Article
Veterinary Sciences
Dawei Wei, Jiupan Zhang, Sayed Haidar Abbas Raza, Yaping Song, Chao Jiang, Xiaoyu Song, Hao Wu, Mashael Alhumaidi Alotaibi, Raed Albiheyri, Majid Al-Zahrani, Raafat T. M. Makhlof, Mohammad A. Alsaad, Sameh A. Abdelnour, Guobo Quan
Summary: This study aims to explore the functional role of Myoz2 in myoblast differentiation and identify the potential factors involved in promoter transcriptional regulation of Myoz2. The results showed that Myoz2 plays an important role in muscle growth and differentiation. Knockdown of Myoz2 inhibited myoblast differentiation and negatively affected the expression of certain genes. Through a series of experiments, the researchers identified MyoD and MyoG as important factors in the core promoter region of Myoz2.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Emanuela Stampone, Debora Bencivenga, Clementina Barone, Marilena Di Finizio, Fulvio Della Ragione, Adriana Borriello
Summary: The p57(Kip2) protein functions as an inhibitor in the nucleus, with mutations affecting its cellular localization and potentially contributing to various genetic diseases. The C-terminal RKRLR sequence is crucial for nuclear entry of p57(Kip2).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
A. Kahsay, E. Rodriguez-Marquez, A. Lopez-Perez, A. Hornblad, J. von Hofsten
Summary: By analyzing gene expression changes in pax3a/pax3b double mutant zebrafish, we found that PAX3 transcription factors play an important role in the malignant transformation of fusion negative rhabdomyosarcoma. Specifically, PAX3 transcription factors regulate several key genes in the RAS/MAPK signaling pathway.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Yunqian Yin, Genghua Chen, Zetong Lin, Danlu Zhang, Wujian Lin, Wen Luo
Summary: Natural antisense transcripts (NATs) are endogenous RNAs opposite to sense transcripts, and they can significantly contribute to regulating various biological processes through multiple epigenetic mechanisms. Our analysis of third-generation full-length transcriptome sequencing data revealed that NATs represented a significant portion of the lncRNA, accounting for up to 30.19%-33.35%. The expression of NATs correlated with myoblast differentiation, and genes expressing NATs were mainly involved in RNA synthesis, protein transport, and cell cycle.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Xiaoxu Shen, Can Cui, Shuyue Tang, Shunshun Han, Yao Zhang, Lu Xia, Bo Tan, Menggen Ma, Houyang Kang, Jie Yu, Qing Zhu, Huadong Yin
Summary: CircGPD2, a muscle-specific circRNA, acts positively in the proliferation and differentiation of myoblasts by releasing the inhibition effect of miR-203a on c-JUN and MEF2C. The myogenic regulatory factor MyoG enhances circGPD2 expression through the E-box element. Knockdown of circGPD2 results in decreased breast muscle mass in chicks.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Agriculture, Multidisciplinary
Ping Li, Xiaoyu Zhang, Liangliang Tian, Yahao Zhao, Yunqin Yan, Shuang Li, Shufeng Li, Huili Tong
Summary: This study investigated the role of vitamin C (VC) in muscle development and identified the molecular mechanism behind it. The researchers found that VC upregulated the expression of specific proteins and promoted muscle cell differentiation and injury repair. These findings provide a theoretical basis for understanding the regulation of muscle development by VC and for the development of nutritional supplements and therapeutic drugs for muscle diseases.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Cell Biology
Benjamin Gellhaus, Kai O. Boeker, Marlene Gsaenger, Eyck Rodenwaldt, Marc A. Hueser, Arndt F. Schilling, Dominik Saul
Summary: Sarcopenia, a prevalent condition among the elderly, has significant socioeconomic importance due to its association with falls, hospitalization, increased healthcare costs, and diminished quality of life. The transcription factor FOXO3 plays a crucial role in muscle equilibrium and can upregulate the expression of Fbxo32, a key driver of muscle protein degradation. This study explored the hypothesis that targeting FOXO3 could regulate this detrimental pathway.
Article
Biochemistry & Molecular Biology
Mengxun Li, Quan Liu, Su Xie, Chong Fu, Jiaxuan Li, Cheng Tian, Xin Li, Changchun Li
Summary: In this study, it was found that the lncRNA TCONS_00323213 is upregulated during porcine skeletal muscle satellite cell (PSC) differentiation and plays a role in the proliferation and differentiation of PSC. The interaction between TCONS_00323213 and PKNOX2 promotes the expression of MyoG and enhances PSC differentiation. This finding provides insights into the molecular mechanisms by which lncRNAs regulate porcine myogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Carlos Camacho-Macorra, Marcos Sintes, Noemi Tabanera, Irene Grasa, Paola Bovolenta, Marcos J. Cardozo
Summary: Hedgehog (Hh) signaling pathway is highly regulated and mutations in its components can lead to a variety of congenital malformations. Mosmo, a modulator of the Hh pathway, plays a role in down-modulating pathway activation. Studies in zebrafish embryos suggest that MOSMO may be a candidate gene for uncharacterized forms of human congenital craniofacial malformations.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Carlos Palma-Flores, Paola B. Zarate-Segura, J. Manuel Hernandez-Hernandez, Sergio de los Santos, Andrea S. Tejeda-Gomez, Luis Javier Cano-Martinez, Patricia Canto, Jorge Omar Garcia-Rebollar, Ramon M. Coral-Vazquez
Summary: This study demonstrates that (-)-epicatechin upregulates miRNAs involved in muscle adaptation, induces muscle hypertrophy, and increases the level of myogenic proteins.
Article
Developmental Biology
Hong-Lan Song, Tai-Hang Liu, Yong-Heng Wang, Fang-Fang Li, Ling-Ling Ruan, Enoch Appiah Adu-Gyamfi, Si-Chen Hu, Xue-Mei Chen, Yu-Bin Ding, Li-Juan Fu
Summary: The study revealed that proper expression of P57 at cytotrophoblast differentiation nodes plays a significant role in trophoblast syncytialization.
Article
Developmental Biology
Philipp M. Tschaikner, Dominik Regele, Ruth Roeck, Willi Salvenmoser, Dirk Meyer, Michel Bouvier, Stephan Geley, Eduard Stefan, Pia Aanstad
Summary: In this study, Gpr161 was found to play a crucial role in negatively regulating the Hh signaling pathway by activating PKA to inhibit the expression of Hh target genes. Additionally, PKA phosphorylation-deficient forms of Gpr161 were shown to increase sensitivity to Shh, resulting in excessive high-level Hh signaling. These findings suggest that PKA-mediated phosphorylation of Gpr161 may provide a mechanism for fine-tuning Gpr161 ciliary localization and PKA activity.
Article
Biology
Zhenzhen Xiong, Mengni Wang, Shanshan You, Xiaoyan Chen, Jiangguo Lin, Jianhua Wu, Xiaozhong Shi
Summary: In this study, we identified the mechanism regulating the dynamic expression of Tceal7 during skeletal muscle regeneration, and demonstrated the interaction between the triple complex of Mef2c, Creb1, and Myod and the Mef2#3-CRE#3-E#4 motifs in the Tceal7 promoter, which drives Tceal7 expression.
Article
Biotechnology & Applied Microbiology
Ping Wu, Pengzheng Yong, Zhanxiong Zhang, Rui Xu, Renjie Shang, Jun Shi, Jianshe Zhang, Pengpeng Bi, Elizabeth Chen, Shaojun Du
Summary: Research indicates that in zebrafish, slow-twitch muscle fibers remain mononucleated while fast-twitch fibers can fuse to form multinucleated fibers. Myomixer plays a crucial role in fusion of specific muscle types, and its genetic deletion leads to severe myopathy and uncontrolled adipose infiltration in both muscle tissues.
MARINE BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jian Heng, Boyang Shi, Jia-Yi Zhou, Yifan Zhang, Dongyuan Ma, Yun-Gui Yang, Feng Liu
Summary: Cpeb1b-mediated cytoplasmic polyadenylation plays a crucial role in hematopoietic stem and progenitor cell development by regulating Hedgehog signaling. Cpeb1b controls hemogenic endothelium specification through the Hedgehog-Vegf-Notch axis, leading to the production of HSPCs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Developmental Biology
Hoi Ting A. Hau, Oluwaseun Ogundele, Andrew H. Hibbert, Clinton A. L. Monfries, Katherine Exelby, Natalie J. Wood, Jessica Nevarez-Mejia, M. Alejandra Carbajal, Roland A. Fleck, Maria Dermit, Faraz K. Mardakheh, Victoria C. Williams-Ward, Tapan G. Pipalia, Maria R. Conte, Simon M. Hughes
Article
Developmental Biology
Daniel P. S. Osborn, Kuoyu Li, Stephen J. Cutty, Andrew C. Nelson, Fiona C. Wardle, Yaniv Hinits, Simon M. Hughes
Article
Biology
Massimo Ganassi, Sara Badodi, Kees Wanders, Peter S. Zammit, Simon M. Hughes
Article
Genetics & Heredity
Daniel Peter Sayer Osborn, Leila Emrahi, Joshua Clayton, Mehrnoush Toufan Tabrizi, Alex Yui Bong Wan, Reza Maroofian, Mohammad Yazdchi, Michael Leon Enrique Garcia, Hamid Galehdari, Camila Hesse, Gholamreza Shariati, Neda Mazaheri, Alireza Sedaghat, Hayley Goullee, Nigel Laing, Yalda Jamshidi, Homa Tajsharghi
Summary: This study identified genetic causes of recessive familial cardiomyopathy using exome sequencing. The results suggest that homozygous MYL3 loss-of-function variants may lead to the development of recessive cardiomyopathy and sudden cardiac death.
GENETICS IN MEDICINE
(2021)
Article
Multidisciplinary Sciences
Jeffrey J. Kelu, Tapan G. Pipalia, Simon M. Hughes
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Developmental Biology
Duvaraka Kula-Alwar, Michael S. Marber, Simon M. Hughes, Yaniv Hinits
Summary: During heart formation, significant growth and morphogenesis occur, with cell proliferation, cardiomyocyte addition, and hypertrophy playing key roles in ventricle growth. Loss of Mef2c activity affects cardiomyocyte addition and differentiation, leading to a smaller ventricle at early stages but recovery in later development.
DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Michael Attwaters, Simon M. Hughes
Summary: Skeletal muscle can grow in three ways: by generating new syncytial fibers, adding nuclei from muscle stem cells to existing fibers, or increasing cytoplasmic volume/nucleus. Evidence suggests that the latter two processes contribute to exercise-induced growth. Fiber growth requires an increase in sarcolemmal surface area and cytoplasmic volume at different rates.
Article
Multidisciplinary Sciences
Gulsum Kayman Kurekci, Ecem Kural Mangit, Cansu Koyunlar, Seyda Unsal, Berk Saglam, Bora Ergin, Merve Gizer, Ismail Uyanik, Niloufar Boustanabadimaralan Duz, Petek Korkusuz, Beril Talim, Nuhan Purali, Simon M. Hughes, Pervin R. Dincer
Summary: The study found that zebrafish have two desmin paralogs, knockout of these genes did not cause muscle phenotype defects but altered calcium flux in myofibres.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Nainita Roy, Swetha Sundar, Malini Pillai, Farah Patell-Socha, Sravya Ganesh, Ajoy Aloysius, Mohammed Rumman, Hardik Gala, Simon M. Hughes, Peter S. Zammit, Jyotsna Dhawan
Summary: The balance of mRNA turnover versus utilization varies in distinct cellular states, with Fmrp playing a key role in muscle tissue homeostasis and Dcp1a playing a crucial role in activation and proliferation processes. The reciprocal expression of Fmrp and Dcp1a puncta suggests their distinct functions in muscle stem cell quiescence, proliferation, and differentiation.
Article
Biology
Simon M. Hughes, Roberta C. Escaleira, Kees Wanders, Jana Koth, David G. Wilkinson, Qiling Xu
Summary: The Musclebow2 method in zebrafish reveals stem cell diversity during skeletal myogenesis, indicating that most muscle precursor cells contribute to local growth and repair, and suggesting low cellular turnover in the absence of trauma.
Article
Developmental Biology
Hoi-Ting A. Hau, Jeffrey J. Kelu, Julien Ochala, Simon M. Hughes
Summary: Slow myosin heavy chain 1 (Smyhc1) is crucial for early contraction of slow skeletal muscle fibers in zebrafish. Mutant alleles lacking smyhc1 function exhibit motility defects, but regain it as fast muscle fibers develop. However, they still experience slow muscle motility issues until about 1 month of age. Further analysis reveals that mutant slow fibers undergo normal differentiation and migration, but fail to generate large myofibrils and maintain cellular orientation due to maturation defects.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Biology
Massimo Ganassi, Peter S. Zammit, Simon M. Hughes
Summary: The study of MuSCs and their associated myofibres allows exploration of muscle stem cell biology, including the mechanisms underlying muscle formation, maintenance, and repair. The comparison to a different vertebrate system, such as zebrafish, provides a valuable tool for understanding the relevance of MuSC biology to other species, including humans. This comparative system offers insights into the genetic and cellular mechanisms of MuSC biology that can inform aquaculture practices.
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)
