期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 38, 期 2, 页码 275-284出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2012.06.012
关键词
Gloverin; Toll-Spatzle pathway; Lipopolysaccharide; Lipoteichoic acid; Peptidoglycan; Antimicrobial activity
资金
- NIGMS NIH HHS [R01 GM066356] Funding Source: Medline
Hyalophora gloveri gloverin is a glycine-rich and heat stable antimicrobial protein with activity mainly against Escherichia coli. However. Spodoptera exigua gloverin is active against a Gram-positive bacterium but inactive against E. coli. In this study, we investigated expression profile, binding ability and antimicrobial activity of Manduca sexta gloverin (MsGlv). Msglv transcript was detected in several tissues of naive larvae with higher levels in the midgut and testis. Expression of Msglv mRNA in larvae was up-regulated by active Spatzle-C108 and peptidoglycans (PGs) of E. coli and Staphylococcus aureus, and the activation was blocked by pre-injection of antibody to M. sexta Toll, suggesting that Msglv expression is regulated by the Toll-Spatzle pathway. Recombinant MsGlv bound to the O-specific antigen and outer core carbohydrate of lipopolysaccharide (LPS). Gram-positive lipoteichoic acid (LTA) and PG, and laminarin, but not to E. coli PG or mannan. MsGlv was active against Bacillus cereus, Saccharomyces cerevisiae and Cryptococcus neoformans, but was almost inactive against E. coli and S. aureus. Our results suggest that gloverins are active against some bacteria and fungi. (C) 2012 Elsevier Ltd. All rights reserved.
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