Article
Biochemistry & Molecular Biology
Muriel Rigolet, Nicolas Buisine, Marylou Scharwatt, Evelyne Duvernois-Berthet, Daniel R. Buchholz, Laurent M. Sachs
Summary: Thyroid hormones and glucocorticoids play a role in cell-cycle regulation through the joint regulation of genes in the liver of tadpoles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shouhong Wang, Yuki Shibata, Liezhen Fu, Yuta Tanizaki, Nga Luu, Lingyu Bao, Zhaoyi Peng, Yun-Bo Shi
Summary: Animal regeneration is a natural process that relies on the level of thyroid hormone (T3), which is responsible for the loss of regenerative ability in many organs during development. The metamorphic stages in amphibians, similar to postembryonic development in humans, provide a good model to study the gradual loss of regenerative ability and the role of T3 in this process.
CELL AND BIOSCIENCE
(2023)
Review
Endocrinology & Metabolism
Yun-Bo Shi
Summary: Thyroid hormone T3 plays a critical role in organ function, metabolism, and animal development, especially during the neonatal period in mammals. Studies on anuran metamorphosis in amphibians have shed light on how T3 regulates vertebrate development. The absence of T3 receptors in tadpoles leads to premature development of adult tissues and eventually lethality, highlighting the importance of TRs in mediating T3 effects.
Review
Endocrinology & Metabolism
Yuta Tanizaki, Yuki Shibata, Wonho Na, Yun-Bo Shi
Summary: Amphibian metamorphosis, especially in anurans like Xenopus laevis and Xenopus tropicalis, is a valuable model to study how T3 regulates adult organ development due to its similarity to mammalian postembryonic development and its independence from maternal influence. Studies on intestinal remodeling have provided important insights into the molecular mechanisms of T3 regulation of cell fate during development, suggesting that T3-induced activation of the cell cycle program is crucial for larval epithelial cell death and the formation of adult intestinal stem cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Mengxi Chen, Takehiro Tanaka, Takuro Igawa, Yanyan Han, Fangli Peng, Zaishun Jin, Tadashi Yoshino
Summary: An ectopic pancreas refers to pancreas tissue that is located outside its normal position and anatomically separated from the pancreas. The transcription factors PDX1, PTF1A, and SALL4 play important roles in pancreatic development and differentiation. In this study, the expression of PDX1, PTF1A, and SALL4 in ectopic pancreas was analyzed, and it was found that PTF1A promotes exocrine differentiation, while SALL4 is involved in self-renewal and differentiation. These findings provide insights into the mechanisms underlying ectopic pancreas formation and differentiation.
Article
Cell Biology
Nicolas Buisine, Alexis Grimaldi, Vincent Jonchere, Muriel Rigolet, Corinne Blugeon, Juliette Hamroune, Laurent Marc Sachs
Summary: The study focused on the interactions between thyroid hormone and glucocorticoid signaling during Xenopus tropicalis metamorphosis, revealing that the cross-talks are more complex than initially thought. Gene expression is mainly regulated by T-3 or CORT, with some genes showing intricate interactions between the signaling pathways. DNA methylation changes are highly dynamic and influenced by cross-talks.
Article
Biochemistry & Molecular Biology
Yuta Tanizaki, Yuki Shibata, Hongen Zhang, Yun-Bo Shi
Summary: Thyroid hormone receptors, specifically TRα, play a crucial role in limb development by directly binding to target genes involved in cell cycle and Wnt signaling pathways. They prevent precocious limb formation before metamorphosis and promote limb development during the metamorphic process through T3 activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Yuta Tanizaki, Lingyu Bao, Bingyin Shi, Yun-Bo Shi
Summary: The thyroid hormone T3 plays a crucial role in vertebrate development, cellular processes, and metabolism through the T3 receptor (TR) pathway. In this study, researchers used Xenopus tropicalis animals lacking a functional SRC3 gene to demonstrate the important role of SRC3 in intestinal remodeling during postembryonic development. The findings show that SRC3 is a critical component of the TR-signaling pathway in vivo during intestinal remodeling, particularly affecting adult intestinal stem cell proliferation and larval epithelial cell apoptosis.
Article
Endocrinology & Metabolism
Yuta Tanizaki, Yuki Shibata, Hongen Zhang, Yun-Bo Shi
Summary: The study demonstrates a close relationship between TR alpha-regulated T3 activation of the cell cycle program and larval epithelial cell death and adult epithelial stem cell development during intestinal remodeling in Xenopus tropicalis metamorphosis.
Article
Endocrinology & Metabolism
Bidisha Paul, Rejenae Dockery, Valery M. Valverde, Daniel R. Buchholz
Summary: Corticosteroids play a critical role in development and stress responses in vertebrates. A new gene called frzb has been identified as a marker for corticosterone response in frog tadpole tails, making it useful for hormone treatments and endocrine disruption studies.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Rikke Poulsen, Shireen H. Jackman, Martin Hansen, Caren C. Helbing
Summary: Anuran metamorphosis is associated with changes in thyroid hormone levels and gene expression. New technology allows for measuring TH levels and gene transcription during metamorphosis. The concentration profile of T2 during metamorphosis suggests its role in anuran development.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Environmental Sciences
ZhiChao Dang
Summary: Both AMA and LAGDA are used to detect thyroid-mediated modality and adversity based on morphological changes during thyroid hormone-dependent metamorphosis. The EU Guidance recommends their use for investigating the effects of thyroid hormone system disrupting chemicals. However, there is debate over the necessity of using LAGDA as a follow-up to positive AMA results. This study analyzes the similarities, differences, and sensitivity of these two assays in detecting TDCs.
ENVIRONMENTAL POLLUTION
(2022)
Article
Cell Biology
Yuki Shibata, Yuta Tanizaki, Hongen Zhang, Hangnoh Lee, Mary Dasso, Yun-Bo Shi
Summary: Research shows that TR genes play crucial roles in larval epithelial cell death and adult stem cell formation during anuran metamorphosis. Lack of these processes in TRDKO tadpoles results in inhibition of metamorphosis progression.
Article
Endocrinology & Metabolism
Liezhen Fu, Robert Liu, Vincent Ma, Yun-Bo Shi
Summary: Thyroid hormone T3 plays an important role in adult organ function and vertebrate development. Amphibian metamorphosis, which is dependent on T3, offers a unique opportunity to study postembryonic development in vertebrates. The proto-oncogene Ski is a direct target gene of T3 and its expression peaks during the climax of metamorphosis in Xenopus tropicalis tadpoles.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2022)
Article
Marine & Freshwater Biology
Wei Zhou, Zhan-Fen Qin, Yuan-Yuan Li, Jin-Bo Li, Ya-li Shi, Miao-xin Dong, Xiang Li, Yong-Jun Zhang, Yi-De He
Summary: Thyroid disrupting chemicals (TDCs) have become a major concern for their potential adverse effects on animal and human health. The developed TiXMA assay has shown effectiveness in detecting TDCs that disrupt thyroid hormone signaling. The study expands the application of TiXMA to screen TDCs that interfere with the hypothalamic-pituitary-thyroid (HPT) axis, and the results demonstrate its capability in screening such TDCs with a shorter exposure duration compared to the AMA assay.
AQUATIC TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Makoto Nakamura, Hitoshi Yoshida, Eri Takahashi, Marcin Wlizla, Kimiko Takebayashi-Suzuki, Marko E. Horb, Atsushi Suzuki
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Cell Biology
Jean A. Smith, Ennessa G. Curry, R. Eric Blue, Christine Roden, Samantha E. R. Dundon, Anthony Rodriguez-Vargas, Danielle C. Jordan, Xiaomin Chen, Shawn M. Lyons, John Crutchley, Paul Anderson, Marko E. Horb, Amy S. Gladfelter, Jimena Giudice
JOURNAL OF CELL BIOLOGY
(2020)
Article
Pharmacology & Pharmacy
Nathalie Percie du Sert, Viki Hurst, Amrita Ahluwalia, Sabina Alam, Marc T. Avey, Monya Baker, William J. Browne, Alejandra Clark, Innes C. Cuthill, Ulrich Dirnagl, Michael Emerson, Paul Garner, Stephen T. Holgate, David W. Howells, Natasha A. Karp, Stanley E. Lazic, Katie Lidster, Catriona J. MacCallum, Malcolm Macleod, Esther J. Pearl, Ole H. Petersen, Frances Rawle, Penny Reynolds, Kieron Rooney, Emily S. Sena, Shai D. Silberberg, Thomas Steckler, Hanno Wurbel
BRITISH JOURNAL OF PHARMACOLOGY
(2020)
Article
Physiology
Nathalie Percie du Sert, Viki Hurst, Amrita Ahluwalia, Sabina Alam, Marc T. Avey, Monya Baker, William J. Browne, Alejandra Clark, Innes C. Cuthill, Ulrich Dirnagl, Michael Emerson, Paul Garner, Stephen T. Holgate, David W. Howells, Natasha A. Karp, Stanley E. Lazic, Katie Lidster, Catriona J. MacCallum, Malcolm Macleod, Esther J. Pearl, Ole H. Petersen, Frances Rawle, Penny Reynolds, Kieron Rooney, Emily S. Sena, Shai D. Silberberg, Thomas Steckler, Hanno Wurbel
EXPERIMENTAL PHYSIOLOGY
(2020)
Article
Endocrinology & Metabolism
Nathalie Percie du Sert, Viki Hurst, Amrita Ahluwalia, Sabina Alam, Marc T. Avey, Monya Baker, William J. Browne, Alejandra Clark, Innes C. Cuthill, Ulrich Dirnagl, Michael Emerson, Paul Garner, Stephen T. Holgate, David W. Howells, Natasha A. Karp, Stanley E. Lazic, Katie Lidster, Catriona J. MacCallum, Malcolm Macleod, Esther J. Pearl, Ole H. Petersen, Frances Rawle, Penny Reynolds, Kieron Rooney, Emily S. Sena, Shai D. Silberberg, Thomas Steckler, Hanno Wurbel
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2020)
Editorial Material
Neurosciences
Nathalie Percie du Sert, Viki Hurst, Amrita Ahluwalia, Sabina Alam, Marc T. Avey, Monya Baker, William J. Browne, Alejandra Clark, Innes C. Cuthill, Ulrich Dirnagl, Michael Emerson, Paul Garner, Stephen T. Holgate, David W. Howells, Natasha A. Karp, Stanley E. Lazic, Katie Lidster, Catriona J. MacCallum, Malcolm Macleod, Esther J. Pearl, Ole H. Petersen, Frances Rawle, Penny Reynolds, Kieron Rooney, Emily S. Sena, Shai D. Silberberg, Thomas Steckler, Hanno Wurbel
JOURNAL OF PHYSIOLOGY-LONDON
(2020)
Article
Multidisciplinary Sciences
Allan F. Wiechmann, Teryn A. Martin, Marko E. Horb
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Nathalie Percie du Sert, Amrita Ahluwalia, Sabina Alam, Marc T. Avey, Monya Baker, William J. Browne, Alejandra Clark, Innes C. Cuthill, Ulrich Dirnagl, Michael Emerson, Paul Garner, Stephen T. Holgate, David W. Howells, Viki Hurst, Natasha A. Karp, Stanley E. Lazic, Katie Lidster, Catriona J. MacCallum, Malcolm Macleod, Esther J. Pearl, Ole H. Petersen, Frances Rawle, Penny Reynolds, Kieron Rooney, Emily S. Sena, Shai D. Silberberg, Thomas Steckler, Hanno Wuerbel
Article
Multidisciplinary Sciences
Thomas Naert, Dieter Tulkens, Nicole A. Edwards, Marjolein Carron, Nikko-Ideen Shaidani, Marcin Wlizla, Annekatrien Boel, Suzan Demuynck, Marko E. Horb, Paul Coucke, Andy Willaert, Aaron M. Zorn, Kris Vleminckx
SCIENTIFIC REPORTS
(2020)
Article
Developmental Biology
Kelsey Coppenrath, Andre L. P. Tavares, Nikko-Ideen Shaidani, Marcin Wlizla, Sally A. Moody, Marko Horb
Summary: The Six1 gene plays a crucial role in vertebrate organ development, and its mutation leads to severe disruptions in the morphology of multiple organs and contributes to the formation of related diseases.
Article
Developmental Biology
Natalya Zahn, Christina James-Zorn, Virgilio G. Ponferrada, Dany S. Adams, Julia Grzymkowski, Daniel R. Buchholz, Nanette M. Nascone-Yoder, Marko Horb, Sally A. Moody, Peter D. Vize, Aaron M. Zorn
Summary: Normal tables and illustrations of embryonic development play a crucial role in research and teaching. However, due to copyright restrictions, the use of classic illustrations is no longer possible. To address this, we have created a new resource consisting of 133 high-quality illustrations and a landmarks table, which will support future Xenopus research and teaching.
Article
Developmental Biology
Douglas W. Houston, Karen L. Elliott, Kelsey Coppenrath, Marcin Wlizla, Marko E. Horb
Summary: Asymmetric signalling centres are crucial for early axis formation in vertebrates. The study finds that Wnt11b is involved in the distribution of dorsal determinants and plays a key role in dorsal axis formation and embryonic development.
Article
Biochemistry & Molecular Biology
Natasha A. Karp, Esther J. Pearl, Emma J. Stringer, Chris Barkus, Jane Coates Ulrichsen, Nathalie Percie du Sert
Summary: This study explores the implementation and barriers of blinding methods in animal experiments. The lack of blinding is shown to lead to an overestimation of treatment efficacy and false positive findings. The study identifies operational and informatic systems as significant barriers and highlights challenges related to knowledge and work culture regarding blinding.
Article
Biochemistry & Molecular Biology
Marco Morselli, Ronan Bennett, Nikko-Ideen Shaidani, Marko Horb, Leonid Peshkin, Matteo Pellegrini
Summary: This study finds that age-associated changes in DNA methylation also exist in amphibians, and whole-genome bisulfite sequencing (WGBS) profiles from skin samples of nine frogs were used to characterize the gene- and chromosome-scale features of age-associated DNA methylation changes. The results suggest that the mechanism of DNA methylation changes in amphibians is similar to that in mammals. Additionally, a targeted DNA methylation analysis (TBSseq) assay was developed for future studies with larger cohorts of individuals. The findings of this study will contribute to understanding the relationship between DNA methylation and other hallmarks of aging using the unique resources available for Xenopus.
Article
Genetics & Heredity
Benjamin L. S. Furman, Caroline M. S. Cauret, Martin Knytl, Xue-Ying Song, Tharindu Premachandra, Caleb Ofori-Boateng, Danielle C. Jordan, Marko E. Horb, Ben J. Evans
