期刊
DEVELOPMENTAL DYNAMICS
卷 240, 期 1, 页码 188-194出版社
WILEY
DOI: 10.1002/dvdy.22500
关键词
Wnt/PCP signaling; Dishevelled; Wnt5a; Wnt11; Frizzled7; Ror2; JNK; ATF2
资金
- Grants-in-Aid for Scientific Research [23710253] Funding Source: KAKEN
Non-canonical/planar cell polarity (PCP) Wnt signaling plays important roles in embryonic development and tissue homeostasis, and is implicated in human disease. Monitoring Wnt/PCP signaling relies mostly on semi-quantitative bioassays or biochemical analysis. Here we describe a luciferase reporter assay based on an ATF2 response element, which faithfully monitors non-canonical Wnt signaling in Xenopus embryos. The assay is simple, quantitative, and robust. It can be used to detect non-canonical Wnt signaling changes following gain and loss of function of pathway components, including Wnt, Frizzled, Ror2, Disheveled, Rac1, MKK7, and JNK. Wnt/PCP signaling has recently been implicated in left-right asymmetry and our reporter assay suggests that in gastrula embryos there is a right-ward bias in Wnt/PCP signaling. We also mapped Wnt/PCP signaling in the early Xenopus embryo and find that it peaks in the dorso-vegetal region, paralleling Wnt/beta-catenin signaling. Developmental Dynamics 240: 188-194, 2011. (C) 2010 WileyLiss, Inc.
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