期刊
DEVELOPMENTAL BIOLOGY
卷 323, 期 1, 页码 114-129出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2008.08.007
关键词
Neural crest; Specification; Maintenance; Endothelin; Ednra; Apoptosis; Cell migration
资金
- ANPCyT-Foncyt [PICT10623]
- CIUNT-Foncyt [PICTO UNT 367]
- CONICET [PIP 6278]
- CIUNT [26/D307]
- Millennium Program [ICM P02-050]
- Howard Hughes Medical Institute
- MRC
- BBSRC
- Millennium Program
- MRC [G117/506] Funding Source: UKRI
- Medical Research Council [G117/506] Funding Source: researchfish
The neural crest is induced at the border of the neural plate in a multistep process by signals emanated from the epidermis, neural plate and mesoderm. In this work we show for the first time the existence of a neural crest maintenance step which is dependent on signals released from the mesoderm. We identified Endothelin-1 (Edn1) and its receptor (Ednra) as key players of this signal and we show that Edn1/Ednra signaling is required for maintenance of the neural crest by a dual mechanism of cell specification and cell Survival. We show that: (i) Ednra is expressed in prospective neural crest; (ii) loss-of-function experiments with antisense morpholino or with specific chemical inhibitor suppress the expression of early neural crest markers; (iii) gain-of-function experiments expand the neural crest territory; (iv) epistatic experiments show that Ednra/Edn1 is downstream of the early neural crest gene Msx1 and upstream of the late genes Sox9 and Sox10; and (v) Edn1/Ednra signaling inhibits apoptosis and controls cell specification of the neural crest. Together, our results provide insight on a new role of Edn1/Ednra cell signaling pathway during early neural crest development. (C) 2008 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据