Article
Cell Biology
Hwajung Choi, Liu Yang, Yudong Liu, Ju-Kyung Jeong, Eui-Sic Cho
Summary: In this study, the factors and mechanisms of tooth root resorption were investigated using transgenic mice. It was found that cementum thickness and signaling pathways are associated with tooth root resorption. Dickkopf-related protein 1 was identified as a key molecule in the process of tooth root resorption. The study suggests that proper function and sustained signaling are crucial for tooth root homeostasis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Genetics & Heredity
Yiyun Sun, Dandan Xu, Chundong Zhang, Yitao Wang, Lian Zhang, Deqian Qiao, Youquan Bu, Ying Zhang
Summary: This study demonstrates that GLI1/2 mediates the expression of the PRR11-SKA2 gene pair, which serves as an unfavorable prognostic indicator for LSCC patients. This finding opens up new possibilities for combinatorial diagnostic and therapeutic strategies in LSCC.
Review
Cell Biology
Mengmeng Zhou, Jin Jiang
Summary: The Hedgehog (Hh) family of secreted proteins play important roles in embryonic development and adult tissue homeostasis. Dysregulation of Hh signaling is associated with various human diseases. The activation and repression of the transcription factor Cubitus interruptus (Ci)/Gli in the Hh signaling pathway is regulated by phosphorylation, including phosphorylation by protein kinase A (PKA), glycogen synthase kinase 3 (GSK3), and casein kinase 1 (CK1), as well as by Fused (Fu)/Unc-51 like kinase (Ulk) family kinases such as Fu/Ulk3/Stk36. Phosphorylation of Ci/Gli influences its interaction with the Hh pathway repressor Sufu and affects Hh signal transduction.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
B. Baran, K. Kosieradzka, W. Skarzynska, P. Niewiadomski
Summary: In this study, three novel kinases, MRCK alpha, MRCK beta, and MAP4K5, were identified to interact with Gli proteins and phosphorylate Gli2 on multiple sites. It was demonstrated that MRCK alpha/beta kinases regulate Gli proteins, which in turn affects the transcriptional output of the Hedgehog pathway. The research provides important insights into the phosphorylation-based activation mechanisms of Gli proteins.
CELLULAR SIGNALLING
(2023)
Article
Biochemistry & Molecular Biology
Brooke D. Paradise, Vladimir G. Gainullin, Luciana L. Almada, Ashley N. Sigafoos, Sandhya Sen, Renzo E. Vera, Glancis Luzeena Raja Arul, Murat Toruner, David R. Pease, Alina L. Gonzalez, Fatima M. Mentucci, Daniel H. Grasso, Martin E. Fernandez-Zapico
Summary: This study found that overexpression of SUFU enhances GLI1 transcriptional activity and increases the expression of BCL2, thereby promoting the survival ability of PDAC cells. The study also found that SUFU interacts with GLI1 in the nucleus of PDAC cells and both bind to the promoter of BCL2. Therefore, SUFU has a positive regulatory role in promoting GLI1 activity in PDAC, providing a new mechanism for the regulation of the Hh/GLI signaling pathway in cancer cells.
BIOCHEMICAL JOURNAL
(2023)
Article
Medicine, Research & Experimental
Chao Tang, Ximei Wu, Qianlei Ren, Minli Yao, Shouying Xu, Ziyi Yan
Summary: This study uncovers a novel mechanism controlling Hh signaling through the activation of Rac1 and subsequent translocation of Gli. Rac1 activation affects KIF3A phosphorylation and IFT88 stability, thereby disrupting SuFu-Gli complex formation and releasing Gli for nuclear translocation.
Article
Cell Biology
Zhengwei Yan, Minzhang Cheng, Guohui Hu, Yao Wang, Shaopeng Zeng, Aidi Huang, Linlin Xu, Yuan Liu, Chao Shi, Libin Deng, Quqin Lu, Hai Rao, Hua Lu, Ye-Guang Chen, Shiwen Luo
Summary: The study identified SuFu Negating Protein 1 (SNEP1) as a novel target in the Hedgehog (Hh) signaling pathway, promoting colorectal cancer cell proliferation and tumor growth, as well as reducing sensitivity to anti-Hh inhibitors. SNEP1 destabilizes SuFu to regulate Hh signaling, playing a critical role in tumor growth and anti-Hh resistance.
CELL DEATH & DISEASE
(2021)
Review
Immunology
Yazhen Su, Hao Xing, Jie Kang, Linkun Bai, Liyun Zhang
Summary: The Hedgehog (Hh) signaling pathway plays a crucial role in rheumatic diseases and has the potential to be a therapeutic target.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Xiaohua Liu, Yu Zhang, Yalei Li, Juan Wang, Huaqian Ding, Wenjing Huang, Chunyong Ding, Hongchun Liu, Wenfu Tan, Ao Zhang
Summary: Medulloblastoma is a childhood malignant brain tumor with heterogeneous characteristics and lacks effective molecular targeted therapy. Compounds 25 and 35 show high potency in inhibiting hedgehog signaling and interacting with the transcriptional factor GLI, with potential to combat resistant and wild type smoothened receptor.
ACTA PHARMACEUTICA SINICA B
(2021)
Review
Cell Biology
Chamey Suchors, James Kim
Summary: The Hedgehog signaling pathway is crucial for development and tissue regeneration, and its dysregulation has been linked to cancer. Our understanding of this pathway in tumorigenesis has significantly advanced over the past few decades. The pathway can have both tumor-suppressive and oncogenic functions, and noncanonical activation of GLI transcription factors has been observed in certain types of tumors.
Article
Biochemistry & Molecular Biology
Ignazia Tusa, Sinforosa Gagliardi, Alessandro Tubita, Silvia Pandolfi, Alessio Menconi, Matteo Lulli, Persio Dello Sbarba, Barbara Stecca, Elisabetta Rovida
Summary: The study identified a novel Hedgehog-GLI-ERK5 axis that regulates melanoma cell growth, showing that activation of the Hedgehog-GLI pathway increased ERK5 expression and that inhibitors targeting both pathways were more effective in reducing melanoma cell viability and colony formation compared to single treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ting-An Yie, Cynthia A. Loomis, Johannes Nowatzky, Alireza Khodadadi-Jamayran, Ziyan Lin, Michael Cammer, Clea Barnett, Valeria Mezzano, Mark Alu, Jackson A. Novick, John S. Munger, Matthias C. Kugler
Summary: Normal lung development relies on the coordinated action of HH and PDGF signaling pathways, which are crucial for mesenchymal differentiation and proliferation. Recent studies have shown that HH is necessary for alveolar myofibroblast differentiation. In this study, we investigated the relationship between HH and PDGF signaling during postnatal lung development in mice. Our findings suggest that HH and PDGF signaling pathways intersect to support myofibroblast function during secondary alveolar septum formation.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Review
Biology
Fan Yang, Daniel T. Wynn, Chen Shen, Nagi G. Ayad, David J. Robbins
Summary: The Hedgehog signaling pathway is crucial for embryonic development and tissue homeostasis, but its abnormal activation is implicated in various cancers. GLI transcription factors act as the ultimate effectors of this pathway and their activity is regulated by multiple mechanisms. Recent studies have found that novel protein complexes can also regulate the transcriptional activity of GLI proteins, providing innovative druggable targets for GLI-dependent cancers.
Article
Biochemistry & Molecular Biology
Yanran Deng, Dezhen Peng, Jing Xiao, Yunhe Zhao, Wenhao Ding, Shengtao Yuan, Li Sun, Jian Ding, Zizhang Zhou, Meixiao Zhan
Summary: This study reveals a Hh-independent mechanism of SUFU exerting the anti-tumor role by suppressing tumor cell migration through antagonizing ZNF281.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Multidisciplinary Sciences
Rachel K. Lex, Weiqiang Zhou, Zhicheng Ji, Kristin N. Falkenstein, Kaleigh E. Schuler, Kathryn E. Windsor, Joseph D. Kim, Hongkai Ji, Steven A. Vokes
Summary: The study challenges the current understanding that GLI3 repression is established before Hedgehog (HH) signaling and demonstrates that GLI3 is inert in the limb bud before HH onset. The loss of Gli3 does not increase target gene expression or accessibility, contrasting with post-HH signaling. GLI repression is established independently of HH signaling, but after its onset.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
LaGina Nosavanh, Da-Hai Yu, Eric J. Jaehnig, Qiang Tong, Lanlan Shen, Miao-Hsueh Chen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Cell Biology
Olena Zhulyn, Danyi Li, Steven Deimling, Niki Alizadeh Vakili, Rong Mo, Vijitha Puviindran, Miao-Hsueh Chen, Pao-Tien Chuang, Sevan Hopyan, Chi-chung Hui
DEVELOPMENTAL CELL
(2014)
Article
Medicine, Research & Experimental
Da-Hai Yu, Robert A. Waterland, Pumin Zhang, Deborah Schady, Miao-Hsudh Chen, Yongtao Guan, Manasi Gadkari, Lanlan Shen
JOURNAL OF CLINICAL INVESTIGATION
(2014)
Article
Multidisciplinary Sciences
Christopher W. Wilson, Catherine T. Nguyen, Miao-Hsueh Chen, Jehn-Hsiahn Yang, Rhodora Gacayan, Jie Huang, Jau-Nian Chen, Pao-Tien Chuang
Article
Multidisciplinary Sciences
Christopher W. Wilson, Miao-Hsueh Chen, Pao-Tien Chuang
Article
Developmental Biology
MH Chen, L Antoni, R Tazi-Ahnini, M Cork, SJ Ward, CO Båvik
MECHANISMS OF DEVELOPMENT
(2002)
Article
Pediatrics
MH Chen, A MacGowan, S Ward, C Bavik, JJ Greer
BIOLOGY OF THE NEONATE
(2003)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)