Article
Immunology
Marwa Abd El-Hamid, Alaa H. Sewid, Mohamed Samir, Wael A. H. Hegazy, Mosa M. Bahnass, Rasha A. Mosbah, Doaa M. Ghaith, Eman Khalifa, Hazem Ramadan, Walaa A. Alshareef, Hanan M. Alshareef, Mohammed M. Ghoneim, Mohammad M. Al-Sanea, Mahmoud M. Bendary
Summary: This study aimed to determine the diversity of the predominant MRSA clone ST239-MRSA in Port-said and El-Sharkia Governorates, Egypt, and to explore the correlation between resistance patterns, toxin genes, and the genetic background. The analysis revealed a high degree of phenotypic and genotypic diversity among ST239-MRSA strains, indicating weak clonality and adaptability despite belonging to a single sequence type.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Marwa I. Abd El-Hamid, Reham M. El-Tarabili, Mosa M. Bahnass, Mohammed Abdulrahman Alshahrani, Ahmed Saif, Khairiah Mubarak Alwutayd, Fatmah Ahmed Safhi, Abdallah Tageldein Mansour, Noaf Abdullah N. Alblwi, Mohammed M. Ghoneim, Ayman Abo Elmaaty, Helal F. Al-harthi, Mahmoud M. Bendary
Summary: This study evaluated the multidrug resistance and multivirulence profiles of Staphylococcus aureus strains isolated from cows with mastitis. All isolates were found to be biofilm-producing methicillin-resistant S. aureus (MRSA) strains. The tested essential oils (EOs) demonstrated antimicrobial and antibiofilm activities that matched with the phenotypic evaluation and molecular docking studies. Synergistic interactions between the investigated EOs and methicillin or vancomycin were detected. Additionally, 13 isolates showed a change in antimicrobial resistance patterns to sensitive phenotypes after treatment with any of the investigated EOs. Combining the EOs with available antimicrobial drugs can prevent treatment failure of bovine mastitis caused by resistant S. aureus.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Nelson E. Masota, Knut Ohlsen, Lorenz Meinel, Ulrike Holzgrabe
Summary: The ongoing threat of antimicrobial resistance and multidrug-resistant pathogens calls for increased efforts in the search for novel treatment options. This study synthesized a series of gluco-vanillin derivatives and found that compounds containing 2,4- and 3,5-dichlorophenylamino group coupled to a glucovanillin moiety exhibited optimum antibacterial activities. These derivatives showed moderate and broad-spectrum activities against reference and multidrug-resistant strains.
Article
Food Science & Technology
Candan Gungor, Mukaddes Barel, Adalet Dishan, H. Burak Disli, Kursat Koskeroglu, Nurhan Ertas Onmaz
Summary: The study investigated the prevalence of MSSA and MRSA in pastirma production chain, identifying strains with multidrug resistance and carrying enterotoxin genes. The low detection frequency of S. aureus strains in the analyzed samples could still pose a significant public health risk.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Infectious Diseases
Maria Tsalidou, Theodouli Stergiopoulou, Ioannis Bostanitis, Christina Nikaki, Kalypso Skoumpa, Theofani Koutsoukou, Paraskevi Papaioannidou
Summary: This retrospective study investigates antimicrobial susceptibility and multidrug resistance prevalence of clinical isolates in a regional hospital in Northern Greece over a period of six years. The study reveals a significant increase in multidrug-resistant bacterial strains, particularly in the intensive care unit.
Article
Microbiology
Carlos Ratia, Virginio Cepas, Raquel Soengas, Yolanda Navarro, Maria Velasco-de Andres, Maria Jose Iglesias, Francisco Lozano, Fernando Lopez-Ortiz, Sara M. Soto
Summary: The emergence and spread of infections caused by multidrug-resistant bacteria pose a threat to the effectiveness of current antibiotics. Gold-based drugs, specifically gold(III) complexes, have shown potential as antibacterial agents. In this study, the antibacterial activity of the gold(III) complex 2 was demonstrated, and a synergistic effect was observed when combined with colistin.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Mikael Kajova, Tamim Khawaja, Anu Kantele
Summary: ESBL-PE carriage was relatively common (12.5%) after hospitalization in European countries, while other MDROs proved less frequent (<5%). Antibiotic treatment and short time since hospitalization abroad increased the risk of MDRO colonization. Clear differences between countries and regions were revealed, with highest rates in the east and the south.
JOURNAL OF TRAVEL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Saraswathy Nagendran, Sakthivel Balasubramaniyan, Navabshan Irfan
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant tuberculosis (MDR-TB) are major causes of severe hospitalization and infection-related mortality in the general population. This study focuses on the development of novel sulfathiazole derivatives as potential drugs to combat MRSA and MDR-TB infections. Through virtual screening and molecular dynamics simulation, 10 promising compounds with potential anti-MRSA and anti-MDR-TB properties were identified and suggested as potential therapeutic leads.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Immunology
Sherief M. Abdel-Raheem, Marwa I. Abd El-Hamid, Doaa Ibrahim, Rania M. S. El-Malt, Waleed Rizk El-Ghareeb, Hesham A. Ismail, Saad Ibrahim Al-Sultan, Ahmed M. A. Meligy, Reham M. Eltarabili
Summary: This study investigated the antimicrobial resistance and virulence genes profiling of MRSA isolates and explored the antivirulence efficacy of trans-cinnamaldehyde, thymol, and carvacrol essential oils (EOs) against multi-virulent and MDR-MRSA isolates. The results showed superior in vitro antimicrobial and antivirulence activities of trans-cinnamaldehyde, thymol, and carvacrol against MRSA isolates. These essential oils inhibited the growth of multi-virulent and MDR-MRSA isolates and downregulated the transcription of examined virulence genes.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Carlos Ratia, Victoria Ballen, Yaiza Gabasa, Raquel G. Soengas, Maria Velasco-de Andres, Maria Jose Iglesias, Qing Cheng, Francisco Lozano, Elias S. J. Arner, Fernando Lopez-Ortiz, Sara M. Soto
Summary: A (C<^>S)-cyclometallated Au(III) dithiocarbamate complex was characterized as a potential drug candidate with stable properties, potent antibacterial and antibiofilm activity, and the ability to inhibit multidrug-resistant strains without producing resistant mutants. Mechanistic studies revealed its multimodal mechanism of action, including direct interaction with bacterial membranes and interference with energy metabolism and membrane stability pathways. The complex showed low cytotoxicity to mammalian cells and no acute in vivo toxicity in mice.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Leilei Zhang, Maha M. Ismail, Gabriele Rocchetti, Nesrin M. Fayek, Luigi Lucini, Fatema R. Saber
Summary: This study evaluated the efficacy of extracts from Aphanamixis polystachya, Toona ciliata, and Melia azedarach leaves against MRSA and cancer cells. The extracts of A. polystachya and M. azedarach were found to be rich in bioactive compounds with antimicrobial and anti-virulence properties, making them potential sources for cytotoxic and antibiofilm agents.
Article
Public, Environmental & Occupational Health
Heba Alhmidi, Jennifer L. Cadnum, Sreelatha Koganti, Annette L. Jencson, Brigid M. Wilson, Curtis J. Donskey
Summary: Limited information is available on the frequency of and risk factors for shedding of health care-associated pathogens in settings outside patient rooms. The study found that environmental shedding of MRSA occurs frequently during appointments outside hospital rooms or during outpatient clinic visits, and the presence of a wound with a positive culture for MRSA was associated with shedding of MRSA during appointments.
AMERICAN JOURNAL OF INFECTION CONTROL
(2021)
Article
Microbiology
Rasoul Mirzaei, Hadi Esmaeili Gouvarchin Ghaleh, Reza Ranjbar
Summary: The study demonstrates that melittin alone exhibits strong inhibitory and eradication effects on biofilms formed by multidrug-resistant pathogens. Furthermore, melittin shows synergistic effects when combined with antibiotics, resulting in decreased toxicity. Therefore, the combination of melittin and antibiotics has the potential to be a candidate for further evaluation in vivo for infections caused by multidrug-resistant pathogens.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
F. Hong, S. Salmon, X. Y. Ong, K. Liew, Y. Koh, A. Young, B. Ang, M. L. Foo, L. C. Lee, M. L. Ling, K. Marimuthu, S. Pada, B. F. Poh, K. C. Thoon, D. Fisher
Summary: In Singaporean acute healthcare facilities, while universal MRSA entry swabbing is practiced in accordance with national policy, there are significant variations in the response to positive and negative surveillance swabs, including the role of routine antiseptic bathing and MRSA decolonization. Most facilities view decolonization as 'bioburden reduction' rather than long-term clearance.
JOURNAL OF HOSPITAL INFECTION
(2021)
Article
Chemistry, Multidisciplinary
Sereen M. B. Bataineh, Yaser H. Tarazi, Wafa A. Ahmad
Summary: This study evaluated the antimicrobial activity of methanolic plant extracts against multidrug-resistant bacteria and confirmed their efficacy against MRSA in experimental animals. Ten out of sixty folk plants showed activities against MDR bacteria, with Camellia sinensis and Aloysia citrodora exhibiting the highest activities against MRSA and MSSA isolates. Further studies revealed low toxicity of these plant extracts against eukaryotic cells and their potential as alternative antibacterial agents.
APPLIED SCIENCES-BASEL
(2021)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)