Review
Fisheries
Hien Van Doan, Mehdi Soltani, Einar Ringo
Summary: Gram-positive probiotic bacteria have been shown to be effective in preventing disease outbreaks in aquaculture by producing bioactive compounds that can inhibit potential pathogenic Gram-negative bacteria. They can also enhance host immune-physiological functions and improve disease resistance. Further research is needed to optimize probiotic dosages and understand the in vivo effects of Gram-positive probiotics.
Article
Microbiology
Xiao Zhang, Liming Tan, Pengwen Ouyang, Haiyan Ma, Jianqiao Peng, Ting Shi, Liangyi Xie
Summary: This study analyzed the prevalence and drug resistance of Gram-positive bacteria in a general tertiary-care hospital from 2014 to 2021. The results showed that the total number and species of Gram-positive bacteria increased continuously during this period. Streptococcus pneumoniae and Staphylococcus aureus were identified as the main causes of positive bacterial infections in the hospital. Additionally, the resistance rates of Enterococcus faecium to commonly used antibiotics significantly increased.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
Srishtee Arora, Jay Gordon, Magnus Hook
Summary: Collagens are the primary structural components of mammalian extracellular matrices, regulating tissue development, regeneration, and host defense. There are 28 genetically distinct collagens in humans, along with other unrelated human proteins containing a collagen domain. Gram-positive bacteria express cell surface proteins that bind to collagen and can be classified into different structural families.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Christine A. Pybus, Christina Felder-Scott, Victor Obuekwe, David E. Greenberg
Summary: The study compared the antimicrobial activity of Cefiderocol with seven commonly used antibiotics against multidrug-resistant pathogens, finding that Cefiderocol had potent inhibitory effects on biofilms of various pathogens, with consistently lower MIC90 values compared to other antibiotics.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Review
Public, Environmental & Occupational Health
Usman Abubakar, Menier Al-Anazi, Zainab Alanazi, Jesus Rodriguez-Bano
Summary: The COVID-19 pandemic has led to changes in the rate of multidrug resistant gram positive and gram negative bacteria. Studies have shown an increase in MRSA and VRE infection/colonization rates, while ESBL E. coli and ESBL Klebsiella pneumoniae rates have decreased. Overall, there has been an increase in the rate of multidrug resistant CRE infection/colonization during the pandemic, while the rate of CRPA infection has decreased.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Multidisciplinary Sciences
Tanshi Mehrotra, Dipasri Konar, Agila Kumari Pragasam, Shakti Kumar, Pradipta Jana, Prabhakar Babele, Deepjyoti Paul, Ayushi Purohit, Subhash Tanwar, Susmita Bakshi, Santanu Das, Jyoti Verma, Daizee Talukdar, Lekshmi Narendrakumar, Akanksha Kothidar, Sonali Porey Karmakar, Susmita Chaudhuri, Sujoy Pal, Kajal Jain, Chittur V. Srikanth, M. Jeeva Sankar, Krishnamohan Atmakuri, Ramesh Agarwal, Rajni Gaind, Mamatha Ballal, Nagamani Kammili, Rupak K. Bhadra, Thandavarayan Ramamurthy, G. Balakrish Nair, Bhabatosh Das
Summary: Microbes evolve rapidly through mutations and acquisition of mobile genetic elements (MGEs) associated with fitness traits like antimicrobial resistance (AMR). A multicentric study in India decoded genome sequences of bacterial pathogens causing sepsis, urinary tract infections, and respiratory infections. The study identified pathogen-specific AMR genes (ARGs) and MGEs, and differential abundance of resistance-associated proteins in the presence and absence of antibiotics, providing valuable insights for AMR diagnostics and antimicrobial therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Applied
Yuanyuan Wang, Yang Xue, Qianqian Bi, Dingkui Qin, Qizhen Du, Peng Jin
Summary: The study demonstrated that ECL-NPs have significant antibacterial efficiency against gram-positive bacteria, with the synergistic effect of low-dose lysozyme enhancing their inhibitory effects. Scanning electron microscopy showed that ECL-NPs could completely break down the cellular structures of bacteria, while 100% microbial inhibition was observed in fresh fruits treated with ECL-NPs for 15 days.
Article
Microbiology
Yuan Liu, Kangni Yang, Yuqian Jia, Jingru Shi, Ziwen Tong, Zhiqiang Wang
Summary: The study showed that thymine can effectively enhance the bactericidal activity of antibiotics against Gram-negative pathogens by activating bacterial metabolism and restoring susceptibility to antibiotics. This finding provides a universal strategy to overcome Gram-negative pathogens.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jaime E. Zlamal, Semen A. Leyn, Mallika Iyer, Marinela L. Elane, Nicholas A. Wong, James W. Wamsley, Maarten Vercruysse, Fernando Garcia-Alcalde, Andrei L. Osterman
Summary: Resistance to the broad-spectrum antibiotic ciprofloxacin is detected at high rates in various bacterial pathogens, with early resistance primarily caused by single mutations in DNA gyrase target genes. Further genetic alterations influence drug efflux mechanisms and secondary target genes, leading to increased resistance levels. The study highlights shared and unique evolutionary aspects across different species, providing insights for the development of novel antibiotics with reduced resistance tendencies.
Article
Infectious Diseases
Maria Di Pietrantonio, Lucia Brescini, Jennifer Candi, Morroni Gianluca, Francesco Pallotta, Sara Mazzanti, Paolo Mantini, Bianca Candelaresi, Silvia Olivieri, Francesco Ginevri, Giulia Cesaretti, Sefora Castelletti, Emanuele Cocci, Rosaria G. Polo, Elisabetta Cerutti, Oriana Simonetti, Oscar Cirioni, Marcello Tavio, Andrea Giacometti, Francesco Barchiesi
Summary: This retrospective study evaluated the efficacy of ceftazidime/avibactam treatment in 81 patients with Gram-negative infection. The results showed that ceftazidime/avibactam exhibited high efficacy in treating multidrug-resistant Gram-negative pathogens with limited treatment options.
Review
Microbiology
Ananda Tiwari, Paula Kurittu, Ahmad Al-Mustapha, Viivi Heljanko, Venla Johansson, Ocean Thakali, Shyam Kumar Mishra, Kirsi-Maarit Lehto, Anssi Lipponen, Sami Oikarinen, Tarja Pitkanen, Annamari Heikinheimo
Summary: Infectious diseases caused by antibiotic-resistant bacteria pose a serious threat to human and animal health. Active surveillance of antibiotic-resistant bacteria using a comprehensive one-health approach can help reduce their emergence and spread, reduce associated economic impacts, and guide antimicrobial stewardship programs. Wastewater surveillance of antibiotic-resistant bacteria provides composite samples for monitoring the total population, making it a promising tool for monitoring these pathogens. However, the clinical utility, sensitivity, and uniformity of wastewater surveillance of antibiotic-resistant bacteria remains poorly understood, especially in relation to clinical evidence in communities.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Rodrigo E. Mendes, S. J. Ryan Arends, Jennifer M. Streit, Ian Critchley, Nicole Cotroneo, Mariana Castanheira
Summary: The occurrence of urinary-tract Enterobacterales pathogens producing ESBL enzymes in community and nosocomial settings continues to increase, posing challenges to the management of UTI. Tebipenem, a broad-spectrum carbapenem, showed promising activity against UTI pathogens. However, there was variability in susceptibility patterns and resistance rates among different regions and isolates.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Kamaleldin B. Said, Ahmed Alsolami, Amany M. Khalifa, Nuha A. Khalil, Soha Moursi, Ehab Rakha, Abuzar Osman, Musleh Rashidi, Taha E. Taha, Abdelhafiz Bashir, Safia Moussa, Ahmed Al Jadani, Hatem Nagi, Mohammad Kuddus, Obaid M. Alrashedi, Amar S. Alharbi, Abdulrahman Alfaraj, Roba Mustafa
Summary: This study aimed to objectively assess Staphylococcus aureus and other Gram-positive pathogens recovered from clinical infections in the Ha'il region using molecular detections, antimicrobial resistances profiles, patient demographics, and disease patterns. The results showed that Staphylococcus aureus was the most frequent multidrug resistant isolate, primarily causing surgical infections. Staphylococcus epidermidis was the second most common species, mainly causing bloodstream infections. Enterococcus faecalis ranked third, with the majority of infections being bloodstream and urinary-tract infections in seniors.
MICROBIOLOGY RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Sarabjot Kaur, Rushikesh Tambat, Vikas Pathania, Hemraj Nandanwar, Sanjeev Soni
Summary: This study demonstrates that photo-thermally enhanced triangular silver nanoplates have strong antimicrobial efficacy and can eradicate various pathogens in a short period of time, which is significant for a wide range of antibacterial and antifungal applications.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Review
Immunology
Flora Cruz-Lopez, Adrian Martinez-Melendez, Rayo Morfin-Otero, Eduardo Rodriguez-Noriega, Hector J. Maldonado-Garza, Elvira Garza-Gonzalez
Summary: Infections caused by Gram-negative multi-drug resistant (MDR) bacterial species are difficult to treat due to widespread antibiotic resistance. New antibiotics and combinations with existing drugs have shown potential in treating these infections, but there are reports of resistance to these treatments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)