Article
Virology
Samuel Santos Pereira, Robert Andreata-Santos, Maria Fernanda de Castro-Amarante, Alexia Adrianne Venceslau-Carvalho, Natiely Silva Sales, Mariangela de Oliveira Silva, Rubens Prince dos Santos Alves, Patricia Jungmann, Luis Carlos de Souza Ferreira
Summary: This study developed a specific dengue virus IgG ELISA based on a multi-epitope NS1-based antigen for antibody detection. The results showed that the method can specifically recognize human serum infected with dengue virus, but showed no or reduced reactivity with subjects infected with Zika virus.
Article
Infectious Diseases
Samuel Santos Pereira, Robert Andreata-Santos, Lennon Ramos Pereira, Camila Pereira Soares, Alvina Clara Felix, Patricia de Mello Jungmann Cardoso de Andrade, Edison Luis Durigon, Camila Malta Romano, Luis Carlos de Souza Ferreira
Summary: The study aimed to achieve greater specificity in DENV serological tests by using a recombinant antigen (Delta NS1) to differentiate DENV and ZIKV. The Delta NS1-based DENV IgG ELISA detected anti-DENV NS1 IgG without cross-reactivity with ZIKV-positive samples, with a sensitivity of 82% and specificity of 93%. This method may be particularly useful for serological analyses in endemic regions for both DENV and ZIKV transmission.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Tanapan Prommool, Pongpawan Sethanant, Narodom Phaenthaisong, Nattaya Tangthawornchaikul, Adisak Songjaeng, Panisadee Avirutnan, Dumrong Mairiang, Prasit Luangaram, Chatchawan Srisawat, Watchara Kasinrerk, Sirijitt Vasanawathana, Kanokwan Sriruksa, Wannee Limpitikul, Prida Malasit, Chunya Puttikhunt
Summary: The study modified the stNS1-ELISA method by introducing biotinylated serotype-specific antibodies and a new DENV2-specific antibody, resulting in a significant improvement in sensitivity for dengue virus serotype detection. The modified assay is efficient for early dengue diagnosis and epidemiological studies.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Erandi Munasinghe, Maheshi Athapaththu, Wimaladharma Abeyewickreme
Summary: Synthetic peptides were designed and synthesized as antigens for antibody production to detect dengue infection. Monoclonal antibodies generated from immunized mice showed significant reactivity with dengue NS1 protein, enabling the detection of dengue infection in urine samples.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Medicine, General & Internal
Somphavanh Somlor, Ludovic Brossault, Marc Grandadam
Summary: The study evaluated the performance of three prototype assays developed for the VIDAS(R) automated platform in detecting dengue virus infection. The results showed that the VIDAS(R) NS1 assay had the best overall agreement with competitor ELISA, while the IgM and IgG assays had moderate agreement.
Article
Medicine, General & Internal
Alice F. Versiani, Antoinette Kabore, Ludovic Brossault, Loic Dromenq, Thayza M. I. L. dos Santos, Bruno H. G. A. Milhim, Cassia F. Estofolete, Assana Cisse, Pegdwende Abel Sorgho, Florence Senot, Marie Tessonneau, Serge Diagbouga, Mauricio L. Nogueira
Summary: This study evaluated the performance of the VIDAS(R) dengue immunoassays in diagnosing dengue virus infections. The results showed that these immunoassays have high accuracy and stability in detecting NS1 antigen and anti-DENV IgM and IgG antibodies. They are suitable for the accurate diagnosis of dengue infections in endemic regions.
Article
Virology
Po-Kai Chen, Jyun-Hao Chang, Liang-Yin Ke, Jun-Kai Kao, Chang-Hua Chen, Rei-Cheng Yang, Teruki Yoshimura, Etsuro Ito, Jih-Jin Tsai
Summary: A detection method for the NS1 protein based on an ultrasensitive ELISA was developed and evaluated, showing high sensitivity and specificity. This low-cost and easy-to-use method has great potential for clinical diagnosis of DENV, especially in low-income countries.
Article
Medicine, General & Internal
Jedhan Ucat Galula, Gielenny M. Salem, Raul V. Destura, Roland Remenyi, Day-Yu Chao
Summary: The study compared the diagnostic performances of two NS1-based ELISA methods with an E-based VLP ELISA in determining dengue serostatus, finding that NS1-based methods have comparable accuracies and could effectively assist clinicians in assessing vaccine eligibility.
Article
Chemistry, Analytical
Bassam Bachour Junior, Marina Ribeiro Batistuti, Aline Sanches Pereira, Elisa Maria de Sousa Russo, Marcelo Mulato
Summary: An electrochemical biosensor for NS1 detection was developed in this study by optimizing the molar ratio between aptamers and MCH, achieving high sensitivity and low detection limits for NS1 detection in the early stages of disease. By adding bovine serum albumin to stabilize NS1, the sensor accurately detected NS1 from different Dengue serotypes.
Article
Infectious Diseases
Anisa Ghosh, Soumi Sukla, Himadri Nath, Rajdeep Saha, Abhishek De, Subhajit Biswas
Summary: NS1 mutations directly impact the detection of dengue virus through NS1 ELISA, leading to decreased sensitivity. Specific NS1 gene mutations are associated with reduced NS1 production and secretion, which correlates with NS1 ELISA-negative results. Recently circulating dengue virus strains may not be detected by current NS1 ELISA despite being RNA-positive. Further research is needed to define more NS1 mutations in clinical samples to optimize the sensitivity of diagnostic kits.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Pei-Yin Lim, Appanna Ramapraba, Thomas R. Loy, Angeline Rouers, Tun-Linn Thein, Yee-Sin A. Leo, Dennis A. Burton, Katja A. Fink, Cheng-I A. Wang
Summary: In this study, four antibodies against NS1 were characterized and their binding specificities were determined. Two antibodies (A2 and D6) recognized overlapping epitopes on NS1, while another antibody (D8) recognized a distinct epitope. A dengue-specific capture ELISA was developed using one of the antibodies (Den3) as the capture antibody and another antibody (D8) as the detecting antibody. This assay successfully detected NS1 from all tested dengue virus strains and dengue-infected patients.
Article
Virology
Brandon E. K. Tan, Michael R. Beard, Nicholas S. Eyre
Summary: In this study, random point mutagenesis was used to identify residues within NS1 that are essential for its secretion. Ten point mutations were identified that corresponded with impaired NS1 secretion. Additional studies on two of these mutants, V220D and A248V, revealed that they prevented viral RNA replication and disrupted NS1 processing or maturation.
Article
Multidisciplinary Sciences
Linh Tran, Sangkwon Park
Summary: A highly sensitive immunosensor using quantum dots and streptavidin was developed for detecting the dengue biomarker NS1, offering a fast, convenient, and sensitive approach. However, interference in human plasma serum solution was observed, suggesting the need for further solutions.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Fahimeh Farokhinejad, Junrong Li, Leon E. Hugo, Christopher B. Howard, Alain Wuethrich, Matt Trau
Summary: This study reports an innovative nanobased immunoassay platform for the early, specific, and ultrasensitive detection of DENV2 virus in infected mosquitoes. The platform uses nanomaterials and yeast affinity bionanofragments to enhance the sensitivity and specificity of detection, and employs electrohydrodynamically driven nanomixing to reduce nonspecific interactions. These nanotechnologies provide a significant innovation for preventing the transmission of DENV2.
ANALYTICAL CHEMISTRY
(2022)
Article
Immunology
Prajakta Kulkarni, Shripad Taklikar, Dilip Turbadkar
Summary: The study aimed to compare the performance of STANDARD F Dengue NS1 Ag FIA SD Biosensor and ELISA for diagnosing dengue in patients at a tertiary care hospital. The results showed that the FIA test results were comparable to the gold standard ELISA test in terms of sensitivity, specificity, positive predictive value, and negative predictive value. The FIA test provided rapid results and could be used routinely. The Cutoff Index (COI) value of FIA was inversely related to the platelet count, which could indicate the severity of the disease.
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Immunology
Silvana A. Vielma, Richard L. Klein, Corinne A. Levingston, M. Rita I. Young
INTERNATIONAL IMMUNOPHARMACOLOGY
(2013)
Article
Endocrinology & Metabolism
Silvana A. Vielma, Richard L. Klein, Corinne A. Levingston, M. Rita I. Young
Article
Microbiology
Elizabeth McMahon, Liliana Encinales, Carlos Navarro Encinales, Silvana Vielma, Nelly Pacheco, Lil Geraldine Avendano Echavez, Sandra Acosta Rodriguez, Milena Calderon, Silvia Encinales Sanabria, Lorena Encinales Sanabria, Ericka Serrano Bernal, Andres Gonzalez Coba, Dennys Jimenez, Gary Simon, Aileen Y. Chang
Article
Public, Environmental & Occupational Health
Juan C. Gabaldon-Figueira, Siham Salmen, Nubia Silva, Betania Mancilla, Silvana Vielma
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
(2020)
Article
Multidisciplinary Sciences
Ricardo A. Strauss, Laura Herrera-Leon, Ana C. Guillen, Julio S. Castro, Eva Lorenz, Ana Carvajal, Elizabeth Hernandez, Trina Navas, Silvana Vielma, Neiris Lopez, Maria G. Lopez, Lisbeth Aurenty, Valeria Navas, Maria A. Rosas, Tatiana Drummond, Jose G. Martinez, Erick Hernandez, Francis Bertuglia, Omaira Andrade, Jaime Torres, Juergen May, Silvia Herrera-Leon, Daniel Eibach
Summary: In 2016, Venezuela experienced a large diphtheria outbreak that persisted until 2019. The outbreak primarily affected individuals aged 10 to 19, with males showing a higher case fatality rate compared to females. The outbreak involved different strains of Corynebactrium suggesting vaccination coverage issues and inadequate control strategies.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Luis Tellez, Elvia Michelli, Jose Andres Mendoza, Silvana Vielma, Maria-Eugenia Noguera, Diana Callejas, Maria Cavazza, Maria Correnti
ECANCERMEDICALSCIENCE
(2015)
Article
Medicine, Research & Experimental
Erick Hernandez, Maria Araque, Ysheth Millan, Beatriz Millan, Silvana Vielma
INVESTIGACION CLINICA
(2014)
Article
Oncology
Silvana A. Vielma, Richard L. Klein, Corinne A. Levingston, M. Rita I. Young
ANTICANCER RESEARCH
(2013)
Article
Medicine, Research & Experimental
Maria Odreman-Macchioli, Silvana Vielma, Daniel Atchley, Guillermo Comach, Alvaro Ramirez, Saberio Perez, Luis Tellez, Beatriz Quintero, Erick Hernandez, Maritza Munoz, Jose Mendoza
INVESTIGACION CLINICA
(2013)
Article
Medicine, Research & Experimental
Elvia Michelli, Luis Tellez, Jose-Andres Mendoza, Claudia Juergensen, Maritza Munoz, Saberio Perez, Noraida Mosqueda, Erick Hernandez, Maria-Eugenia Noguera, Diana Callejas, Maria Correnti, Maria-Eugenia Cavazza, Silvana Vielma
INVESTIGACION CLINICA
(2011)
Article
Biochemistry & Molecular Biology
SA Vielma, M Mironova, JR Ku, MF Lopes-Virella
JOURNAL OF LIPID RESEARCH
(2004)
Article
Cardiac & Cardiovascular Systems
SA Vielma, G Krings, MF Lopes-Virella
CIRCULATION RESEARCH
(2003)
Article
Immunology
S Vielma, G Virella, AJ Gorod, MF Lopes-Virella
CLINICAL IMMUNOLOGY
(2002)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
