Article
Biochemistry & Molecular Biology
Sung-Wook Nam, Jeong-Pil Chae, Yong Hwan Kwon, Mi-Young Son, Jae-sung Bae, Mae-Ja Park
Summary: Xenopus laevis is a suitable animal model for toxicology research due to its advantages in embryogenesis. A convenient embryo housing device is essential for handling a large number of embryos simultaneously in toxicological studies. The 3D fabrication of single-egg trapping devices can be used to culture Xenopus embryos for efficient assessment of malformation caused by toxicants.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Neurosciences
Tamira Elul, John Lim, Kenneth Hanton, Austin Lui, Kenton Jones, George Chen, Celeste Chong, Sophia Dao, Radhika Rawat
Summary: This study investigates the effects of CB1R signaling on growth cone filopodia and axonal projections of retinal ganglion cells (RGCs) in developing neurons. The results suggest that CB1R agonist and inverse agonist have opposite effects on growth cone filopodia, and both destabilization and stabilization of growth cone filopodia are required for RGC axonal differentiation in the optic tract. Furthermore, CB1R regulates growth cone filopodia and axon dispersion of RGCs by modulating adhesive and actin regulatory functions.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2022)
Article
Neurosciences
Valentina Saccomanno, Heather Love, Amy Sylvester, Wen-Chang Li
Summary: The study showed that the lateral line system plays a crucial role in Xenopus tadpole motor responses to suction stimulation, initiating swimming reliably. Additionally, the afferent and efferent activities of the tadpole anterior lateral line nerve were analyzed, with sensory interneurons located using calcium imaging techniques.
JOURNAL OF NEUROPHYSIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Feifei Wang, Fuqiang Ren, Zhuoran Ma, Liangqiong Qu, Ronan Gourgues, Chun Xu, Ani Baghdasaryan, Jiachen Li, Iman Esmaeil Zadeh, Johannes W. N. Los, Andreas Fognini, Jessie Qin-Dregely, Hongjie Dai
Summary: By studying light scattering and utilizing new materials technology, the imaging depth of high-resolution optical microscopy in live mammals can be improved. This study demonstrates single-photon excitation fluorescence imaging in the NIR-IIc range, achieving the longest single-photon excitation and emission wavelengths to date for in vivo imaging of mice.
NATURE NANOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katrin Pratsch, Chie Unemura, Mana Ito, Stefan F. Lichtenthaler, Naotaka Horiguchi, Jochen Herms
Summary: This study investigated the effect of highly selective BACE1 inhibitors on dendritic spine dynamics in the somatosensory cortex of mice. The results showed that if soluble Sez6 (sSez6) levels drop too much, highly selective BACE1 inhibitors can alter dendritic spine density similar to non-selective inhibitors. Therefore, carefully adjusting the dosing to achieve an appropriate amount of Sez6 cleavage may be reasonable.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kirill S. Tenkov, Mikhail V. Dubinin, Alexander A. Vedernikov, Yuliya A. Chelyadnikova, Konstantin N. Belosludtsev
Summary: The present study investigates the in vivo effect of triclosan on frogs and reveals a dose-dependent impact on their survival. Triclosan damages the liver tissue, reduces the osmotic resistance of red blood cells, and suppresses the respiration rate and calcium retention capacity of frog liver mitochondria. These findings highlight the significance of triclosan on aquatic organisms.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Julian Schwarting, Kathrin Nehrkorn, Hanhan Liu, Nikolaus Plesnila, Nicole Angela Terpolilli
Summary: The study used in vivo two-photon microscopy to investigate the mechanism of pial microvasospasms (MVSs) in inducing cerebral ischemia after subarachnoid hemorrhage (SAH). Results showed that perivascular blood is associated with significant reductions in cortical perfusion, suggesting MVSs as a key mechanism in triggering cerebral ischemia after SAH. Further research on the mechanisms of pial vasospasm could lead to novel therapeutic options for SAH patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Oncology
Shrikant B. Mali
Summary: Intravital microscopy and optical coherency tomography are powerful imaging tools that have revolutionized cancer diagnosis and therapies. They allow for real-time observation of biological processes in vivo, providing insights into tumor behaviors. Recent developments in techniques for observing deep tissues have improved the detection of subcellular events and made these technologies indispensable in basic sciences and drug development.
Review
Neurosciences
Peter William Hogg, Patrick Coleman, Tristan Dellazizzo Toth, Kurt Haas
Summary: Recent technological advancements have enabled direct studies of neuronal morphogenesis, allowing for detailed quantification of structural changes over time. Dynamic morphometrics methods have provided new insights into growth patterns, molecular mechanisms, and the influence of external factors on neural development, particularly in vertebrate systems.
TRENDS IN NEUROSCIENCES
(2022)
Article
Biochemical Research Methods
Omkar D. Supekar, Andrew Sias, Sean R. Hansen, Gabriel Martinez, Graham C. Peet, Xiaoyu Peng, Victor M. Bright, Ethan G. Hughes, Diego Restrepo, Douglas P. Shepherd, Cristing G. Welle, Juliet T. Gopinath, Emily A. Gibson
Summary: This paper presents a high-resolution miniature, light-weight fluorescence microscope with electrowetting lens and onboard CMOS, capable of high-resolution volumetric imaging and structured illumination while rejecting out-of-focus and scattered light.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemical Research Methods
S. H. A. U. N. A. ENGELMANN, A. N. N. I. E. ZHOU, A. H. M. E. D. M. HASSAN, M. I. C. H. A. E. L. R. WILLIAMSON, J. E. R. E. M. Y. W. JARRETT, E. V. A. N. P. PERILLO, A. L. A. N. K. R. I. T. TOMAR, D. A. V. I. D. J. SPENCE, T. H. E. R. E. S. A. A. JONES, A. N. D. R. E. W. K. DUNN
Summary: This article introduces a fiber amplifier and a diamond Raman laser as excitation sources for two-photon excitation of red-shifted fluorophores. These sources output high powers at valuable wavelengths and are simple to construct and cost-efficient.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Chemistry, Multidisciplinary
Dongyu Li, Hequn Zhang, Lina L. Streich, Yalun Wang, Ping Lu, Ling Wang, Robert Prevedel, Jun Qian
Summary: The use of organic AIE nanoparticles with high absorption cross-section has enabled ultra-deep NIR-IIa excited three-photon imaging in the in vivo mouse brain.
MATERIALS CHEMISTRY FRONTIERS
(2021)
Article
Cell Biology
Sumika Kato, Takeo Kubo, Taro Fukazawa
Summary: In this study, tissue stem cells in regenerating Xenopus laevis tadpole tails were successfully enriched using the SP method and single-cell RNA sequencing. This finding is important for understanding the behavioral dynamics of tissue stem cells during tail regeneration.
DEVELOPMENT GROWTH & DIFFERENTIATION
(2022)
Article
Neurosciences
Lila D. Faulhaber, Olivia D'Costa, Andy Y. Shih, Juliane Gust
Summary: The aim of this study was to optimize the dosing and two-photon imaging parameters for leukocyte labeling in healthy mice and a venous microstroke model. The results showed that intravenous injection of 0.4mg/kg of anti-CD45.2 antibody was sufficient to label 95% of circulating leukocytes, and there was no depletion of circulating leukocytes after 24 hours at the tested dosages. The study demonstrated that the anti-CD45.2 mAb is a robust reagent for labeling leukocytes during in vivo two-photon microscopy.
Article
Cell Biology
Huan Xiong, Fei Tang, Yujie Guo, Ruxiang Xu, Peng Lei
Summary: Neural circuits are crucial for healthy brain function and disruptions can lead to diseases. Advanced microscopic techniques, such as two-photon laser scanning microscopy, have been used to study neural circuit changes during diseases. This review focuses on the dynamic rewiring of dendritic spines and modulation of calcium transients in the intact living brain, discussing their potential roles in neurological disorders like Alzheimer's disease, stroke, and epilepsy. Understanding these changes is important for future clinical interventions.
AGEING RESEARCH REVIEWS
(2023)
Review
Neurosciences
Janaina Brusco, Kurt Haas
JOURNAL OF PHYSIOLOGY-LONDON
(2015)
Article
Biochemistry & Molecular Biology
Simon Xuan Chen, Angus Cherry, Parisa Karimi Tari, Kaspar Podgorski, Yue Kay Kali Kwong, Kurt Haas
Article
Biochemical Research Methods
Kaspar Podgorski, Kurt Haas
JOURNAL OF BIOPHOTONICS
(2013)
Article
Neurosciences
Xue Feng Liu, Parisa Karimi Tari, Kurt Haas
JOURNAL OF NEUROSCIENCE
(2009)
Article
Biochemical Research Methods
Derek Dunfield, Kurt Haas
Article
Neurosciences
Simon Xuan Chen, Parisa Karimi Tari, Kevin She, Kurt Haas
Article
Multidisciplinary Sciences
Kaspar Podgorski, Ewald Terpetschnig, Oleksii P. Klochko, Olena M. Obukhova, Kurt Haas
Article
Biochemistry & Molecular Biology
Kaspar Podgorski, Derek Dunfield, Kurt Haas
Article
Multidisciplinary Sciences
Troy A. McDiarmid, Manuel Belmadani, Joseph Liang, Fabian Meili, Eleanor A. Mathews, Gregory P. Mullen, Ardalan Hendi, Wan-Rong Wong, James B. Rand, Kota Mizumoto, Kurt Haas, Paul Pavlidis, Catharine H. Rankin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Oncology
Jesse T. Chao, Rocio Hollman, Warren M. Meyers, Fabian Meili, Kenneth A. Matreyek, Pamela Dean, Douglas M. Fowler, Kurt Haas, Calvin D. Roskelley, Christopher J. R. Loewen
Article
Cell Biology
Barry P. Young, Kathryn L. Post, Jesse T. Chao, Fabian Meili, Kurt Haas, Christopher Loewen
DISEASE MODELS & MECHANISMS
(2020)
Article
Multidisciplinary Sciences
Kathryn L. Post, Manuel Belmadani, Payel Ganguly, Fabian Meili, Riki Dingwall, Troy A. McDiarmid, Warren M. Meyers, Caitlin Herrington, Barry P. Young, Daniel B. Callaghan, Sanja Rogic, Matthew Edwards, Ana Niciforovic, Alessandro Cau, Catharine H. Rankin, Timothy P. O'Connor, Shernaz X. Bamji, Christopher J. R. Loewen, Douglas W. Allan, Paul Pavlidis, Kurt Haas
NATURE COMMUNICATIONS
(2020)
Article
Neurosciences
Kelly D. R. Sakaki, Kaspar Podgorski, Tristan A. Dellazizzo Toth, Patrick Coleman, Kurt Haas
FRONTIERS IN NEURAL CIRCUITS
(2020)
Article
Genetics & Heredity
Fabian Meili, William J. Wei, Wun-Chey Sin, Warren M. Meyers, Iulia Dascalu, Daniel B. Callaghan, Sanja Rogic, Paul Pavlidis, Kurt Haas
Summary: SYNGAP1, a protein involved in regulating synaptic plasticity, has been linked to various neurological disorders. This study characterized 57 variants in multiple functional assays, revealing different impacts on protein function and stability. Variants within specific structural domains showed greater loss-of-function effects, while protein instability was a major factor for only two missense variants. High-content imaging identified subcellular localization differences that correlated with altered catalytic activity, indicating diverse molecular mechanisms underlying variant dysfunction. This multidimensional dataset provides insights for predicting the pathogenicity of SYNGAP1 variants.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)