Article
Microbiology
Xuerui Bao, Ellen Goeteyn, Aurelie Crabbe, Tom Coenye
Summary: D,L-malate enhances ciprofloxacin activity against P. aeruginosa, improving antibiotic therapy in infection environments. The choice of model system influences the outcome of the experiment, emphasizing the importance of using models that closely mimic the in vivo environment. This study confirms the potential of D,L-malate to enhance ciprofloxacin activity against P. aeruginosa-associated infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Carlos F. Amabile-Cuevas
Summary: Biofilm formation by macrolide-resistant Staphylococcus aureus isolates can be induced by macrolides, especially in the early stages, suggesting that the empirical use of macrolides against infections caused by resistant S. aureus strains could lead to clinical failure or even enhance biofilm formation, making further treatment difficult.
Article
Critical Care Medicine
Eleni Karakike, Brendon P. Scicluna, Maria Roumpoutsou, Ioannis Mitrou, Niki Karampela, Athanasios Karageorgos, Konstantinos Psaroulis, Eleni Massa, Achillefs Pitsoulis, Panagiotis Chaloulis, Evanthia Pappa, Irene T. Schrijver, Frantzeska Frantzeskaki, Malvina Lada, Nicolas Dauby, David De Bels, Ioannis Floros, Souzana Anisoglou, Eleni Antoniadou, Maria Patrani, Glykeria Vlachogianni, Eleni Mouloudi, Anastasia Antoniadou, David Grimaldi, Thierry Roger, W. Joost Wiersinga, Iraklis Tsangaris, Evangelos J. Giamarellos-Bourboulis
Summary: Clarithromycin did not reduce mortality in patients with sepsis with respiratory and multiple organ dysfunction. Clarithromycin was associated with lower sepsis recurrence, possibly through a mechanism of immune restoration.
Article
Medicine, Research & Experimental
Raghad K. Aldhahri, Shahad G. Gabb, Ohoud A. Shoaib, Reem M. Almadani, Khalid Eljaaly, Abrar K. Thabit
Summary: This study compared the effectiveness and safety of a combination of doxycycline with a beta-lactam and a combination of a macrolide with a beta-lactam in the treatment of non-severe community-acquired pneumonia. The results showed that there was no significant difference in clinical cure rate, time to clinical stability, and length of stay between the two groups. However, the combination of doxycycline with a beta-lactam had a better safety profile.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
X-F Li, H-Q Shi, Y. Liang, J. Li, B. Jiang, G-B Song
Summary: In this study, it was discovered that 75.5% of CRPA isolates were MDR, and biofilm formation and efflux pumps may be the mechanisms of drug resistance in CRPA isolates. The expression of mexA gene was significantly higher in biofilm producers compared to planktonic bacteria.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2022)
Review
Clinical Neurology
Leonard P. Rybak, Vickram Ramkumar, Debashree Mukherjea
Summary: This review summarizes the ototoxicity of non-aminoglycoside antibiotics, discussing antibiotics such as capreomycin, macrolides, and vancomycin that have been associated with hearing loss in animal studies and in patients, especially in neonates. It emphasizes the importance of being aware of the ototoxic potential of these antibiotics, given the extensive focus on the ototoxicity of aminoglycoside antibiotics in the literature.
FRONTIERS IN NEUROLOGY
(2021)
Article
Microbiology
Xuerui Bao, Mona Bove, Tom Coenye
Summary: The high tolerance of Pseudomonas aeruginosa biofilms in respiratory tract infections in cystic fibrosis contributes to the failure of antibiotic therapy. This study identified carbon sources that could enhance the inhibiting and/or eradicating activity of tobramycin, ciprofloxacin, and ceftazidime against P. aeruginosa PAO1 biofilms. The mechanisms underlying the enhanced biofilm eradicating activity were strain-dependent.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Devin Sindeldecker, Shaurya Prakash, Paul Stoodley
Summary: This study analyzed the zone of killing (ZOK) in Pseudomonas aeruginosa biofilms using both in vitro and in silico methods. The findings revealed that achieving a specific antibiotic concentration versus time constraint (AUC) could completely eradicate bacteria in the biofilms. This suggests that appropriate antibiotic concentrations and treatment duration may be effective against antibiotic-resistant and -tolerant biofilm infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Ecology
Thomas James O'Brien, Wendy Figueroa, Martin Welch
Summary: The presence of diverse polymicrobial communities in the airways of individuals with cystic fibrosis can hinder the effectiveness of antibiotic treatment. A study showed that growth in a polymicrobial environment protects the target microorganism from the effects of specific antimicrobial agents. This decreased efficacy is found to have both physiological and genetic components.
Article
Multidisciplinary Sciences
Uthaibhorn Singkham-In, Wichit Thaveekarn, Jureeporn Noiphrom, Orawan Khow, Surada Ponwaranon, Jiraphorn Issara-Amphorn, Visith Sitprija, Asada Leelahavanichkul
Summary: Venoms from king cobra and green pit viper showed broad antibacterial spectrum, but king cobra venom had stronger anti-biofilm activities against P. aeruginosa. A purified amino acid oxidase from king cobra venom reduced biofilm mass and disrupted preformed biofilms, making it a potential agent against Pseudomonas biofilms. Further studies are needed to explore its potential.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Fernanda Jimenez Otero, Dianne K. Newman, Leonard M. Tender
Summary: Biofilms provide a protective environment but also present challenges to the cells living within them. Pseudomonas aeruginosa overcomes oxygen limitation by secreting redox active phenazines, which act as electron shuttles. Blocking the re-oxidation of one of these electron shuttles, pyocyanin, decreases cell survival within biofilms and enhances the effect of gentamicin in killing cells. The study highlights the importance of redox cycling of electron shuttles in P. aeruginosa biofilms.
Article
Microbiology
Firat Kaya, Jacqueline P. Ernest, Katherine LoMauro, Martin Gengenbacher, Abdeldjalil Madani, Wassihun Wedajo Aragaw, Matthew D. Zimmerman, Jansy P. Sarathy, Nadine Alvarez, Isaac Daudelin, Han Wang, Faye Lanni, Danielle M. Weiner, Laura E. Via, Clifton E. Barry, Kenneth N. Olivier, Thomas Dick, Brendan K. Podell, Radojka M. Savic, Veronique Dartois
Summary: This study found that the rabbit model of active tuberculosis reproduces key features of human NTM-PD and provides an acceptable surrogate model for studying antibiotic penetration and efficacy. The research also revealed that the clinical activity of clarithromycin is limited by pharmacodynamic factors.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Amal H. Yahya, Sophie R. Harston, William L. Colton, Matthew T. Cabeen
Summary: Pseudomonas aeruginosa is a common pathogen that infects hospitalized patients and individuals with cystic fibrosis. This species is known for forming biofilms, which provide extra protection to the cells and make infections difficult to treat. The deletion of the gene PA14_16550 reduces biofilm formation, and further investigations have identified other genes that regulate biofilm matrix production.
MICROBIOLOGY SPECTRUM
(2023)
Review
Biotechnology & Applied Microbiology
Maria del Mar Cendra, Eduard Torrents
Summary: This article discusses the traits of Pseudomonas aeruginosa biofilms and the clinical challenges they pose, focusing on the bacterium's ability to coexist and interact with other microorganisms. It explores infections caused by P. aeruginosa single and polymicrobial biofilms, as well as current models used to recreate them in laboratory conditions. Antimicrobial and antibiofilm strategies developed against P. aeruginosa mono and multispecies biofilms are also detailed.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Multidisciplinary Sciences
Chelsey M. VanDrisse, Rosalie Lipsh-Sokolik, Olga Khersonsky, Sarel J. Fleishman, Dianne K. Newman
Summary: This study demonstrates that by engineering the structure of PodA protein and combining it with tobramycin, the killing effect on P. aeruginosa can be enhanced, especially on biofilms that are resistant to conventional antibiotic treatment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
K. Rainha, Debora Lins, R. F. Ferreira, C. L. Costa, B. Penna, B. T. Endres, K. W. Garey, R. M. C. P. Domingues, E. O. Ferreira
Summary: Clostridioides difficile has been identified as one of the primary causes of nosocomial diarrhea and pseudomembranous colitis in humans and other mammals after the use of broad-spectrum antibiotics. A case of C. difficile infection (CDI) in a 13-year-old male dog is described in Rio de Janeiro, Brazil.
Article
Immunology
Kevin W. Garey, Jacob McPherson, An Q. Dinh, Chenlin Hu, Jinhee Jo, Weiqun Wang, Chris K. Lancaster, Anne J. Gonzales-Luna, Caroline Loveall, Khurshida Begum, M. Jahangir Alam, Michael H. Silverman, Blake Hanson
Summary: This study validates the effectiveness of ibezapolstat in treating adult patients with Clostridioides difficileinfection. The results show sustained clinical cure and beneficial effects on the microbiome and bile acids.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Microbiology
Chenlin Hu, Nicholas D. Beyda, Kevin W. Garey
Summary: The human microbiome project has revolutionized our understanding of the interaction between commensal microbes and human health. In this study, a simple and fast HPLC method was validated for quantitative fecal vancomycin analysis and the reproducibility of results were tested, establishing sample condition standards for quantitative HPLC studies on vancomycin pharmacokinetics with the human microbiome.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Jacob McPherson, Chenlin Hu, Khurshida Begum, Weiqun Wang, Chris Lancaster, Anne J. Gonzales-Luna, Caroline Loveall, Michael H. Silverman, M. Jahangir Alam, Kevin W. Garey
Summary: This study used a functional and metagenomic approach to predict the potential anti-CDI recurrence effect of ibezapolstat. The results from the clinical study showed that ibezapolstat demonstrated beneficial changes in microbiome and bile acid concentrations, suggesting a lower risk of CDI recurrence compared to vancomycin.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Jinhee Jo, Truc T. Tran, Nicholas D. Beyda, Debora Simmons, Joshua A. Hendrickson, Masaad Saeed Almutairi, Faris S. Alnezary, Anne J. Gonzales-Luna, Edward J. Septimus, Kevin W. Garey
Summary: This study aimed to assess the use of echinocandin at hospital discharge and develop a transition of care model for patients with invasive candidiasis (IC). The study found a significant use of echinocandin at discharge, with almost half of the discharged patients receiving outpatient echinocandin therapy. Osteomyelitis, other deep-seated infection, and non-home discharge location were independent predictors for outpatient echinocandin use. The developed discharge model may assist in facilitating smoother and earlier hospital discharges.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Arnaldo L. Colombo, Joao N. De Almeida, Russell E. Lewis, Dimitrios P. Kontoyiannis
Summary: This article reviews the viewpoint of Slavin et al. that comprehensively addresses the clinical factors driving changes in antifungal therapy for invasive aspergillosis. The authors highlight the lack of quality data to support clinical decision-making in managing patients with invasive aspergillosis.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Review
Microbiology
Chenlin Hu, Kevin W. Garey
Summary: Clostridioide difficile is the leading cause of diarrhea disease worldwide and is considered an urgent threat pathogen by CDC. Mammalian models are commonly used to study C. difficile infection, but alternative nonmammalian models, such as great wax worm, nematode, fruit fly, and zebrafish, have been explored due to cost, throughput capacity, and ethical concerns. This review provides a comprehensive summary of the use of nonmammalian models in studying CDI, including its infection mechanism, pathogenicity, colonization, host immunity, and therapy. The translational outcomes and strengths and weaknesses of each nonmammalian model are discussed.
Article
Infectious Diseases
M. Hoenigl, R. Lewis, F. L. van de Veerdonk, P. E. Verweij, O. A. Cornely
Summary: Advances in medicine have increased the number of people with compromised immune systems who are susceptible to fungal infections. Severe fungal infections are becoming more common in intensive care units and affect various populations. Treatment approaches are changing, and new drugs are increasing the risk of fungal infections. Rare fungal and yeast infections are also increasing, and diagnosis remains challenging. Continuous surveillance and personalized treatment strategies are needed to address these changes in fungal infections.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Pharmacology & Pharmacy
Ahmed Zaki, Anne J. Gonzales-Luna, Nicholas D. Beyda, Todd Lasco, Kevin W. Garey
Summary: This study evaluated the clinical outcomes of micafungin based on population-predicted pharmacokinetic/pharmacodynamic factors and susceptibility. MIC and AUC/MIC breakpoints derived from CART analysis predicted patient mortality and treatment failure for certain Candida species. These results support further PK/PD studies to optimize echinocandin dosing and improve patient outcomes.
Review
Gastroenterology & Hepatology
Anne J. Gonzales-Luna, Travis J. Carlson, Kevin W. Garey
Summary: Human gut microbiota play a critical role in the development and recovery of Clostridioides difficile infection (CDI). Antibiotics, although necessary for CDI treatment, can further disrupt the gut microbiota, leading to dysbiosis and complicating recovery. Various microbiota-based treatment approaches, such as fecal microbiota transplantation (FMT) and newly approved live biotherapeutic products, have been developed to address disease- and treatment-associated dysbiosis and improve cure rates. This review aims to discuss the changes in the gut microbiome associated with CDI and the effectiveness of different microbiota-based treatments.
Review
Pharmacology & Pharmacy
Erin K. K. McCreary, Matthew R. R. Davis, Navaneeth Narayanan, David R. R. Andes, Dario Cattaneo, Robbie Christian, Russell E. E. Lewis, Kevin M. M. Watt, Nathan P. P. Wiederhold, Melissa D. D. Johnson
Summary: Triazole antifungals are commonly used in clinical practice to prevent or treat invasive fungal infections. Therapeutic drug monitoring is necessary due to their variable pharmacokinetics and established relationships between exposure and response.
Article
Immunology
Joseph Pechacek, Taura Webb, Elise M. N. Ferre, Monica M. Schmitt, Thomas DiMaggio, Dale Kobrin, Suraj Rajasimhan, Ben Colton, Russell E. Lewis, David Andes, Alejandro Herrera, Dima Hammoud, Seyedmojtaba Seyedmousavi, Sarfaraz Hasni, Jonathan Bolanos, Behdad Afzali, Michail S. Lionakis
Summary: This article reports a case of successful treatment of a patient with Paecilomyces variotii pneumonia and concomitant lupus nephritis using posaconazole and echinocandin-bridged interruptions to allow for cyclophosphamide therapy. The treatment regimen was well-tolerated without cyclophosphamide toxicity and achieved improvements in both fungal pneumonia and renal function.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Immunology
Kevin W. Garey, Erik R. Dubberke, Amy Guo, Adam Harvey, Min Yang, Viviana Garcia-Horton, Mirko Fillbrunn, Hongjue Wang, Glenn S. Tillotson, Lindy L. Bancke, Paul Feuerstadt
Summary: In this study, the disease-specific health-related quality of life (HRQL) in patients with rCDI treated with fecal microbiota was evaluated. The results demonstrated that patients treated with RBL had more improvements in the mental domain compared to those receiving placebo.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Russell E. Lewis, Marta Stanzani
Summary: This review provides an overview of potential drug-drug interactions (DDIs) between small-molecule kinase inhibitors (SMKIs) and triazole antifungals in the treatment of acute myeloid leukemia (AML). Recent findings suggest the need for tailored approaches to manage these interactions, considering patient-specific risks and alternative treatments. The use of SMKIs has revolutionized AML treatment, but careful management of DDIs with triazole antifungals is necessary.
CURRENT FUNGAL INFECTION REPORTS
(2023)
Review
Hematology
Jannik Stemler, Nick de Jonge, Nicole Skoetz, Janos Sinko, Roger J. Bruggemann, Alessandro Busca, Ronen Ben-Ami, Zdenek Racil, Vanessa Piechotta, Russell Lewis, Oliver A. Cornely
Summary: Antifungal prophylaxis is strongly recommended during remission induction chemotherapy for patients with acute myeloid leukemia. However, the potential drug-drug interactions and risk-benefit ratio of novel targeted agents have not been assessed in clinical trials. This study developed up-to-date recommendations on antifungal prophylaxis and management of pharmacokinetic drug-drug interactions with triazole antifungals.
LANCET HAEMATOLOGY
(2022)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
