Article
Toxicology
Hitoshi Terui, Yutaka Kimura, Chizu Fujimura, Setsuya Aiba
Summary: This study demonstrated that various haptens can increase the expression of IL-1 alpha and IL-1 beta in human monocyte cell line THP-1, and introduced a stable IL-1 beta reporter cell line THP-G1b for in vitro skin sensitization testing, showing that most haptens can stimulate IL-1 beta reporter activity.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Toxicology
Yutaka Kimura, Chizu Fujimura, Setsuya Aiba
Summary: By modifying the criteria, the number of indeterminate chemicals was reduced and specificity was increased, highlighting the unique advantage of the IL-8 Luc assay.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Pharmacology & Pharmacy
Sorina Andreea Anghel, Rodica Aura Badea, Gabriela Chiritoiu, David Sebastian Patriche, Petruta Ramona Alexandru, Florentina Pena
Summary: GLP-1 secretion plays a crucial role in controlling blood glucose levels. A newly developed cell line allowed for rapid assessment of GLP-1 secretion. The discovery of quercetin as a potent GLP-1 secretagogue opens up possibilities for developing alternative glucose-lowering therapies.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Oncology
Yan Hu, Bing Wang, Ke Yi, Qingjun Lei, Guanghui Wang, Xiaohui Xu
Summary: IRF1 promotes the expression of IFI35 in CRC cells, which is involved in regulating the radiation sensitivity of CRC cells and may be a target for enhancing the effectiveness of radiation therapy in CRC.
CANCER CELL INTERNATIONAL
(2021)
Article
Plant Sciences
Yuan Lin, Hainan Zhao, Magdalena Kotlarz, Jiming Jiang
Summary: Gene expression is regulated by interactions between cis-regulatory DNA elements (CREs) and regulatory proteins. Enhancers play a crucial role in this process, especially in eukaryotes, but little is known about the molecular mechanisms involved in plants. Researchers identified a distal enhancer, CRE beta, which regulates gene expression in response to osmotic pressure, and established a forward genetic screen pipeline to identify trans-regulatory factors associated with CRE beta function and the regulation of the endogenous gene AtDGK7.
Article
Biochemical Research Methods
Eduardo Gonzalez-Grandio, Gozde S. Demirer, Wenhe Ma, Siobhan Brady, Markita P. Landry
Summary: Transcriptional reporter assays are crucial in plant synthetic biology for constructing complex genetic circuits and understanding gene expression control. The dual color luciferase ratiometric reporter system developed in this study offers advantages including speed, cost-effectiveness, and reduced variability.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Analytical
Mu-Shen Chang, Chia-Yi Lee, En-Shuo Liu, Hsuan Chao, Hsin-Yu Wu, Yu-Yen Chang, Yen-Ling Liu, Yu-Tung Chen, Yu-Cheng Su, Yeng-Tseng Wang, Tian-Lu Cheng, Chia-Hung Yen, Cheng-Wei Lin, Hsin-Kai Huang, Wen-Wei Lin
Summary: Researchers have developed a new gene reporter system called mHRP, which uses a cell membrane-expressed HRP to preserve enzymatic activity on the cell surface, avoiding cell lysis and the use of expensive luciferase substrates. The mHRP system provides stable detection signals and has a longer substrate half-life.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Grazyna Laska, Elwira Sieniawska, Magdalena Maciejewska-Turska, Lukasz Swiatek, David S. Pasco, Premalatha Balachandran
Summary: This study aimed to investigate the inhibitory effect of methanolic extracts from Pulsatilla vulgaris Mill. on HeLa cell proliferation by modulating cancer-related signaling pathways. The study also determined the cytotoxicity and chemical composition of P. vulgaris extracts from leaves and roots. The results showed that the root extract of P. vulgaris inhibited 12 signaling pathways in cervical cancer cells, with the most significant inhibition observed for MYC, Notch, Wnt, E2F, Ets, Stat3, Smad, Hdghog, AP-1, and NF-kappa B at a concentration of 40 μg/mL. The methanolic extracts of P. vulgaris induced apoptotic death and disrupted cellular proliferation, differentiation, and progression towards a neoplastic phenotype by altering key signaling molecules necessary for cell cycle progression. Additionally, phytochemical analysis revealed that compounds strongly suppressing the growth and proliferation of HeLa cancer cells in the methanolic extracts of P. vulgaris were mainly triterpenoid saponins accompanied by phenolic acids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Aaiyas Mujawar, Pratham Phadte, Ksenia A. Palkina, Nadezhda M. Markina, Ameena Mohammad, Bhushan L. Thakur, Karen S. Sarkisyan, Anastasia V. Balakireva, Pritha Ray, Ilia Yamplosky, Abhijit De
Summary: This study presents the development of a humanized luciferase imaging reporter based on a mushroom luciferase called Luz. In vitro and in vivo assessments demonstrate that the human-codon-optimized Luz has higher activity than native Luz in cancer cell types. The importance of live cell multiplexed measurement of gene regulatory function using more than two luciferases is suggested to address realistic situations in disease biology.
Article
Biology
Yukiko Miyazaki, Martijn W. Vos, Fiona J. A. Geurten, Pierre Bigeard, Hans Kroeze, Shohei Yoshioka, Mitsuhiro Arisawa, Daniel Ken Inaoka, Valerie Soulard, Koen J. Dechering, Blandine Franke-Fayard, Shinya Miyazaki
Summary: This article presents a versatile Plasmodium falciparum dual reporter line that expresses both a fluorescent protein and NanoLuc under a constitutive promoter. The system can be used to screen for novel anti-malarial drugs effective against multiple stages of the parasite. Through the system, the researchers identified an anti-malarial compound that kills the asexual blood stage parasites.
COMMUNICATIONS BIOLOGY
(2023)
Article
Plant Sciences
Ying Wang, Hongyuan Zhang, Qian Li, Jing Jin, Hao Chen, Yu Zou, Xingguo Huang, Yi Ding
Summary: In this study, the role of lncRNAs in the fertility transition of PTGMS rice lines was investigated through strand-specific RNA sequencing and computational analysis. The results showed that lncRNAs play a critical role in pollen mother cells meiosis, anther and pollen development during the fertility transition. Some lncRNAs were identified as precursors for miRNAs or endogenous target mimics, regulating the expression of miRNAs and mRNAs and contributing to the fertility transition process.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Fisheries
Yao Xu, Xianyun Ren, Yuefeng Cai, Jianjian Lv, Xianliang Meng, Baoquan Gao, Ping Liu, Jian Li
Summary: This study investigated the histological alterations and differentially expressed miRNAs in the hepatopancreas of Portunus trituberculatus exposed to FLR stress. The results showed that FLR stress led to abnormalities in the hepatopancreas structure and alterations in the expression of different miRNAs. miR-263b was found to directly inhibit cytochrome C expression at the cell level. miRNAs play a role in cell apoptosis and immune regulation in crabs.
AQUACULTURE REPORTS
(2023)
Article
Audiology & Speech-Language Pathology
Sang-Yeon Lee, Hyo Soon Yoo, Jin Hee Han, Dae Hee Lee, Sang Soo Park, Myung Hwan Suh, Jun Ho Lee, Seung-Ha Oh, Byung Yoon Choi
Summary: This study describes the interesting audiologic characteristics of LMX1A-associated deafness, suggesting that LMX1A may be associated with dominantly inherited asymmetric hearing loss. By studying new LMX1A variants, the genotypic spectrum of disease-causing variants of LMX1A causing DFNA7 has been expanded.
Article
Biochemistry & Molecular Biology
Florian Rosier, Audrey Brisebarre, Claire Dupuis, Sabrina Baaklini, Denis Puthier, Christine Brun, Lydie C. Pradel, Pascal Rihet, Didier Payen
Summary: Through two genome-wide association studies, the research identified an association between SNP within the CISH gene and death in septic shock patients. Further investigation revealed rs143356980 as a key regulatory candidate that modulates pathways related to death, increasing the risk of mortality in patients with septic shock.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Matthias Kotthoff, Julia Bauer, Franziska Haag, Dietmar Krautwurst
Summary: The study reveals the presence of highly conserved C-terminal amino acid motifs in odorant receptors, which differentiate between class-I and class-II receptors and increase in number during evolution. Experimentation shows that these motifs have differential impacts on the cell surface expression and cAMP signaling of odorant receptors, with some individual amino acids and combinations playing a key role.
Article
Cell Biology
Ana Born-Torrijos, Anush Kosakyan, Sneha Patra, Joana Pimentel-Santos, Brian Panicucci, Justin Tze Ho Chan, Tomas Korytar, Astrid S. Holzer
Summary: In this study, a method was developed to isolate myxozoan proliferative stages from fish blood, achieving high purity and survival rates. Additionally, a rapid cytometric assay was developed for quantitative analysis of these stages. This tool will advance functional research on myxozoans and provide important information for the development of therapeutic strategies against myxozoan diseases.
Article
Biochemistry & Molecular Biology
Ruth Montero, Justin Tze Ho Chan, Bernd Koellner, Roman Kuchta, Jakub Vyslouzil, Peter Podhorec, Astrid Sibylle Holzer, Tomas Korytar
Summary: Poly lactic-co-glycolic acid (PLGA) particles can serve as carriers for drugs and vaccine antigens in fishes, and the immune system of common carp recognizes and reacts to PLGA microparticles. PLGA particles can play a dual role as both antigen and drug carriers in fishes.
Article
Biology
Ruth Montero, Justin Tze Ho Chan, Claudia Mueller, Philip Niclas Just, Sven Ostermann, Margareth overland, Kevin Maisey, Tomas Korytar, Bernd Kollner
Summary: This study demonstrates that the immune system and physiological functions of rainbow trout are regulated seasonally, beyond the influence of photoperiod and water temperature. In winter, the fish's immune response is reduced, but the levels of natural antibodies increase.
Article
Fisheries
Trine Ytrestoyl, Elise Hjelle, Jelena Kolarevic, Harald Takle, Alexander Rebl, Sergey Afanasyev, Aleksei Krasnov, Per Brunsvik, Bendik Fyhn Terjesen
Summary: The production of Atlantic salmon smolts in recirculation aquaculture systems (RAS) is growing, and the use of continuous light has been shown to have positive effects on growth rate. However, continuous light may reduce feed intake and growth during the initial stage in seawater. Fish exposed to continuous light have a larger size at slaughter compared to those given a winter signal. The best performing group is the control group transferred at 100 g.
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2023)
Article
Reproductive Biology
Carolin Lisa Michaela Ludwig, Simon Bohleber, Rebecca Lapp, Alexander Rebl, Eva Katrin Wirth, Martina Langhammer, Ulrich Schweizer, Joachim M. M. Weitzel, Marten Michaelis
Summary: The development and maturation of ovarian follicles is a complex and highly regulated process, which is essential for successful ovulation. The FL1 mouse model, a unique selection experiment for increased female reproductive performance, provides insights into the regulation of folliculogenesis.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Environmental Sciences
Marc-Christopher Hildebrand, Alexander Rebl, Julien Alban Nguinkal, Harry Wilhelm Palm, Bjoern Bassmann
Summary: Fingerlings and juveniles of African catfish were reared under experimental conditions and exposed to different concentrations of Fe-DTPA. Various factors such as growth, behavior, immune response, histopathological changes, and genetic biomarkers were evaluated. The results indicate that Fe-DTPA supplementation at the tested concentrations is relatively harmless for the health and welfare of African catfish.
Article
Biochemistry & Molecular Biology
Doret R. van Muilekom, Bertrand Collet, Henrike Rebl, Kristina Zlatina, Fabio Sarais, Tom Goldammer, Alexander Rebl
Summary: NF-kappa B signaling is controlled by the family of 'inhibitors of NF-kappa B' (I kappa B). Rainbow trout genome contains multiple gene copies coding for different I kappa B proteins, except for two types. The expression levels of these genes vary, suggesting their involvement in immune-regulatory processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Justin T. H. Chan, Amparo Picard-Sanchez, Jovana Majstorovic, Alexander Rebl, Dirk Koczan, Filip Dycka, Astrid S. Holzer, Tomas Korytar
Summary: The presence of pathogen antibodies on red blood cells of infected rainbow trout indicates that red blood cells play a larger role in host immunity than previously appreciated.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Volker Roettgen, Lisa-Maria Tuemmler, Dirk Koczan, Alexander Rebl, Bjoern Kuhla, Jens Vanselow, Anja Baufeld
Summary: This study analyzed the effects of milk replacer feeding on the number and gene expression characteristics of granulosa cells in cattle. The results showed that the number of granulosa cells in puberty was not affected by the number of follicles in the group fed less milk replacer, while in the group fed more milk replacer, the number of granulosa cells increased with the number of follicles. The gene expression analysis revealed that feeding different milk replacers can affect immune response pathways.
Article
Fisheries
Bjoern Bassmann, Lisa Hahn, Alexander Rebl, Lisa Carolina Wenzel, Marc-Christopher Hildebrand, Marieke Verleih, Harry Wilhelm Palm
Summary: This study investigated the effects of different stocking densities (semi-intensive, intensive, and super-intensive) on the growth and welfare of African catfish. The results showed that the semi-intensively stocked fish had the highest weight, while the super-intensively stocked fish had the lowest weight. The management of stocking densities should be carefully considered to avoid external stressors.
Article
Fisheries
Tatyana Gebauer, Radek Gebauer, Petr Cisar, Jan Cerny, Deepali Rahi Roy, Mahyar Zare, Marieke Verleih, Vlastimil Stejskal, Alexander Rebl
Summary: The sensitivity to stress and its impact on immunity are related to a fish's personality. This study found clear differences between shy and bold individuals in a European perch population, based on cognitive tests. Shy individuals exhibited frozen activity and lower explorative behavior, as well as higher cortisol levels. Gene expression analysis showed modest differences between stimulated bold and shy perch, indicating a link between fish personality and immune-defense strategy. This research has implications for future selective breeding programs.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Meeting Abstract
Fisheries
Doret Van Muilekom, Bertrand Collet, Henrike Rebl, Kristina Zlatina, Fabio Sarais, Tom Goldammer, Alexander Rebl
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Fabio Sarais, Ruth Montero, Sven Ostermann, Alexander Rebl, Bernd Koellner, Tom Goldammer
Summary: In this study, the immune response of rainbow trout to bacterial stimulation was examined by analyzing the gene expression patterns of different cell subpopulations in the head kidney. The results provide insights into the regulation of key genes involved in fish immune response to bacterial infection and their diagnostic significance.
Article
Fisheries
Henrike Seibel, Elvis Chikwati, Carsten Schulz, Alexander Rebl
Summary: This study evaluated the welfare of rainbow trout under inadequate husbandry conditions by assessing a diverse range of feeding stress markers. Soybean meal-induced enteritis was observed in rainbow trout, along with changes in blood parameters and gene expression. The findings provide valuable information for monitoring feeding regimens and developing detection devices in aquaculture.
Article
Fisheries
Ratiporn Thongsoi, Siriluk Maskaew, Panumas Puechpon, Benjaporn Noppradit, Napassawan Inaek, Prapaporn Utarabhand, Phanthipha Runsaeng
Summary: In this study, a PPAE gene called FmPPAE1 was cloned and analyzed in banana shrimp. FmPPAE1 plays a vital role in regulating the proPO system, exhibits antibacterial properties, and contributes to the suppression of bacterial proliferation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jishuang Tan, Paizelaiti Sahaer, Huan Li, Wei Han, Hongyan Sun
Summary: Circular RNAs (circRNA) are involved in gene expression and affect cellular processes such as transcription, splicing, and translation by interacting with microRNA and RNA binding proteins. A study identified circDNAJB6 as a circular RNA abundant in chicken macrophages after bacterial infection, and found that circDNAJB6 exacerbates cellular injury and interacts with multiple miRNAs and target genes, potentially impacting autophagy and MAPK signaling pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Rodrigo Guabiraba, Damaris Ribeiro Rodrigues, Paul T. Manna, Melanie Chollot, Vincent Saint-Martin, Sascha Trapp, Marisa Oliveira, Clare E. Bryant, Brian J. Ferguson
Summary: The innate immune response relies on the ability of host cells to detect and respond to microbial nucleic acids. Toll-like receptors (TLRs) play a crucial role in this process by distinguishing self from non-self. This study focused on TLR21, an avian TLR that recognizes bacterial DNA motifs. The findings suggest that avian TLR21 shares similar activation mechanisms to mammalian TLR9, highlighting the conservation of nucleic acid sensing mechanisms across species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Safieh Zeinali, Kate Sutton, Lonneke Vervelde
Summary: Chickens have a unique immune structure characterized by the absence of draining lymph nodes and the presence of well-developed mucosa-associated lymphoid tissue. The structure and development of chicken lymphoid tissues in the intestine, particularly Peyer's patches, have been poorly studied. However, the use of CSF1R-eGFP reporter transgenic chickens has allowed for the visualization and investigation of the development and distribution of these tissues. This research provides insights that could benefit mucosal vaccination strategies and the development of targeted vaccine delivery systems.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Retraction
Fisheries
G. -g. Li, Z. -z. Guo, X. -f. Ma, N. Cao, S. -n. Geng, Y. -q. Zheng, M. -j. Meng, H. -h. Lin, G. Han, G. -j. Du
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jinyue Yang, Peng Zhou, Wanrong Wu, Qingxiang Zhang, Dong Chen, Rui Luo
Summary: This study cloned and characterized the full-length sequence of duck MRE11 (duMRE11) for the first time, and found that this protein plays an important role in the innate immune response of ducks.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Mengjiao Guo, Jiaqi Zhang, Mingtao Li, Xiaorong Zhang, Yantao Wu
Summary: rNLRC3 negatively regulates the NF -KB signaling pathway induced by P. multocida in rabbits by inhibiting the activation of NF -KB, reducing the expression of inflammatory cytokines, and interacting with rTRAF4 and rTRAF6. The NACHT-LRR domain is the functional domain of rNLRC3. These findings provide insights into the important role of rNLRC3 in combating P. multocida infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Yinan Li, Xingye Lian, Wenwen Yang, Jinyuan Leng, Jiejie Sun, Yu Liu, Siqi Fan, Lingling Wang, Linsheng Song
Summary: The DM9-containing protein CgDM9CP-7 plays an important role in the innate immune response of molluscs. It is constitutively expressed in various tissues and is up-regulated in haemocytes after stimulation by Vibrio splendidus and lipopolysaccharide (LPS). CgDM9CP-7 binds to various pathogens and exhibits antimicrobial activity, while also regulating the expression of CgMyD88 and CgIL-17.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Xiuwen Bao, Haoran Song, Liying He, Yong Li, Shuqi Niu, Jinlin Guo
Summary: This study focuses on the interaction between Hepialus xiaojinensis larvae and Ophiocordyceps sinensis during the infection process. The results show that the infected third instar larvae enhance antioxidant defense ability, while the infected fourth instar larvae reduce this ability and allocate more energy to growth and development.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)