Article
Cardiac & Cardiovascular Systems
Shi Ouyang, Wu-Ming Qin, Yu-Juan Niu, Yong-He Ding, Yun Deng
Summary: This study successfully constructed a novel zebrafish KI animal model to assess the effects of drugs on cardiac morphology. The findings revealed dose-dependent teratogenic effects of amantadine drug during early cardiogenesis, possibly conveyed by inhibiting the expression of key genes required for cardiac myocardium differentiation and LR asymmetry.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Cindy Y. Kok, Lauren M. MacLean, Renuka Rao, Shinya Tsurusaki, Eddy Kizana
Summary: The study aimed to identify a gene that can be delivered to the heart to protect against cancer therapy-induced cardiotoxicity. By replacing the CMV promoter with the cardiac-specific NCX1 promoter, a lentiviral vector capable of inducing the expression of cardioprotective gene Bcl-2 was successfully produced.
Article
Agriculture, Dairy & Animal Science
Hyeon Yang, Bo Ram Lee, Hwi-Cheul Lee, Hoonsung Choi, Sun Keun Jung, Ji-Youn Kim, Jingu No, Sureshkumar Shanmugam, Yong Jin Jo, Keon Bong Oh, Kyung Woon Kim, Sung June Byun
Summary: Virus injection into EGK-X embryos is a common method in avian transgenesis, using a chicken ovalbumin gene promoter to induce gene expression. Recombinant promoters linking regulatory regions of ovalbumin and other oviduct-specific genes have shown increased luciferase activity in chicken oviduct cells, suggesting they may be a more efficient way to drive trans-gene expression in transgenic chickens.
Article
Fisheries
Charles B. Kimmel, Alexander L. Wind, Whitney Oliva, Samuel D. Ahlquist, Charline Walker, John Dowd, Bernardo Blanco-Sanchez, Tom A. Titus, Peter Batzel, Jared C. Talbot, John H. Postlethwait, James T. Nichols
Summary: The study reveals that transgenes can significantly impact genome function, particularly in phenotypes related to neural crest-derived craniofacial skeleton, without causing widespread changes. The influence of the transgene appears to be genetically dominant, affecting specific traits in a unique manner.
JOURNAL OF FISH BIOLOGY
(2021)
Article
Developmental Biology
Yiting Gui, Yawen Zhang, Qi Zhang, Xudong Chen, Feng Wang, Fang Wu, Yonghao Gui, Qiang Li
Summary: This study identified functionally conserved sequences and the core sequence ECR2 in the upstream region of the Fugu tnni1a gene that affect heart-specific expression.
CELLS & DEVELOPMENT
(2022)
Review
Cardiac & Cardiovascular Systems
Yao Yao, Amanda N. Marra, Deborah Yelon
Summary: The identity of cardiac chambers in the developing heart is determined by signals in early embryo, triggering distinct differentiation pathways. The zebrafish model has revealed factors influencing chamber fate specification and commitment, providing insights for future strategies in cardiac tissue engineering.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2021)
Article
Clinical Neurology
Bradley S. Hollidge, Hayley B. Carroll, Randolph Qian, Madison L. Fuller, April R. Giles, Andrew C. Mercer, Olivier Danos, Ye Liu, Joseph T. Bruder, Jared B. Smith
Summary: Understanding the kinetics and durability of AAV-mediated transgene expression in the brain is crucial for basic neuroscience studies and gene therapy approaches for CNS diseases. This study characterizes the temporal profile of transgene expression after AAV injections in the mouse striatum and compares the efficacy of different promoters. The results reveal that the CAG promoter shows the highest initial expression, while the hSyn promoter demonstrates long-term durability. Additionally, the processing of vector genomes into circular episomes and concatemers is a dynamic process that continues up to 3 months post-injection.
FRONTIERS IN NEUROLOGY
(2022)
Article
Environmental Sciences
Meifeng Li, Tingting Yu, Jingli Lai, Xue Han, Jihuan Hu, Zeyuan Deng, Dongming Li, Zuocheng Ye, Shanghong Wang, Chengyu Hu, Xiaowen Xu
Summary: The study revealed that ethoprophos exposure can cause cardiac defects in zebrafish embryos, such as low heart rate, pericardium edema, and long SV-BA distance, while not affecting vascular development. This indicates that oxidative stress and inhibition of the wnt signaling pathway are the molecular mechanisms underlying ethoprophos-induced injury in zebrafish.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Cardiac & Cardiovascular Systems
Bohan Zhang, Kristofor E. Pas, Toluwani Ijaseun, Hung Cao, Peng Fei, Juhyun Lee
Summary: The study introduces advanced deep-learning techniques as a rapid and accurate method for segmenting the inner volume of zebrafish hearts, offering an efficient tool for quantitative research during cardiac development.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cell Biology
Zhentao Zhang, Jesse Villalpando, Wenhui Zhang, Young-Jae Nam
Summary: Forced expression of core cardiogenic transcription factors can reprogram fibroblasts to induced cardiomyocyte-like cells, showing potential for heart regeneration. However, it is uncertain whether chamber-specific cardiomyocytes can be generated through this approach. In vivo cardiac reprogramming post-myocardial infarction induces a ventricular-like phenotype, while in vitro generated iCMs fail to determine their chamber identities, suggesting a potential advantage of in vivo cardiac reprogramming for chamber-matched new cardiomyocyte generation.
Article
Plant Sciences
Erik Carlson, Kristen Stewart, Kathleen Baier, Linda McGuigan, Tobi Culpepper, William Powell
Summary: Genetically engineered American chestnut expressing wheat oxalate oxidase has been developed to increase tolerance to chestnut blight caused by Cryphonectria parasitica. Transgenic chestnut lines with the win3.12 inducible promoter from poplar driving OxO expression showed elevated disease tolerance similar to blight-resistant Chinese chestnut controls, with targeted expression to wounded and infected tissue.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Environmental Sciences
Yunlong Meng, Keyuan Zhong, Suping Chen, Yong Huang, You Wei, Juan Wu, Juan Liu, Zhaopeng Xu, Jing Guo, Fasheng Liu, Huiqiang Lu
Summary: The study revealed that exposure to Pendimethalin can reduce heart rate, survival rate, and body length of zebrafish embryos, causing various malformations and affecting antioxidant enzyme activity and the expression of genes related to cardiac development.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Multidisciplinary Sciences
Amedee Mollard, Cecile Peccate, Anne Forand, Julie Chassagne, Laura Julien, Pierre Meunier, Zoheir Guesmia, Thibaut Marais, Marc Bitoun, France Pietri-Rouxel, Sofia Benkhelifa-Ziyyat, Stephanie Lorain
Summary: The study focused on the impact of the necrosis-regeneration process on the efficiency of AAV therapy, finding that regenerated muscles showed reduced transgene expression, which may limit the application of AAV-based therapies.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Jinyu Kang, Lujie Hang, Wentao Zheng, Jia Luo, Xie Zhang, Yufei Song, Aiming Liu
Summary: This study compared the transduction efficiency of rAAV8 vectors with different promoters in the liver following IV and IP injections. The results showed that the CAG promoter exhibited the highest efficiency in both IV and IP injections. IV administration with the CAG promoter resulted in 67-fold higher EGFP expression compared to the TBG410 promoter group and 26-fold higher compared to the TBG669 promoter group. Both CAG and TBG669 promoters showed higher EGFP protein expression with IV injection compared to IP injection. With the CAG promoter, IV injection resulted in 1.5-fold higher EGFP protein expression compared to IP injection. No differences were observed between the two administrations with the TBG410 promoter.
MOLECULAR MEDICINE REPORTS
(2022)
Review
Cell Biology
Rui Gao, Jie Ren
Summary: The review discusses the cellular and molecular mechanisms of cardiac conduction system (CCS) development and how the zebrafish model has been utilized to dissect these mechanisms. Evolutionarily conserved developmental mechanisms discovered in zebrafish could potentially contribute to better treatment for patients with cardiac conduction disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)