期刊
DEVELOPMENTAL BIOLOGY
卷 344, 期 1, 页码 52-65出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2010.04.013
关键词
Chemotaxis; Fertilization; Flagella; Sperm motility; Ca2+ signaling; Sea urchin sperm
资金
- CONACyT [56660, 49113]
- NIH [R01 HD038082-07A1]
- PAPIIT [IN211907, IN211809]
- Wellcome Trust
Marine invertebrate oocytes establish chemoattractant gradients that guide spermatozoa towards their. source In sea urchin spermatozoa, this relocation requires coordinated motility changes initiated by Ca2+-driven alterations in sperm flagellar curvature We discovered that Lytechmus pictus spermatozoa undergo chemotaxis in response to speract, an egg-derived decapeptide previously noted to stimulate non-chemotactic motility alterations in Strongylocentrotus purpuratus spermatozoa Sperm of both species responded to speract gradients with a sequence of turning episodes that correlate with transient flagellar Ca2+ increases, yet only L pictus spermatozoa accumulated at the gradient source Detailed analysis of sperm behavior revealed that L malts spermatozoa selectively undergo Ca2+ fluctuations while swimming along negative speract gradients while S purpuratus sperm generate Ca2+ fluctuations in a spatially nonselective manner This difference is attributed to the selective suppression of Ca2+ fluctuations of L pictus spermatozoa as they swim towards the source of the chemoattractant gradient This is the first study to compare and characterize the motility components that differ in chemotactic and non-chemotactic spermatozoa Tuning of Ca2+ fluctuations and associated turning episodes to the chemoattractant gradient polarity is a central feature of sea urchin sperm chemotaxis and may be a feature of sperm chemotaxis in general. (C) 2010 Elsevier Inc All rights reserved
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