Article
Developmental Biology
Christopher J. Derrick, Adrian Santos-Ledo, Lorraine Eley, Isabela Andhika Paramita, Deborah J. Henderson, Bill Chaudhry
Summary: The establishment of left-right axis is crucial for the placement, morphogenesis, and function of internal organs. Cilia-driven fluid flow in the embryonic node is proposed to be dependent on left-right specification. The JNK gene family plays an important role in the development and function of the zebrafish node, determining the length of nodal cilia, generating flow in the node, and restricting southpaw to the left lateral plate mesoderm.
Article
Biology
Pedro Sampaio, Sara Pestana, Catarina Bota, Adan Guerrero, Ivo A. Telley, David Smith, Susana Santos Lopes
Summary: Humans and other vertebrates establish left-right asymmetry during early embryo development. The mechanism behind this process is still not fully understood, but it involves symmetry breaking in the left-right organizer (LRO) through motile cilia-generated fluid flow. Recent experiments in zebrafish embryos revealed a specific time window and flow direction for breaking left-right symmetry. The embryos demonstrate a remarkable ability to recover and circulate new LRO fluid, indicating that fluid dynamics play a crucial role in symmetry breaking.
Article
Cell Biology
Melanie Tingler, Amelie Brugger, Kerstin Feistel, Axel Schweickert
Summary: This study reveals the role of Dmrt2 transcription factor in LRO ciliogenesis and sLRO cell specification in the formation of the vertebrate left-right axis, as well as its functional connection to somitogenesis and LR determination.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Abrar Aljiboury, Eric A. Ingram, Nikhila Krishnan, Favour Ononiwu, Debadrita Pal, Julie S. Manikas, Christopher C. Taveras, Nicole S. Hall, Jonah C. Da Silva, Judy S. Freshour, Heidi C. Hehnly
Summary: Cilia and lumen formation during Danio rerio's left-right organizer (Kupffer's Vesicle, KV) development are acutely coordinated by Rab8, Rab11, and Rab35-mediated membrane trafficking events. Nascent KV cells form cilia at random intracellular positions before extending into the forming lumen, which requires the recruitment of Rab11 by Rab35 positive membranes to modulate CFTR delivery to the apical membrane. Once the lumen reaches a certain size, Arl13b-positive cilia elongate in a Rab8 dependent manner into the lumen.
Review
Cell Biology
Kadeen Forrest, Alexandria C. Barricella, Sonny A. Pohar, Anna Maria Hinman, Jeffrey D. Amack
Summary: Human laterality disorders are caused by embryonic developmental issues. Studying the development of LRO (left-right organizer) in zebrafish models helps us understand the origins of laterality disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Natalia Petri, Rhea Nordbrink, Nikoloz Tsikolia, Stanislav Kremnyov
Summary: Previously, it was believed that there is an early mechanism based on cytoskeletal processes in Xenopus embryos during the early cleavage stages, supported by evidence from chemical inhibition of cytoskeletal protein. However, further analysis revealed that the reported abnormalities only occur after treatment at gastrula-neurula stages, just prior to and during the operation of the left-right organizer. Therefore, these left-right abnormalities cannot be considered as evidence for the early symmetry breaking event in Xenopus embryos.
Article
Multidisciplinary Sciences
Helen M. M. Bellchambers, Amruta R. R. Phatak, Mardi J. J. Nenni, Maria B. B. Padua, Hongyu Gao, Yunlong Liu, Stephanie M. M. Ware
Summary: In this study, the transcriptome of the LRO in mice was defined using single cell RNA sequencing and fluorescent activated cell sorting. The analysis revealed genes associated with cilia and laterality, as well as 127 novel LRO genes. This resource will be valuable for future studies on LRO morphogenesis, laterality establishment, and genetic causes of heterotaxy.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Markus Maerker, Maike Getwan, Megan E. Dowdle, Jason C. McSheene, Vanessa Gonzalez, Jose L. Pelliccia, Danielle S. Hamilton, Valeria Yartseva, Charles Vejnar, Melanie Tingler, Katsura Minegishi, Philipp Vick, Antonio J. Giraldez, Hiroshi Hamada, Rebecca D. Burdine, Michael D. Sheets, Martin Blum, Axel Schweickert
Summary: The authors demonstrate that post-transcriptional regulation of the cilia-driven leftward flow gene dand5 is crucial for symmetry breakage in frogs, fish, and mice, with Pkd2 playing a regulatory role. This process is mediated by a 139 nt Bicc1 responsive element in the dand5 3 ' UTR.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Jing Du, Shu-Kai Li, Liu-Yuan Guan, Zheng Guo, Jiang-Fan Yin, Li Gao, Toru Kawanishi, Atsuko Shimada, Qiu-Ping Zhang, Li-Sha Zheng, Yi-Yao Liu, Xi-Qiao Feng, Lin Zhao, Dong-Yan Chen, Hiroyuki Takeda, Yu-Bo Fan
Summary: This study reveals that heat shock factor 1 (HSF1) is asymmetrically activated in the presence of nodal flow in zebrafish embryos. Deficiency in HSF1 expression leads to situs inversus and disrupted gene expression asymmetry of nodal signaling proteins. Additionally, cilia and Ca(2+) Akt signaling axis are crucial for the activation of HSF1 under mechanical stress.
Article
Developmental Biology
Jason J. Gokey, Agnik Dasgupta, Jeffrey D. Amack
DEVELOPMENTAL BIOLOGY
(2015)
Article
Anatomy & Morphology
Jason J. Gokey, Yongchang Ji, Hwee Goon Tay, Bridget Litts, Jeffrey D. Amack
DEVELOPMENTAL DYNAMICS
(2016)
Article
Multidisciplinary Sciences
Pulin Li, Jamie L. Lahvic, Vera Binder, Emily K. Pugach, Elizabeth B. Riley, Owen J. Tamplin, Dipak Panigrahy, Teresa V. Bowman, Francesca G. Barrett, Garrett C. Heffner, Shannon McKinney-Freeman, Thorsten M. Schlaeger, George Q. Daley, Darryl C. Zeldin, Leonard I. Zon
Article
Developmental Biology
Yongchang Ji, Sharleen M. Buel, Jeffrey D. Amack
DEVELOPMENTAL BIOLOGY
(2016)
Review
Biology
Agnik Dasgupta, Jeffrey D. Amack
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2016)
Article
Multidisciplinary Sciences
Andrew E. Jacob, Christopher E. Turner, Jeffrey D. Amack
Article
Developmental Biology
Carolyn T. Chang, Jeffrey D. Amack, Christopher M. Whipps
Article
Cell & Tissue Engineering
Xin Gao, Tongyu Wu, Kirby D. Johnson, Jamie L. Lahvic, Erik A. Ranheim, Leonard I. Zon, Emery H. Bresnick
Article
Biology
Agnik Dasgupta, Matthias Merkel, Madeline J. Clark, Andrew E. Jacob, Jonathan Edward Dawson, M. Lisa Manning, Jeffrey D. Amack
Article
Developmental Biology
Sarah C. Rothschild, Jamie Lahvic, Ludmila Francescatto, Jamie J. A. McLeod, Shawn M. Burgess, Robert M. Tombes
DEVELOPMENTAL BIOLOGY
(2013)
Article
Cell Biology
Daqing Jin, Terri T. Ni, Jianjian Sun, Haiyan Wan, Jeffrey D. Amack, Guangju Yu, Jonathan Fleming, Chin Chiang, Wenyan Li, Anna Papierniak, Satish Cheepala, Gwenaelle Conseil, Susan P. C. Cole, Bin Zhou, Iain A. Drummond, John D. Schuetz, Jarema Malicki, Tao P. Zhong
NATURE CELL BIOLOGY
(2014)
Article
Multidisciplinary Sciences
Jamie L. Lahvic, Michelle Ammerman, Pulin Li, Megan C. Blair, Emma R. Stillman, Eva M. Fast, Anne L. Robertson, Constantina Christodoulou, Julie R. Perlin, Song Yang, Nan Chiang, Paul C. Norris, Madeleine L. Daily, Shelby E. Redfield, Iris T. Chang, Mona Chatrizeh, Michael E. Chase, Olivia Weis, Yi Zhou, Charles N. Serhan, Leonard I. Zon
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Cell Biology
Jamie L. Lahvic, Iswar K. Hariharan
MOLECULAR BIOLOGY OF THE CELL
(2019)
Article
Developmental Biology
Andrew E. Jacob, Jeffrey D. Amack, Christopher E. Turner
DEVELOPMENTAL BIOLOGY
(2017)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)