Article
Developmental Biology
Benjamin C. Stark, Yuanyuan Gao, Diane S. Sepich, Lakyn Belk, Matthew A. Culver, Bo Hu, Marlene Mekel, Wyndham Ferris, Jimann Shin, Lilianna Solnica-Krezel, Fang Lin, John A. Cooper
Summary: Analysis of zebrafish carrying mutations of carmil3 revealed impaired endodermal and DFC migration during gastrulation, affecting KV morphogenesis. Mutant KVs displayed smaller size, reduced cell numbers, and decreased cilia, leading to defects in L/R patterning with varying penetrance and expressivity. These findings suggest a new role for CARMIL3 in vertebrate embryo morphogenesis, involving endodermal cell and DFC migration, as well as KV and L/R asymmetry formation.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Christopher J. Derrick, Adrian Santos-Ledo, Lorraine Eley, Isabela Andhika Paramita, Deborah J. Henderson, Bill Chaudhry
Summary: The establishment of left-right axis is crucial for the placement, morphogenesis, and function of internal organs. Cilia-driven fluid flow in the embryonic node is proposed to be dependent on left-right specification. The JNK gene family plays an important role in the development and function of the zebrafish node, determining the length of nodal cilia, generating flow in the node, and restricting southpaw to the left lateral plate mesoderm.
Article
Biochemistry & Molecular Biology
Ines Oliveira, Raquel Jacinto, Sara Pestana, Fernando Nolasco, Joaquim Calado, Susana Santos Lopes, Monica Roxo-Rosa
Summary: This study demonstrates that knocking down PC2 affects CFTR-mediated inflation, with KV enlargement rescued by injection of Xenopus pkd2 mRNA and tolvaptan treatment. The findings support the use of KV as an in vivo model for screening compounds that may prevent cyst enlargement in ciliopathies through CFTR inhibition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Feng Lv, Xiaojuan Ge, Peipei Qian, Xiaofeng Lu, Dong Liu, Changsheng Chen
Summary: The study reveals that Nav3 plays a critical role in the cardiac development of zebrafish embryos. Deletion of the nav3 gene leads to severe deficiencies in the morphology and structure of the heart in zebrafish.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Developmental Biology
Alessio Paolini, Dinara Sharipova, Tim Lange, Salim Abdelilah-Seyfried
Summary: This study reveals that the paralogous genes wnt9a/b and both canonical and non-canonical Wnt signaling pathways are required for the migration of cardiomyocyte progenitor cells and the formation of the cardiac tube in zebrafish. They regulate the timing of cardiac progenitor cell differentiation and activate downstream pathways to direct cardiac morphogenesis.
Review
Cell Biology
Anushka Deshpande, Prithviraj Manohar Vijaya Shetty, Norbert Frey, Ashraf Yusuf Rangrez
Summary: This article discusses the molecular mechanisms that regulate cardiac development and adult cardiac homeostasis, focusing on the role of SRF and its potential therapeutic applications.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Cell Biology
Michka Sharpe, Juan Manuel Gonzalez-Rosa, Felicia Wranitz, Spencer Jeffrey, Katherine Copenhaver, C. Geoffrey Burns, Caroline E. Burns
Summary: Cardiomyocyte proliferation is an important source of new myocardium during heart development and regeneration, and Ruvbl2 functions as a suppressor of cardiomyocyte proliferation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Pedro Sampaio, Sara Pestana, Catarina Bota, Adan Guerrero, Ivo A. Telley, David Smith, Susana Santos Lopes
Summary: Humans and other vertebrates establish left-right asymmetry during early embryo development. The mechanism behind this process is still not fully understood, but it involves symmetry breaking in the left-right organizer (LRO) through motile cilia-generated fluid flow. Recent experiments in zebrafish embryos revealed a specific time window and flow direction for breaking left-right symmetry. The embryos demonstrate a remarkable ability to recover and circulate new LRO fluid, indicating that fluid dynamics play a crucial role in symmetry breaking.
Article
Biochemistry & Molecular Biology
Ekambaram Perumal, Sangavi Eswaran, Reshma Parvin, Satheeswaran Balasubramanian
Summary: The study showed that ferulic acid could mitigate arsenic-induced cardiotoxicity in zebrafish larvae, reducing arsenic content and congenital deformities, such as cardiac malformations. Ferulic acid supplementation reversed abnormal cardiac function and reduced acetylcholinesterase activity induced by arsenic exposure, indicating its protective effects on cardiac malformations.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Cell Biology
Takafumi Ikeda, Kiichi Inamori, Toru Kawanishi, Hiroyuki Takeda
Summary: This study established a new zebrafish transgenic line and revealed the differentiation potency of KV-epithelial cells and their descendants after the collapse of KV. It was found that they undergo epithelial-mesenchymal transition and contribute to mesodermal lineages through transdifferentiation in the later stages of development.
DEVELOPMENT GROWTH & DIFFERENTIATION
(2022)
Review
Biochemistry & Molecular Biology
Boni A. Afouda
Summary: Transcription factors, particularly GATA4, 5, and 6, play crucial roles in heart induction, formation, growth, and morphogenesis. These genes are expressed in developing hearts and have overlapping functions. Recent research has identified specific genes regulated by each family member and elucidated their distinct roles in heart development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Stamatiki Katraki-Pavlou, Pinelopi Kastana, Dimitris Bousis, Despoina Ntenekou, Aimilia Varela, Constantinos H. Davos, Sophia Nikou, Eleni Papadaki, Grigorios Tsigkas, Emmanouil Athanasiadis, Gonzalo Herradon, Constantinos M. Mikelis, Dimitris Beis, Evangelia Papadimitriou
Summary: Protein tyrosine phosphatase receptor-zeta 1 (PTPRZ1) is expressed in fetal but not adult heart and seems to affect heart development. In both mouse and zebrafish animal models, loss of PTPRZ1 results in dilated left ventricle cavity, decreased ejection fraction, and fraction shortening, with no signs of cardiac hypertrophy. PTPRZ1 also seems to be involved in atrioventricular canal specification, outflow tract morphogenesis, and heart angiogenesis. These results suggest that PTPRZ1 plays a role in heart development and support the hypothesis that it may be involved in congenital cardiac pathologies.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Christopher J. Derrick, Juliana Sanchez-Posada, Farah Hussein, Federico Tessadori, Eric J. G. Pollitt, Aaron M. Savage, Robert N. Wilkinson, Timothy J. Chico, Fredericus J. van Eeden, Jeroen Bakkers, Emily S. Noel
Summary: This study reveals the existence of regionalized extracellular matrix (ECM) expansion in the heart tube, which is crucial for proper heart development. The study also uncovers the role of laterality cues in ECM asymmetry in the heart. These findings provide a new model that suggests the interaction between embryonic laterality and ECM expansion pathways to promote cardiac morphogenesis.
CARDIOVASCULAR RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Anna Contato, Onelia Gagliano, Michael Magnussen, Monica Giomo, Nicola Elvassore
Summary: In this study, a new method for cardiac differentiation-on-a-chip was developed by combining stage-specific regulation of Wnt/beta-catenin signaling with forced expression of transcription factors. The results showed that this optimized protocol led to a robust and reproducible approach to obtain a cost-effective hiPSC-derived heart-on-chip model, enabling high-throughput experiments on functional cardiomyocytes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Developmental Biology
Ayele Taddese Tsedeke, Srinivas Allanki, Alessandra Gentile, Vanesa Jimenez-Amilburu, Seyed Javad Rasouli, Stefan Guenther, Shih-Lei Lai, Didier Y. R. Stainier, Ruben Marin-Juez
Summary: Zebrafish have the ability to regenerate their hearts, with CMs capable of dedifferentiating and proliferating to replenish lost muscle. Comparative transcriptomic analyses identified tnnc2 and tnni4b.3 as markers for CMs at different developmental stages, shedding light on the dynamics of CM populations.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Rheumatology
Subhrajit Saha, Juana Gonzalez, Gabriel Rosenfeld, Harold Keiser, Elena Peeva
ARTHRITIS AND RHEUMATISM
(2009)
Article
Oncology
Sean F. Altekruse, Gabriel E. Rosenfeld, Danielle M. Carrick, Emilee J. Pressman, Sheri D. Schully, Leah E. Mechanic, Kathleen A. Cronin, Brenda Y. Hernandez, Charles F. Lynch, Wendy Cozen, Muin J. Khoury, Lynne T. Penberthy
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2014)
Article
Biochemistry & Molecular Biology
Vikas Gupta, Matthew Gemberling, Ravi Karra, Gabriel E. Rosenfeld, Todd Evans, Kenneth D. Poss
Article
Developmental Biology
Audrey Holtzinger, Gabriel E. Rosenfeld, Todd Evans
DEVELOPMENTAL BIOLOGY
(2010)
Article
Genetics & Heredity
Huann-Sheng Chen, Carolyn M. Hutter, Leah E. Mechanic, Christopher I. Amos, Vineet Bafna, Elizabeth R. Hauser, Ryan D. Hernandez, Chun Li, David A. Liberles, Kimberly McAllister, Jason H. Moore, Dina N. Paltoo, George J. Papanicolaou, Bo Peng, Marylyn D. Ritchie, Gabriel Rosenfeld, John S. Witte, Elizabeth M. Gillanders, Eric J. Feuer
GENETIC EPIDEMIOLOGY
(2015)
Article
Rheumatology
Yu Zhang, Subhrajit Saha, Gabriel Rosenfeld, Juana Gonzalez, Kiril P. Pepeljugoski, Elena Peeva
JOURNAL OF RHEUMATOLOGY
(2010)
Article
Multidisciplinary Sciences
Gabriel Rosenfeld, Andrei Gabrielian, Qinlu Wang, Jingwen Gu, Darrell E. Hurt, Alyssa Long, Alex Rosenthal
Summary: The study analyzed TB cases using clinical characteristics, CT images, and radiologist observations from a database, finding that these observations can predict treatment outcomes. It also identified markers of TB disease spread associated with poor treatment outcomes, suggesting the need for further data collection to address potential confounding factors.
Meeting Abstract
Medicine, General & Internal
E. Peeva, G. E. Rosenfeld, J. Gonzalez, S. Saha
JOURNAL OF INVESTIGATIVE MEDICINE
(2007)
Meeting Abstract
Rheumatology
Gabriel Rosenfeld, Juana Gonzalez, Subhrajit Saba, Yu Zhang, Elena Peeva
ARTHRITIS AND RHEUMATISM
(2006)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)