期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 34, 期 11, 页码 1160-1169出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2010.06.010
关键词
Caenorhabditis elegans; Development; Fecundity; Immune system; MMP; Model organism; Molting
资金
- German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) [AL902/4-1]
The genome of Caenorhabditis elegans includes six homologs of matrix metalloproteinases (MMPs). The C. elegans MMP gene zmp-1 has recently been shown to be involved in anchor cell invasion during post-embryonic vulva! development. Here, we identified H19M22.3 (zmp-2) as a pleiotropic MMP gene regulating disease resistance, molting, larval development, and fecundity. Zmp-2(RNAi) nematodes showed significant lifespan reduction during infection with pathogenic Photorhabdus luminescence. Moreover, we observed molting defects indicating a direct or regulative role in extracellular matrix degradation during ecdysis, delayed larval to adult development, and reduced offspring production in hermaphrodite adults. GFP-expressing nematodes revealed predominant expression of zrnp-2 in multiple cells during embryogenesis; in hypodermal, muscle, and somatic gonad cells during larval development: and in developing and mature spermathecae in the L4 larval stage and adults. These results give evidence for pleiotropic roles of zmp-2 and provide novel insights into evolutionarily conserved and derived MMP functions in C. elegans. (C) 2010 Elsevier Ltd. All rights reserved.
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