Article
Multidisciplinary Sciences
Jian Tu, Zijun Huo, Yao Yu, Dandan Zhu, An Xu, Mo-Fan Huang, Ruifeng Hu, Ruoyu Wang, Julian A. Gingold, Yi-Hung Chen, Kuang-Lei Tsai, Nicolas R. Forcioli-Conti, Sarah X. L. Huang, Thomas R. Webb, Jie Su, Danielle A. Bazer, Peilin Jia, Jason T. Yustein, Lisa L. Wang, Mien-Chie Hung, Zhongming Zhao, Chad D. Huff, Jingnan Shen, Ruiying Zhao, Dung-Fang Lee
Summary: The study reveals that the spliceosome is an up-regulated target in RB1-mutant cells responding to oncogenic stress, and pRB and E2F3a coregulate spliceosomal gene expression, affecting cell proliferation and tumorigenesis. These findings suggest that the spliceosomal machinery could be a potential therapeutic vulnerability for pRB-deficient cancers.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Veterinary Sciences
Ahmed M. Nagy, Swanand R. Sathe, Attia H. Atta, Abdel Mohsen M. Hammam, Walter H. Hsu
Summary: The study found that mRNA and protein expressions of PRA were significantly higher than PRB in the term equine placenta, indicating that PRA is the dominant isoform expressed in this stage of pregnancy.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Zhe Jiang, Huiqin Li, Stephanie A. Schroer, Veronique Voisin, Youngjun Ju, Marek Pacal, Natalie Erdmann, Wei Shi, Philip E. D. Chung, Tao Deng, Nien-Jung Chen, Giovanni Ciavarra, Alessandro Datti, Tak W. Mak, Lea Harrington, Frederick A. Dick, Gary D. Bader, Rod Bremner, Minna Woo, Eldad Zacksenhaus
Summary: By knocking out phosphorylation sites of pRb in mice, researchers found that this modification does not affect embryonic and early development, but leads to premature aging phenotypes such as infertility, kyphosis, and diabetes. These results have important implications for cell cycle regulation, diabetes treatment, and longevity research.
Review
Oncology
Maria Lopez-Ansio, Pablo Ramos-Garcia, Miguel Angel Gonzalez-Moles
Summary: Oral cancer has a high incidence globally, with OSCC being the most common type. Loss of pRb function is associated with improved survival in patients with OSCC. This systematic review and meta-analysis provide evidence-based results supporting this association.
Article
Cell Biology
Yinjuan Wang, Hao Ming, Leqian Yu, Jie Li, Linkai Zhu, Hai-Xi Sun, Carlos A. Pinzon-Arteaga, Jun Wu, Zongliang Jiang
Summary: A chemical cocktail enables the derivation and long-term culture of bovine trophoblast stem cells, which possess the potential to differentiate into mature trophoblast cells and exhibit similar characteristics to trophectoderm cells from early bovine embryos. These bovine TSCs provide a valuable model to study bovine placentation and early pregnancy failure.
Article
Cell Biology
Badri Krishnan, Takaaki Yasuhara, Purva Rumde, Marcello Stanzione, Chenyue Lu, Hanjun Lee, Michael S. Lawrence, Lee Zou, Linda T. Nieman, Ioannis Sanidas, Nicholas J. Dyson
Summary: RB restricts G1/S progression by inhibiting E2F, but also affects chromosome architecture and cellular state. Expression of RB and G1 arrest leads to visible changes in chromosome structure, which are related to RB activity, specific isoforms of monophosphorylated RB, and known RB-associated activities. Delt ΔCK-RB alters physical interactions between genomic loci, but these changes are not affected by dominant-negative DN.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Acoustics
D. Cui, Y. Liu, X. Jiang, C. Ding, L. C. Poon, H. Wang, H. Yang
Summary: This study found the presence of ACE2- and TMPRSS2-positive cells in the human trophectoderm and placenta in all three trimesters of pregnancy, suggesting the possibility of SARS-CoV-2 spreading through the placenta and causing intrauterine fetal infection.
ULTRASOUND IN OBSTETRICS & GYNECOLOGY
(2021)
Article
Environmental Sciences
Jun Bai, Siwei Deng, Huiyang Fu, Qing Yang, Fazheng Ren, Shenming Zeng, Zhaohui Chen, Ying Yang, Zhenlong Wu
Summary: This study investigated the harmful effects and toxicological mechanism of Chlorpyrifos (CPF) in porcine trophectoderm (pTr) cells and the placenta of female mice during pregnancy. The findings showed that CPF reduced cell viability and increased lactate dehydrogenase (LDH) release in pTr cells. Pregnant mice exposed to CPF also experienced decreased maternal, fetal, and placental weights, indicating reproductive toxicity. Additionally, CPF induced apoptosis in pTr cells and the placenta of female mice, accompanied by depolarization of mitochondrial membrane potential (MMP) and increased reactive oxygen species (ROS) levels. The study further revealed that CPF-induced mitochondrial apoptosis is mediated by the MAPK signaling pathway. Inhibition of the ERK/MAPK signaling pathway alleviated CPF-induced oxidative stress, barrier dysfunction, and mitochondrial apoptosis. These findings suggest that early pregnancy exposure to CPF may pose a potential risk factor for placental formation and function in both humans and animals.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Vaibhav Chand, Xiubei Liao, Grace Guzman, Elizaveta Benevolenskaya, Pradip Raychaudhuri
Summary: This article investigates the inactivation mechanism of retinoblastoma protein (RB1) in hepatocellular carcinoma (HCC). The study finds that overexpressed FOXM1 plays a critical role in HCC by binding to RB1 and DNMT3B to repress the expression of FOXO1, leading to the decrease in CDK-inhibitors and subsequent phosphorylation and inactivation of RB1. Inhibition of FOXM1 results in increased expression of FOXO1, activation of RB1, and senescence of HCC cells.
Article
Genetics & Heredity
Yajun Liu, Yi Zhang, Shiwen Li, Jinquan Cui
Summary: This study reanalyzed single-cell RNA-seq data from post-implantation embryos derived from different sources, identifying different subtypes of trophoblast cells and their characteristic markers, as well as investigating the gene expression patterns of trophoblast-specific transcription factors in different models.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2021)
Article
Obstetrics & Gynecology
Eric Rhon-Calderon, Cassidy Hemphill, Lisa Vrooman, Casey Rosier, Yemin Lan, Teri Ord, Christos Coutifaris, Monica Mainigi, Richard Schultz, Marisa Bartolomei
Summary: TEBx affects the health of the placenta and embryo during early development, with some changes improving later in development and others worsening, leading to metabolic changes in adult offspring.
HUMAN REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Josipa Marin Lovric, Natalija Filipovic, Ljubo Znaor, Anita Rancic, Josko Petricevic, Nenad Kunac, Violeta Soljic, Mirna Saraga-Babic, Katarina Vukojevic
Summary: This study analyzed the expression patterns of markers p19, Ki-67, MSX1, MSX2, PDL1, pRB, and CYCLINA2 in developing and postnatal human eye tissues, retinoblastoma, and uveal melanomas. The expression differences of these markers correlated with the origin and stage of cell differentiation of the tissue samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, General & Internal
Guglielmo Stabile, Maria Sole Scalia, Tamara Stampalija, Matteo Bruno, Antonio Simone Lagana, Andrea Sartore, Alessandro Mangogna, Stefania Carlucci
Summary: Chorangiocarcinoma is a rare and often misdiagnosed placental neoplasm with distinct morphological features. This systematic review examines the entity and biology of chorangiocarcinoma through literature analysis. Eight case reports were analyzed, highlighting macroscopic and microscopic characteristics, immunostaining results, and outcomes for both the mother and fetus. The real prevalence of this pathology may be underestimated due to a lack of knowledge about its histology and clinical course.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Tanja Jankovic-Karasoulos, Dale McAninch, Clare Dixon, Shalem Y. -L. Leemaqz, Maxime Francois, Wayne R. Leifert, Dylan McCullough, Carmela Ricciardelli, Claire T. Roberts, Tina Bianco-Miotto
Summary: The study revealed that high zinc intake can result in decreased proliferation and viability of trophoblast cells, as well as increased oxidative stress levels. Further research is needed to understand the mechanisms behind these effects.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Kun Li, Fengmei Han, Yanping Wu, Xue Wang
Summary: The study identified that miR-340 is highly expressed in retinoblastoma, enhancing cell proliferation, migration, and invasion by regulating WIF1. Moreover, multiple miRNAs and genes were identified that may contribute to a better understanding of the pathogenesis of the disease.
ONCOTARGETS AND THERAPY
(2021)
Article
Cell Biology
Brandon N. Nicolay, Paul S. Danielian, Filippos Kottakis, John D. Lapek, Ioannis Sanidas, Wayne O. Miles, Mantre Dehnad, Katrin Tschoep, Jessica J. Gierut, Amity L. Manning, Robert Morris, Kevin Haigis, Nabeel Bardeesy, Jacqueline A. Lees, Wilhelm Haas, Nicholas J. Dyson
GENES & DEVELOPMENT
(2015)
Article
Cell Biology
Paul S. Danielian, Rex A. Hess, Jacqueline A. Lees
Article
Multidisciplinary Sciences
Munemasa Mori, Renin Hazan, Paul S. Danielian, John E. Mahoney, Huijun Li, Jining Lu, Emily S. Miller, Xueliang Zhu, Jacqueline A. Lees, Wellington V. Cardoso
NATURE COMMUNICATIONS
(2017)
Article
Cell Biology
Christopher L. Sansam, Nelly M. Cruz, Paul S. Danielian, Adam Amsterdam, Melissa L. Lau, Nancy Hopkins, Jacqueline A. Lees
GENES & DEVELOPMENT
(2010)
Article
Multidisciplinary Sciences
Eliezer Calo, Jose A. Quintero-Estades, Paul S. Danielian, Simona Nedelcu, Seth D. Berman, Jacqueline A. Lees
Article
Biochemistry & Molecular Biology
P. S. Danielian, L. B. Friesenhahn, A. M. Faust, J. C. West, A. M. Caron, R. T. Bronson, J. A. Lees
Article
Biochemistry & Molecular Biology
A. S. Landman, P. S. Danielian, J. A. Lees
Article
Multidisciplinary Sciences
Seth D. Berman, Eliezer Calo, Allison S. Landman, Paul S. Danielian, Emily S. Miller, Julie C. West, Borel Djouedjong Fonhoue, Alicia Caron, Roderick Bronson, Mary L. Bouxsein, Siddhartha Mukherjee, Jacqueline A. Lees
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2008)
Article
Multidisciplinary Sciences
Seth D. Berman, Julie C. West, Paul S. Danielian, Alicia M. Caron, James R. Stone, Jacqueline A. Lees
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2009)
Article
Cell Biology
Renin Hazan, Munemasa Mori, Paul S. Danielian, Vincent J. Guen, Seth M. Rubin, Wellington Cardoso, Jacqueline A. Lees
Summary: The multiciliated cells require E2F4 to associate with components of the centriole replication machinery for their cytoplasmic roles in multiciliogenesis. The N-terminal domain of E2F4 is sufficient to mediate its cytoplasmic role in multiciliogenesis by binding to Deup1 and SAS6. The ability of E2F4 and E2F5 to recruit Deup1 and/or SAS6, and facilitate centriole replication, contributes to their shared roles in multiciliogenesis.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Oncology
Molly M. M. Wilson, Paul S. S. Danielian, Griffin Salus, Roberta Ferretti, Charles A. A. Whittaker, Jacqueline A. A. Lees
Summary: BMI1 is involved in the self-renewal and proliferation of adult stem cells and tumors, but in cutaneous melanoma, it promotes epithelial-mesenchymal transition and metastasis while having little effect on proliferation or primary tumor growth. Deletion of melanocyte-specific Bmi1 in mice leads to premature hair greying and gradual loss of melanocytes, and depilation accelerates the depletion of melanocyte stem cells, suggesting a protective role of BMI1 against stress in these cells.
PIGMENT CELL & MELANOMA RESEARCH
(2023)
Article
Developmental Biology
Paul S. Danielian, Carla F. Bender Kim, Alicia M. Caron, Eliza Vasile, Roderick T. Bronson, Jacqueline A. Lees
DEVELOPMENTAL BIOLOGY
(2007)
Article
Cell Biology
Christopher L. Sansam, Jennifer L. Shepard, Kevin Lai, Alessandra Ianari, Paul S. Danielian, Adam Amsterdam, Nancy Hopkins, Jacqueline A. Lees
GENES & DEVELOPMENT
(2006)
Article
Developmental Biology
KL Ligon, Y Echelard, S Assimacopoulos, PS Danielian, S Kaing, EA Grove, AP McMahon, DH Rowitch
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)