Article
Multidisciplinary Sciences
Dipankar Ash, Varadarajan Sudhahar, Seock-Won Youn, Mustafa Nazir Okur, Archita Das, John P. O'Bryan, Maggie McMenamin, Yali Hou, Jack H. Kaplan, Tohru Fukai, Masuko Ushio-Fukai
Summary: This study reveals a previously unknown interaction between the Copper transporter ATP7A, autophagy, and VEGFR2 degradation in endothelial cells, highlighting the importance of ATP7A in regulating VEGFR2 signaling and angiogenesis. The findings suggest that ATP7A acts as a potential therapeutic target for ischemic cardiovascular diseases by promoting VEGFR2-mediated angiogenesis and neovascularization.
NATURE COMMUNICATIONS
(2021)
Article
Physiology
Christina Sul, Caitlin Lewis, Nathan Dee, Nana Burns, Kaori Oshima, Eric Schmidt, Christine Vohwinkel, Eva Nozik
Summary: Acute respiratory distress syndrome (ARDS) is a significant cause of morbidity and mortality in critically ill patients. This study investigates the protective effects of the R213G variant of extracellular superoxide dismutase (EC-SOD) in a methicillin-resistant S. aureus (MRSA) pneumonia model. The results suggest that the R213G EC-SOD variant preserves the integrity of the alveolar-capillary interface, attenuates proinflammatory cytokine levels, reduces neutrophil recruitment and neutrophil extracellular trap (NET) generation, and prevents extrapulmonary dissemination of bacteria.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Engineering, Biomedical
Motika Geethika, Namrata Singh, Sagar Kumar, Somanathapura K. Naveen Kumar, Govindasamy Mugesh
Summary: The conversion of nitric oxide (NO) into peroxynitrite (ONOO-) in a superoxide (O-2(.-))-rich environment is a major cause of reduced NO levels in vascular walls, leading to cardiovascular complications. Superoxide dismutase (SOD) mimetics that can selectively catalyze the dismutation of O-2(.-) in the presence of NO are challenging to design but have therapeutic relevance. In this study, the authors show that SOD mimetic cerium vanadate (CeVO4) nanozymes effectively regulate the bioavailability of NO and O-2(.-) even in the absence of cellular SOD enzyme, leading to improved endothelial function and potential applications in biological treatments.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Maxwell Mathias, Joann Taylor, Elizabeth Mendralla, Marta Perez
Summary: The study on bronchopulmonary dysplasia (BPD) in a mouse model found that SOD3 knockout (KO) mice exhibited better survival and less alveolar and microvascular remodeling under chronic hyperoxia exposure. Additionally, SOD3 KO mice showed increased total SOD activity and VEGF expression compared to wild-type mice.
Article
Pharmacology & Pharmacy
Yang Yang, Ming Zhao, Xi He, Qing Wu, Dong-Ling Li, Wei-Jin Zang
Summary: The disruption of gut microbes, BCAA catabolism, and vagal activity in diabetic cardiomyopathy mice were improved by pyridostigmine, leading to reduced cardiac damage. This study provides new insights for developing therapeutic strategies targeting gut microbes and BCAA metabolism to alleviate diabetes-induced cardiac injury.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hyehun Choi, Michael R. Miller, Hong-Ngan Nguyen, Victoria E. Surratt, Stephen R. Koch, Ryan J. Stark, Fred S. Lamb
Summary: TNF alpha activates NADPH oxidase 1, which produces extracellular superoxide anion. The secreted protein ecSOD converts superoxide anion to hydrogen peroxide, playing a role in TNF alpha signaling in vascular smooth muscle cells. Knockdown of ecSOD suppresses downstream TNF alpha signals and reduces expression of TNF alpha receptors.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Health Care Sciences & Services
Irina A. Mednova, Liudmila P. Smirnova, Alisa R. Vasilieva, Daria Kazantseva, Elena Epimakhova, Nina M. Krotenko, Arkadiy Semke, Svetlana A. Ivanova
Summary: This study demonstrates that patients with schizophrenia have SOD activity in their IgG, and the highest activity is observed in patients in therapeutic remission. The mechanism of SOD activity in IgG is similar to classical enzyme catalysis, with a higher affinity for the substrate than classical SOD enzyme.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Environmental Sciences
Paolo Pastorino, Elisabetta Pizzul, Damia Barcelo, Maria Cesarina Abete, Gabriele Magara, Paola Brizio, Rosa Avolio, Marco Bertoli, Alessandro Dondo, Marino Prearo, Antonia Concetta Elia
Summary: This study investigated the oxidative stress ecology in Danube barbel from the Barbucina creek, analyzing the levels of antioxidant biomarkers and trace elements in the fish. Results indicated a significant association between certain trace elements and enzyme activity, suggesting their role as stressors in the aquatic environment. The study provides insights into the biochemical defensive response of fish to environmental disturbances, advancing our understanding of the effects of environmental factors on oxidative stress in aquatic ecosystems.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Peripheral Vascular Disease
Zhanhui Wang, Fupeng Yang, Zhiqing He, Chun Liang
Summary: This study demonstrates that circadian rhythm disorders can induce the development of coronary microvascular dysfunction (CMD) through the up-regulation of NETs. Inhibiting NETs production can improve coronary microcirculatory function and endothelium-dependent diastolic function.
MICROVASCULAR RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Alessia Parascandolo, Mikko O. Laukkanen
Summary: Superoxide dismutase (SOD) family isoenzymes, including SOD1, SOD2, and SOD3, synthesize hydrogen peroxide (H2O2) to regulate signal transduction. The cellular response to SOD3 is dose-dependent, with high concentrations reducing cell survival.
Review
Biochemistry & Molecular Biology
Brianne R. O'Leary, Rory S. Carroll, Garett J. Steers, Jennifer Hrabe, Frederick E. Domann, Joseph J. Cullen
Summary: Reactive oxygen species are essential components in cell signaling and immune responses, but an imbalance can lead to oxidative stress. Cancer cells show increased oxidative stress and decreased expression of extracellular superoxide dismutase, an antioxidant enzyme that plays a role in maintaining redox balance and protecting against oxidative damage. Understanding the role of EcSOD in cancer could have potential implications for detection, prognosis, and treatment of malignancies.
Article
Food Science & Technology
Md. Khalid Juhani Rafi, Md. Atiar Rahman, Tanvir Ahmed Siddique, Farhana Yesmin Bithy, Sumaiya Akter, Rasheda Aktar, Fatema Yasmin Nisa, Md. Asif Nadim Khan, Md. Altaf Hossain, Srabonti Saha, A. M. Abu Ahmed
Summary: This study prepared bioactive protein hydrolysates from the freshwater snail Paludomas conica and investigated their antioxidant and antidiabetic effects. The results showed that the hydrolysates had potential in reducing diabetic complications by regulating the gene expression of antioxidant and glycolytic enzymes.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Medicine, General & Internal
Haiqing Li, Wei Hong, Zixiong Zeng, Shan Gong, Fan Wu, Zihui Wang, Heshen Tian, Juan Cheng, Ruiting Sun, Mi Gao, Chunxiao Liang, Weitao Cao, Guoping Hu, Yuqun Li, Liping Wei, Yumin Zhou, Pixin Ran
Summary: The study investigated the association between serum ecSOD activity and 1-year all-cause mortality in acute exacerbations of COPD (AECOPD) patients. The findings suggest that lower ecSOD activity is a strong independent predictor of 1-year all-cause mortality in AECOPD patients.
FRONTIERS IN MEDICINE
(2022)
Article
Neurosciences
Mami Yamada, Mitsuharu Okutsu
Summary: The study demonstrates that doxorubicin triggers severe muscle atrophy through oxidative stress and decreased expression of the antioxidant enzyme EcSOD. However, supplementation with EcSOD recombinant protein can inhibit oxidative stress and muscle atrophy. Transient treatment with interleukin-1 beta (IL-1 beta) can increase EcSOD expression and attenuate doxorubicin-induced muscle atrophy.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Review
Cell Biology
Xiangning Cui, Yan Wang, Han Liu, Mengjun Shi, Jingwu Wang, Yifei Wang
Summary: This article summarizes the effects of defective copper homeostasis on diabetic cardiomyopathy and discusses potential underlying molecular mechanisms.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Hematology
Varadarajan Sudhahar, Mustafa Nazir Okur, Zsolt Bagi, John P. O'Bryan, Nissim Hay, Ayako Makino, Vijay S. Patel, Shane A. Phillips, David Stepp, Masuko Ushio-Fukai, Tohru Fukai
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2018)
Article
Cell Biology
Young-Mee Kim, Seock-Won Youn, Varadarajan Sudhahar, Archita Das, Reyhaan Chandhri, Henar Cuervo Grajal, Junghun Kweon, Silvia Leanhart, Lianying He, Peter T. Toth, Jan Kitajewski, Jalees Rehman, Yisang Yoon, Jaehyung Cho, Tohru Fukai, Masuko Ushio-Fukai
Article
Cell Biology
Takashi Ashino, Takashi Kohno, Varadarajan Sudhahar, Dipankar Ash, Masuko Ushio-Fukai, Tohru Fukai
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2018)
Article
Multidisciplinary Sciences
Qiuhua Yang, Jiean Xu, Qian Ma, Zhiping Liu, Varadarajan Sudhahar, Yapeng Cao, Lina Wang, Xianqiu Zeng, Yaqi Zhou, Min Zhang, Yiming Xu, Yong Wang, Neal L. Weintraub, Chunxiang Zhang, Tohru Fukai, Chaodong Wu, Lei Huang, Zhen Han, Tao Wang, David J. Fulton, Mei Hong, Yuqing Huo
NATURE COMMUNICATIONS
(2018)
Article
Cell Biology
Archita Das, Varadarajan Sudhahar, Masuko Ushio-Fukai, Tohru Fukai
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2019)
Article
Hematology
Varadarajan Sudhahar, Archita Das, Tetsuo Horimatsu, Dipankar Ash, Silvia Leanhart, Olga Antipova, Stefan Vogt, Bhupesh Singla, Gabor Csanyi, Joseph White, Jack H. Kaplan, David Fulton, Neal L. Weintraub, Ha Won Kim, Masuko Ushio-Fukai, Tohru Fukai
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2019)
Article
Cell Biology
Varadarajan Sudhahar, Mustafa Nazir Okur, John P. O'Bryan, Richard D. Minshall, David Fulton, Masuko Ushio-Fukai, Tohru Fukai
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2020)
Article
Multidisciplinary Sciences
Dipankar Ash, Varadarajan Sudhahar, Seock-Won Youn, Mustafa Nazir Okur, Archita Das, John P. O'Bryan, Maggie McMenamin, Yali Hou, Jack H. Kaplan, Tohru Fukai, Masuko Ushio-Fukai
Summary: This study reveals a previously unknown interaction between the Copper transporter ATP7A, autophagy, and VEGFR2 degradation in endothelial cells, highlighting the importance of ATP7A in regulating VEGFR2 signaling and angiogenesis. The findings suggest that ATP7A acts as a potential therapeutic target for ischemic cardiovascular diseases by promoting VEGFR2-mediated angiogenesis and neovascularization.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Kareem Abdelsaid, Varadarajan Sudhahar, Ryan A. Harris, Archita Das, Seock-Won Youn, Yutao Liu, Maggie McMenamin, Yali Hou, David Fulton, Mark W. Hamrick, Yaoliang Tang, Tohru Fukai, Masuko Ushio-Fukai
Summary: Exercise can improve the angiogenic potential of circulating exosomes in patients with type 2 diabetes, and the presence of extracellular superoxide dismutase (SOD3) plays a crucial role in this process. SOD3 increases ATP7A in exosomes, leading to enhanced angiogenesis. Exosomes overexpressing SOD3 show promising results in promoting angiogenesis and wound repair.
Article
Cell Biology
Archita Das, Dipankar Ash, Abdelrahman Y. Fouda, Varadarajan Sudhahar, Young-Mee Kim, Yali Hou, Farlyn Z. Hudson, Brian K. Stansfield, Ruth B. Caldwell, Malgorzata McMenamin, Rodney Littlejohn, Huabo Su, Maureen R. Regan, Bradley J. Merrill, Leslie B. Poole, Jack H. Kaplan, Tohru Fukai, Masuko Ushio-Fukai
Summary: This study uncovers an important role of the copper transporter CTR1 as a redox sensor in endothelial cells, promoting VEGFR2 signaling and angiogenesis. CTR1 undergoes modifications after redox stress, leading to the formation of CTR1-VEGFR2 complex and enhancing VEGFR2 signaling and angiogenesis.
NATURE CELL BIOLOGY
(2022)
Article
Peripheral Vascular Disease
Sheela Nagarkoti, Young-Mee Kim, Dipankar Ash, Archita Das, Eric Vitriol, Tracy-Ann Read, Seock-Won Youn, Varadarajan Sudhahar, Malgorzata McMenamin, Yali Hou, Harriet Boatwright, Ruth Caldwell, David W. Essex, Jaehyung Cho, Tohru Fukai, Masuko Ushio-Fukai
Summary: PDIA1 senses the H2O2 signal induced by VEGF to promote VEGFR2 signaling and angiogenesis in endothelial cells, thereby enhancing postnatal angiogenesis.
Article
Cell Biology
Varadarajan Sudhahar, Yang Shi, Jack H. Kaplan, Masuko Ushio-Fukai, Tohru Fukai
Summary: The study found that deficiency of Atox1 reduces atherosclerotic lesion formation, while overexpression of nuclear-targeted Atox1 increases monocyte adhesion and reactive oxygen species production. Genome-wide mapping analysis revealed that Atox1 regulates various inflammatory and angiogenic genes in endothelial cells.
Article
Cardiac & Cardiovascular Systems
Abdelrahman A. Zaied, Masuko Ushio-Fukai, Tohru Fukai, Anita Kovacs-Kasa, Suhib Alhusban, Varadarajan Sudhahar, Vijay C. C. Ganta, Brian H. H. Annex
Summary: In endothelial cells, PFKFB3 plays a crucial role in regulating glycolysis, which is the main pathway for ATP generation. Both VEGF(165)a and microRNA-93 promote angiogenesis. In peripheral artery disease models, microRNA-93 treatment improves perfusion recovery, while VEGF(165)a treatment does not. This suggests that activating the glycolysis pathway to enhance angiogenesis may have therapeutic advantages.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Cardiac & Cardiovascular Systems
Austin T. Robinson, Ibra S. Fancher, Varadarajan Sudhahar, Jing Tan Bian, Marc D. Cook, Abeer M. Mahmoud, Mohamed M. Ali, Masuko Ushio-Fukai, Michael D. Brown, Tohru Fukai, Shane A. Phillips
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Seock-Won Youn, Yang Li, Young-Mee Kim, Varadarajan Sudhahar, Kareem Abdelsaid, Ha Won Kim, Yutao Liu, David J. R. Fulton, Muhammad Ashraf, Yaoliang Tang, Tohru Fukai, Masuko Ushio-Fukai