Article
Fisheries
Minxuan Yang, Zhijie Lu, Fenglin Li, Fei Shi, Fanbin Zhan, Lijuan Zhao, Yanan Li, Jun Li, Li Lin, Zhendong Qin
Summary: The study revealed that fish red blood cells are not only responsible for oxygen transportation, but also potentially play a role in immune response against bacterial infections. After incubation with E. coli, grass carp RBCs showed changes in gene expression, activating various immune responses.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Jianhua Feng, Zhao Jia, Gaoliang Yuan, Xiaozhen Zhu, Qin Liu, Kaizheng Wu, Junya Wang, Jun Zou
Summary: This study investigates the expression and antibacterial activities of three grass carp beta-defensin peptides. The results show that these peptides are constitutively expressed in tissues, with the highest expression in the skin. They also demonstrate broad antibacterial activity against fish bacterial pathogens.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Lihan Zhang, Na Xu, Xinlu Liu, Kommaly Onxayvieng, Lu Liu, Rong Tang, Dapeng Li
Summary: The study found that exercise training at low and medium water velocities benefited the growth of grass carp and increased muscle fiber density.
Article
Fisheries
Yuting Qin, Zhaosheng Sun, Wei Wang, Jiawen Xu, Bangjie Wang, Zhao Jia, Xia Li, Junya Wang, Qian Gao, Xinhua Chen, Jun Zou
Summary: CD3 is a crucial component of the TCR-CD3 complex in adaptive immunity, with its homologues CiCD3γ/δ and CiCD3ε identified in grass carp and shown to be highly expressed in healthy fish and modulated by infections. A monoclonal antibody against CiCD3γ/δ was successfully produced, which showed good reactivity in Western blotting and flow cytometry analysis. The CiCD3γ/δ(+) cells were found to be localized in the gills and head kidney, accounting for a small percentage of lymphocytes in different organs.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Shanshan Zeng, Meifeng Li, Xining Cheng, Shina Lu, Zhiqing Feng, Zeyin Jiang, Zhichao Sun, Xiaowen Xu, Huiling Mao, Chengyu Hu
Summary: In this study, it was found that grass carp DYRK2 induces cell apoptosis by increasing the phosphorylation level of p53.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Huiqin Niu, Yifan Pang, Lingli Xie, Qiaozhen Yu, Yubang Shen, Jiale Li, Xiaoyan Xu
Summary: This study investigates the evolutionary background and expression regulation of grass carp miRNAs. Multiple novel miRNAs have emerged during grass carp evolution, and both duplication and de novo production contribute to miRNA cluster formation, with de novo creation being more prevalent. Clustered miRNAs are more conserved and change slower, while unique miRNAs tend to have higher evolution rates and lower expression levels.
Article
Chemistry, Applied
Ran Li, Shuhong Li, Zhiguang Chen, Yu Jin, Shulei Li, Song Li, Zhizi Bai
Summary: The study on grass carp stefin A gene reveals its heat and acid-base tolerance, inhibitory effects on enzymes, and highest expression level in the hepatopancreas. The results provide insights into the physical and biochemical properties of grass carp stefin A.
Article
Immunology
Meifeng Li, Jihuan Hu, Huiling Mao, Dongming Li, Zeyin Jiang, Zhichao Sun, Tingting Yu, Chengyu Hu, Xiaowen Xu
Summary: In this study, it was found that grass carp KAT8 blocked the activities of IRF3 and IRF7 by acetylating them, resulting in the inhibition of nucleic acids-induced innate immune response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Hongyan Yu, Xuewen Jia, Yifan Pang, Huiqin Niu, Biao Du, Xiaoyan Xu, Jiale Li
Summary: In this study, it was found that the TBK1 gene in grass carp could be upregulated by bacterial infection. Overexpression of TBK1 could reduce the number of adhesive bacteria in cells and promote cellular migration, proliferation, vitality, and anti-apoptosis ability. TBK1 could activate the NF-kappa B signaling pathway and induce autophagy in cells. These findings provide important information about the defense and immune mechanisms used by grass carp against pathogens.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Guokun Yang, Xiaomin Liang, Shuangyang Xu, Huimin Cai, Limei Ma, Xulu Chang, Yanmin Zhang, Liping Yang, Xiaolin Meng
Summary: This study focused on the involvement of Igf3 in fish reproduction, particularly in grass carp, by isolating the gene and assessing its functions through various experiments. Igf3 was found to regulate gene expression in multiple tissues, affecting growth-related genes and glucose metabolism. This research is the first to reveal the mechanism by which Igf3 regulates the expression of growth-related genes in teleosts.
Article
Fisheries
Xue-Mei Xiong, Wei-Jie Huang, Qiang Dong, Dong-Yang Zhang, Shi-Ming Wan, Ze-Xia Gao
Summary: This study evaluated the genetic parameters of intermuscular bone (IB) traits in grass carp (Ctenopharyngodon idella), finding low heritability but a strong genetic correlation between body height and IB number.
Article
Fisheries
Kai Hao, Yu Wang, Bin Zhu, Fei Yu, Zhe Zhao, Gao-Xue Wang
Summary: This study demonstrates that surface display of GCRV core antigen vaccine and invasion component from aquatic pathogenic microorganism on nonpathogenic Escherichia coli can effectively prevent GCRV viral diseases via the immersion administration approach.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Hualiang Liang, Hao Xu, Xianping Ge, Jian Zhu, Mingchun Ren, Haifeng Mi
Summary: This study investigated the effects of water temperature and dietary protein levels on the growth and nutritional metabolism of juvenile grass carp. The results showed that appropriate dietary protein levels and higher water temperature improved the growth performance and feed utilization efficiency of grass carp. Grass carp at higher temperature exhibited higher metabolic levels and gene expression levels. The protein requirements of grass carp juveniles varied with water temperature.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Yapeng Liu, Hailing Du, Shanghong Wang, Yangfeng Lv, Hang Deng, Kaile Chang, Pengcheng Zhou, Chengyu Hu
Summary: The study reveals that TNK1 plays a crucial role in the JAK-STAT pathway in fish, enhancing the expression of IFN I.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Wei Wei, Junya Wang, Qingyu Min, Zhao Jia, Kangyong Chen, Hao Feng, Jun Zou
Summary: The study shows that CCL19a2 and CCL19b can promote migration of immune cells and are involved in recruitment of antigen presenting cells in fish. These chemokines exhibit activity and significance both in vitro and in vivo.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Virology
Jie Zhang, Hong Guo, Fuxian Zhang, Qingxiu Chen, Mingxian Chang, Qin Fang
Article
Fisheries
Xiao Man Wu, Lu Cao, Yi Wei Hu, Ming Xian Chang
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Immunology
Xiao Man Wu, Jie Zhang, Peng Wei Li, Yi Wei Hu, Lu Cao, Songying Ouyang, Yong Hong Bi, Pin Nie, Ming Xian Chang
JOURNAL OF IMMUNOLOGY
(2020)
Review
Fisheries
Ming Xian Chang, Fan Xiong, Xiao Man Wu, Yi Wei Hu
Summary: NLR proteins are immune sensors that recognize pathogen patterns, with different species having varying numbers of NLRs. The zebrafish genome contains almost 400 NLR proteins. These proteins can positively or negatively regulate immune responses and inflammatory signaling cascades.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ming Xian Chang, Fan Xiong
Article
Fisheries
Fan Xiong, Lu Cao, Xiao Man Wu, Ming Xian Chang
Summary: This study demonstrates the conserved antiviral role of gpbar1 and its function in regulating glycerophospholipids metabolism in teleost.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Ming Xian Chang
Summary: This paper summarizes recent advances in understanding of negative regulators of the RLR signaling pathway in fish, with a focus on regulatory mechanisms inhibiting the function of RIG-I, MDA5, and other factors. The study also discusses important directions for future research in this field.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Pengwei Li, Mingxian Chang
Summary: Oxidative stress plays a significant role in inflammatory diseases, characterized by the overproduction of reactive oxygen species (ROS). Inflammatory signaling cascades are initiated through the recognition of DAMPs and PAMPs by PRRs, with different PRRs families affecting the production of ROS and/or oxidative stress. Studying the correlation between oxidative stress and PRRs-mediated signaling pathways is crucial for future therapeutic strategies in inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Xiaoman Wu, Fan Xiong, Hong Fang, Jie Zhang, Mingxian Chang
Summary: In this study, the functional correlation between NOD1 and histone H2A variant in response to Streptococcus agalactiae infection was reported. The deficiency of NOD1 promoted S. agalactiae proliferation and decreased larval survival in zebrafish. The results highlight the importance of NOD1 deficiency in immune-related and metabolic pathways regulation, and the correlation between zebrafish NOD1 and histone H2A variant in defense against S. agalactiae infection.
Article
Immunology
Jie Zhang, Xiao Man Wu, Qin Fang, Yong Hong Bi, Pin Nie, Ming Xian Chang
Summary: The study showed that nonstructural proteins NS38 and NS80 of GCRV can evade host antiviral immune response by targeting the RLR signaling pathway, preventing the production of IFN-stimulated genes and facilitating GCRV replication.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Biology
Lu Cao, Hong Fang, Dong Yan, Xiao Man Wu, Jie Zhang, Ming Xian Chang
Summary: The adhesion molecule CD44 drives host defense in zebrafish by regulating apoptosis and autophagy. CD44a variants mediate antibacterial responses through different mechanisms, with CD44a_tv1 promoting apoptosis and CD44a_tv2 inhibiting autophagy to fight against E. piscicida infection.
COMMUNICATIONS BIOLOGY
(2022)
Article
Immunology
Jie Zhang, Ming Xian Chang
Summary: This study reveals that a grass carp TBK1 isoform named gcTBK1_tv3 plays a role in restraining the replication and infection of grass carp reovirus (GCRV) by promoting the degradation of the nonstructural proteins NS80 and NS38, impairing the formation of viral inclusion bodies.
JOURNAL OF IMMUNOLOGY
(2023)
Article
Fisheries
Si Yao Zheng, Xinbin Shao, Zhitao Qi, Maocang Yan, Min Hui Tao, Xiao Man Wu, Lining Zhang, Jianzhong Ma, An Li, Ming Xian Chang
Summary: This study firstly demonstrates the functional characterization of zebrafish nos2a during bacterial infection, revealing its regulation of ROS and NO as well as the expression of proinflammatory cytokines, antibacterial genes, and oxidant factors. Furthermore, it finds that inhibition of NO can be effective in protecting against E. piscicida infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Review
Fisheries
Peng Wei Li, Jie Zhang, Ming Xian Chang
Summary: AqRVs are a type of virus that can infect various wild or cultured bony fishes, causing serious muscle and viscera haemorrhage syndrome and significant economic losses worldwide. Research on the viral proteins of AqRVs has revealed their roles in RNA transcription, virion assembly, cell attachment, and viral replication. Additionally, the structural and functional studies of AqRVs have provided insights into their replication and immune evasion strategies, leading to the development of vaccines and antiviral agents for GCRV, the most pathogenic AqRV.
REVIEWS IN AQUACULTURE
(2023)
Article
Microbiology
Yun Jie Song, Jie Zhang, Jun Xiao, Hao Feng, Zhen Xu, Pin Nie, Ming Xian Chang
Summary: This study reveals that in the absence of vitamin D, grass carp reovirus (GCRV) utilizes host vitamin D receptors Vdra/Vdrb to increase hmgcr-dependent cholesterol synthesis for promoting the production of viral inclusion bodies (VIBs). The negative regulation of Vdrs during viral infection can be prevented by vitamin D treatment, enhancing resistance against GCRV.
MICROBIOLOGY SPECTRUM
(2023)
Article
Fisheries
Ratiporn Thongsoi, Siriluk Maskaew, Panumas Puechpon, Benjaporn Noppradit, Napassawan Inaek, Prapaporn Utarabhand, Phanthipha Runsaeng
Summary: In this study, a PPAE gene called FmPPAE1 was cloned and analyzed in banana shrimp. FmPPAE1 plays a vital role in regulating the proPO system, exhibits antibacterial properties, and contributes to the suppression of bacterial proliferation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jishuang Tan, Paizelaiti Sahaer, Huan Li, Wei Han, Hongyan Sun
Summary: Circular RNAs (circRNA) are involved in gene expression and affect cellular processes such as transcription, splicing, and translation by interacting with microRNA and RNA binding proteins. A study identified circDNAJB6 as a circular RNA abundant in chicken macrophages after bacterial infection, and found that circDNAJB6 exacerbates cellular injury and interacts with multiple miRNAs and target genes, potentially impacting autophagy and MAPK signaling pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Rodrigo Guabiraba, Damaris Ribeiro Rodrigues, Paul T. Manna, Melanie Chollot, Vincent Saint-Martin, Sascha Trapp, Marisa Oliveira, Clare E. Bryant, Brian J. Ferguson
Summary: The innate immune response relies on the ability of host cells to detect and respond to microbial nucleic acids. Toll-like receptors (TLRs) play a crucial role in this process by distinguishing self from non-self. This study focused on TLR21, an avian TLR that recognizes bacterial DNA motifs. The findings suggest that avian TLR21 shares similar activation mechanisms to mammalian TLR9, highlighting the conservation of nucleic acid sensing mechanisms across species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Safieh Zeinali, Kate Sutton, Lonneke Vervelde
Summary: Chickens have a unique immune structure characterized by the absence of draining lymph nodes and the presence of well-developed mucosa-associated lymphoid tissue. The structure and development of chicken lymphoid tissues in the intestine, particularly Peyer's patches, have been poorly studied. However, the use of CSF1R-eGFP reporter transgenic chickens has allowed for the visualization and investigation of the development and distribution of these tissues. This research provides insights that could benefit mucosal vaccination strategies and the development of targeted vaccine delivery systems.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Retraction
Fisheries
G. -g. Li, Z. -z. Guo, X. -f. Ma, N. Cao, S. -n. Geng, Y. -q. Zheng, M. -j. Meng, H. -h. Lin, G. Han, G. -j. Du
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jinyue Yang, Peng Zhou, Wanrong Wu, Qingxiang Zhang, Dong Chen, Rui Luo
Summary: This study cloned and characterized the full-length sequence of duck MRE11 (duMRE11) for the first time, and found that this protein plays an important role in the innate immune response of ducks.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Mengjiao Guo, Jiaqi Zhang, Mingtao Li, Xiaorong Zhang, Yantao Wu
Summary: rNLRC3 negatively regulates the NF -KB signaling pathway induced by P. multocida in rabbits by inhibiting the activation of NF -KB, reducing the expression of inflammatory cytokines, and interacting with rTRAF4 and rTRAF6. The NACHT-LRR domain is the functional domain of rNLRC3. These findings provide insights into the important role of rNLRC3 in combating P. multocida infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Yinan Li, Xingye Lian, Wenwen Yang, Jinyuan Leng, Jiejie Sun, Yu Liu, Siqi Fan, Lingling Wang, Linsheng Song
Summary: The DM9-containing protein CgDM9CP-7 plays an important role in the innate immune response of molluscs. It is constitutively expressed in various tissues and is up-regulated in haemocytes after stimulation by Vibrio splendidus and lipopolysaccharide (LPS). CgDM9CP-7 binds to various pathogens and exhibits antimicrobial activity, while also regulating the expression of CgMyD88 and CgIL-17.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Xiuwen Bao, Haoran Song, Liying He, Yong Li, Shuqi Niu, Jinlin Guo
Summary: This study focuses on the interaction between Hepialus xiaojinensis larvae and Ophiocordyceps sinensis during the infection process. The results show that the infected third instar larvae enhance antioxidant defense ability, while the infected fourth instar larvae reduce this ability and allocate more energy to growth and development.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)