Article
Multidisciplinary Sciences
Liesbeth Gebuijs, Frank A. Wagener, Jan Zethof, Carine E. Carels, Johannes W. Von den Hoff, Juriaan R. Metz
Summary: This study investigates the effects of mutated fgfr2 allele on cartilage and bone formation in zebrafish. The findings suggest that pharmacological targeting of FGFR1-4 kinase signaling causes severe craniofacial malformations.
Article
Developmental Biology
Soma Dash, Paul A. Trainor
Summary: This study reveals the tissue-specific functions of Nucleolin in rRNA transcription, post-transcriptional regulation, and embryonic craniofacial development.
Article
Cell Biology
Zhaohui Wei, Qiang Hong, Zijiao Ding, Jingwen Liu
Summary: The study generated mutant lines for cxcl12a and cxcr4b using the CRISPR/Cas9 system. Results showed that Cxcl12a is crucial for pharyngeal cartilage formation by promoting the proliferation of craniofacial neural crest cells. Additionally, Cxcl12a and Cxcl12b were found to have a synergistic influence on pharyngeal arch and pouch formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Marie Berenguer, Gregg Duester
Summary: The role of retinoic acid (RA) and Meis1/2 in limb development is complex, with RA activating Meis1/2 in the proximal limb bud mesoderm while FGF8 represses Meis1/2 in the distal limb to generate proximodistal patterning.
Article
Developmental Biology
Hui Wang, Chengdong Wang, Qi Long, Yuan Zhang, Meiling Wang, Jie Liu, Xufeng Qi, Dongqing Cai, Gang Lu, Jianmin Sun, Yong-Gang Yao, Wood Yee Chan, Wai Yee Chan, Yi Deng, Hui Zhao
Summary: Kindlin2 is essential for neural crest formation in Xenopus embryos independently of integrins, and it regulates the FGF pathway to promote the stability of FGF receptor 1.
Article
Environmental Sciences
Zekun Li, Kun Jia, Xiaomei Chen, Jun Guo, Zhiguo Zheng, Weihua Chen, Yuan Peng, Yuhao Yang, Huiqiang Lu, Jian Yang
Summary: This study investigated the developmental toxicity of Butylparaben (BuP) in zebrafish embryos, and found that exposure to BuP caused severe craniofacial deformities, periocular edema, cardiac dysplasia, and delayed otolith development in zebrafish larvae. The oxidative stress response was enhanced, and the activities of catalase and superoxide dismutase were reduced while the concentration of malondialdehyde was elevated. In addition, ALP activity and the expression of chondrocyte marker genes were decreased. These findings indicate that BuP-induced oxidative stress inhibits the proliferation of neural crest cells (NCCs), leading to craniofacial deformities.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Megan Leask, Catherine Carleton, Bryony Leeke, Trent Newman, Joseph Antoun, Mauro Farella, Julia Horsfield
Summary: This study explores the impact of oxidative stress on neural crest cells, leading to craniofacial abnormalities, and tests the therapeutic effect of antioxidants on these effects, suggesting that antioxidants may potentially prevent defects in craniofacial cartilage.
Article
Anatomy & Morphology
Stefan Washausen, Wolfgang Knabe
Summary: Using various methods, this study reveals that senescence and apoptosis contribute to the development of branchial arches, epibranchial placodes, and pharyngeal pouches in mice. These processes play a role in the invagination of branchial clefts, deepening of the cervical sinus floor, induction of pit indentation, regulation of Pax8(+) precursors, narrowing of neuroblast delamination sites, and regression of placodes. The putative signaling centers in the pharyngeal pouches are likely deactivated by senescence and apoptosis.
DEVELOPMENTAL DYNAMICS
(2023)
Review
Biochemistry & Molecular Biology
Jaroslav Fabik, Viktorie Psutkova, Ondrej Machon
Summary: This review focuses on the craniofacial development in mouse and zebrafish models, highlighting the gene regulatory networks directing the patterning and osteochondrogenesis of the mandibular and hyoid arches. The study identifies that mandibular malformations often co-occur with hyoid malformations in humans and mice, and the dysregulation of molecular signalling influences the development of skeletal components of the viscerocranium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Xiaomin Niu, Fuyu Zhang, Lu Ping, Yibei Wang, Bo Zhang, Jian Wang, Xiaowei Chen
Summary: This study investigates the molecular effects of a VWA1 mutation on the development of hemifacial microsomia (HFM) using a zebrafish model. The mutation results in cartilage dysmorphologies and abnormalities in cranial neural crest cell (CNCC) condensation, differentiation, proliferation, and apoptosis. Additionally, the VWA1 gene appears to regulate the FGF signaling pathway.
Article
Surgery
Sara Toro-Tobon, Monica Manrique, Juliana Paredes-Gutierrez, Esperanza Mantilla-Rivas, Haley Oh, Laiba Ahmad, Albert K. Oh, Gary F. Rogers
Summary: The pharyngeal arches are crucial for the development of the face and neck, and understanding their normal and pathological development is paramount for accurate diagnosis and treatment.
JOURNAL OF CRANIOFACIAL SURGERY
(2023)
Article
Biochemistry & Molecular Biology
Omran Karmach, Joseph Madrid, Subham Dasgupta, David C. Volz, Nicole zur Nieden
Summary: Cigarette smoke exposure is the main preventable cause of death and disease in the United States. Secondhand smoke can harm pregnant women and developing embryos, leading to issues with bone mineralization and craniofacial defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Emily P. Y. Yu, Vishal Saxena, Sofia Perin, Marc Ekker
Summary: The dlx genes encode transcription factors that play important roles in craniofacial development. Mutations in dlx5a and dlx5i6 were found to lead to shortened cartilage lengths and altered proliferation of neural crest cells. These mutations also affected the non-canonical Wnt signaling pathway.
Article
Physiology
Haoran Zhang, Junjie Xie, Karl Kam Hei So, Ka Kui Tong, Jearn Jang Sae-Pang, Li Wang, Sze Lan Tsang, Wood Yee Chan, Elaine Yee Man Wong, Mai Har Sham
Summary: This study demonstrates that Hoxb3 regulates Jag1 expression, affecting the normal development of craniofacial structures, particularly the growth of pharyngeal arches and migration of neural crest cells. Molecular experiments showed that Hoxb3 directly regulates Jag1 expression, with elevated expression in the pharyngeal epithelium leading to abnormal cellular interactions and a deficiency in neural crest cells migrating into PA2.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shuyan Yang, Xin Xu, Zheng Yin, Yuelin Liu, Handong Wang, Jin Guo, Fang Wang, Yihua Bao, Ting Zhang, Shaoguang Sun
Summary: In this study, the researchers investigated the role of Nkx2.3 in the development of posterior ceratobranchial cartilages in zebrafish. They found that the absence of Nkx2.3 resulted in the absence of posterior pharyngeal cartilages, which was due to compromised proliferation, differentiation, and survival of cranial neural crest cells. The study also suggested that Nkx2.3 negatively regulated Fgf signaling and that inhibiting Fgf signaling could restore the abnormalities in posterior cartilages induced by Nkx2.3 knockdown.
Editorial Material
Developmental Biology
Kristin Bruk Artinger, Dominique Alfandari, Lisa A. Taneyhill
MECHANISMS OF DEVELOPMENT
(2017)
Article
Developmental Biology
Jason S. Williams, Jessica Y. Hsu, Christy Cortez Rossi, Kristin Bruk Artinger
DEVELOPMENTAL BIOLOGY
(2018)
Article
Developmental Biology
Lomeli Carpio Shull, Rwik Sen, Johannes Menzel, Susumu Goyama, Mineo Kurokawa, Kristin Bruk Artinger
DEVELOPMENTAL BIOLOGY
(2020)
Article
Developmental Biology
Sofia A. Pezoa, Kristin B. Artinger, Lee A. Niswander
DEVELOPMENTAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Ritsuko Iwanaga, Brittany T. Truong, Jessica Y. Hsu, Karoline A. Lambert, Rajesh Vyas, David Orlicky, Yiqun G. Shellman, Aik-Choon Tan, Craig Ceol, Kristin Bruk Artinger
MOLECULAR CARCINOGENESIS
(2020)
Editorial Material
Developmental Biology
Kristin Bruk Artinger
BIRTH DEFECTS RESEARCH
(2020)
Review
Developmental Biology
Brittany T. Truong, Kristin B. Artinger
Summary: Even though craniofacial and limb defects appear to have distinct gene regulatory networks, they often co-occur, suggesting commonalities between these two types of congenital anomalies. Using zebrafish models, studies can delve into the mechanisms of human syndromes to gain a deeper understanding of the underlying causes of these deformities.
Article
Biology
Ezra Lencer, Rytis Prekeris, Kristin Bruk Artinger
Summary: This study utilized single-cell RNA sequencing to characterize transcriptional changes associated with neural crest cell development in the zebrafish trunk during the early stages of migration. The findings revealed transcriptional diversity in neural crest cells, early expression of genes associated with differentiated derivatives in pre-migratory stages, and the identification of a population of Rohon-Beard neurons. The data presented provide novel genetic markers for multiple trunk neural crest cell populations and Rohon-Beard neurons, shedding light on previously uncharacterized genes critical for vertebrate development.
Article
Developmental Biology
Lomeli C. Shull, Ezra S. Lencer, Hyun Min Kim, Susumu Goyama, Mineo Kurokawa, James C. Costello, Kenneth Jones, Kristin B. Artinger
Summary: This study demonstrates that transcription factors and histone methyltransferase proteins Prdm3 and Prdm16 play crucial roles in the differentiation switch of cranial neural crest cells (NCCs) to craniofacial cartilage. The loss of either protein leads to abnormal chondrocyte development, which is regulated by controlling the timing of Wnt/beta-catenin activity and chromatin accessibility. Manipulating Wnt/beta-catenin signaling or generating double mutants of prdm3(-/-) and prdm16(-/-) can rescue craniofacial cartilage defects.
Article
Cell Biology
Jessica Y. Hsu, Etienne P. Danis, Stephanie Nance, Jenean H. O'Brien, Annika L. Gustafson, Veronica M. Wessells, Andrew E. Goodspeed, Jared C. Talbot, Sharon L. Amacher, Paul Jedlicka, Joshua C. Black, James C. Costello, Adam D. Durbin, Kristin B. Artinger, Heide L. Ford
Summary: This study demonstrates the crucial role of SIX1 in maintaining the undifferentiated state of rhabdomyosarcoma (RMS) and inhibiting tumor growth. SIX1 controls enhancer activity and MYOD1 occupancy at specific loci to preserve the undifferentiated state of RMS cells.
Letter
Developmental Biology
Kristin B. Artinger, Michiko Watanabe
BIRTH DEFECTS RESEARCH
(2022)
Editorial Material
Developmental Biology
Poorni Iyer, Michiko Watanabe, Kristin B. Artinger
BIRTH DEFECTS RESEARCH
(2023)
Article
Developmental Biology
Ezra Lencer, Addison Rains, Erin Binne, Rytis Prekeris, Kristin B. Artinger
Summary: The transmembrane proteins cdon and boc are involved in regulating hedgehog signaling and have additional roles in axon guidance and neural crest cell migration. The study investigates the function of cdon and boc in zebrafish neural crest cell migration using mutants and finds disruption of migration in double cdon;boc mutants. The study also reveals defects in slow-twitch muscle cell differentiation and extracellular matrix loss, suggesting that neural crest defects may be secondary to mesoderm development defects.
Review
Developmental Biology
Lomeli C. Shull, Kristin B. Artinger
Summary: In this review, the role of epigenetic factors during neural crest development, specifically in craniofacial development, is discussed, as well as how compromised activities of these regulators contribute to congenital defects affecting the craniofacial complex.
BIRTH DEFECTS RESEARCH
(2023)
Article
Developmental Biology
Rwik Sen, Sofia A. Pezoa, Lomeli Carpio Shull, Laura Hernandez-Lagunas, Lee A. Niswander, Kristin Bruk Artinger
JOURNAL OF DEVELOPMENTAL BIOLOGY
(2018)