Review
Neurosciences
Luca Pancotti, Lisa Topolnik
Summary: This article reviews the evidence regarding the modulatory effects of acetylcholine (ACh) on dendritic computations in three subtypes of hippocampal interneurons: fast-spiking parvalbumin-positive (PV+) cells, somatostatin-expressing (SOM+) oriens lacunosum moleculare cells, and vasoactive intestinal polypeptide-expressing (VIP+) interneuron-selective interneurons. The distribution and actions of cholinergic receptors on these interneurons are discussed, along with the potential modulation of dendritic Ca2+ signaling and neuronal activity. The article suggests that cholinergic modulation can shape dendritic integration and plasticity in interneurons in a cell type-specific manner, which is important for understanding their contribution to large-scale network activity.
Article
Cell Biology
Samet Kocaturk, Elif Beyza Guven, Fulva Shah, James M. Tepper, Maxime Assous
Summary: Cholinergic interneurons play crucial roles in striatal circuits and functions. The activation of acetylcholine signal via muscarinic receptors is well studied, but recent data suggest that nicotinic receptors located on GABAergic interneurons are equally important. Stimulation of cholinergic interneurons activates these receptors and leads to inhibitory effects on striatal projection neurons.
Article
Neurosciences
Szi-Chieh Yu, Jana F. Liewald, Jiajie Shao, Wagner Steuer Costa, Alexander Gottschalk
Summary: The release of neuropeptides is crucial for neuromodulation, and our study demonstrates that synapsin SNN-1 is necessary for cAMP-dependent neuropeptide release in Caenorhabditis elegans. Through capturing dense core vesicles (DCVs) at synapses, synapsin plays a key role in making them available for release. Mutations in synapsin result in altered neuropeptide release and behavior in the nematode, indicating the importance of synapsin in this process.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Neurosciences
Yuqi Ren, Yang Liu, Minmin Luo
Summary: The striatum is involved in various behaviors and its D1-type projection neurons are directly electrically coupled to cholinergic interneurons (ChIs), in addition to chemical transmission. Activation of D1 neurons promotes basal activity of ChIs through gap junctions, contributing to the maintenance of ChIs' tonically active firing patterns.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Ishika Kumar, Anju Paudyal, Anna Kadkova, Michelle Stewart, Jakob Balslev Sorensen, Julika Radecke
Summary: This article describes a straightforward method to establish a mature neuronal network on electron microscopy grids and visualize the formation of synaptic contacts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Claudia S. Bauer, Rebecca N. Cohen, Francesca Sironi, Matthew R. Livesey, Thomas H. Gillingwater, J. Robin Highley, Daniel J. Fillingham, Ian Coldicott, Emma F. Smith, Yolanda B. Gibson, Christopher P. Webster, Andrew J. Grierson, Caterina Bendotti, Kurt J. De Vos
Summary: A GGGGCC hexanucleotide repeat expansion in the C9ORF72 gene is the main genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia. This study shows that C9orf72 protein interacts with the synapsin family of synaptic vesicle proteins, and C9orf72 deficiency reduces the number of excitatory synapses and synapsin levels, leading to synaptic dysfunction.
ACTA NEUROPATHOLOGICA
(2022)
Article
Neurosciences
Amina Aboufares El Alaoui, Molly Jackson, Mara Fabri, Luisa de Vivo, Michele Bellesi
Summary: This study utilized serial block-face scanning electron microscopy to reconstruct and describe the intracellular organelles in perisynaptic astrocytic processes (PAPs) in two different mouse cortical regions. Five distinct organelles were identified within the PAPs, distributed among three categories. The majority of PAPs belonged to the leaflets category, with branches and branchlets displaying different organelle distributions. Mitochondria and ER cisternae were the most common organelles observed, with endosomes and phagosomes also frequently associated with the same PAP.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Neurosciences
Havard Ringsevjen, Daniel Lawer Egbenya, Malte Bieler, Svend Davanger, Suleman Hussain
Summary: This study provides detailed localization information of Arc protein at hippocampal synapses and reveals its presence in both presynaptic and postsynaptic cytoplasm associated with small vesicles. The protein is also found in presynaptic active zones and postsynaptic densities. Additionally, the study observes multivesicular body-like structures positive for Arc, suggesting a potential transsynaptic transmission of the protein in the brain.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Neurosciences
Yoshio Yamamoto, Kuniaki Sasaki, Misaki Komuro, Takuya Yokoyama, Sayed Sharif Abdali, Nobuaki Nakamuta
Summary: In the rat laryngeal mucosa, laminar nerve endings are distributed and activated by pressure changes in the laryngeal cavity. These nerve endings are suggested to play a role in efferent regulation and have secretory vesicles in the axoplasm. This study analyzed the laminar nerve endings in rats using 3D reconstruction and immunohistochemistry, revealing their interaction with Schwann cells and fibroblasts, as well as their attachment to epithelial cells. It was also found that these nerve endings release glutamate to maintain continuous discharge during the stretching of the laryngeal mucosa.
JOURNAL OF COMPARATIVE NEUROLOGY
(2023)
Article
Materials Science, Ceramics
Ali Sayir, Marie-Helene Berger, Tyson C. Back, Jonathan Mackey
Summary: The study investigates electron emission characteristics, electrical conductivity, formation of frameworks in polycrystalline mayenite, and its microstructure. It reveals a complex structure influenced by the incorporation of electrons using carbon ions, and the effect of different structures on electron emission. Additionally, the work function of the electride is determined and compared using different methods, showing a range of values and thermionic emission occurring at 600 degrees C.
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Daniela Anni, Eva-Maria Weiss, Debarpan Guhathakurta, Yagiz Enes Akdas, Julia Klueva, Stefanie Zeitler, Maria Andres-Alonso, Tobias Huth, Anna Fejtova
Summary: The study shows that Aβ 1-42 and Aβ 1-16 can increase the size of the recycling pool of synaptic vesicles, driven by enhanced endogenous cholinergic signaling.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Zhongfan Zheng, Xiumei Zhang, Junqiang Liu, Ping He, Shan Zhang, Yongning Zhang, Jie Gao, Shengmei Yang, Na Kang, Muhammad Irfan Afridi, Shangbang Gao, Chunhong Chen, Haijun Tu
Summary: The study demonstrates that GABAergic neurotransmission is crucial in regulating the resistance of nematodes to pathogens and is associated with the modulation of intestinal immunity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Aleksandra Polishchuk, Victor Cilleros-Mane, Laia Just-Borras, Marta Balanya-Segura, Genis Vandellos Pont, Carolina Silvera Simon, Marta Tomas, Neus Garcia, Josep Tomas, Maria A. Lanuza
Summary: Bidirectional communication between presynaptic and postsynaptic components at the neuromuscular synapse involves retrograde regulation of ACh release through phosphorylation of SNAP-25 and Synapsin-1. Muscle contraction leads to downregulation of pSynapsin-1 S9 and upregulation of pSNAP-25 T138, resulting in decreased neurotransmitter release.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)
Article
Neurosciences
Mohammed Al-Yaari, Chikao Onogi, Rei Yamada, Ryota Adachi, Daiya Kondo, Hiroshi Kuba
Summary: In this study, it was found that GABAergic inhibition in the avian nucleus magnocellularis is tonotopically regulated, with different characteristics in different tonotopic regions, contributing to the temporal coding of sound in the auditory nerve.
JOURNAL OF NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Dvyne Nosaka, Jeffery R. Wickens
Summary: Cholinergic interneurons of the striatum play a role in signaling by releasing acetylcholine. The prevailing theory suggests that these interneurons transmit signals through volume transmission rather than rapid synaptic transmission, as supported by evidence and mathematical modeling. The temporospatial distribution of acetylcholine is found to be short-range and short-lived, dominated by diffusion. The signaling is consistent with point-to-point transmission and allows faithful transmission of spike timing to the postsynaptic cell. Further evidence is needed for a better understanding of synaptic transmission by cholinergic interneurons in the striatum.
Article
Developmental Biology
Lorena Caipo, M. Constanza Gonzalez-Ramirez, Pablo Guzman-Palma, Esteban G. Contreras, Tomas Palominos, Nicolas Fuenzalida-Uribe, Bassem A. Hassan, Jorge M. Campusano, Jimena Sierralta, Carlos Oliva
DEVELOPMENTAL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Bryon Silva, Sergio Hidalgo, Jorge M. Campusano
Article
Biochemistry & Molecular Biology
Sergio Hidalgo, Christian Castro, Rafaella Zarate, Benjamin P. Valderrama, James J. L. Hodge, Jorge M. Campusano
NEUROCHEMISTRY INTERNATIONAL
(2020)
Article
Biochemistry & Molecular Biology
Sergio Hidalgo, Nicolas Fuenzalida-Uribe, Daniela Molina-Mateo, Angelica P. Escobar, Carlos Oliva, Rodrigo A. Espana, Maria Estela Andres, Jorge M. Campusano
Summary: This study introduces a method using fast scan cyclic voltammetry to record the release of endogenous amines in the adult fly brain. The research sheds light on how aminergic neural circuits influence behaviors in fruit flies, with findings showing an increase in octopamine and serotonin levels in response to electric shock and olfactory cues respectively in the calyx region associated with olfactory memory generation in flies.
JOURNAL OF NEUROCHEMISTRY
(2021)
Review
Behavioral Sciences
Angel Carvajal-Oliveros, Jorge M. Campusano
Summary: Serotonin functions as a neurotransmitter in the brain, regulating complex behaviors and neurodevelopment, with early alterations in its signaling linked to various neurodevelopmental disorders. Animal models may provide valuable insights into the complexity of serotonin actions and its role in brain disorders.
FRONTIERS IN BEHAVIORAL NEUROSCIENCE
(2021)
Article
Neurosciences
Sergio Hidalgo, Jorge M. Campusano, James J. L. Hodge
Summary: Schizophrenia has up to 80% heritability and is linked to specific genes such as CACNA1C, CACNA1I, and CACNA1G. Through research on fruit flies, it was found that loss of Ca(v)2 function may lead to sleep and behavioral deficits, as well as reduced cognitive abilities.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Multidisciplinary Sciences
Angel Carvajal-Oliveros, Carmen Dominguez-Baleon, Rafaella V. Zarate, Jorge M. Campusano, Veronica Narvaez-Padilla, Enrique Reynaud
Summary: Studies have found that nicotine has a protective effect on dopaminergic neurons in a fruit fly PD model, with its beneficial effects potentially linked to promoting neuron survival and metabolic function.
SCIENTIFIC REPORTS
(2021)
Article
Psychiatry
Sergio Hidalgo, Jorge M. Campusano, James J. L. Hodge
Summary: Schizophrenia has high heritability, with several genes associated with the disorder involved in calcium signalling and synaptic function. In this study, fruit fly mutants of the RIM1 orthologue RIM1 were used to model aspects of schizophrenia symptoms, demonstrating altered behaviors and synaptic changes associated with the disorder. Treatment with the antipsychotic haloperidol rescued locomotor deficits caused by Rim mutations.
TRANSLATIONAL PSYCHIATRY
(2021)
Article
Cell Biology
Pablo Guzman-Palma, Esteban G. Contreras, Natalia Mora, Macarena Smith, M. Constanza Gonzalez-Ramirez, Jorge M. Campusano, Jimena Sierralta, Bassem A. Hassan, Carlos Oliva
Summary: The Slit-Robo pathway is involved in the development of motion sensitive neurons in the Drosophila visual system, regulating processes such as neuronal precursor migration, cell segregation, and connectivity pattern formation. This signaling pathway plays a versatile role in different developmental stages of a neural lineage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Rafaella V. Zarate, Sergio Hidalgo, Nicole Navarro, Daniela Molina-Mateo, Duxan Arancibia, Francisca Rojo-Cortes, Carlos Oliva, Maria Estela Andres, Pedro Zamorano, Jorge M. Campusano
Summary: Parkinson's disease (PD) is a neurodegenerative disease characterized by motor symptoms and dopaminergic cell loss. This study shows that early dysfunction in the serotonergic system precedes and contributes to the onset of the Parkinsonian phenotype in Drosophila PD model. Manipulating the serotonergic system early on can prevent dopaminergic neuron loss and improve motor impairment.
Article
Biology
Francisca Rojo-Cortes, Nicolas Fuenzalida-Uribe, Victoria Tapia-Valladares, Candy B. Roa, Sergio Hidalgo, Maria-Constanza Gonzalez-Ramirez, Carlos Oliva, Jorge M. Campusano, Maria-Paz Marzolo
Review
Biochemistry & Molecular Biology
Ivana Gajardo, Simon Guerra, Jorge M. Campusano
Summary: Serotonin is a modulator that promotes changes in brain areas relevant to animal behavior, including sensory perception, learning, and memory. The role of serotonin in Drosophila's cognitive abilities, such as spatial navigation, is not well-studied. The literature suggests that serotonergic pathways in Drosophila modify various aspects of navigational memory formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Angel Carvajal-Oliveros, Carmen Dominguez-Baleon, Ivan Sanchez-Diaz, Diego Zambrano-Tipan, Rene Hernandez-Vargas, Jorge M. M. Campusano, Veronica Narvaez-Padilla, Enrique Reynaud
Summary: Synphilin-1, encoded by the SNCAIP gene, is associated with familial Parkinson's disease and is a major component of Lewy bodies in PD patients. Expression of Synphilin-1 in fruit flies induces neurodegeneration and PD-like symptoms. This study investigated the contribution of serotonergic and dopaminergic circuits in the development of PD-like phenotypes and found that olfactory and visual symptoms are primarily due to the serotonergic system, while motor symptoms and reduced survival are mainly contributed by the dopaminergic system. Chronic nicotine treatment was able to alleviate some of these symptoms, suggesting beneficial effects of nicotine on specific PD symptoms.
Meeting Abstract
Biochemistry & Molecular Biology
Jorge M. Campusano, M. Constanza Gonzalez, Carlos Oliva, M. Estela Andres, Isidora Almonacid
JOURNAL OF NEUROCHEMISTRY
(2023)
Meeting Abstract
Chemistry, Multidisciplinary
Sebastian Almendras, Angelica Fierro, Jorge Campusano, Jose Eltit, Edwin Perez
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2019)