Article
Multidisciplinary Sciences
Simon Chewchuk, Sanzida Jahan, David Lohnes
Summary: The intestinal epithelium plays a crucial role in maintaining gut homeostasis, combating infections, and promoting regeneration. Cdx2 and Cdx1 are key factors in intestinal homeostasis, with Cdx2 loss leading to dysregulation of iCD8 alpha cells dependent on H2-T3 and subsequent inflammatory responses.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Ian Y. Luk, Laura J. Jenkins, Kael L. Schoffer, Irvin Ng, Janson W. T. Tse, Dmitri Mouradov, Stanislaw Kaczmarczyk, Rebecca Nightingale, Allan D. Burrows, Robin L. Anderson, Diego Arango, Higinio Dopeso, Larry Croft, Mark F. Richardson, Oliver M. Sieber, Yang Liao, Jennifer K. Mooi, Natalia Vukelic, Camilla M. Reehorst, Shoukat Afshar-Sterle, Vicki L. J. Whitehall, Lochlan Fennell, Helen E. Abud, Niall C. Tebbutt, Wayne A. Phillips, David S. Williams, Wei Shi, Lisa A. Mielke, Matthias Ernst, Amardeep S. Dhillon, Nicholas J. Clemons, John M. Mariadason
Summary: In this study, co-ordinate epigenetic inactivation of two epithelial-specific transcription factors, EHF and CDX1, was identified as a mechanism driving differentiation loss in CRCs. Re-expression of EHF and CDX1 induced extensive chromatin remodelling, transcriptional re-programming, and differentiation in poorly-differentiated CRC cells, leading to reduced growth and metastasis. These findings reveal a novel mechanism driving epithelial de-differentiation and tumor progression in CRC.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Genetics & Heredity
Yalun Zhu, Alexa Hryniuk, Tanya Foley, Bradley Hess, David Lohnes
Summary: Loss-of-function mutations in APC contribute to malignant progression of colorectal polyps, while the absence of Cdx2 is associated with more aggressive subtypes of colorectal cancer. Simultaneous loss of Cdx1 and Cdx2 in mice leads to an increase in intestinal polyps. In SW480 colorectal cancer cells, loss of Cdx2 affects the Notch signaling pathway and results in EphrinB1 loss, potentially influencing colorectal cancer through a Notch-dependent mechanism.
Article
Multidisciplinary Sciences
Jaroslav Ferenc, Panagiotis Papasaikas, Jacqueline Ferralli, Yukio Nakamura, Sebastien Smallwood, Charisios D. Tsiairis
Summary: The study demonstrates the importance of tissue stretching during inflation for the appearance of the head organizer Wnt3 in Hydra tissue spheroids, with Wnt3 overexpression enabling successful regeneration in the absence of mechanical stimulation. This highlights the significance of mechanochemical feedback loops for patterning epithelial lumens.
Article
Cell Biology
Chika Ogura, Shoko Nishihara
Summary: This study demonstrated the important role of DS in maintaining the undifferentiated state of mESCs and influencing their differentiation direction and Wnt signaling pathway. Knockdown or overexpression experiments targeting the D4ST1 gene revealed the mechanism of DS in mESCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Katharine Goodwin, Jacob M. Jaslove, Hirotaka Tao, Min Zhu, Sevan Hopyan, Celeste M. Nelson
Summary: By using single-cell RNA-sequencing of embryonic mouse lungs, this study reveals that the pulmonary mesenchyme contains a continuum of cell identities, but no transcriptionally distinct progenitors. Transcriptional variability is correlated with spatially distinct sub-epithelial and sub-mesothelial mesenchymal compartments that are regulated by Wnt signaling. Live-imaging and tension-sensors show that sub-epithelial mesenchyme contributes to airway smooth muscle. Reconstruction of differentiation trajectories reveals early activation of cytoskeletal and Wnt signaling genes.
Review
Oncology
Jordi Badia-Ramentol, Francisco Gimeno-Valiente, Elena Durendez, Carolina Martinez-Ciarpaglini, Jenniffer Linares, Mar Iglesias, Andres Cervantes, Alexandre Calon, Noelia Tarazona
Summary: Adjuvant chemotherapy is the main treatment option for localized colorectal cancer (CRC), but many patients have unfavorable outcomes. CDX2, a biomarker of prognosis and relapse, is associated with the complexity of CRC. Immunohistochemistry evaluation of CDX2 can identify high-risk CRC patients, but assessing its expression remains challenging in many patients.
CANCER TREATMENT REVIEWS
(2023)
Article
Multidisciplinary Sciences
Mikihito Shibata, Kartik Pattabiraman, Belen Lorente-Galdos, David Andrijevic, Suel-Kee Kim, Navjot Kaur, Sydney K. Muchnik, Xiaojun Xing, Gabriel Santpere, Andre M. M. Sousa, Nenad Sestan
Summary: The study highlights the crucial role of retinoic acid signaling in the development of the prefrontal cortex (PFC) and suggests its potential impact on the evolutionary expansion of the PFC. This research provides insights into the molecular mechanisms underlying cognitive flexibility and working memory, as well as disorders such as autism and schizophrenia.
Article
Multidisciplinary Sciences
Takahiro Koide, Michiyo Koyanagi-Aoi, Keiichiro Uehara, Yoshihiro Kakeji, Takashi Aoi
Summary: This study demonstrated that the overexpression of CDX2 in human gastric organoids induced intestinal metaplasia, providing insights into the mechanisms of intestinal metaplasia and carcinogenesis in human gastric mucosa.
Article
Multidisciplinary Sciences
Thomas P. Lozito, Ricardo Londono, Aaron X. Sun, Megan L. Hudnall
Summary: Regenerated lizard tails lack the dorsoventral patterning achieved during embryonic development, even though they are able to form cartilage during regeneration.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Maayan Pour, Abhishek Sampath Kumar, Naama Farag, Adriano Bolondi, Helene Kretzmer, Maria Walther, Lars Wittler, Alexander Meissner, Iftach Nachman
Summary: This study investigates the onset of definitive endoderm (DE) using a dual Bra/Sox17 reporter cell line. It reveals that Sox17 expression initiates in isolated cells within a specific temporal window in vivo, while in vitro models show that DE cells emerge from mesendoderm progenitors in a regular but spatially stochastic pattern, which is arranged by self-sorting of Sox17+ cells. The self-sorting process coincides with upregulation of E-cadherin but is not essential for DE differentiation or proliferation.
Article
Gastroenterology & Hepatology
Tianyun Long, Nazia Abbasi, Juan E. Hernandez, Yuxin Li, Ibrahim M. Sayed, Shengyun Ma, Attilio Iemolo, Brian A. Yee, Gene W. Yeo, Francesca Telese, Pradipta Ghosh, Soumita Das, Wendy Jia Men Huang
Summary: The RNA binding protein DDX5 directs tuft cell specification and function to regulate microbial repertoire and disease susceptibility in the intestine.
Article
Genetics & Heredity
Laura Rojas-Lopez, Sascha Krakovka, Elin Einarsson, Ulf Ribacke, Feifei Xu, Jon Jerlstrom-Hultqvist, Staffan G. Svard
Summary: Giardia intestinalis, an intestinal protozoan parasite causing diarrheal infections worldwide, undergoes significant gene expression changes during late encystation, with major up-regulation of cyst wall proteins and sequential up-regulation of transcription factors. This high-resolution gene expression map of Giardia encystation provides valuable insights for further studies of this important differentiation process.
Article
Biochemistry & Molecular Biology
Laura Marruecos, Joan Bertran, Daniel Alvarez-Villanueva, Maria Carmen Mulero, Yolanda Guillen, Luis G. Palma, Martin Floor, Anna Vert, Sara Arce-Gallego, Irene Pecharroman, Laura Batlle, Jordi Villa-Freixa, Gourisankar Ghosh, Anna Bigas, Lluis Espinosa
Summary: I kappa Bs act as cytoplasmic inhibitors of NF-kB transcription factors. Phosphorylated and SUMOylated I kappa B alpha binds to histones H2A and H4, influencing chromatin association and transcriptional regulation in skin and intestine stem cells and progenitor cells. Dynamic binding of I kappa B alpha to chromatin is necessary for intestinal cell differentiation, providing insight into the restricted nuclear distribution of p-I kappa B alpha in specific stem cell compartments.
Article
Biochemistry & Molecular Biology
Rulan Jiang, Jamie Lo, Christine Prell, Bo Loennerdal
Summary: This study found that oral intake of milk containing OPN can promote intestinal development in early life by increasing cell proliferation and activating cellular signaling pathways.
Article
Cell Biology
Stephanie Grainger, Jenna Richter, Raquel Espin Palazon, Claire Pouget, Brianna Lonquich, Sara Wirth, Kathrin Sabine Grassme, Wiebke Herzog, Matthew R. Swift, Brant M. Weinstein, David Traver, Karl Willert
Article
Genetics & Heredity
Rea M. Lardelli, Ashleigh E. Schaffer, Veerle R. C. Eggens, Maha S. Zaki, Stephanie Grainger, Shashank Sathe, Eric L. Van Nostrand, Zinayida Schlachetzki, Basak Rosti, Naiara Akizu, Eric Scott, Jennifer L. Silhavy, Laura Dean Heckman, Rasim Ozgur Rosti, Esra Dikoglu, Anne Gregor, Alicia Guemez-Gamboa, Damir Musaev, Rohit Mande, Ari Widjaja, Tim L. Shaw, Sebastian Markmiller, Isaac Marin-Valencia, Justin H. Davies, Linda de Meirleir, Hulya Kayserili, Umut Altunoglu, Mary Louise Freckmann, Linda Warwick, David Chitayat, Susan Blaser, Ahmet Okay Caglayan, Kaya Bilguvar, Huseyin Per, Christina Fagerberg, Henrik T. Christesen, Maria Kibaek, Kimberly A. Aldinger, David Manchester, Naomichi Matsumoto, Kazuhiro Muramatsu, Hirotomo Saitsu, Masaaki Shiina, Kazuhiro Ogata, Nicola Foulds, William B. Dobyns, Neil C. Chi, David Traver, Luigina Spaccini, Stefania Maria Bova, Stacey B. Gabrie, Murat Gunel, Enza Maria Valente, Marie-Cecile Nassogne, Eric J. Bennett, Gene W. Yeo, Frank Baas, Jens Lykke-Andersen, Joseph G. Gleeson
Article
Developmental Biology
Stephanie Grainger, Brianna Lonquich, Chet Huan Oon, Nicole Nguyen, Karl Willert, David Traver
Review
Medicine, Research & Experimental
Stephanie Grainger, Karl Willert
WILEY INTERDISCIPLINARY REVIEWS-SYSTEMS BIOLOGY AND MEDICINE
(2018)
Article
Biochemistry & Molecular Biology
Alexa Hryniuk, Stephanie Grainger, Joanne G. A. Savory, David Lohnes
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Multidisciplinary Sciences
Stephanie Grainger, Alexa Hryniuk, David Lohnes
Article
Multidisciplinary Sciences
Cara Bickers, Sophia D. Espanola, Stephanie Grainger, Claire Pouget, David Traver
Editorial Material
Cell Biology
Stephanie Grainger, David Traver
DEVELOPMENTAL CELL
(2019)
Article
Cell Biology
Stephanie Grainger, Nicole Nguyen, Jenna Richter, Jordan Setayesh, Brianna Lonquich, Chet Huan Oon, Jacob M. Wozniak, Rocio Barahona, Caramai N. Kamei, Jack Houston, Marvic Carrillo-Terrazas, Iain A. Drummond, David Gonzalez, Karl Willert, David Traver
NATURE CELL BIOLOGY
(2019)
Meeting Abstract
Biochemistry & Molecular Biology
Peyton McElhone, Alexa Hryniuk, Carol Nichols, Anna Edmondson
Meeting Abstract
Biochemistry & Molecular Biology
Madison Stokes, Alexa Hryniuk, Anna Edmondson
Meeting Abstract
Biochemistry & Molecular Biology
Emma Burke, Alexa Hryniuk, Anna Edmondson
Meeting Abstract
Biochemistry & Molecular Biology
Catherine Will, Anna Edmondson, Alexa Hryniuk
Article
Genetics & Heredity
Yalun Zhu, Alexa Hryniuk, Tanya Foley, Bradley Hess, David Lohnes
Summary: Loss-of-function mutations in APC contribute to malignant progression of colorectal polyps, while the absence of Cdx2 is associated with more aggressive subtypes of colorectal cancer. Simultaneous loss of Cdx1 and Cdx2 in mice leads to an increase in intestinal polyps. In SW480 colorectal cancer cells, loss of Cdx2 affects the Notch signaling pathway and results in EphrinB1 loss, potentially influencing colorectal cancer through a Notch-dependent mechanism.
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)