期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 35, 期 8, 页码 835-839出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2011.03.001
关键词
Peptidoglycan recognition protein; Innate immunity; D-Alanylation; Wall teichoic acid; Staphylococcus aureus; Insect
资金
- Pusan National University
We recently reported that D-alanylation of Staphylococcus aureus wall teichoic acid (WTA) mitigates an induction of the Toll-mediated humoral response in Drosophila by interfering with peptidoglycan (PG) recognition by PG recognition protein-SA (PGRP-SA). Here, we investigated the mode of this interference by using an in vitro cell free system from larvae of the coleoptran insect Tenebrio molitor. WTA modification on PG had a potent inhibitory effect on PGRP-SA-mediated Toll proteolytic cascade activation, and the D-alanylation of WTA enhanced its inhibitory effect. Purified D-alanylated WTA released from PG lost its inhibitory action on both Toll cascade activation and PGRP-SA binding to insoluble PG. The inhibition of PGRP-SA binding to PG by D-alanylated WTA took place not only on polymeric PG but also on WTA-attached disaccharide units of monomeric PG. These results suggest that D-alanylation-mediated evasion requires the covalent bonding of D-alanylated WTA to PG, but not net-like cross-linking structure of PG. (C) 2011 Elsevier Ltd. All rights reserved.
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