期刊
DEVELOPMENTAL BIOLOGY
卷 375, 期 1, 页码 65-78出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2012.12.014
关键词
Drosophila melanogaster; Gcm/Glide; FLAG-tagging; AGO1; MicroRNAs; Peritracheal cells; BAC recombineering
资金
- Institut National de la Sante et de la Recherche Medicate
- Centre National de la Recherche Scientifique
- Universite de Strasbourg
- Hopital de Strasbourg
- Association pour la Recherche sur le Cancer
- Institut National du Cancer
- Agence Nationale de la Recherche
- Region Alsace
- Fondation pour la Recherche Medicate en France
- Association contre le Cancer
- Centre Europeen de Recherche en Biologie et en Medecine
- Agence National contre le Cancer
In Drosophila, the transcription factor Gcm/Glide plays a key role in cell fate determination and cellular differentiation. In light of its crucial biological impact, major efforts have been put for analyzing its properties as master regulator, from both structural and functional points of view. However, the lack of efficient antibodies specific to the Gcm/Glide protein precluded thorough analyses of its regulation and activity in vivo. In order to relieve such restraints, we designed an epitope-tagging approach to FLAG-recognize and analyze the functional protein both in vitro (exogenous Gcm/Glide) and in vivo (endogenous protein). We here (i) reveal a tight interconnection between the small RNA and the Gcm/Glide pathways. AGO1 and miR-1 are Gcm/Glide targets whereas miR-279 negatively controls Gcm/Glide expression (ii) identify a novel cell population, peritracheal cells, expressing and requiring Gcm/Glide. Peritracheal cells are non-neuronal neurosecretory cells that are essential in ecdysis. In addition to emphasizing the importance of following the distribution and the activity of endogenous proteins in vivo, this study provides new insights and a novel frame to understand the Gcm/Glide biology. (C) 2012 Elsevier Inc. All rights reserved.
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