Article
Cell & Tissue Engineering
Le Phuong Hoang Anh, Ken Nishimura, Akihiro Kuno, Nguyen Thuy Linh, Tetsuo Kato, Manami Ohtaka, Mahito Nakanishi, Eiji Sugihara, Taka-Aki Sato, Yohei Hayashi, Aya Fukuda, Koji Hisatake
Summary: Somatic cell reprogramming proceeds through a series of events to generate induced pluripotent stem cells (iPSCs). In this study, the researchers found that the downregulation of Osr2 is crucial for efficient reprogramming by using a cell-based model. Osr2 acts as a novel EMT regulator and its downregulation diminishes TGF-beta signaling and activates Wnt signaling, promoting MET and acquiring pluripotency.
Article
Cell Biology
Ivana Bukova, Katarzyna Izabela Szczerkowska, Michaela Prochazkova, Inken M. Beck, Jan Prochazka, Radislav Sedlacek
Summary: The study found that in the WIZ knockout mouse model, defective embryos exhibited abnormalities in multiple aspects during development, particularly in the craniofacial region, such as shorter snout, cleft palate, and cleft eyelids. These results suggest that WIZ plays an important role in craniofacial development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Chengyi Fu, Shu Lou, Guirong Zhu, Liwen Fan, Xin Yu, Weihao Zhu, Lan Ma, Lin Wang, Yongchu Pan
Summary: The Let-7c-5p-PIGA and miR-193a-3p-TGFB2 networks may be involved in the development of NSCL/P, with a negative correlation between miRNA and mRNA expression detected in NSCL/P lip tissues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Anatomy & Morphology
Susanne Thuemecke, Reinhard Schroeder
Summary: The study revealed that in Tribolium, drm acts independently of other odd-family members in the formation of appendages and hindgut, while drm and sob are identified as further markers for segment borders in the appendages. Additionally, the regulatory interrelationship among the odd genes is found to differ between Tribolium and Drosophila.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Genetics & Heredity
Baiba Lace, Sander Pajusalu, Diana Livcane, Ieva Grinfelde, Ilze Akota, Ieva Maulina, Biruta Barkane, Janis Stavusis, Inna Inashkina
Summary: Craniofacial morphogenesis is a complex process that can be affected by genetic and environmental factors, leading to orofacial clefts. A study using whole genome sequencing found rare disease variants in genes associated with isolated cleft palate in 17% of patients. These findings suggest that newborns with orofacial clefts should be offered genetic testing, especially for known cleft lip and palate genes.
FRONTIERS IN GENETICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Farid Ghazi Vakili, Masoud Nouri-Vaskeh, Elham Eghbali, Shahin Abdollahi Fakhim
Summary: This study compared the developmental characteristics of bony nasopharyngeal (NP) in children with cleft palate (CP) and non-cleft subjects. The results showed that patients with repaired CP had some differences in NP bone structure, while there were no significant differences in the development of NP among patients with unilateral cleft lip and palate (CLP) or bilateral CLP compared to normal subjects.
JOURNAL OF STOMATOLOGY ORAL AND MAXILLOFACIAL SURGERY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Julie Van Dyck, Giacomo Begnoni, Guy Willems, Annouschka Laenen, Patrick Thevissen, Anna Verdonck, Maria Cadenas de Llano-Perula
Summary: The study found that dental development in unilateral cleft lip and palate patients was more delayed compared to the control group, with females showing the most significant delay under 13 years old and males under 12 years old. In the cleft group, tooth development in the maxilla was more delayed than in the mandible, but there was no evidence of slower development on the affected side compared to the non-affected side.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Developmental Biology
Mirvat El-Sibai, Joelle El Hajj, Maria Al Haddad, Nada El Baba, Mounir Al Saneh, Wassim Daoud Khatoun, Raphael Helaers, Miikka Vikkula, Oula El Atat, Joseph Sabbagh, Naji Abou Chebel, Michella Ghassibe-Sabbagh
Summary: Cleft lip and palate result from the improper migration and assembly of lip buds and palatal shelves during embryogenesis. The dysregulation of Rho GTPases may be correlated with the observed phenotype of cleft lip and palate patient cells. Additionally, patient cells show decreased migration, increased adhesion, and a more elongated phenotype.
CELLS & DEVELOPMENT
(2021)
Article
Dentistry, Oral Surgery & Medicine
Stephan Christian Moehlhenrich, Kristian Kniha, Zuzanna Magnuska, Sachin Chhatwani, Benita Hermanns-Sachweh, Felix Gremse, Frank Hoelzle, Gholamreza Danesh, Ali Modabber
Summary: The study found that autografts, human xenografts, and synthetic bone substitute used for cleft repair have similar effects on subsequent orthodontic tooth movement and associated root resorption.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Otorhinolaryngology
Karim Ahmed Sakran, Shiming Zhang, Nan Chen, Jiayi Yin, Khaled Alkebsi, Sadam Ahmed Elayah, Remsh Khaled Al-Rokhami, Mohammed Qasem Al-Watary, Wael Telha, Maged Al-Aroomi, Bing Shi, Heng Yin, Hanyao Huang
Summary: This study investigated the development of velopharyngeal function (VPF) after primary palatoplasty in cleft palate patients and the factors associated with it. The findings showed that early age of surgery and long-term follow-up were critical factors for confirming normal VPF diagnosis at the first evaluation.
INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY
(2023)
Review
Anatomy & Morphology
Atukorallaya Devi Sewvandini Atukorala, Ravindra Kumar Ratnayake
Summary: Human cleft lip and/or palate (CLP) are congenital abnormalities that can be easily recognized, caused by coordinated activities of neural crest cells and ectodermal epithelial cells. Genetic and environmental factors play significant roles in the progression of this disease. Zebrafish serves as a valuable model organism in identifying genetic and environmental etiological factors of CLP and understanding craniofacial development.
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2021)
Article
Health Care Sciences & Services
Conrad Harrison, Inge Apon, Kenny Ardouin, Chris Sidey-Gibbons, Anne Klassen, Stefan Cano, Karen Wong Riff, Andrea Pusic, Sarah Versnel, Maarten Koudstaal, Alexander C. Allori, Carolyn Rogers-Vizena, Marc C. Swan, Dominic Furniss, Jeremy Rodrigues
Summary: Routine use of patient-reported outcome measures (PROMs) and computerized adaptive tests (CATs) can improve care for surgical conditions. However, most available CATs lack condition-specificity, patient involvement, and clinically relevant score interpretation. This study developed a CAT for the CLEFT-Q PROM, aiming to facilitate its international uptake and provide an open-source framework for CAT development in other surgical conditions.
JOURNAL OF MEDICAL INTERNET RESEARCH
(2023)
Article
Surgery
Zachary D. Zapatero, Anna R. Carlson, Mychajlo S. Kosyk, Christopher L. Kalmar, Jordan W. Swanson, Scott P. Bartlett, Arastoo Vossough, Jesse A. Taylor
Summary: This study aims to evaluate facial suture patency in patients with cleft lip and palate (CL/P) and maxillary hypoplasia. The results show that there is no significant difference in intermaxillary suture fusion rate between CL/P patients and unaffected controls. However, the pathologic absence of the midpalatal suture is more commonly present in CL/P patients with midface hypoplasia.
JOURNAL OF CRANIOFACIAL SURGERY
(2022)
Article
Nutrition & Dietetics
Keisuke Satake, Takenobu Ishii, Taiki Morikawa, Teruo Sakamoto, Yasushi Nishii
Summary: Quercetin, a flavonoid with various pharmacological activities, has been found to prevent TCDD-induced cleft palate by inhibiting CYP1A1 through AhR. This study suggests the potential of quercetin as a preventive measure for cleft palate.
Article
Medicine, General & Internal
Mohamed Zahoor Ul Huqh, Johari Yap Abdullah, Matheel AL-Rawas, Adam Husein, Wan Muhamad Amir W. Ahmad, Nafij Bin Jamayet, Maya Genisa, Mohd Rosli Bin Yahya
Summary: This study predicts the maxillary arch growth for individuals with cleft lip and palate (CLP) using a neural network logistic regression model. The results suggest a strong association between gender, age, and cleft, and the difference in maxillary arch width and length is mainly related to the severity of the cleft and facial growth pattern.
Article
Endocrinology & Metabolism
Han Liu, Jingyue Xu, Rulang Jiang
JOURNAL OF BONE AND MINERAL RESEARCH
(2019)
Article
Biology
Jingyue Xu, Han Liu, Yu Lan, Mike Adam, David E. Clouthier, Steven Potter, Rulang Jiang
Article
Dentistry, Oral Surgery & Medicine
Z. Liu, C. Li, J. Xu, Y. Lan, H. Liu, X. Li, P. Maire, X. Wang, R. Jiang
JOURNAL OF DENTAL RESEARCH
(2019)
Article
Dentistry, Oral Surgery & Medicine
Y. Lan, C. Qin, R. Jiang
JOURNAL OF DENTAL RESEARCH
(2019)
Article
Dentistry, Oral Surgery & Medicine
J. Xu, H. Liu, Y. Lan, J. S. Park, R. Jiang
JOURNAL OF DENTAL RESEARCH
(2020)
Article
Developmental Biology
Chaochang Li, Han Liu, Yueh-Chiang Hu, Yu Lan, Rulang Jiang
Article
Cell Biology
Hye Jung Ihn, Ju Ang Kim, Jiwon Lim, Sang-Hyeon Nam, So Hyeon Hwang, Young Kyung Kim, Jae-Young Kim, Jung-Eun Kim, Eui-Sic Cho, Rulang Jiang, Eui Kyun Park
Summary: Tooth root development involves the interaction of growth factors and transcription factors in Hertwig's epithelial root sheath and dental mesenchyme. Knockout of the Bbx gene in mice results in reduced length of tooth roots and downregulation of Dspp expression during early root formation, suggesting a role for Bbx in tooth root formation.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Anatomy & Morphology
Minghui Yue, Yu Lan, Han Liu, Zhaoming Wu, Toru Imamura, Rulang Jiang
Summary: The study demonstrates that Fgf18 expression in neural crest-derived craniofacial mesenchyme plays a critical role in the development of the mandible and multiple craniofacial bones, while Fgf18 expression in the palatal mesenchyme is dispensable for palatogenesis.
DEVELOPMENTAL DYNAMICS
(2021)
Article
Cell Biology
Jingyue Xu, Han Liu, Yu Lan, Rulang Jiang
Summary: The disruption of FOXF2 gene is associated with cleft palate in humans and mice. Mutations in the Foxf2 gene significantly affect the expression of neighboring genes, particularly Foxq1 and Exoc2. Through gene editing in mice, it was demonstrated that these mutations regulate palatal gene expression, emphasizing the importance of Foxf2 in development and disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Han Liu, Jingyue Xu, Yu Lan, Hee-Woong Lim, Rulang Jiang
Summary: This study investigated the molecular mechanisms of tendon development by generating a Scx(Flag) knockin mouse strain and identifying Scx direct target genes in developing tendon tissues. The results provide novel insights into the role of Scx in tendon development and homeostasis, offering valuable resources for further research on tendon cell differentiation and tissue regeneration. The Scx(Flag) mice present a useful tool for unraveling the molecular mechanisms underlying developmental and disease processes involving Scx.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
J-M Lee, C. Qin, O. H. Chai, Y. Lan, R. Jiang, H-J E. Kwon
Summary: Tooth agenesis is a common birth defect that occurs due to morphogenesis failure in early tooth development. This study shows that Msx1 regulates Wnt signaling by suppressing the expression of Dkk2 and Sfrp2, and that Sostdc1/Wise also plays a role in the odontogenic pathway downstream of Msx1. The findings suggest that modulation of Wnt signaling by Dkk2 and Sostdc1 is crucial for proper tooth morphogenesis.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Cell Biology
Paul P. R. Iyyanar, Zhaoming Wu, Yu Lan, Yueh-Chiang Hu, Rulang Jiang
Summary: The loss of ALX1 function leads to frontonasal dysplasia syndrome FND3, which is characterized by severe facial clefting and microphthalmia. This study generated an Alx1-deletion mouse model using CRISPR/Cas9-mediated genome editing and found that Alx1 is strongly expressed in frontonasal neural crest cells, playing a role in periocular and frontonasal mesenchyme development. Alx1(del/del) embryos showed increased apoptosis in periocular mesenchyme, decreased expression of ocular developmental regulators Pitx2 and Lmxb1, and disrupted frontonasal mesenchyme identity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Paul P. R. Iyyanar, Chuanqi Qin, Nirpesh Adhikari, Han Liu, Yueh-Chiang Hu, Rulang Jiang, Yu Lan
Summary: The origin of the premaxilla bone in mammals has been a topic of debate. Recent studies suggest that it is derived from embryonic maxillary prominences instead of the frontonasal ectome-senchyme as previously thought. This study used genetic labeling techniques in mice to trace the contributions of different embryonic mesenchyme to the premaxillary bone, providing evidence for the developmental origin of the premaxilla.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Jingyue Xu, Paul P. R. Iyyanar, Yu Lan, Rulang Jiang
Summary: Mutations in SHH and other genes in the Hedgehog signaling pathway are associated with holoprosencephaly syndromes and craniofacial anomalies. SHH signaling plays crucial roles in craniofacial morphogenesis, including cell survival, growth and patterning, and organogenesis of various structures. This article summarizes recent advances in understanding the molecular and cellular mechanisms of SHH signaling and its involvement in the formation and patterning of craniofacial structures.
Article
Developmental Biology
Lu Han, Jingyue Xu, Emily Grigg, Megan Slack, Praneet Chaturvedi, Rulang Jiang, Aaron M. Zorn
DEVELOPMENTAL BIOLOGY
(2017)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)