Article
Immunology
Ebin Johny, Pathoori Bhaskar, Md Jahangir Alam, Indra Kuladhipati, Rupam Das, Ramu Adela
Summary: The study revealed increased platelet activation and platelet-immune cell aggregate formation in T2DM and T2DM_CAD groups compared to the control and CAD groups. Immunome profiling showed altered monocyte subpopulations and dendritic cell populations in T2DM, CAD, and T2DM_CAD groups. Additionally, significantly elevated levels of inflammatory cytokines/chemokines and decreased levels of certain immune factors were observed in the T2DM_CAD group compared to the control group.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Cell Biology
Gala Freixer, Khaoula Zekri-Nechar, Jose J. Zamorano-Leon, Carlos Hugo-Martinez, Nora Butta, Elena Monzon, Maria-Jose Recio, Manel Giner, Antonio Lopez-Farre
Summary: Exposure of megakaryocytes to low ASA concentration leads to higher apoptotic sensitivity in the newly generated platelet-like particles under stimulating conditions, possibly due to differences in mitochondrial functionality and NO formation.
Article
Medical Laboratory Technology
Gemma Chiva-Blanch, Esther Pena, Judit Cubedo, Maisa Garcia-Arguinzonis, Adriana Pane, Pedro A. Gil, Antonio Perez, Emilio Ortega, Teresa Padro, Lina Badimon
Summary: The study aimed to investigate the differences in platelets between diabetic patients and nondiabetic controls, revealing changes in platelet aggregation, cell migration, and cell homeostasis proteins. Platelets from diabetic subjects showed higher aggregation tendencies and higher levels of the stress-related protein complex HSPA8/Hsp90/CSK2a. The findings suggest that the complex HSPA8/Hsp90/CSK2a plays a role in diabetes-related platelet hyperreactivity and could be a potential target for future preventive and therapeutic strategies for platelet dysfunction in diabetes.
TRANSLATIONAL RESEARCH
(2021)
Article
Hematology
Fredy Delgado Lagos, Amro Elgheznawy, Anastasia Kyselova, Dagmar Meyer zu Heringdorf, Corina Ratiu, Voahanginirina Randriamboavonjy, Alexander W. Mann, Beate Fisslthaler, Mauro Siragusa, Ingrid Fleming
Summary: SMOC1 is identified as a novel thrombin-activating protein that is significantly upregulated in platelets from individuals with type 2 diabetes, contributing to platelet dysfunction in diabetes. Targeting SMOC1 or its interaction with thrombin may be a promising therapeutic approach for normalizing platelet function in diabetes.
Article
Cell Biology
Fionnuala Mone, Juhi K. Gupta, Marie M. Phelan, Shireen Meher, Lu Yung Lian, Ben Francis, Eunice Zhang, Cecilia Mulcahy, Ana Alfirevic, Fionnuala M. Mcauliffe, Kate Navaratnam
Summary: This multi-center prospective study recruited pregnant women taking low dose aspirin to develop reference intervals for a range of laboratory based platelet function tests, identify genomic variants influencing platelet response to aspirin, and evaluate the effectiveness of biochemical testing strategies. The study found that combining 11DTXB2 with adherence testing was an accurate strategy for detecting aspirin responsiveness in pregnant women, and no significant genetic variants were associated with aspirin response in pregnancy. Further research is needed to optimize dosing and benefits for specific subgroups.
Article
Endocrinology & Metabolism
Valentina Mancioppi, Erica Pozzi, Sara Zanetta, Anna Missineo, Silvia Savastio, Fabrizio Barbetti, Simona Mellone, Mara Giordano, Ivana Rabbone
Summary: Neonatal diabetes mellitus (NDM) is a rare genetic disease characterized by severe hyperglycemia. The disease can be temporary or permanent, and is often caused by abnormalities in specific genetic regions or mutations in certain genes. Insulin therapy can be switched to hypoglycemic sulfonylureas after the acute phase, but the timing of this switch can affect long-term complications.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Ravi Retnakaran, Chang Ye, Anthony J. Hanley, Philip W. Connelly, Mathew Sermer, Bernard Zinman, Jill K. Hamilton
Summary: Despite reducing birth weight and macrosomia, the treatment of GDM does not have analogous effects on infant adiposity across the 1st year of life.
Article
Biochemistry & Molecular Biology
Ivana A. Rukavina-Mikusic, Micaela Rey, Manuela Martinefski, Valeria Tripodi, Laura B. Valdez
Summary: This study aimed to investigate the temporal evolution of cardiac mitochondrial dysfunction in a type 1 diabetes model, finding that dysfunction occurred in the early stage of diabetic rats, similar to 7 days of hyperglycemia. Early subcellular signals of cardiac mitochondrial dysfunction include complex I, H2O2, and NO, with NO and H2O2 being located upstream de novo synthesis of mitochondria.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Jasmin Chendi Li, Anida Velagic, Cheng Xue Qin, Mandy Li, Chen Huei Leo, Barbara K. Kemp-Harper, Rebecca H. Ritchie, Owen L. Woodman
Summary: In diabetic rat carotid arteries, endothelial dysfunction impairs the sensitivity and maximal relaxation response to acetylcholine. Diabetes affects the contribution of nitric oxide, while HNO-mediated vasorelaxation remains intact.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Nutrition & Dietetics
Ichiro Kishimoto, Akio Ohashi
Summary: In middle aged males with overweight/obesity but without diabetes, snacking and physical inactivity serve as the major drivers of postprandial hyperglycemia independently of beta-cell function.
Article
Cardiac & Cardiovascular Systems
Helmut Raphael Lieder, Maria Tsoumani, Ioanna Andreadou, Karsten Schroer, Gerd Heusch, Petra Kleinbongard
Summary: The role of platelets during myocardial ischemia/reperfusion (I/R) is ambivalent. Remote ischemic conditioning (RIC) reduces myocardial I/R injury and platelet activation. This study found that platelets serve as carriers for RIC's cardioprotective signal and aspirin-sensitive mechanism plays a role in this process.
CARDIOVASCULAR DRUGS AND THERAPY
(2023)
Article
Cardiac & Cardiovascular Systems
Zhiyong Qi, Liang Hu, Jianjun Zhang, Wenlong Yang, Xin Liu, Daile Jia, Zhifeng Yao, Lin Chang, Guanxing Pan, Haoxuan Zhong, Xinping Luo, Kang Yao, Aijun Sun, Juying Qian, Zhongren Ding, Junbo Ge
Summary: PCSK9 in plasma directly enhances platelet activation and in vivo thrombosis by binding to platelet CD36 and activating downstream signaling pathways. PCSK9 inhibitors or aspirin can abolish the enhancing effects of PCSK9.
Article
Cardiac & Cardiovascular Systems
Ilaria Cavallari, Giuseppe Patti, Ernesto Maddaloni, Francesco Veneziano, Fabio Mangiacapra, Elisabetta Ricottini, Raffaella Buzzetti, Gian Paolo Ussia, Francesco Grigioni
Summary: This study investigated the role of low platelet reactivity (LPR) in the bleeding risk stratification of patients who underwent percutaneous coronary intervention (PCI) according to their diabetes mellitus (DM) status. The results showed that LPR provided significant incremental value for predicting bleeding, regardless of DM status. The coexistence of DM and LPR identified the subgroup with the highest bleeding risk.
AMERICAN JOURNAL OF CARDIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Hirotomo Nakahara, Tania Sarker, Christina L. Dean, Susana L. Skukalek, Roman M. Sniecinski, C. Michael Cawley, Jeannette Guarner, Alexander Duncan, Cheryl L. Maier
Summary: This study evaluated the agreement of four different testing platforms used to monitor antiplatelet effects. The results showed that LTA and VerifyNow had the highest agreement for P2Y12-I response, followed by moderate agreement between LTA and WBA. In terms of ASA response, LTA and AspirinWorks showed moderate agreement, while WBA showed the weakest agreement with both LTA and AspirinWorks.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Chemistry, Medicinal
Kutlwano R. R. Xulu, Tanya N. N. Augustine
Summary: There is a well-described association between cancer and a hypercoagulatory environment. Thrombotic complications not only pose a major mortality risk, but also significantly enhance tumor progression. The use of anticoagulant or antiplatelet drugs in cardiovascular disease may lessen thrombotic effects, but their impact on tumor progression needs further investigation. Published data suggests that interactions between drugs and targets within the tumor microenvironment may act as selective pressures, enhancing tumor survival and progression.
Letter
Infectious Diseases
Emanuela Falcinelli, Eleonora Petito, Cecilia Becattini, Edoardo De Robertis, Ugo Paliani, Manuela Sebastiano, Gaetano Vaudo, Giuseppe Guglielmini, Francesco Paciullo, Vittorio Cerotto, Marco Malvestiti, Fabio Gori, Loredana Bury, Teseo Lazzarini, Paolo Gresele
JOURNAL OF INFECTION
(2021)
Article
Cardiac & Cardiovascular Systems
Stefania Momi, Emanuela Falcinelli, Eleonora Petito, Giulia Ciarrocca Taranta, Alice Ossoli, Paolo Gresele
Summary: Platelets containing and releasing MMP-2 play a crucial role in the development and progression of atherosclerosis. Targeting platelet MMP-2 may provide a new approach for preventing atherosclerosis.
EUROPEAN HEART JOURNAL
(2022)
Article
Hematology
Loredana Bury, Emanuela Falcinelli, Anna Maria Mezzasoma, Giuseppe Guglielmini, Stefania Momi, Paolo Gresele
Summary: Platelet dysfunction in platelet-type von Willebrand disease is characterized by impaired aggregation, defective granule secretion, and reduced adhesion. This dysfunction is caused by abnormal inhibitory signaling triggered by the constitutive binding of von Willebrand factor to glycoprotein Ib alpha on platelets.
Article
Hematology
Giulio Purgatorio, Elisa Piselli, Giuseppe Guglielmini, Emanuela Falcinelli, Loredana Bury, Valeria Di Battista, Fabrizia Pellanera, Francesca Milano, Caterina Matteucci, Cristina Mecucci, Paolo Gresele
Summary: GATA2 deficiency can lead to thrombosis and defective angiogenesis, possibly by regulating the function of eNOS in endothelial cells. Atorvastatin may restore the eNOS expression and angiogenesis impairment caused by GATA2 deficiency by increasing the expression of the eNOS transcription factor AP-1/c-JUN.
Article
Hematology
Stefania Momi, Jessica Canino, Mauro Vismara, Luca Galgano, Emanuela Falcinelli, Giuseppe Guglielmini, Giulia Ciarrocca Taranta, Gianni Francesco Guidetti, Paolo Gresele, Mauro Torti, Ilaria Canobbio
Summary: The study elucidates the role of Pyk2 in venous thrombosis, showing that Pyk2 regulates thromboinflammatory responses of endothelial cells, leukocytes, and platelets, making it a potential target for the treatment of deep vein thrombosis.
Letter
Medicine, General & Internal
Emanuela Falcinelli, Giacomo Isernia, Giuseppe Guglielmini, Andrea Baccolo, Stefano Pasquino, Francesco Paciullo, Massimo Lenti, Paolo Gresele
INTERNAL AND EMERGENCY MEDICINE
(2022)
Article
Hematology
Loredana Bury, Emanuela Falcinelli, Haripriya Kuchi Bhotla, Anna Maria Mezzasoma, Giuseppe Guglielmini, Alexander Tischer, Laurie Moon-Tasson, Matthew Auton, Paolo Gresele
Summary: This study identified a novel GP1BA variant (p.Arg127Gln) that affects the structure and binding affinity of GPIb alpha protein for VWF. The findings suggest that GOF variants occurring outside the GPIb alpha C-terminal disulfide loop may be pathogenic in causing PT-VWD.
Article
Hematology
Eleonora Petito, Elisabetta Colonna, Emanuela Falcinelli, Anna Maria Mezzasoma, Enrica Cesari, Elisa Giglio, Tiziana Fiordi, Fabio Almerigogna, Alfredo Villa, Paolo Gresele
Summary: A study found that an immunological reaction involving platelets is not uncommon in individuals receiving anti-SARS-CoV-2 vaccination, especially after ChAdOx1 and Ad26.COV2.S, and that it associates with in vivo platelet and blood clotting activation.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Rheumatology
Vittorio Pengo, Teresa Del Ross, Marta Tonello, Laura Andreoli, Angela Tincani, Paolo Gresele, Elena Silvestri, Paolo Simioni, Elena Campello, Ariela Hoxha, Anna Falanga, Angelo Ghirarduzzi, Gentian Denas
Summary: This nationwide multicenter survey evaluated the course of COVID-19 and adverse events after vaccination in patients with triple-positive thrombotic APS. The results showed that these patients did not suffer from severe COVID-19 outcomes and COVID-19 vaccination was well tolerated. These findings can help reassure patients and physicians and reduce hesitancy in unvaccinated individuals.
Article
Hematology
Diego Mezzano, Paul Harrison, Andrew L. Frelinger, Andrew D. Mumford, Patrizia Noris, Marie Lordkipanidze, Paolo Gresele
Summary: This study aimed to provide expert consensus guidance on the use of platelet secretion assays (PSAs) for diagnosing inherited platelet function disorders (IPFD). Through a survey and evaluation of 26 experts from 10 different countries, the study identified some general agreements and specific assays that are appropriate for measuring platelet secretion. However, there is a lack of consensus on many other PSAs, indicating the need for further standardization and validation.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
G. Simonte, G. Guglielmini, E. Falcinelli, G. Isernia, A. M. Mezzasoma, P. Gresele, M. Lenti
Summary: This study aims to evaluate the association between high-on-treatment platelet reactivity (HTPR) in patients on aspirin plus clopidogrel therapy after carotid artery stenting (CAS) and subsequent major adverse cardiovascular events (MACE). The results demonstrate that HTPR has predictive value for MACE, with HTPR to three different assays being the most effective predictor.
THROMBOSIS RESEARCH
(2023)
Letter
Hematology
Andrea Baccolo, Emanuela Falcinelli, Anna Maria Mezzasoma, Giuseppe Guglielmini, Mariachiara Borghi, Loredana Bury, Paolo Gresele
THROMBOSIS RESEARCH
(2023)
Article
Hematology
Eleonora Petito, Laura Franco, Emanuela Falcinelli, Giuseppe Guglielmini, Chiara Conti, Gaetano Vaudo, Ugo Paliani, Cecilia Becattini, Antonella Mencacci, Francesca Tondi, Paolo Gresele
Summary: This study demonstrates that vaccination reduces platelet, neutrophil, and endothelial activation in COVID-19 patients, leading to a decrease in thrombotic events, intensive care unit admission, and death.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Hematology
Eleonora Petito, Paolo Gresele
Summary: Vaccine-induced immune thrombotic thrombocytopenia (VITT) is a rare but life-threatening complication of adenoviral vector vaccines, which was recognized around 2 years ago at the beginning of the anti-SARS-CoV-2 vaccination campaign. Despite the abandonment of VITT-provoking vaccines in most high-income countries, it is still important to study VITT due to the unvaccinated population in low/middle-income countries and the use of adenoviral vector platform for other vaccines. This review aims to provide an overview of VITT, including its clinical presentation, pathophysiology, diagnosis, management strategies, and future research needs.
THROMBOSIS AND HAEMOSTASIS
(2023)