Article
Multidisciplinary Sciences
Xiaofen Li, Qirui Zhao, Xiaojie Yu, Wenhan Cao, Yingyi Zhang, Wanying Feng, Liwen Jiang, David Z. He, Robert Z. Qi, Pingbo Huang
Summary: In this study, a previously undescribed organelle called apicosome was identified in hair cells of the neonatal mouse inner ear. It has a diameter of approximately 500 nm and shows transient appearance and itinerant nature during cochlear hair cell development. Unlike cochlear hair cells, the apicosome persists in vestibular hair cells even in adults. The timing of apicosome translocation and disappearance in cochlear hair cells during development is correlated with kinocilium development and maintenance. Interestingly, transdifferentiated hair cells from supporting cells also contain apicosome.
Article
Multidisciplinary Sciences
Jie Li, Shuang Liu, Chenmeng Song, Qun Hu, Zhikai Zhao, Tuantuan Deng, Yi Wang, Tong Zhu, Linzhi Zou, Shufeng Wang, Jiaofeng Chen, Lian Liu, Hanqing Hou, Kexin Yuan, Hairong Zheng, Zhiyong Liu, Xiaowei Chen, Wenzhi Sun, Bailong Xiao, Wei Xiong
Summary: The knockout of the mechanosensitive ion channel PIEZO2 in cochlea disrupts ultrasonic hearing in mice, but not low-frequency hearing. Deletion of Piezo2 in outer hair cells specifically abolishes associative learning in mice during hearing exposure at ultrasonic frequencies. The study demonstrates that OHCs serve as effector cells, combining with PIEZO2 as an essential molecule for ultrasonic hearing in mice.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Andre Landin Malt, Shaylyn Clancy, Diane Hwang, Alice Liu, Connor Smith, Margaret Smith, Maya Hatley, Christopher Clemens, Xiaowei Lu
Summary: A non-canonical Wnt signaling pathway has been found to promote cochlear outgrowth and coordinate planar polarization of hair cells. Gsk3 beta and Rac1 play crucial roles in this pathway, as shown in genetic rescue experiments.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Difei Wang, Jun Zhou
Summary: Primary cilia are highly specialized organelles protruding from cell membranes, and mutations in genes encoding ciliary proteins can cause ciliopathies; this paper reviews the structure and function of specialized primary cilia, known as kinocilia, in the mammalian auditory system, and discusses potential therapeutic approaches for auditory ciliopathies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xiaolong Fu, Peifeng Wan, Ling Lu, Yingcui Wan, Ziyi Liu, Guodong Hong, Shengda Cao, Xiuli Bi, Jing Zhou, Ruifeng Qiao, Siwei Guo, Yu Xiao, Bingzheng Wang, Miao Chang, Wen Li, Peipei Li, Aizhen Zhang, Jin Sun, Renjie Chai, Jiangang Gao
Summary: In this study, two peroxisome-deficient mouse models were established to investigate the role of peroxisomes in hearing. The results showed that peroxisomes mainly function in the hair cells of cochleae and regulate BK channels, which are essential for auditory function. These findings suggest that BK channels could be potential targets for treating peroxisome-related hearing diseases.
Article
Genetics & Heredity
Shasha Zhang, Ying Dong, Ruiying Qiang, Yuan Zhang, Xiaoli Zhang, Yin Chen, Pei Jiang, Xiangyu Ma, Leilei Wu, Jingru Ai, Xia Gao, Pengjun Wang, Jie Chen, Renjie Chai
Summary: Strip1 is a core component of the STRIPAK complex and its knockout in mice is embryonic lethal, indicating its importance in cochlear hair cell development and maturation. Studies on Strip1 expression and function in the mammalian cochlea are still needed for further understanding its roles.
FRONTIERS IN GENETICS
(2021)
Article
Neurosciences
Alix Trouillet, Katharine K. Miller, Shefin Sam George, Pei Wang, Noor-E-Seher Ali, Anthony Ricci, Nicolas Grillet
Summary: The study identified the importance of the Loxhd1/DFNB77 gene in the mechanotransduction process in cochlear hair cells. Mutations in Loxhd1 in mice resulted in mechanotransduction defects without morphological defects or reduction in tip link number. These findings revealed a novel LOXHD1-dependent step in hair bundle development critical for mechanotransduction in mature hair cells and normal hearing function in mice and humans.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Cell Biology
Yuhua Zhang, Qiaojun Fang, Hongfeng Wang, Jieyu Qi, Shan Sun, Menghui Liao, Yunhao Wu, Yangnan Hu, Pei Jiang, Cheng Cheng, Xiaoyun Qian, Mingliang Tang, Wei Cao, Shang Xiang, Chen Zhang, Jianming Yang, Xia Gao, Zheng Ying, Renjie Chai
Summary: This study found that aminoglycosides cause hair cell injury by impairing mitophagy, a process that degrades damaged mitochondria. The impaired mitophagy is characterized by blocked recruitment of PRKN to mitochondria and phagophore recognition of damaged mitochondria. Additionally, neomycin disrupts mitophagy by inhibiting Pink1 expression and inducing ATF3 expression. Treatment with a mitophagy activator can rescue neomycin-treated hair cells by increasing mitophagy. Overall, this study suggests that modulating or intervening in mitophagy may have therapeutic potential for aminoglycoside-induced hearing loss.
Article
Chemistry, Physical
Mariana Kober, Silvia Illa-Tuset, Lidia Ferrer-Tasies, Evelyn Moreno-Calvo, Witold I. Tatkiewicz, Natascia Grimaldi, David Pina, Alejandro Perez Perez, Vega Lloveras, Jose Vidal-Gancedo, Donatella Bulone, Imma Ratera, Jan Skov Pedersen, Dganit Danino, Jaume Veciana, Jordi Faraudo, Nora Ventosa
Summary: This study investigates the stability and formation mechanism of CHOL/CTAB quatsomes, which are nanovesicles formed through the self-assembly of cholesterol and cetyltrimethylammonium bromide in water. Experimental and simulation results show that CHOL/CTAB quatsomes are thermodynamically stable nanovesicles, but they do not exhibit the classical membrane curvature induced by composition asymmetry.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Zhen Liu, Peng Chen, Yuan-Yuan Li, Meng-Wen Li, Qi Liu, Wen-Lu Pan, Dong-Ming Xu, Jing Bai, Li-Biao Zhang, Jie Tang, Peng Shi
Summary: The cochlear hair cells of echolocating bats show resistance to intense noise, preventing noise-induced hearing loss. Experimental evidence indicates that echolocating bats can maintain their hearing and cochlear health even after exposure to continuous intense noise.
JOURNAL OF GENETICS AND GENOMICS
(2021)
Article
Cell Biology
Keji Yan, Wen Zong, Haibo Du, Xiaoyan Zhai, Rui Ren, Shuang Liu, Wei Xiong, Yanfei Wang, Zhigang Xu
Summary: In this study, it was found that BAIAP2L2 is localized at the tips of inner ear hair cells' stereocilia, and its inactivation leads to degeneration of mechanotransducing stereocilia, resulting in hearing loss. BAIAP2L2 binds to components of the row 2 complex and MET complex, indicating its crucial role in maintaining stereocilia.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Acoustics
Renata Sisto, Arturo Moleti
Summary: The low-pass characteristic of OHC voltage response to mechanical stimulation may pose challenges for cochlear models, but evidence suggests that the local cutoff frequency changes approaching the cochlear base. By incorporating low-pass filtering for an internal force term, models can better simulate the frequency dependence of different regions of the cochlea.
JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA
(2021)
Article
Neurosciences
Qiongmin Zhang, Zhiqun Yao, Fang Chen, Xue Wang, Man Wang, Junze Lu, Yu Meng, Lei Xu, Yuechen Han, Wenwen Liu, Haibo Wang
Summary: This study found that teicoplanin can cause death of cochlear hair cells and decrease the expression of TIGAR. Overexpression of TIGAR can reduce cell death and apoptosis, and decrease the levels of reactive oxygen species induced by teicoplanin. Antioxidant therapy can prevent cell death caused by teicoplanin.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Neurosciences
Marco Manca, Piece Yen, Paolo Spaiardi, Giancarlo Russo, Roberta Giunta, Stuart L. Johnson, Walter Marcotti, Sergio Masetto
Summary: The residual I-Ca in both auditory and vestibular hair cells from Ca(V)1.3(-/-) mice was significantly reduced, but the K+ current profile changes in vestibular hair cells suggest that non-exocytotic transmission remains functional.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Cell Biology
Shahar Taiber, Oren Gozlan, Roie Cohen, Leonardo R. Andrade, Ellen F. Gregory, Daniel A. Starr, Yehu Moran, Rebecca Hipp, Matthew W. Kelley, Uri Manor, David Sprinzak, Karen B. Avraham
Summary: Nuclear positioning is crucial for cell functionality, and Nesprin proteins are involved in nuclear positioning in various cell types. In outer hair cells (OHCs), Nesprin-4 plays an important role in nuclear positioning and hearing. This study found that the interaction between Nesprin-4 and the microtubule motor kinesin-1 is mediated by a conserved 4 amino-acid motif. The mislocalization and death of OHC nuclei coincide with the onset of hearing and electromotility, and are restricted to outer hair cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kimberly Siletti, Basile Tarchini, A. J. Hudspeth
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Biographical-Item
Developmental Biology
Michel Cayouette
Article
Developmental Biology
Basile Tarchini, Chantal Longo-Guess, Cong Tian, Abigail L. D. Tadenev, Nicholas Devanney, Kenneth R. Johnson
DEVELOPMENTAL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Pierre Mattar, Milanka Stevanovic, Ivana Nad, Michel Cayouette
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Abigail L. D. Tadenev, Anil Akturk, Nicholas Devanney, Pranav Dinesh Mathur, Anna M. Clark, Jun Yang, Basile Tarchini
Article
Multidisciplinary Sciences
Philippe Jean, Oezge Demet Oezcete, Basile Tarchini, Tobias Moser
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Review
Neurosciences
Basile Tarchini, Xiaowei Lu
NEUROSCIENCE LETTERS
(2019)
Article
Developmental Biology
Awais Javed, Pierre Mattar, Suying Lu, Kamil Kruczek, Magdalena Kloc, Anai Gonzalez-Cordero, Rod Bremner, Robin R. Ali, Michel Cayouette
Article
Developmental Biology
Amandine Jarysta, Basile Tarchini
Summary: The MPDZ protein plays a crucial role in the morphogenesis of apical hair cells in mice. It is enriched at the apical membrane of hair cells, along with other proteins, to maintain proper segregation of apical blueprint proteins, including GNAI-GPSM2. Loss of this blueprint results in misaligned stereocilia placement and permanently misshapen hair bundles in Mpdz mutant hair cells.
Article
Multidisciplinary Sciences
Anil Akturk, Matthew Day, Basile Tarchini
Summary: In this study, the researchers discovered the molecular mechanism by which GPSM2-GNAI complex is formed and regulated by RGS12. RGS12 and DAPLE are asymmetrically distributed at the hair cell apical junction, organizing mechanosensory stereocilia in rows of graded heights. GPSM2 and RGS12 share GoLoco motifs and compete for binding to GNAI. The polarized GEF/GAP junctional activity dissociates heterotrimeric G proteins, generating free GNAI(GDP) for GPSM2 at the adjacent apical membrane, which imparts asymmetry to the forming stereocilia and enables sensory function in hair cells.
Article
Multidisciplinary Sciences
Marine Lacomme, Sarah C. Hales, Thomas W. Brown, Katarina Stevanovic, Christine Jolicoeur, Jenny Cai, Therence Bois, Melissa Desrosiers, Deniz Dalkara, Michel Cayouette
Summary: This study reveals Numb as a key regulator of intracellular Tau levels and identifies Numb-72 as a potential therapeutic factor for tauopathies.
Article
Developmental Biology
Awais Javed, Pedro L. Santos-Franca, Pierre Mattar, Allie Cui, Fatima Kassem, Michel Cayouette
Summary: Temporal identity factors, such as Ikaros zinc-finger transcription factors Ikzf1 and Ikzf4, play important roles in regulating the production of specific cell types during neural development. Ikzf4 acts redundantly with Ikzf1 during early retinal development to promote cone photoreceptor production, while it is involved in gliogenesis and Müller glia production in the late stages. These findings highlight the combinatorial role of Ikaros family members in neural development and provide insights into the temporal regulation of cell fate output.
Article
Multidisciplinary Sciences
Camille Boudreau-Pinsonneault, Luke Ajay David, Jose Alex Lourenco Fernandes Fernandes, Awais Javed, Michel Fries, Pierre Mattar, Michel Cayouette
Summary: This study demonstrates that coexpression of early temporal identity transcription factors Ikzf1 and Ikzf4 can directly convert mouse retinal glial cells into photoreceptor cells and reprogram mouse embryonic fibroblasts into neurons. These findings uncover the potential of temporal identity factors in reprogramming terminally differentiated cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Shihai Jia, Evan M. Ratzan, Ellison J. Goodrich, Raisa Abrar, Luke Heiland, Basile Tarchini, Michael R. Deans
Summary: The vestibular maculae in the inner ear play a crucial role in detecting acceleration and coordinating balance. The transcription factor EMX2 is responsible for establishing the planar polarized organization in hair cells, and we have discovered that it negatively regulates the serine threonine kinase STK32A. STK32A is involved in aligning the polarity of hair cell bundles and reinforcing the formation of LPR.
Article
Cell Biology
Michel Fries, Thomas W. Brown, Christine Jolicoeur, Benoit Boulan, Camille Boudreau-Pinsonneault, Awais Javed, Penelope Abram, Michel Cayouette
Summary: POU3F1 is expressed in contralateral retinal ganglion cells (cRGCs) but not in ipsilateral RGCs (iRGCs) in mice. Inactivation of Pou3f1 reduces the proportion of cRGCs and increases the proportion of iRGCs, leading to abnormal projection ratios at the optic chiasm. This study demonstrates that POU3F1 regulates the gene expression network of cRGCs and has the potential for optic nerve regenerative therapies.