Article
Cell Biology
Anna Ashton, Jason Clark, Julia Fedo, Angelo Sementilli, Yara D. D. Fragoso, Peter McCaffery
Summary: The pineal gland regulates the circadian timing system through melatonin production. The study investigates the interaction between the pineal gland and retinoic acid (RA) signaling system. It is found that there is conservation of the RA signaling system in mammalian species, but RA does not have a short-term effect on clock gene expression. Unexpectedly, melatonin was found to suppress the induction of gene transcription by RA.
Article
Nutrition & Dietetics
Wenjia Zhang, Qingyun Peng, Xiaoyu Zhang, Jiaxu Guo, Huili Tong, Shuang Li
Summary: Vitamin A is important for cell growth and development, but its role in muscle injury repair and molecular mechanism are unclear. This study found that Vitamin A promotes differentiation of C2C12 cells and increases RARa expression. In a mouse model of skeletal muscle injury, Vitamin A significantly accelerated repair and increased RARa expression. The study provides a theoretical basis for understanding Vitamin A-mediated muscle development and developing therapeutic drugs for muscle diseases in animals.
Article
Biochemistry & Molecular Biology
Shachi Pranjal Vyas, Ritobrata Goswami
Summary: Vitamins play a crucial role in regulating the differentiation of T helper cells, with calcitriol and retinoic acid exhibiting hormone-like properties and antagonizing each other in this process. The recruitment of PU.1 to the Il9 gene is significantly impaired in the presence of either vitamin alone, but their combined treatment increases the recruitment. Interestingly, retinoic acid dampens the binding of VDR on the Il9 gene, negating the effect of calcitriol.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Xinyue Zhang, Yan Cong, Zhaowei Chu, Linjing Shi, Yi Zheng, Qiang Zhao, Songmei Geng, Kun Guo
Summary: The study found that aberrant TET2 led to hypermethylation of the RAR beta promoter, which contributed to the deficiency of RAR beta in CSCC. Increasing the expression of TET2 could reverse promoter hypermethylation, increase the expression of RAR beta, and enhance tumor cells' sensitivity to retinoic acid treatment.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Reza Zolfaghari, Jessica A. Bonzo, Frank J. Gonzalez, A. Catharine Ross
Summary: HNF4 alpha regulates lipid and glucose metabolism genes. RA can act as an antagonist for HNF4 alpha in genes lacking RARE and as an agonist for RARE-containing genes. Overall, RA may interfere with HNF4 alpha function and deregulate genes important for lipid and glucose metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Trey Polvadore, Malcolm Maden
Summary: This study reveals the impact of retinoic acid treatment on the control of positional information during axolotl limb regeneration. Through RNA sequencing, the genes interacting with RAR alpha were identified, shedding light on the mechanism of action for future research.
Article
Oncology
Elhusseiny Mohamed Mahmoud Abdelwahab, Judit Bovari-Biri, Gabor Smuk, Tunde Harko, Janos Fillinger, Judit Moldvay, Vera P. Krymskaya, Judit E. Pongracz
Summary: The study found that TSC mutation leads to dysregulation of vitamin A metabolic enzymes and downregulation of RARβ, while RA can restore RARβ levels and limit cell migration with minimal effect on proliferation. Combination of RA and rapamycin can normalize enzyme expression and activity, restore RARβ expression, and reduce cell proliferation and migration.
FRONTIERS IN ONCOLOGY
(2021)
Article
Toxicology
I. Alvarado-Morales, V. Olivares-Illana, C. Arenas-Huertero, E. Reynaga-Hernandez, E. Layseca-Espinosa, E. J. Tokar, C. Escudero-Lourdes
Summary: The study suggests that arsenic may impair the TLR3-associated anti-tumor pathway activated by 9cRA and PIC in prostate cancer stem cells and epithelial cells, leading to unresponsiveness to therapy.
TOXICOLOGY LETTERS
(2021)
Review
Biochemistry & Molecular Biology
Mariam Rusidze, Marine Adlanmerini, Elodie Chantalat, I. Raymond-Letron, Surya Cayre, Jean-Francois Arnal, Marie-Ange Deugnier, Francoise Lenfant
Summary: This review discusses the role of ER alpha in mammary gland development and breast cancer progression, highlighting both the classical nuclear genomic and membrane non-genomic actions of ER alpha in regulating mammary development at different stages. It also provides an overview of the characteristics of luminal cell lineage expressing ER alpha and the impact of ER alpha signaling in transgenic mouse models, emphasizing its pivotal role in mammary gland morphogenesis and function as well as its implication in tumorigenesis. Additionally, the review covers the main features of ER alpha-positive luminal breast cancers and their modeling in mice.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Cell Biology
Elena Spina, Pamela Cowin
Summary: Embryonic mammary gland development involves multiple steps, including the formation of mammary placodes and mammary buds, as well as the development of ductal trees. Studies have identified factors and pathways that influence these steps, and there is a deeper understanding of the characteristics of mammary stem cells and progenitor cells.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Swarajit Kumar Biswas, Saswati Banerjee, Ginger Wendolyn Baker, Chieh-Yin Kuo, Indrajit Chowdhury
Summary: The mammary gland, a compound and branched structure, plays a crucial role in mammalian reproduction by producing milk. Its development involves hormonal regulation and different stages of morphogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Xintong Dai, Jing Jin, Yan Jia, Kai Yang, Jingxia Han, Zhiyuan Zhang, Xiujuan Ding, Cheng Yao, Tao Sun, Caibin Zhu, Huijuan Liu
Summary: This study identified a novel RAR-gamma-selective agonist, tectorigenin, which can effectively inhibit UV-induced skin damage and has positive effects on inflammation and photoaging. It is safer and has fewer side effects compared to ATRA. Furthermore, nano-particle loaded tectorigenin improves the utilization of tectorigenin.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Diana Wu, Lilian U. Thompson, Elena M. Comelli
Summary: Breast cancer, the second most common cancer in women after skin cancer, can be influenced by mammary gland development. miRNAs play a key role in both mammary development and breast cancer, and studying their role in development may provide insights into potential targets for breast cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Uijin Kim, Suyeon Kim, Nahyun Kim, Ha Youn Shin
Summary: The activation of mammary-specific super-enhancers relies on protein-protein interactions between mammary-enriched transcription factors, and ELF5 and STAT5A play key roles in the activation of the super-enhancers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Allergy
Josephine Scholz, Julia Kuhrau, Frederik Heinrich, Gitta Anne Heinz, Andreas Hutloff, Margitta Worm, Guido Heine
Summary: Vitamin A signaling via RAR alpha impacts allergen-specific immunoglobulin response by directly affecting B cell differentiation and indirectly by promoting T follicular helper cells.
Article
Cell Biology
Tingsheng Yu, Oscar Cazares, Alison D. Tang, Hyun-Yi Kim, Tomas Wald, Adya Verma, Qi Liu, Mary Helen Barcellos-Hoff, Stephen N. Floor, Han-Sung Jung, Angela N. Brooks, Ophir D. Klein
Summary: The study reveals that SRSF1 protein plays a crucial role in supporting adult tissue homeostasis and renewal by controlling specific alternative splicing events, particularly in dental proliferation and progenitor cell survival. This regulatory mechanism is also found to be conserved in other adult epithelial tissues, demonstrating the importance of SRSF1 in different organs.
DEVELOPMENTAL CELL
(2022)
Article
Biochemistry & Molecular Biology
Kamini Kaushal, Apoorvi Tyagi, Janardhan Keshav Karapurkar, Eun-Jung Kim, Parthasaradhireddy Tanguturi, Kye-Seong Kim, Han-Sung Jung, Suresh Ramakrishna
Summary: The study demonstrates that ubiquitin-specific protease 11 (USP11) is a key regulator of MSX1 protein in osteogenic differentiation. USP11 interacts with MSX1 and prevents its degradation through deubiquitinating activity, enhancing the differentiation potential of human mesenchymal stem cells. These findings suggest USP11 as a potential target for bone regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kamini Kaushal, Eun-Jung Kim, Apoorvi Tyagi, Janardhan Keshav Karapurkar, Saba Haq, Han-Sung Jung, Kye-Seong Kim, Suresh Ramakrishna
Summary: The deubiquitinating enzyme USP49 interacts with and stabilizes the protein levels of PAX9 and MSX1, promoting successful tooth development.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Biochemistry & Molecular Biology
Anish Ashok Adpaikar, Sushan Zhang, Hyun-Yi Kim, Ki Woo Kim, Seok Jun Moon, Jong-Min Lee, Han-Sung Jung
Summary: In this study, a suspension-culture method was established to fine-tune taste bud organoids by altering their apicobasal polarity, enabling the accessible localization of taste receptor cells. These suspension-cultured organoids showed comparable differentiation and renewal rates to those of taste buds in vivo, and exhibited functional taste receptor cells and cycling progenitor cells. This method provides an efficient model for taste research, including taste bud development, regeneration, and transplantation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Seung-Jun Lee, Ka-Hwa Kim, Dong-Joon Lee, Pyunggang Kim, Jinah Park, Seong-Jin Kim, Han-Sung Jung
Summary: Spermatogonial stem cell (SSC) self-renewal is regulated by reciprocal interactions between Sertoli cells and SSCs in the testis. The study focused on the mechanism by which MAST4 in Sertoli cells transmits the signal and regulates SSCs, especially cell cycle regulation. The results demonstrate that MAST4 plays a pivotal role in maintaining the cell cycle of SSCs via the CDK2-PLZF interaction.
CELL PROLIFERATION
(2023)
Article
Dentistry, Oral Surgery & Medicine
E. -j. Kim, H. -y. Kim, L. Li, Q. Tang, K. -h. Kim, H. Ohshima, H. -s. Jung
Summary: The enamel knot (EK) is a cluster of nondividing epithelial cells that acts as a signaling center in tooth germs. This study analyzed the cellular mechanisms in the EK related to bone morphogenetic protein (Bmp) and compared two species with different cuspal patterning. The findings suggest that the cellular mechanisms in the EK, such as cell proliferation and apoptosis, are associated with Bmp4 and play a crucial role in tooth morphogenesis.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Jaehan Park, Narae Jung, Dong-Joon Lee, Seunghan Oh, Sungtae Kim, Sung-Won Cho, Jong-Eun Kim, Hong Seok Moon, Young-Bum Park
Summary: This study aimed to enhance the osteogenic ability of a dual scaffold complex and determine the optimal concentration of growth factors (GF) for new bone formation in guided bone regeneration (GBR). The experimental group treated with BMP-2 0.5 mg/ml and FGF-2 1.0 mg/ml showed continuous formation of new bone and significantly higher new bone formation compared to the control group. Additionally, new bone formation was significantly higher at 8 weeks compared to 2 weeks and 4 weeks. The novel GBR method with BMP-2 applied to the membrane is effective for bone regeneration, and the dual scaffold complex has advantages for bone regeneration and maintenance.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jong-Min Lee, Tae-Yang Lim, Sang-Bin Oh, Seung-Jun Lee, Yun Soo Bae, Han-Sung Jung
Summary: The localization of Ahnak gene is abnormal in the kidney and ureter, which is associated with the imbalance of calcium homeostasis, leading to nonobstructive hydronephrosis. Ahnak plays a crucial role in the development of the kidney and ureter, and in maintaining the function of the urinary system.
CELL AND BIOSCIENCE
(2023)
Article
Engineering, Biomedical
Narae Jung, Jaehan Park, Sang-Hyun Park, Seunghan Oh, Sungtae Kim, Sung-Won Cho, Jong-Eun Kim, Hong Seok Moon, Young-Bum Park
Summary: This study examines the reinforcement effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) applied to collagen membranes in guided bone regeneration. Results show that the combination of collagen membranes with rhBMP-2 and biphasic calcium phosphate (BCP) significantly enhances bone formation rates. The 2-week healing period yields lower bone formation compared to 4 and 8 weeks.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ahmed Elhakim, Sunil Kim, Su-Jung Shin, Han-Sung Jung, Euiseong Kim
Summary: In this study, the authors investigated the effect of icariin on reducing root resorption and promoting functional healing of denuded roots in a rat model of tooth replantation. The results showed that icariin had a dose-dependent inhibitory effect on the differentiation of osteoclasts and enhanced osteogenic activity in stem cells. Micro-CT imaging and histological analysis confirmed the effectiveness of icariin in reducing root resorption and improving tooth retention and survival.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Cell Biology
Ka-Hwa Kim, Eun-Jung Kim, Hyun-Yi Kim, Shujin Li, Han-Sung Jung
Summary: Tooth development relies on the interactions between dental epithelium and dental mesenchyme. In this study, hiPSC-derived human ameloblast organoids (hAOs) were cultured using a 3D system. hAOs exhibited similar properties to ameloblasts and showed enamel formation in response to calcium and mineralization in interaction with dental mesenchyme. Additionally, hAOs showed osteogenic and odontogenic differentiation potential and demonstrated tooth regenerative potential in interaction with mouse dental mesenchyme. This research provides insights into hiPSC-derived dental source and highlights the potential of hAOs for tooth regeneration and study of dental diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anish Ashok Adpaikar, Jong-Min Lee, Dong-Joon Lee, Hye-Yeon Cho, Hayato Ohshima, Seok Jun Moon, Han-Sung Jung
Summary: Taste receptor cells can dedifferentiate and acquire stem/progenitor cell-like states after nerve injury, leading to enhanced taste bud regeneration. This finding suggests that differentiated taste receptor cells have the potential to enter the cell cycle and contribute to taste bud renewal. These dedifferentiated cells, along with resident stem/progenitor cells, play a crucial role in the regeneration of taste buds following nerve injury.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Cell & Tissue Engineering
Shujin Li, Dong-Joon Lee, Hyun-Yi Kim, Hidemitsu Harada, Young-Soo Jung, Han-Sung Jung
Summary: Cancer initiation and progression are closely related to the interaction between cancer cells and the surrounding stroma. This study focuses on the role of collagen-rich extracellular matrix (ECM) in the invasion of ameloblastoma (AM), a representative odontogenic epithelial tumor. Transcriptomic analysis showed up-regulation of ECM- and epithelial-to-mesenchymal transition (EMT)-related genes in AM compared to the ameloblastoma cell line. Tumoroid forming analysis demonstrated the importance of collagen-rich ECM in AM progression, particularly in aggressive growth patterns and collective invasion.
INTERNATIONAL JOURNAL OF STEM CELLS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Tomoaki Komada, Keisuke Mitomo, Takatoshi Ikarashi, Masaki Shimono, Han-Sung Jung, Takashi Muramatsu
Summary: This study used a mouse in situ model to investigate the histological healing process of regenerative endodontic cell-homing procedures. It was found that periodontal ligament cells and vascular endothelial cells grew into the root canals from the apex, replaced the blood clots, and participated in the formation of cementum-like tissues with angiogenesis.
FRONTIERS IN DENTAL MEDICINE
(2022)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)
