthermore, different sequence types were found to carry CTX-M transposition units, potentially facilitating the dissemination of ESBL genes. Genomic analysis revealed a diverse array of virulence-associated genes and clonality among isolates, suggesting the presence of hospital-acquired infections and long-term persistence.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Microbiology
Christa Ewers, Anno de Jong, Ellen Prenger-Berninghoff, Farid El Garch, Ursula Leidner, Sumeet K. Tiwari, Torsten Semmler
Summary: Livestock animals may serve as a significant source of ESBL/pAmpC E. coli contamination for humans. Analysis of Escherichia spp. isolates from healthy livestock fecal samples revealed a variety of ESBL/pAmpC types, with the majority of genes located on plasmids, indicating the potential dissemination of cephalosporin resistance genes through globally successful plasmid lineages in livestock.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Zoi Athanasakopoulou, Katerina Tsilipounidaki, Marina Sofia, Dimitris C. Chatzopoulos, Alexios Giannakopoulos, Ioannis Karakousis, Vassilios Giannakis, Vassiliki Spyrou, Antonia Touloudi, Maria Satra, Dimitrios Galamatis, Vassilis Diamantopoulos, Spyridoula Mpellou, Efthymia Petinaki, Charalambos Billinis
Summary: This study identified 13 Escherichia coli strains producing plasmid-encoded AmpC beta-lactamases (pAmpC) in fecal samples from animals in Greece, with bla(CMY-2) being the predominant gene. The strains belonged to different sequence types and displayed co-resistance to various antibiotics, including tetracyclines and sulfonamides, along with mutations in quinolone resistance-determining regions. This report provides evidence of pAmpC dissemination and expands the knowledge on antibiotic resistance in animals in Greece.
Article
Microbiology
Anna R. Holtmann, Diana Meemken, Anja Mueller, Diana Seinige, Kathrin Buettner, Klaus Failing, Corinna Kehrenberg
Summary: This study revealed the presence of ESBL-/AmpC-E. coli in wild boars, with a significant positive association with human population density. The E. coli isolates carried various β-lactamase genes and showed multiple drug resistances.
Article
Infectious Diseases
Lurdes Clemente, Celia Leao, Laura Moura, Teresa Albuquerque, Ana Amaro
Summary: This study aimed to characterize ESBL/PMA beta among Escherichia coli producers isolated from meat in Portugal. The results revealed a high frequency of resistance mechanisms, indicating a significant threat to food safety and public health.
Article
Veterinary Sciences
Vangelis Economou, Georgios Delis, Dimitra Stavrou, Panagiota Gousia, Anestis Tsitsos, Tilemachos Mantzios, Eirini Chouliara, Nikolaos Kolovos, Nikolaos Soultos
Summary: Antimicrobial resistance (AMR) is a significant concern in both human and veterinary medicine, particularly among gram-negative bacteria like Escherichia coli. This study found that 64% of raw poultry skin samples collected from a university club in Greece were positive for extended-spectrum β-lactamase (ESBL)-producing E. coli. The isolates were further characterized, and the majority of them were classified as true ESBL or AmpC phenotypes.
VETERINARY SCIENCES
(2023)
Article
Infectious Diseases
Joao Pedro Rueda Furlan, Ralf Lopes, Eliana Guedes Stehling
Summary: This study investigated a multidrug-resistant STEC isolate recovered from a healthy sheep, carrying mobile bla(CMY-97) encoding a pAmpC belonging to the CMY-2-like group. The isolate exhibited multidrug resistance to clinically relevant antimicrobials and virulence genes were detected, highlighting the potential for zoonotic transmission from ovine reservoirs of clinically relevant MDR bacteria.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Microbiology
Elisenda Miro, John W. A. Rossen, Monika A. Chlebowicz, Dag Harmsen, Sylvain Brisse, Virginie Passet, Ferran Navarro, Alex W. Friedrich, S. Garcia-Cobos
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Infectious Diseases
Catherine Ludden, Felix Lotsch, Erik Alm, Narender Kumar, Karin Johansson, Barbara Albiger, Te-Din Huang, Olivier Denis, Anette M. Hammerum, Henrik Hasman, Jari Jalava, Kati Raisanen, Laurent Dortet, Agnes B. Jousset, Soren Gatermann, Sebastian Haller, Martin Cormican, Wendy Brennan, Maria Del Grosso, Monica Monaco, Leo Schouls, Orjan Samuelsen, Mateja Pirs, Tjasa Cerar, Jesus Oteo-Iglesias, Maria Perez-Vazquez, Karin Sjostrom, Petra Edquist, Katie L. Hopkins, Marc J. Struelens, Daniel Palm, Dominique L. Monnet, Anke Kohlenberg
Article
Infectious Diseases
Zaira Moure, Noelia Lara, Mercedes Marin, Pedro J. Sola-Campoy, Veronica Bautista, Frederic Gomez-Bertomeu, Cristina Gomez-Dominguez, Maria Perez-Vazquez, Belen Aracil, Jose Campos, Emilia Cercenado, Jesus Oteo-Iglesias
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2020)
Letter
Infectious Diseases
Jesus Oteo-Iglesias, Maria Perez-Vazquez, Pedro Sola Campoy, Zaira Moure, Isabel Sanchez Romero, Rosario Sanchez Benito, Esteban Aznar, Cristina Seral, Jose Ramon Pano-Pardo, Alicia Avila, Noelia Lara, Veronica Bautista, Belen Aracil, Jose Campos
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Multidisciplinary Sciences
Eva Gato, Juan Carlos Vazquez-Ucha, Soraya Rumbo-Feal, Laura Alvarez-Fraga, Juan A. Vallejo, Marta Martinez-Guitian, Alejandro Beceiro, Jose Ramos Vivas, Pedro J. Sola Campoy, Maria Perez-Vazquez, Jesus Oteo Iglesias, Bruno Kotska Rodino-Janeiro, Antonio Romero, Margarita Poza, German Bou, Astrid Perez
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Environmental Sciences
Roosmarijn E. C. Luiken, Liese Van Gompel, Alex Bossers, Patrick Munk, Philip Joosten, Rasmus Borup Hansen, Berith E. Knudsen, Silvia Garcia-Cobos, Jeroen Dewulf, Frank M. Aarestrup, Jaap A. Wagenaar, Lidwien A. M. Smit, Dik J. Mevius, Dick J. J. Heederik, Heike Schmitt
ENVIRONMENT INTERNATIONAL
(2020)
Article
Environmental Sciences
Liese Van Gompel, Roosmarijn E. C. Luiken, Rasmus B. Hansen, Patrick Munk, Martijn Bouwknegt, Lourens Heres, Gerdit D. Greve, Peter Scherpenisse, Betty G. M. Jongerius-Gortemaker, Monique H. G. Tersteeg-Zijderveld, Silvia Garcia-Cobos, Wietske Dohmen, Alejandro Dorado-Garcia, Jaap A. Wagenaar, Bert A. P. Urlings, Frank M. Aarestrup, Dik J. Mevius, Dick J. J. Heederik, Heike Schmitt, Alex Bossers, Lidwien A. M. Smit
ENVIRONMENT INTERNATIONAL
(2020)
Article
Infectious Diseases
Christina Louka, Sofanne J. Ravensbergen, Alewijn Ott, Xuewei Zhou, Silvia Garcia-Cobos, Alexander W. Friedrich, Spyros Pournaras, Sigrid Rosema, John W. Rossen, Ymkje Stienstra, Erik Bathoorn
Summary: The study found that ESBL-E. coli strains isolated from asylum seekers, mainly from Syria and Iraq, were predominantly classified as ST131 and ST10, with the main resistance genes being bla(CTX_M-15) and bla(TEM-1B). No mcr or carbapenemase genes were detected, and minor clustering was observed among the asylum seeker population.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Julia Guzman-Puche, Rim Jenayeh, Maria Perez-Vazquez, Manuel-Causse, Ferjani Asma, Boukadida Jalel, Jesus Oteo-Iglesias, Luis Martinez-Martinez
Summary: This study aimed to characterize OXA-48-producing Klebsiella oxytoca isolates causing an outbreak in a Tunisian hospital. The isolates were all positive for OXA-48 and negative for ESBL, carrying the bla(OXA-48) gene on a ca.63 kb IncL plasmid and belonging to the new ST220. These findings suggest challenges in detecting OXA-48 and a risk of overlooked production in K. oxytoca, potentially contributing to its hidden spread.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Microbiology
Ilse Verburg, H. Pieter J. van Veelen, Karola Waar, John W. A. Rossen, Alex W. Friedrich, Lucia Hernandez Leal, Silvia Garcia-Cobos, Heike Schmitt
Summary: The study found that the overall bacterial composition differed between clinical wastewater and non-clinical wastewater, but target taxa were not enriched in clinical wastewater. This suggests that treating clinical wastewater before release into the wastewater system would only remove a minor part of the total potential pathogen load. Most target taxa showed a decreased relative abundance after wastewater treatment, yet all investigated taxa were detected in 68% of the treated effluent samples-meaning that these bacteria are continuously released into the receiving surface water. Temporal variation was observed for specific taxa in surface water, but not in wastewater samples.
Article
Radiology, Nuclear Medicine & Medical Imaging
C. Cano Rodriguez, E. Castaner Gonzalez, M. Andreu Magarolas, X. Gallardo Cistare, A. Gonzalez Lopez, O. Cuevas Lobato, M. Gallego Diaz
Summary: This study retrospectively analyzed the epidemiology and CT findings of nontuberculous mycobacterial lung infections in 131 patients and evaluated the outcomes of different treatment approaches. The results showed that the diagnosis of nontuberculous mycobacterial lung infections is complex, and the clinical and radiological findings are often nonspecific. Additionally, a significant percentage of patients may have concomitant infections with other microorganisms.
Article
Microbiology
Javier E. Canada-Garcia, Natalin Grippo, Eva Ramirez de Arellano, Veronica Bautista, Noelia Lara, Ana Maria Navarro, Teresa Cabezas, Nora Mariela Martinez-Ramirez, Silvia Garcia-Cobos, Jorge Calvo, Emilia Cercenado, Belen Aracil, Maria Perez-Vazquez, Jesus Oteo-Iglesias
Summary: This study analyzed the genomic and phenotypic characteristics of IMP-producing Enterobacterales (IMP-Ent) isolates circulating in Spain. The most frequently detected strains were IMP-8-producing Klebsiella pneumoniae (IMP-Kpn) and IMP-22-producing Enterobacter roggenkampii. However, some IMP-producing strains showed false negative results in EDTA or dipicolinic acid inhibition tests.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Elisa J. M. Raineri, Sandra Maass, Min Wang, Siobhan Brushett, Laura Palma M. Medina, Neus Sampol Escandell, Dania Altulea, Erwin Raangs, Anne de Jong, Elias Vera Murguia, Edward J. Feil, Alex W. Friedrich, Girbe Buist, Dorte Becher, Silvia Garcia-Cobos, Natacha Couto, Jan Maarten van Dijl
Summary: Goldylococcus aureus can invade the bloodstream through the gut, causing serious infections, and the mechanisms behind this process were not well understood. In this study, genome-wide association studies were conducted to compare the genetic sequences of gut and bloodstream isolates, along with proteomic characterization and infection models. The results showed that even isolates with the same sequence type can have genetic and proteomic differences. It was also found that bloodstream isolates did not necessarily possess higher virulence potential. The integrity of the gut epithelial layer may determine the invasiveness of staphylococci from the gut microbiome.
Article
Infectious Diseases
Javier E. Canada-Garcia, Eva Ramirez de Arellano, Miguel Jimenez-Orellana, Esther Viedma, Aida Sanchez, Almudena Alhambra, Jennifer Villa, Alberto Delgado-Iribarren, Veronica Bautista, Noelia Lara, Silvia Garcia-Cobos, Belen Aracil, Emilia Cercenado, Maria Perez-Vazquez, Jesus Oteo-Iglesias
Summary: During the COVID-19 pandemic, the number of ICU patients coinfected by nosocomial microorganisms increased. A study analyzed carbapenemase-producing Klebsiella pneumoniae (CP-Kpn) isolates from COVID-19 ICU patients and compared them to pre-pandemic populations. The study found similarities between the pandemic and pre-pandemic CP-Kpn populations, but there was an increase in VIM-1 isolates associated with atypical sequence types during the pandemic, suggesting the need for additional monitoring.
Article
Infectious Diseases
Antoni P. A. Hendrickx, Sylvia Debast, Maria Perez-Vazquez, Annelot F. Schoffelen, Daan W. Notermans, Fabian Landman, Cornelia C. H. Wielders, Javier E. Canada Garcia, Jacky Flipse, Angela de Haan, Sandra Witteveen, Marga van Santen-Verheuvel, Sabine C. de Greeff, Ed Kuijper, Leo M. Schouls
Summary: The genetic cluster of Enterobacter cloacae complex isolates collected in Dutch national surveillance from 2015-20 revealed a lineage producing carbapenemase, carrying multiple antibiotic resistance genes. The EclCluster-013 isolates, originating from three different hospitals in the Netherlands, belonged to the globally disseminated ST78 lineage and carried an IncH12 plasmid containing bla(VIM-1) carbapenemase and the novel mcr-9 colistin resistance gene. These MDR strains were susceptible to meropenem and colistin.
JAC-ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)