Article
Biology
Jake Leyhr, Laura Waldmann, Beata Filipek-Gorniok, Hanqing Zhang, Amin Allalou, Tatjana Haitina
Summary: This study identifies a conserved Nkx3.2 enhancer element in gnathostomes and demonstrates its activity in the jaw joint of zebrafish. The enhancer shows functional conservation in different gnathostomes, indicating its importance in jaw development. Deletion of the enhancer leads to jaw joint deformations and fusion in zebrafish.
Article
Genetics & Heredity
Ioanna Pavlaki, Michael Shapiro, Giuseppina J. Pisignano, Stephanie M. E. R. Jones, Jelena W. Telenius, Silvia M. Munoz-Descalzo, Robert Williams, Jim M. Hughes, Keith Vance
Summary: Central nervous system-expressed long non-coding RNAs (lncRNAs) are often located near protein coding genes involved in transcriptional control. The study identified the cis-regulatory interactions controlling the co-expression of the Paupar-Pax6 lncRNA-mRNA locus in the brain. The TCF7L2 transcription factor binds to specific cis-regulatory elements to regulate the expression of Paupar and Pax6 together.
Article
Developmental Biology
Fuyun Bian, Marwa Daghsni, Fangfang Lu, Silvia Liu, Jeffrey M. Gross, Issam Aldiri
Summary: The transcription factor Vsx2 plays a critical role in retinal proliferation and bipolar cell differentiation. It binds to and transactivates its enhancer in association with Pax6. Vsx2 deletion in mice results in specific abnormalities in retinal proliferation and bipolar cell differentiation. Vsx2 occupies cis-regulatory elements nearby genes associated with photoreceptor differentiation and homeostasis in both mouse and human retina. It can also suppress Otx2-dependent activation of the Prdm1 enhancer. These findings provide important insights into the mechanisms of Vsx2 engagement with gene regulatory networks in retinal development.
Article
Biotechnology & Applied Microbiology
Kurtis Stefan, Artem Barski
Summary: Cis-regulatory elements (CRE) are essential for coordinating gene expression programs in specific cells. The STARR-Seq technique has been used to annotate functional CREs, but is mostly limited to cell lines. In this study, Lenti-STARR-Seq was successfully implemented in human CD4+ T cells, providing a genome-wide analysis of CRE function in primary cells. The study identified thousands of enhancers and negative regulatory elements (NREs) in CD4+ T cells, and revealed differences in nucleosome organization between enhancers and NREs. The findings also support the concept of silencer repurposing as enhancers in alternate cell types.
Article
Biology
Diana Avalos, Guillaume Rey, Diogo M. Ribeiro, Anna Ramisch, Emmanouil T. Dermitzakis, Olivier Delaneau
Summary: An analysis of cis-regulatory domains (CRDs) derived from ChIP-seq peaks and methylation data reveals cell-type specific regulatory mechanisms in immunity. Important biological mechanisms, such as cell-specific transcription factor binding sites and immune disease-associated loci, are discovered through this study. CRD-QTLs aid in interpreting GWAS findings and mapping functional regulatory units using population genomics allows the discovery of important mechanisms in gene expression regulation in immune cells. This study establishes a comprehensive resource for understanding cell-type specific regulatory mechanisms of immunity.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Damien J. Downes, Robert A. Beagrie, Matthew E. Gosden, Jelena Telenius, Stephanie J. Carpenter, Lea Nussbaum, Sara De Ornellas, Martin Sergeant, Chris Q. Eijsbouts, Ron Schwessinger, Jon Kerry, Nigel Roberts, Arun Shivalingam, Afaf El-Sagheer, A. Marieke Oudelaar, Tom Brown, Veronica J. Buckle, James O. J. Davies, Jim R. Hughes
Summary: This study demonstrates the Nuclear-Titrated Capture-C method for generating high-resolution genome-wide interaction profiles, overcoming limitations of current 3C methods.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Seungsoo Kim, Joanna Wysocka
Summary: Uncovering the cis-regulatory code that governs gene transcription in a given genome and cellular state remains a central goal of biology. This review discusses the different layers of regulation that influence transcriptional outputs, including the binding of transcription factors to specific DNA sequences, the role of cofactors in facilitating their function, and the complex interplay within regulatory landscapes. The authors propose that a quantitative, mechanistically informed model of transcriptional regulation is essential for ultimately understanding the cis-regulatory code.
Article
Multidisciplinary Sciences
Ioannis Sarropoulos, Mari Sepp, Robert Fromel, Kevin Leiss, Nils Trost, Evgeny Leushkin, Konstantin Okonechnikov, Piyush Joshi, Peter Giere, Lena M. Kutscher, Margarida Cardoso-Moreira, Stefan M. Pfister, Henrik Kaessmann
Summary: This study investigated the regulatory basis of mouse cerebellum development from early neurogenesis to adulthood using snATAC-seq profiles. The research revealed spatiotemporal heterogeneity among progenitor cells and gradual divergence in the regulatory programs of cerebellar neurons during differentiation. Comparison to vertebrate genomes showed a shared decrease in CRE conservation during development and differentiation, as well as differences in constraint between cell types. The study provides insights into mammalian organ development dynamics and evolutionary gene regulation in cerebellar cells.
Article
Biochemistry & Molecular Biology
Anushua Biswas, Leelavati Narlikar
Summary: cisDIVERSITY is a new statistical framework that analyzes various gene regulation activities by modeling regions as diverse modules characterized by combinations of motifs while simultaneously learning the motifs themselves.
Article
Multidisciplinary Sciences
Anat Kreimer, Tal Ashuach, Fumitaka Inoue, Alex Khodaverdian, Chengyu Deng, Nir Yosef, Nadav Ahituv
Summary: This study systematically characterizes the role of regulatory elements and motifs in guiding cellular differentiation. The researchers find that the sequence of DNA binding motifs determines the activity direction, while the magnitude of effect depends on the cellular environment. They also observe that fine-tuning transcription rates is often achieved through the combined activity of activating and repressing elements. This work provides insights into the components needed to induce different transcriptional patterns during neural differentiation.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Rebekah R. Starks, Haninder Kaur, Geetu Tuteja
Summary: The placenta is a temporary organ that provides nutrients, oxygen, and protection to the developing fetus in utero. Defects in its development may lead to severe consequences for both the mother and fetus due to misregulated gene expression. Analysis of ChIP-seq data for histone modifications in the mouse placenta identified associations of active and poised promoters and enhancers with different categories of genes, providing a foundation for future studies on gene regulation in the midgestation mouse placenta.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Jaya Krishnan, Chris W. Seidel, Ning Zhang, Narendra Pratap Singh, Jake VanCampen, Robert Peuss, Shaolei Xiong, Alexander Kenzior, Hua Li, Joan W. Conaway, Nicolas Rohner
Summary: Cis-regulatory changes have been found to contribute to the metabolic adaptation in different morphotypes of Astyanax mexicanus, with genetically diverged and differentially regulated elements identified between surface and cave populations. This study sheds light on the molecular mechanisms underlying metabolic adaptations to nutrient-deprived cave environments.
Article
Biology
Pengyu Ni, Joshua Moe, Zhengchang Su
Summary: This study proposes a two-step strategy to accurately predict the distribution of CRMs in the genome and their functional states in various cell/tissue types by integrating ChIP-seq data and using machine learning methods. The results show that functional states of CRMs can be accurately predicted using only 1 to 4 epigenetic marks, and the approach is more cost-effective than existing methods. The study also reveals common epigenetic rules for defining functional states of CRMs in humans and mice.
Article
Genetics & Heredity
Damir Baranasic, Matthias Hortenhuber, Piotr J. Balwierz, Tobias Zehnder, Abdul Kadir Mukarram, Chirag Nepal, Csilla Varnai, Yavor Hadzhiev, Ada Jimenez-Gonzalez, Nan Li, Joseph Wragg, Fabio M. D'Orazio, Dorde Relic, Mikhail Pachkov, Noelia Diaz, Benjamin Hernandez-Rodriguez, Zelin Chen, Marcus Stoiber, Michael Dong, Irene Stevens, Samuel E. Ross, Anne Eagle, Ryan Martin, Oluwapelumi Obasaju, Sepand Rastegar, Alison C. McGarvey, Wolfgang Kopp, Emily Chambers, Dennis Wang, Hyejeong R. Kim, Rafael D. Acemel, Silvia Naranjo, Maciej Lapinski, Vanessa Chong, Sinnakaruppan Mathavan, Bernard Peers, Tatjana Sauka-Spengler, Martin Vingron, Piero Carninci, Uwe Ohler, Scott Allen Lacadie, Shawn M. Burgess, Cecilia Winata, Freek van Eeden, Juan M. Vaquerizas, Jose Luis Gomez-Skarmeta, Daria Onichtchouk, Ben James Brown, Ozren Bogdanovic, Erik van Nimwegen, Monte Westerfield, Fiona C. Wardle, Carsten O. Daub, Boris Lenhard, Ferenc Muller
Summary: Zebrafish, an important model organism for studying embryonic development and human diseases, lacks a systematic functional annotation program. The international DANIO-CODE consortium addressed this issue by creating a central repository to store and process zebrafish developmental functional genomic data. They improved existing annotations and identified over 140,000 cis-regulatory elements throughout development. They also compared regulatory elements and epigenomic landscapes between zebrafish and mouse, predicting functional relationships between them. This study extends the utility of zebrafish developmental genomics to mammals.
Review
Biochemical Research Methods
Peter A. Crisp, Pooja Bhatnagar-Mathur, Penny Hundleby, Ian D. Godwin, Peter M. Waterhouse, Lee T. Hickey
Summary: Traditional plant breeding has achieved certain success, but it is facing the challenge of meeting the growing demand. To fill the yield gaps and meet consumer preferences, new breeding strategies and sources of genetic variation are needed, and it is important to go beyond the traditional gene-centric thinking and consider the roles of epigenetic variation and cis-regulatory variation in plant traits.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Pediatrics
Neha S. Bhatia, Jiin Ying Lim, Carine Bonnard, Jyn-Ling Kuan, Maggie Brett, Heming Wei, Breana Cham, Huilin Chin, Celia Bosso-Lefevre, Perumal Dharuman, Nathalie Escande-Beillard, Arun George Devasia, Chew Yin Jasmine Goh, Sylvia Kam, Wendy Kein-Meng Liew, Woei Kang Liew, Grace Lin, Kanika Jain, Alvin Yu-Jin Ng, Deepa Subramanian, Min Xie, Yuen-Ming Tan, Nilesh R. Tawari, Zenia Tiang, Teck Wah Ting, Sumanty Tohari, Cheuk Ka Tong, Alexander Lezhava, Sarah B. Ng, Hai Yang Law, Byrappa Venkatesh, Swati Tomar, Raman Sethi, Grace Tan, Arthi Shanmugasundaram, Denise Li-Meng Goh, Poh San Lai, Angeline Lai, Ee Shien Tan, Ivy Ng, Bruno Reversades, Ene Choo Tan, Roger Foo, Saumya Shekhar Jamuar
Summary: Using NGS technology, particularly WES or WGS, can improve diagnostic yield in patients with suspected genetic disorders in the Asian setting. Trio sequencing shows higher diagnostic yield for certain phenotypes, and positive results can lead to changes in patient treatment.
ARCHIVES OF DISEASE IN CHILDHOOD
(2021)
Letter
Dermatology
A. Y. T. Loh, C. M. Ho, S. Muthiah, B. Venkatesh, S. Zwolinski, A. P. J. J. Bray, B. Reversade, N. Rajan, T. J. Carney
BRITISH JOURNAL OF DERMATOLOGY
(2021)
Article
Multidisciplinary Sciences
Orlando B. Giorgetti, Prashant Shingate, Connor P. O'Meara, Vydianathan Ravi, Nisha E. Pillai, Boon-Hui Tay, Aravind Prasad, Norimasa Iwanami, Heok Hui Tan, Michael Schorpp, Byrappa Venkatesh, Thomas Boehm
Summary: The structure of antigen receptor repertoires in vertebrates exhibits self-similarity regardless of body size, with minifish achieving immunocompetence through robust scale-free networks composed of thousands of lymphocytes. This ensures immune reactivity even when cells are lost or clone sizes fluctuate during immune responses.
Article
Ecology
Alex de Mendoza, Daniel Poppe, Sam Buckberry, Jahnvi Pflueger, Caroline B. Albertin, Tasman Daish, Stephanie Bertrand, Elisa de la Calle-mustienes, Jose Luis Gomez-Skarmeta, Joseph R. Nery, Joseph R. Ecker, Boris Baer, Clifton W. Ragsdale, Frank Grutzner, Hector Escriva, Byrappa Venkatesh, Ozren Bogdanovic, Ryan Lister
Summary: The study reveals that non-CpG DNA methylation is restricted to vertebrates, particularly enriched within a highly conserved set of developmental genes in mammalian brains, indicating a deeply conserved regulatory program. These findings suggest that the emergence of non-CpG methylation may have fostered the evolution of sophisticated cognitive abilities found in the vertebrate lineage.
NATURE ECOLOGY & EVOLUTION
(2021)
Article
Multidisciplinary Sciences
Chunyan Li, Melisa Olave, Yali Hou, Geng Qin, Ralf F. Schneider, Zexia Gao, Xiaolong Tu, Xin Wang, Furong Qi, Alexander Nater, Andreas F. Kautt, Shiming Wan, Yanhong Zhang, Yali Liu, Huixian Zhang, Bo Zhang, Hao Zhang, Meng Qu, Shuaishuai Liu, Zeyu Chen, Jia Zhong, He Zhang, Lingfeng Meng, Kai Wang, Jianping Yin, Liangmin Huang, Byrappa Venkatesh, Axel Meyer, Xuemei Lu, Qiang Lin
Summary: Seahorses have a global distribution in tropical to temperate coastal waters and show many adaptations for a sedentary, cryptic lifestyle. New genome assembly and re-sequenced genomes of 21 other species shed light on the evolutionary origin and global dispersal routes of seahorses, revealing that rafting via ocean currents compensates for poor dispersal and bony spines likely evolved multiple times through independent substitutions in a key developmental gene.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Arang Rhie, Shane A. McCarthy, Olivier Fedrigo, Joana Damas, Giulio Formenti, Sergey Koren, Marcela Uliano-Silva, William Chow, Arkarachai Fungtammasan, Juwan Kim, Chul Lee, Byung June Ko, Mark Chaisson, Gregory L. Gedman, Lindsey J. Cantin, Francoise Thibaud-Nissen, Leanne Haggerty, Iliana Bista, Michelle Smith, Bettina Haase, Jacquelyn Mountcastle, Sylke Winkler, Sadye Paez, Jason Howard, Sonja C. Vernes, Tanya M. Lama, Frank Grutzner, Wesley C. Warren, Christopher N. Balakrishnan, Dave Burt, Julia M. George, Matthew T. Biegler, David Iorns, Andrew Digby, Daryl Eason, Bruce Robertson, Taylor Edwards, Mark Wilkinson, George Turner, Axel Meyer, Andreas F. Kautt, Paolo Franchini, H. William Detrich, Hannes Svardal, Maximilian Wagner, Gavin J. P. Naylor, Martin Pippel, Milan Malinsky, Mark Mooney, Maria Simbirsky, Brett T. Hannigan, Trevor Pesout, Marlys Houck, Ann Misuraca, Sarah B. Kingan, Richard Hall, Zev Kronenberg, Ivan Sovi, Christopher Dunn, Zemin Ning, Alex Hastie, Joyce Lee, Siddarth Selvaraj, Richard E. Green, Nicholas H. Putnam, Ivo Gut, Jay Ghurye, Erik Garrison, Ying Sims, Joanna Collins, Sarah Pelan, James Torrance, Alan Tracey, Jonathan Wood, Robel E. Dagnew, Dengfeng Guan, Sarah E. London, David F. Clayton, Claudio Mello, Samantha R. Friedrich, Peter Lovell, Ekaterina Osipova, Farooq O. Al-Ajli, Simona Secomandi, Heebal Kim, Constantina Theofanopoulou, Michael Hiller, Yang Zhou, Robert S. Harris, Kateryna D. Makova, Paul Medvedev, Jinna Hoffman, Patrick Masterson, Karen Clark, Fergal Martin, Kevin Howe, Paul Flicek, Brian P. Walenz, Woori Kwak, Hiram Clawson, Mark Diekhans, Luis Nassar, Benedict Paten, Robert H. S. Kraus, Andrew J. Crawford, M. Thomas P. Gilbert, Guojie Zhang, Byrappa Venkatesh, Robert W. Murphy, Klaus-Peter Koepfli, Beth Shapiro, Warren E. Johnson, Federica Di Palma, Tomas Marques-Bonet, Emma C. Teeling, Tandy Warnow, Jennifer Marshall Graves, Oliver A. Ryder, David Haussler, Stephen J. O'Brien, Jonas Korlach, Harris A. Lewin, Kerstin Howe, Eugene W. Myers, Richard Durbin, Adam M. Phillippy, Erich D. Jarvis
Summary: The Vertebrate Genome Project and the international Genome 10K consortium have collaborated to generate high-quality genome assemblies for 16 species representing six major vertebrate lineages, leading to new biological discoveries. Long-read sequencing technologies are essential for maximizing genome quality, and addressing complex repeats and haplotype heterozygosity are crucial for reducing assembly errors and improving completeness of reference genomes. The lessons learned from this project have paved the way for the Vertebrate Genomes Project (VGP), an international effort to generate high-quality, complete reference genomes for all known vertebrate species.
Article
Multidisciplinary Sciences
Yoichiro Nakatani, Prashant Shingate, Vydianathan Ravi, Nisha E. Pillai, Aravind Prasad, Aoife McLysaght, Byrappa Venkatesh
Summary: The study using the probabilistic macrosynteny model to reconstruct ancestral genomes revealed important findings about the early evolutionary history of vertebrates: the polyploidization events that occurred after the divergence of cyclostomes and gnathostomes, and the gnathostome-specific tetraploidization event.
NATURE COMMUNICATIONS
(2021)
Correction
Multidisciplinary Sciences
Yoichiro Nakatani, Prashant Shingate, Vydianathan Ravi, Nisha E. Pillai, Aravind Prasad, Aoife McLysaght, Byrappa Venkatesh
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Meng Qu, Yali Liu, Yanhong Zhang, Shiming Wan, Vydianathan Ravi, Geng Qin, Han Jiang, Xin Wang, Huixian Zhang, Bo Zhang, Zexia Gao, Ann Huysseune, Zhixin Zhang, Hao Zhang, Zelin Chen, Haiyan Yu, Yongli Wu, Lu Tang, Chunyan Li, Jia Zhong, Liming Ma, Fengling Wang, Hongkun Zheng, Jianping Yin, Paul Eckhard Witten, Axel Meyer, Byrappa Venkatesh, Qiang Lin
Summary: The study reveals that the iconic features of seadragons have evolved through co-option of genes, including leaf-like appendages, a toothless tubular mouth, and male pregnancy involving incubation of fertilized eggs. Additionally, the loss of a specific gene, scpp5, may contribute to the tooth loss in syngnathids, and a putative sex-determining locus encoding a male-specific gene shared by common seadragon and alligator pipefish has been identified.
Article
Genetics & Heredity
Emmanuelle Szenker-Ravi, Tim Ott, Muznah Khatoo, Anne Moreau de Bellaing, Wei Xuan Goh, Yan Ling Chong, Anja Beckers, Darshini Kannesan, Guillaume Louvel, Priyanka Anujan, Vydianathan Ravi, Carine Bonnard, Sebastien Moutton, Patric Schoen, Melanie Fradin, Estelle Colin, Andre Megarbane, Linda Daou, Ghassan Chehab, Sylvie Di Filippo, Caroline Rooryck, Jean-Francois Deleuze, Anne Boland, Nicolas Arribard, Rukiye Eker, Sumanty Tohari, Alvin Yu-Jin Ng, Marlene Rio, Chun Teck Lim, Birgit Eisenhaber, Frank Eisenhaber, Byrappa Venkatesh, Jeanne Amiel, Hugues Roest Crollius, Christopher T. Gordon, Achim Gossler, Sudipto Roy, Tania Attie-Bitach, Martin Blum, Patrice Bouvagnet, Bruno Reversade
Summary: Phylogenomic and genetic analyses have identified an ancestral module of genes expressed specifically in ciliated left-right organizer tissue, which is essential for left-right axis specification in humans and certain vertebrates. CIROP, a newly discovered gene encoding an extracellular protein, is shown to be specifically expressed in ciliated LROs and plays a crucial role in distinguishing left from right in vertebrates. Mutations in the CIROP gene have been found in human patients with recessive situs anomalies, highlighting the importance of this gene in determining left-right asymmetry.
Review
Genetics & Heredity
Veronica van Heyningen
Summary: The author expresses a strong interest in human genetics and highlights the importance of collaboration, clinical observations, and experimental validation in the field.
ANNUAL REVIEW OF GENOMICS AND HUMAN GENETICS
(2022)
Editorial Material
Genetics & Heredity
Vydianathan Ravi, Prashant Shingate, Byrappa Venkatesh
TRENDS IN GENETICS
(2022)
Meeting Abstract
Ophthalmology
Veronica Van Heyningen
ACTA OPHTHALMOLOGICA
(2022)
Article
Multidisciplinary Sciences
Hildegard Nikki Hall, Hemant Bengani, Robert B. Hufnagel, Giuseppe Damante, Morad Ansari, Joseph A. Marsh, Graeme R. Grimes, Alex von Kriegsheim, David Moore, Lisa McKie, Jamalia Rahmat, Catia Mio, Moira Blyth, Wee Teik Keng, Lily Islam, Meriel McEntargart, Marcel M. Mannens, Veronica Van Heyningen, Joe Rainger, Brian P. Brooks, David R. FitzPatrick
Summary: This study reported a rare genetic eye disease associated with the MAB21L1 gene, which is related to classical aniridia. These mutations are associated with severe abnormalities in the eyes and bones. The study also found that these mutations may disrupt eye development through a gain-of-function mechanism.
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)
