Article
Microbiology
Satomi Takei, Yu Jie Lu, Mari Tohya, Shin Watanabe, Shigeki Misawa, Yoko Tabe, Takashi Miida, San Mya, Htay Htay Tin, Tatsuya Tada, Teruo Kirikae
Summary: This study demonstrates the spread of carbapenem-resistant K. pneumoniae strains in medical settings in Myanmar. Specifically, plasmid genes encoding NDMs and 16S rRNA methylases have been spreading in high-risk clones of K. pneumoniae.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Wenjian Liao, Liang De Wang, Dan Li, Fang-ling Du, Dan Long, Yang Liu, OonTek Ng, Wei Zhang
Summary: This study found a high prevalence of 16S rRNA methylase genes in CR-hvKP clinical isolates, especially in ST11. Continuous monitoring of the epidemiology of these 16S rRNA methylase-producing CR-hvKP is critical, while simultaneously minimizing potential risks from aminoglycoside-resistant CR-hvKP.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Melanie Roch, Roberto Sierra, Kirsty Sands, Willames M. B. S. Martins, Jacques Schrenzel, Timothy R. Walsh, Ana C. Gales, Diego O. Andrey
Summary: The study found that a significant portion of KPC-producing Klebsiella pneumoniae isolates in a Brazilian hospital exhibited resistance to common aminoglycosides like gentamicin and amikacin, with the presence of the rmtB gene in 30% of the strains. The rmtB gene was mainly found in sequence type 258 (ST258) and a novel ST16 clone, carried on large IncC plasmids. Furthermore, most isolates remained susceptible to the veterinary approved drug apramycin. The dissemination of rmtB in an endemic setting for KPC-producing clones is concerning, as it confers resistance to clinically available aminoglycosides, including the novel plazomicin.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Review
Infectious Diseases
Weiwei Yang, Fupin Hu
Summary: Antimicrobial resistance is a major concern in modern healthcare, with the emergence of 16S-RMTase encoding genes being a significant mechanism for aminoglycoside resistance. The transferable plasmids contribute to the widespread spread of resistance genes among bacteria, posing challenges for the treatment of infectious diseases and infection control.
Article
Fisheries
Yaxin Wu, Yibin Yang, Huifeng Dang, Hehe Xiao, Wenji Huang, Zhao Jia, Xin Zhao, Kangyong Chen, Ning Ji, Jiahong Guo, Zhiwei Qin, Junya Wang, Jun Zou
Summary: The study isolated a pathogenic strain of Klebsiella pneumoniae from a diseased spotted gar and explored its virulence and drug resistance. Results showed that fish infected with K. pneumoniae exhibited decreased immune cells and tissue inflammation.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Microbiology
Tatsuya Tada, Satoshi Oshiro, Shin Watanabe, Mari Tohya, Tomomi Hishinuma, Thi Thi Htoon, Htay Htay Tin, Teruo Kirikae
Summary: The study describes two clinical isolates of Klebsiella pneumoniae coharbouring bla(IMP-1), armA and mcr-10, which are resistant to carbapenems and aminoglycosides. Whole-genome analysis revealed the presence of bla(NDM-1), armA, and mcr-10 in these isolates.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Le Wang, Wenfu Zheng, Sixiang Li, Leni Zhong, Xingyu Jiang
Summary: This study presents the modification of gold nanoparticles with aminophenol, which demonstrates a broad spectrum of antibacterial effects and strong efficacy against multidrug-resistant bacteria. The mechanisms of action include damaging bacterial cell walls and inhibiting bacterial protein synthesis. In vivo experiments also show promising results in treating abdominal bacterial infections.
Review
Pharmacology & Pharmacy
Xinqian Ma, Wen Xi, Deqing Yang, Lili Zhao, Wenyi Yu, Yukun He, Wentao Ni, Zhancheng Gao
Summary: Bacteria can develop increased sensitivity to certain antibiotics after acquiring resistance to another antibiotic, known as collateral sensitivity. Exploiting collateral sensitivity in treatment design could effectively suppress or reverse resistance evolution.
DRUG RESISTANCE UPDATES
(2023)
Article
Infectious Diseases
Toshihiro Takahashi, Tatsuya Tada, Shovita Shrestha, Tomomi Hishinuma, Jatan B. Sherchan, Mari Tohya, Teruo Kirikae, Jeevan B. Sherchand
Summary: This study aimed to determine the genetic and epidemiological properties of carbapenem-resistant Pseudomonas aeruginosa strains isolated from hospitals in Nepal. The findings revealed that these strains carried various carbapenemase and 16S rRNA methylase genes, belonging to different sequence types. These results suggest the spread of carbapenem-resistant P. aeruginosa in medical settings in Nepal.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Medical Laboratory Technology
Z. Demirbas, H. Selcuk Ozger, Serap Suzuk-Yildiz, Zekiye Bakkaloglu, Murat Dizbay
Summary: This study evaluated the in vitro activities of plazomicin, amikacin, gentamicin, and tobramycin against carbapenem resistant Klebsiella pneumoniae (CR-Kp) isolates. The most commonly detected carbapenemase enzyme was OXA-48. Plazomicin showed increased efficacy compared to other aminoglycosides and may be an effective treatment option for CR-Kp isolates.
CLINICAL LABORATORY
(2022)
Article
Microbiology
Yushan Pan, Tengli Zhang, Lijie Yu, Zhiyong Zong, Shiyu Zhao, Ruichao Li, Qianqian Wang, Li Yuan, Gongzheng Hu, Dandan He
Summary: This study aimed to investigate the role of IS1294 in plasmid reorganization and analyze biological characteristics of cointegrates derived from different daughter plasmids. Through various experiments, the genetic profiles of plasmids in Escherichia coli strain C21 and its transconjugants were characterized, shedding light on the formation and evolution of cointegrate plasmids under different drug selection pressures. This study provides insight into the significance of IS1294 in the formation of cointegrates and the common features of IS1294-driven cointegration of plasmids.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
Camilla Smoglica, Muhammad Farooq, Fausto Ruffini, Fulvio Marsilio, Cristina Esmeralda Di Francesco
Summary: This study compared the microbiome composition and abundance of antimicrobial resistance genes in broiler litter samples from conventional and antibiotic-free flocks. The results suggest that antibiotic use can influence the frequency of resistance determinants and the microbial community in poultry flocks.
Article
Biochemistry & Molecular Biology
M. Mohamed Mahroop Raja, N. Reehana, A. Asrar Ahamed, A. Fasila Begum
Summary: The study observed the gradual increase of multiple antibiotic resistances in gram negative organisms such as Klebsiella pneumoniae and Proteus vulgaris, which are major causes of infection worldwide. Among nearly a hundred urine samples, 16 samples were found to contain plasmids with resistance to various antibiotics. The research focused on determining the resistant plasmid patterns in multi drug resistant Klebsiella pneumoniae and Proteus vulgaris isolated from urinary tract infection sites in hospital patients, as well as detecting and characterizing antimicrobial metabolites derived from marine sediments that exhibit potent activity against multidrug resistant pathogens.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Jiun-Nong Lin, Chung-Hsu Lai, Shang-Yi Lin, Ching-Chi Lee, Nan-Yao Lee, Po-Yu Liu, Chih-Hui Yang, Yi-Han Huang
Summary: This study evaluated the impact of intraspecific diversity among the multiple copies of the 16S rRNA gene on the accuracy of species identification in the genus Elizabethkingia. The results suggest that 16S rRNA gene sequencing should not be considered the gold standard for the accurate identification of Elizabethkingia species.
MICROBIOLOGY SPECTRUM
(2022)
Article
Medical Laboratory Technology
Z. Demirbas, H. Selcuk Ozger, Serap Suzuk-Yildiz, Zekiye Bakkaloglu, Murat Dizbay
Summary: In this study, we evaluated the in vitro activities of plazomicin in carbapenem resistant Klebsiella pneumoniae (CR-Kp) isolates and examined the distribution of aminoglycoside resistance genes. The results showed that plazomicin exhibited enhanced efficacy against CR-Kp isolates compared to other aminoglycosides, making it an effective treatment option.
CLINICAL LABORATORY
(2022)
Article
Chemistry, Multidisciplinary
Qiao Jin, Qinghua Zhang, He Bai, Amanda Huon, Timothy Charlton, Shengru Chen, Shan Lin, Haitao Hong, Ting Cui, Can Wang, Haizhong Guo, Lin Gu, Tao Zhu, Michael R. Fitzsimmons, Kui-juan Jin, Shanmin Wang, Er-Jia Guo
Summary: By synthesizing single-crystalline and precisely controlled Fe3N thin films, this study demonstrates the maintenance of high-temperature ferromagnetism in thin Fe3N layers and reveals strong in-plane anisotropy and changing anomalous Hall resistivity. Additionally, a significant exchange bias is observed at the Fe3N and CrN interfaces. This work provides guidance for exploring functional nitride films and their potential applications.
ADVANCED MATERIALS
(2023)
Review
Astronomy & Astrophysics
Jin Qiao, Zhao Li, Tao Zhu, Ran Ji, Guoliang Li, Wen Zhao
Summary: The examination of parity symmetry in gravitational interactions has gained attention, and theories of parity-violating gravity have been proposed. This review summarizes the progress of these theories and focuses on their observable effects in gravitational waves (GWs), particularly the difference between polarization modes. It explores the implications of these theories for GWs generated by compact binary coalescences and primordial GWs. The current and potential constraints on the parity-violating energy scales are presented using current and future GW observations.
FRONTIERS IN ASTRONOMY AND SPACE SCIENCES
(2023)
Review
Education & Educational Research
Hongyang Chen, Zining Wang, Lan Zheng, Jiumei Ciren, Weiyi Zhang, Tao Zhu
Summary: Anesthesiology education in Tibet lags behind, and clinical anesthesia needs improvement. Improving the overall quality of anesthesiology teachers in Tibet is advantageous for the development of local anesthesiology. Enhancing the teaching ability of anesthesiologists not only promotes the independent learning capability of young doctors, but also establishes a strong foundation for the smooth progress of clinical work.
FRONTIERS IN EDUCATION
(2023)
Article
Energy & Fuels
Mengye Jin, Tao Zhu, Shouheng Li, LiFeng Wang, Yusheng Liu, Yan Yu, Yiwei Han
Summary: In this study, CeMnOx-ZF catalysts with different metal loadings were prepared from coal fly ash (CFA) and their catalytic oxidation effects on toluene were investigated. The synergy between Ce and Mn metals in CeMnOx-ZF catalysts was studied. The optimized conditions for highest activity of CFA were determined, and the 20 wt.% CeMnOx-ZF catalyst exhibited a high toluene removal efficiency of up to 99.6% under optimized reaction conditions. The catalyst also showed high stability even after a 100-hour cycle test. This study provides a simple method for CFA activation and proposes a feasible strategy for designing high-efficiency catalysts for VOC degradation.
Article
Optics
Ligang Huang, Shunli Liu, Chaoze Zhang, Yanxiang Zhao, Laiyang Dang, Lei Gao, Wei Huang, Guolu Yin, Tao Zhu
Summary: This article proposes and demonstrates an acousto-optic reconfigurable filter (AORF) based on vector mode fusion in dispersion-compensating fiber (DCF). The resonance peaks of different vector modes in the same scalar mode group can be effectively fused into a single peak, allowing for arbitrary reconfiguration of the filter. In the experiment, the AORF's bandwidth can be electrically tuned from 5 nm to 18 nm by superimposing different driving frequencies. The AORF also demonstrates multi-wavelength filtering and the ability to reconfigure between bandpass and band-rejection modes by setting the combination of driving frequencies.
Article
Chemistry, Analytical
Guolu Yin, Zhaohao Zhu, Min Liu, Yu Wang, Kaijun Liu, Kuanglu Yu, Tao Zhu
Summary: We proposed an optical frequency domain reflectometry (OFDR) based on a multilayer perceptron (MLP) to train and grasp the fingerprint features of Rayleigh scattering spectrum in the optical fiber. The method achieves a larger measurement range, better measurement accuracy, and is less time-consuming compared to the traditional cross-correlation algorithm. This is the first time that machine learning has been introduced into an OFDR system, which brings new knowledge and optimization to the system.
Article
Medicine, General & Internal
Yusi Hua, Qiyuan Huang, Guo Chen, Tao Zhu
Summary: This study aims to investigate the effect of ultrasound-guided anterior superior laryngeal nerve block on awake videolaryngoscopes-assisted endotracheal intubation in adult elective surgery patients, and compare the effectiveness and safety to traditional ultrasound-guided posterior superior laryngeal nerve block.
Article
Immunology
Yidan Tang, Weizhi Liu, Weishuang Kong, Shuangyi Zhang, Tao Zhu
Summary: This study used a two-sample Mendelian randomization method to investigate the causal relationship between chronic pain and autoimmune diseases (AIDs). The results showed a causal relationship between multisite chronic pain and multiple sclerosis (MS) and rheumatoid arthritis (RA).
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Public, Environmental & Occupational Health
Yingyang Li, Wenjing Yu, Lamei Li, Qianqian Yao, Kexin Jiang, Tao Zhu, Enshe Jiang
Summary: Cancers have become the leading cause of death in China, greatly impacting the health and lives of residents. Oncology nursing focuses on cancer education, prevention, detection, and palliative care. While China has made progress in developing oncology nursing, there are still challenges that need to be addressed to ensure access to cancer care for more individuals.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Transportation Science & Technology
Jingke Zhang, Tao Zhu, Bing Yang, Xiaorui Wang, Shoune Xiao, Guangwu Yang, Yanwen Liu, Quanwei Che
Summary: Rail vehicles generate enormous longitudinal impact loads during collisions, and if there is a mismatch between the compressive strength of the intermediate coupler and the structural strength of the car body, the risk of car body damage and train derailment increases. In this study, a four-stage rigid-flexible coupling finite element model of the coupler is established, taking into account the coupler buckling load. The influence of the coupler buckling load on the train's longitudinal-vertical-horizontal buckling behavior is investigated, and the mechanism of horizontal buckling instability in train collisions is revealed.
RAILWAY ENGINEERING SCIENCE
(2023)
Article
Chemistry, Physical
Qi Zhou, Yu-Xuan Peng, Ping Wang, Zhong-Ying Jiang, Xin-Jun Zhao, Tao Zhu
Summary: The study aims to clarify the molecular mechanism underlying the asymmetric solution-induced membrane shape transition in lipid bilayers. Using giant unilamellar vesicles (GUVs) as model membranes, the interaction of fully hydrated lipid membranes with two distinct sugar solutions was investigated. The experimental observations revealed that asymmetric sugar solutions induce spontaneous curvature in the phospholipid bilayer, resulting in the formation of outward(/inward) budding vesicles over a wide range of parameters.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Automation & Control Systems
Minghui Chen, Ming Cai, Yadong Gong, Tao Zhu, Qiang Gong, Yu Liu
Summary: This study presents a novel three-dimensional simulation model for Abaqus multi-grit grinding developed using Python's secondary capabilities. The model uses a characteristic distribution pattern to arrange 48-sided abrasive grains on a cylinder, simulating a 200-grit micro-abrasive rod. Through considering various parameters, such as spindle speeds, grinding depths, and feed rates, the model explores the impact of multi-grit on the surface profiles of chips and workpieces for the nickel-based single-crystal Superalloy DD5. The findings from the multi-grain simulation provide valuable insights into the dynamics of multi-grit grinding processes and optimization strategies.
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2023)
Article
Immunology
Changliang Liu, Jiahui Wu, Ming Li, Rui Gao, Xueying Zhang, Shixin Ye-Lehmann, Jiangning Song, Tao Zhu, Chan Chen
Summary: This study found that elevated Smad7 in the hippocampal CA1 region can enhance neuroinflammation and apoptosis, leading to learning and memory impairment after surgery. Therefore, Smad7 may be a potential therapeutic target for the treatment of memory impairment after anesthesia surgery.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Optics
Wang Yu, Xiao Di, Niu Yangyang, Yang Jie, Yang Pengxi, Zhu Zhaohao, Yin Guolu, Zhu Tao
Summary: Objective Fiber optic hydrophones are underwater acoustic sensors that convert sound signals into optical signals for data processing. They have high sensitivity, resistance to electromagnetic radiation, and are suitable for harsh conditions. With the increase in maritime activities, research on hydrophone arrays has become important. However, the probe technology is crucial for the stability and performance of hydrophones. This study proposes a high-precision measurement method based on optical frequency domain reflectometry to monitor the deformation of hydrophone probes.
ACTA OPTICA SINICA
(2023)
Article
Optics
Laiyang Dang, Ligang Huang, Leilei Shi, Fuhui Li, Guolu Yin, Lei Gao, Tianyi Lan, Yujia Li, Lidan Jiang, Tao Zhu
Summary: This study proposes a new method to deeply purify laser spectrum with compact size under normal conditions, by effectively suppressing the spontaneous radiation of the laser cavity through weak external distributed perturbation. By injecting a feedback signal with continuous spatio-temporal phase transition controlled by a distributed feedback structure into the main cavity, the coupling rate from the spontaneous radiation to the stimulated emission can be deeply suppressed, resulting in an extremely purified laser spectrum. Eventually, an ultra-narrow linewidth on-chip laser system with excellent performance is successfully obtained under normal conditions.
OPTO-ELECTRONIC ADVANCES
(2023)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)