Article
Biochemistry & Molecular Biology
Pooja Kumari, Lars Harald Thuestad, Rafal Ciosk
Summary: The LIN-41/TRIM71 protein in Caenorhabditis elegans serves as a versatile regulator of mRNA fate, playing different roles in somatic and germline cells. In somatic cells, LIN-41 recognizes specific mRNA sequences and either triggers decay or represses translation. In the germline, LIN-41's targets and regulatory mechanisms are not well understood, but it has been suggested to indirectly control mRNA by interacting with other RNA-binding proteins. This study demonstrates that LIN-41 can directly regulate germline mRNA via specific sequence elements, identifying potential targets and validating one known target involved in chromatin modification.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Endocrinology & Metabolism
Petrina Koumarianou, Celia Fernandez-Mendez, Danae Fajardo-Delgado, Lidia Mirella Mielu, Pilar Santisteban, Antonio De la Vieja
Summary: This study reveals the involvement of the clathrin-coated machinery in basolateral trafficking of NIS (sodium/iodide symporter), providing further understanding of NIS subcellular sorting mechanisms. These findings will help uncover new targets in thyroid cancer treatment.
Article
Biochemistry & Molecular Biology
Yunan C. Januario, Jessica Eden, Luan S. de Oliveira, Raffaella De Pace, Lucas A. Tavares, Mara E. da Silva-Januario, Vinicius B. Apolloni, Elise L. Wilby, Randolf Altmeyer, Patricia Burgos, Sonia A. L. Correa, David C. Gershlick, Luis L. P. daSilva
Summary: One of the causes of Alzheimer's disease is the increased levels of C99, but the reasons behind this increase are not well understood. This study discovered that the amyloid precursor protein interacts with the clathrin vesicle adaptor AP-1, and this interaction plays a crucial role in the transport and processing of APP. Disrupting the AP-1-mediated transport of APP leads to abnormal processing and degradation, ultimately resulting in increased C99 production and A beta release.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Rhiannon Morris, Yaoyuan Zhang, Julia Ellyard, Carola G. Vinuesa, James M. Murphy, Artem Laktyushin, Nadia J. Kershaw, Jeffrey J. Babon
Summary: The researchers presented structures of the substrate recognition (SH2) domain of LNK in complex with phosphorylated motifs from JAK2 and EPOR, providing insight into its binding specificity and mode of action.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Anle Ge, Zhidian Diao, Fengyi Liu, Yuandong Li, Chaojie Cui, Shoujie Li, Yang Liu, Jian Xu, Bo Ma
Summary: In this study, a low-cost and label-free droplet-based microfluidic platform was developed for rapid microbial growth phenotype screening. The platform allows automated counting and sorting of bacteria in droplets.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Anika Koetemann, Bernd Wollscheid
Summary: This article discusses the importance of apicobasal polarity and surfaceomes in epithelial cells, and identifies potential key regulators of these processes. By using chemoproteomic surfaceome scanning, proteotype maps were established and quantitative distributions of proteins that affect polarized cell functionality were revealed. The study also found that the tumor suppressor PTEN regulates the architecture of the polarized surfaceome and may play a role in collective cell migration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Richard A. Hartz, Vijay T. Ahuja, Susheel J. Nara, C. M. Vijaya Kumar, Raju K. V. L. P. Manepalli, Sarat Kumar Sarvasiddhi, Swarnamba Honkhambe, Vidya Patankar, Bireshwar Dasgupta, Ramkumar Rajamani, Jodi K. Muckelbauer, Daniel M. Camac, Kaushik Ghosh, Matthew Pokross, Susan E. Kiefer, Jeffrey M. Brown, Lisa Hunihan, Michael Gulianello, Martin Lewis, Jonathan S. Lippy, Neha Surti, Brian D. Hamman, Jason Allen, Walter A. Kostich, Joanne J. Bronson, John E. Macor, Carolyn D. Dzierba
Summary: Adaptor protein 2-associated kinase 1 (AAK1) is a potential therapeutic target for neuropathic pain. Inhibition of AAK1 in the spinal cord can achieve an antinociceptive effect. We synthesized a series of AAK1 inhibitors with improved activity by modifying the structure of compound 7.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Cell Biology
Rafael Mattera, Raffaella De Pace, Juan S. Bonifacino
Summary: Adaptor protein 4 (AP-4) is a complex that binds with AAGAB to promote its assembly and plays a role in the development of the central nervous system.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Cell Biology
Mara C. Duncan
Summary: The clathrin adaptor protein complex-1 (AP-1) plays a crucial role in cell physiology and human health by participating in cargo transport and its association with plasma membrane proteins, as well as its involvement in human diseases.
CURRENT OPINION IN CELL BIOLOGY
(2022)
Article
Immunology
Christopher D. Lucas, Christopher B. Medina, Finnius A. Bruton, David A. Dorward, Michael H. Raymond, Turan Tufan, J. Iker Etchegaray, Brady Barron, Magdalena E. M. Oremek, Sanja Arandjelovic, Emily Farber, Suna Onngut-Gumuscu, Eugene Ke, Moira K. B. Whyte, Adriano G. Rossi, Kodi S. Ravichandran
Summary: Panx1, activated by caspases in dying cells, drives efficient epithelial regeneration after tissue injury by regulating injury-induced epithelial proliferation. The release of factors like ATP from dying epithelial cells regulates macrophage phenotype after injury, inducing a reparative response in tissue macrophages that includes promoting injury-induced epithelial proliferation. Panx1-mediated communication between epithelial cells and macrophages is a key step in promoting epithelial regeneration after injury, conserved between mouse lung and zebrafish tailfin.
SCIENCE IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Nairi Hartooni, Jongmin Sung, Ankur Jain, David O. Morgan
Summary: Robust regulatory signals in the cell depend on interactions between short linear motifs and globular proteins. However, studying these interactions is challenging due to limitations in protein production. To address this, the researchers developed a single-molecule off-rate assay to study substrate binding in the APC/C. They found that the substrate's short linear motifs have distinct affinities and specificities for the substrate-binding subunits, and multiple motifs can generate a high-affinity multivalent interaction. These findings reveal the adaptable substrate-binding mechanisms of the APC/C in governing substrate destruction during mitosis.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Miguel Ramirez Moreno, Katy Boswell, Helen L. Casbolt, Natalia A. Bulgakova
Summary: Intracellular trafficking, regulated by the AP-1 complex, plays a crucial role in epithelial morphogenesis and cell adhesion. Knockdown of AP-1 leads to tissue folding and cell death, potentially acting as a tumor suppressor. Furthermore, E-cadherin hyperinternalization and increased expression contribute to cell-cell adhesion maintenance.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Endocrinology & Metabolism
Ariel Galindo-Albarran, Sarah Castan, Jeremy C. Santamaria, Olivier P. Joffre, Bart Haegeman, Paola Romagnoli, Joost P. M. van Meerwijk
Summary: The antigen specificity of Treg is crucial in their in vivo activity. Comparative analysis between NOD and C57BL/6 mice showed comparable diversity of TCRs, suggesting genetic defects affecting the TCR diversity expressed by newly developed Treg may not be involved in the etiology of type 1 diabetes in NOD mice.
Article
Chemistry, Medicinal
Richard A. Hartz, Vijay T. Ahuja, Susheel J. Nara, C. M. Vijaya Kumar, Jeffrey M. Brown, Linda J. Bristow, Ramkumar Rajamani, Jodi K. Muckelbauer, Daniel Camac, Susan E. Kiefer, Lisa Hunihan, Michael Gulianello, Martin Lewis, Amy Easton, Jonathan S. Lippy, Neha Surti, Sreenivasulu N. Pattipati, Manoj Dokania, Saravanan Elavazhagan, Kumaran Dandapani, Brian D. Hamman, Jason Allen, Walter Kostich, Joanne J. Bronson, John E. Macor, Carolyn D. Dzierba
Summary: Effective treatment of chronic pain, especially neuropathic pain, remains a significant unmet medical need. A phenotypic screen of mouse gene knockouts identified AAK1 as a potential therapeutic target for neuropathic pain, leading to the discovery of compound 59, which showed efficacy in pain models. This suggests that AAK1 inhibition is a promising approach for the treatment of neuropathic pain.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Sandra G. Williams, Sandra L. Wolin
Summary: Autoimmunity and autoimmune diseases are common but the repertoire of autoantigens is limited, with nucleic acids and their binding proteins being highly enriched. The origin of these complexes dates back to ancient evolution, and there may be a link between autoimmunity to RNPs and molecular mimicry to microbial orthologs.
TRENDS IN MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Claudia Metz, Claudia Oyanadel, Juan Jung, Claudio Retamal, Jorge Cancino, Jonathan Barra, Jaime Venegas, Guangwei Du, Andrea Soza, Alfonso Gonzalez
Summary: The PA/PDE4/PKA pathway triggers EGFR endocytosis through activation of p38 and ERK1/2, expanding the potential for EGFR transmodulation and interference in cancer.
Article
Oncology
Jonathan Barra, Javier Cerda-Infante, Lisette Sandoval, Patricia Gajardo-Meneses, Jenny F. Henriquez, Mariana Labarca, Claudia Metz, Jaime Venegas, Claudio Retamal, Claudia Oyanadel, Jorge Cancino, Andrea Soza, Mauricio A. Cuello, Juan Carlos Roa, Viviana P. Montecinos, Alfonso Gonzalez
Summary: D-Propranolol shows antitumor effects by affecting the distribution of cell surface EGFR and degradation of gain-of-function mutants of p53 in cancer cells, reducing tumor growth and prolonging survival. It is proposed as a novel drug for the treatment of highly aggressive EGFR- and mutp53-expressing tumors, interfering simultaneously with multiple oncogenic pathways contributing to tumor progression and aggressiveness.
Article
Multidisciplinary Sciences
Elodie Mailler, Carlos M. Guardia, Xiaofei Bai, Michal Jarnik, Chad D. Williamson, Yan Li, Nunziata Maio, Andy Golden, Juan S. Bonifacino
Summary: ATG9A is a transmembrane protein involved in lipid mobilization, essential for autophagosome expansion, mitochondrial fatty acid import, and beta-oxidation. Depletion of ATG9A inhibits autophagy, increases lipid droplet size/number, and blocks transfer of fatty acids to mitochondria. ATG9A localizes to vesicular-tubular clusters associated with TMEM41B and plays a critical role in lipid mobilization from lipid droplets to autophagosomes and mitochondria.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Tanveer Ahmad, Detlef Vullhorst, Rituparna Chaudhuri, Carlos M. Guardia, Nisha Chaudhary, Irina Karavanova, Juan S. Bonifacino, Andres Buonanno
Summary: This study investigates the processing and sorting of NRG3 in neurons. It finds that NRG3 is cleaved in the trans-Golgi network and released, and then re-endocytosed and transported into axons. NRG3 accumulates at presynaptic terminals through stable interaction with its receptor. This trans-synaptic retention mechanism may explain the polarized expression of other neuronal transmembrane ligands and receptors.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Tal Keren-Kaplan, Amra Saric, Saikat Ghosh, Chad D. Williamson, Rui Jia, Yan Li, Juan S. Bonifacino
Summary: This study identifies RUFY3 and RUFY4 as ARL8 effectors that promote retrograde transport of endolysosomes along microtubules by interacting with dynein-dynactin. RUFY3 and RUFY4 also play a role in concentration and redistribution of endolysosomes in different cellular contexts. These findings highlight the importance of ARL8 in regulating both anterograde and retrograde transport of endolysosomes.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Gabriela Vargas, Omar Cortes, Eloisa Arias-Munoz, Sergio Hernandez, Cristobal Cerda-Troncoso, Laura Hernandez, Alexis E. Gonzalez, Michael H. Tatham, Hianara A. Bustamante, Claudio Retamal, Jorge Cancino, Manuel Varas-Godoy, Ronald T. Hay, Alejandro Rojas-Fernandez, Viviana A. Cavieres, Patricia V. Burgos
Summary: Macroautophagy and the ubiquitin proteasome system are interconnected in maintaining cellular homeostasis. HERPUD1, a protein regulated by proteasomal degradation, stabilizes ER and lysosomal network and negatively regulates autophagy, promoting cell survival under stress conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Clinical Neurology
Francisca Barake, Marcela Bravo-Zehnder, Alfonso Gonzalez
Summary: This review study explores a new regulation system in the hippocampus involving the neuronal surface P antigen (NSPA) and the tyrosine phosphatase PTPMEG/PTPN4, which has potential therapeutic implications for cognitive dysfunction in systemic lupus erythematosus (SLE) patients.
CURRENT OPINION IN NEUROLOGY
(2022)
Article
Cell Biology
Rafael Mattera, Raffaella De Pace, Juan S. Bonifacino
Summary: Adaptor protein 4 (AP-4) is a complex that binds with AAGAB to promote its assembly and plays a role in the development of the central nervous system.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Cell Biology
Juan S. Bonifacino
Summary: This passage describes the application of electron microscopy in studying eukaryotic cells and the importance of this development in understanding the internal organization and function of these cells. The author's long-term research focuses on protein sorting and organelle transport in the cytoplasm, and has revealed various mechanisms and defects leading to human diseases.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Cell Biology
Ariane Schleinitz, Lara-Alina Poettgen, Tal Keren-Kaplan, Jing Pu, Paul Saftig, Juan S. Bonifacino, Albert Haas, Andreas Jeschke
Summary: This study biochemically characterized endosomes purified from untreated or experimentally manipulated cells to establish the functional interdependence of HOPS and interacting proteins. The results showed that Rab2a and Rab7 are present in late endosomes, while Arl8 and BORC are present in lysosomes, and HOPS drives LE-LYS fusion by bridging late endosomal Rab2a with lysosomal BORC-anchored Arl8. Additionally, Rab7 promotes fusion by moving LEs towards LYSs via dynein.
Article
Cell Biology
Lisette Sandoval, Mariana Labarca, Claudio Retamal, Paula Sanchez, Juan Larrain, Alfonso Gonzalez
Summary: This study reveals the mechanism of how newly synthesized Hedgehog proteins are transported within polarized epithelial cells, from the trans-Golgi network to the cell surface, and then to different domains. This process is regulated by the lipid modifications and mediated by the protein Dispatched-1.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Ganesh Vilas Shelke, Chad D. Williamson, Michal Jarnik, Juan S. Bonifacino
Summary: This study shows that disruption of the BORC-ARL8-HOPS pathway increases exosome secretion by impairing fusion of multivesicular endosomes with lysosomes. This leads to the release of intraluminal vesicles into the extracellular space. Therefore, suppressing the BORC-ARL8-HOPS pathway could be used to enhance exosome yields.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Xin Chen, Thomas Dong, Yuhui Hu, Raffaella De Pace, Rafael Mattera, Kathrin Eberhardt, Marvin Ziegler, Terry Pirovolakis, Mustafa Sahin, Juan S. Bonifacino, Darius Ebrahimi-Fakhari, Steven J. Gray
Summary: Spastic paraplegia 50 (SPG50) is an ultrarare childhood-onset neurological disorder caused by biallelic loss-of-function variants in the AP4M1 gene. In preclinical studies, adeno-associated virus (AAV)/AP4M1 gene therapy showed promising results in rescuing the symptoms of SPG50. The therapy was effective in transducing patient-derived fibroblasts and demonstrated dose-dependent effects in Ap4m1-KO mice, supporting the potential for a clinical trial in treating SPG50 through intrathecal administration of AAV9/AP4M1.
JOURNAL OF CLINICAL INVESTIGATION
(2023)