期刊
DEVELOPMENTAL CELL
卷 27, 期 5, 页码 574-585出版社
CELL PRESS
DOI: 10.1016/j.devcel.2013.10.023
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资金
- Royal Dutch Academy of Arts and Sciences (KNAW)
- Dutch Cancer Society (KWF)
- Cancer Genomics Centre
- European Union
- Netherlands Organization for Scientific Research (NWO)
- Netherlands Proteomics Center (NPC)
Epithelial cell migration is crucial for the development and regeneration of epithelial tissues. Aberrant regulation of epithelial cell migration has a major role in pathological processes such as the development of cancer metastasis and tissue fibrosis. Here, we report that in response to factors that promote cell motility, the Rap guanine exchange factor RAPGEF2 is rapidly phosphorylated by I-kappa-B-kinase-beta and casein kinase-1 alpha and consequently degraded by the proteasome via the SCF beta TrCP ubiquitin ligase. Failure to degrade RAPGEF2 in epithelial cells results in sustained activity of Rap1 and inhibition of cell migration induced by HGF, a potent metastatic factor. Furthermore, expression of a degradation-resistant RAPGEF2 mutant greatly suppresses dissemination and metastasis of human breast cancer cells. These findings reveal a molecular mechanism regulating migration and invasion of epithelial cells and establish a key direct link between IKK beta and cell motility controlled by Rap-integrin signaling.
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