期刊
DEVELOPMENTAL DYNAMICS
卷 237, 期 7, 页码 1923-1934出版社
WILEY
DOI: 10.1002/dvdy.21608
关键词
Shh; blood vessels; branchial arches; 5E1 hybridoma cells; quail embryo; endothelium
资金
- NICHD NIH HHS [1R01HD042307] Funding Source: Medline
- NIGMS NIH HHS [R01 GM072207-04, R01 GM072207] Funding Source: Medline
Sonic hedgehog (Shh) is a morphogen involved in many developmental processes. Injection of cells (5E1) that produce a Shh-blocking antibody causes an attenuation of the Shh response, and this causes vascular malformations and impaired remodeling characterized by hemorrhages and protrusions of the anterior cardinal vein and outflow tract, delayed fusion of the dorsal aortae, impaired branching of the internal carotid artery, and delayed remodeling of the aortic arches. Distribution of smooth muscle cells in the vessel wall is unchanged. In 5E1-injected embryos, we also observed impaired assembly of endothelial cells into vascular tubes, particularly in the sixth branchial arch, around the anterior cardinal vein and around the dorsal aorta. In 5E1-treated embryos, increased numbers of macrophage-like cells, apoptotic cells, and a decreased level of proliferation were observed in head mesenchyme. Together, these observations show that Shh signaling is required at multiple stages for proper vessel formation and remodeling.
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