Article
Immunology
Madison A. Emery, Bradford A. Dimos, Laura D. Mydlarz
Summary: By investigating the PRR repertoires of cnidarians, researchers found that anthozoans possess more typical PRRs compared to medusozoans. Anthozoans also have a higher number of PRRs, particularly in NOD-like receptors and C-type lectins. Additionally, symbiotic, sessile, and colonial cnidarians have expanded PRR repertoires compared to their non-symbiotic, mobile, and solitary counterparts.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Gaelle Botton-Amiot, Pedro Martinez, Simon G. Sprecher
Summary: Sea anemones without centralized nervous systems can still form associative memories, as shown by their conditioned response to light and electric shock. These findings shed light on cnidarian behavior and raise fundamental questions about the origin and evolution of cognition in brainless animals.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Entomology
Zhihao Ming, Zhiqiang Chen, Hao Tong, Xia Zhou, Tingting Feng, Dai Jianfeng
Summary: C-type lectins (CTLs) are a family of proteins that play important roles in innate immune defenses against microorganisms. They bind to a broad range of ligands and mediate immune responses to clear pathogens. Recent studies have shown that CTLs are essential for arthropods' immune responses to arthropod-borne pathogens. This article reviews the current understanding of CTLs derived from important medical arthropods.
Review
Entomology
Pengyu Chen, Kristof De Schutter, Els J. M. Van Damme, Guy Smagghe
Summary: Lectins are carbohydrate-binding proteins that recognize and selectively bind specific sugar structures. They are found in plants, animals, and microorganisms, playing important roles in immune systems. While insect lectins are not well studied, plant lectins have been characterized and known to possess immunomodulatory properties in vertebrates. The study of lectins and their functions in insects lags behind, but understanding their role in insect immunity is crucial for the development of pest control strategies.
Article
Fisheries
Hongtao Liu, Shengtao Guo, Rong Wang, Yugui He, Qiong Shi, Zhaobin Song, Mingqiu Yang
Summary: The study used proteomic sequencing of hepatopancreas in Vibrio harveyi-infected whiteleg shrimp and found a significant number of DEPs, specifically showing changes in the expression levels of C-type lectins after infection. Different members of the CTL family exhibit varied responses to Vibrio harveyi infection, suggesting potential differential functions and providing theoretical guidance for further research on immune responses and pathogen infections in shrimps.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Angela Holder, Jeannine Kolakowski, Chloe Rosentreter, Ellen Knuepfer, Sabine A. F. Jegouzo, Oliver Rosenwasser, Heather Harris, Lotta Baumgaertel, Amanda Gibson, Dirk Werling
Summary: Innate immune receptors form complexes with secondary receptors to activate multiple signaling pathways, regulating homeostasis and immunity. A bovine homologue of Mincle (boMincle) has been characterized to be structurally and functionally similar to the human orthologue (huMincle), although there are some differences. BoMincle is predominantly expressed by myeloid cells, but mRNA transcripts were also detected in granulocytes, B cells, and T cells. BoMincle has the potential to recognize endogenous ligands, suggesting its role in homeostasis in cattle.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Yi Zhang, Mengqi Ni, Peng Zhang, Yunhui Bai, Bin Zhou, Jinbin Zheng, Zhaoxia Cui
Summary: This study identified novel immune-related genes in Portunus trituberculatus, showcasing their diverse transcriptional profiles across different tissues and rapid response to bacterial pathogens. The findings provide insights into the immune response of P. trituberculatus and lay theoretical foundations for precise disease prevention and control strategies in aquaculture. The specific anti-bacterial activities exhibited by certain genes suggest potential applications in disease control.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Ming Luo, Sanghyok Ri, Longlong Liu, Songnam Ri, Yongchol Kim, Tongchol Kim, Kwangjin Ju, Weishang Zhou, Difei Tong, Wei Shi, Guangxu Liu
Summary: In this study, a novel CLEC3B gene was identified in the golden pompano (Trachinotus ovatus) using transcriptomic and BLAST screening. The characterization and expression patterns of the CLEC3B gene were analyzed, and it was found to be a Ca2+-dependent galactose-binding lectin with a single copy of carbohydrate recognition domain. The study also demonstrated that this gene is constitutive and inducible in the innate immune response of the golden pompano.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Jingwen Liu, Xiaocong Lou, Yuying Yang, Xiaoyuan Zheng, Yunkai Zhang, Peng Zhou, Shun Yang, Mengmeng Huang, Hui Fei
Summary: C-type lectins are pattern recognition receptors involved in innate immunity. Two C-type lectins were identified from the mud crab Scylla paramamosain, and they were found to have broad nonself-recognition spectrum and play a key role in pathogen elimination by binding to various pathogen-associated molecular patterns and enhancing phagocytosis and encapsulation activities of hemocytes.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Ting Kang, Yanting Xia, Taiwei Dong, Xiaoyuan Zheng, Shun Yang, Shichao Qian, Mengmeng Huang, Hui Fei
Summary: A new C-type lectin, PtCTL-9, was identified from swimming crab and showed broad non-self immunity, acting as an opsonin to promote phagocytosis and hemocyte encapsulation. The QPN motif was found to be important for PtCTL-9's immune function.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Magda Lewandowska, Ton Sharoni, Yael Admoni, Reuven Aharoni, Yehu Moran
Summary: The study characterized the antiviral immune response of the cnidarian Nematostella vectensis and showed that its RLR paralogs exhibit features distinctive for both vertebrate and invertebrate systems. The functional evidence provided supports the conserved role of RLRs in initiating immune response to dsRNA before the cnidarian-bilaterian split, laying a foundation for further research on the evolution of immune responses to RNA viruses.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Fisheries
Hong -Ye Chen, Wen-Ya Li, Jie Wang, Gong-Wen Bo, Gui-Wen Yang, Hui -Ting Yang
Summary: The study investigates the antimicrobial mechanism of a C-type lectin (CTL) containing two carbohydrate recognition domains (DClec). It is found that DClec regulates the JNK signaling pathway and adjusts antimicrobial peptide (AMP) expression to eliminate pathogens. It is also observed that DClec is secreted into the hemolymph and binds to bacteria, promoting their elimination. The findings suggest that DClec plays an important role in the immune response of crustaceans.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tomoyuki Shimazu, Mai Suzuki, Ryo Takasaki, Asuka Besshi, Yuna Suzuki, Yoichiro Iwakura
Summary: This study investigated the relationship between lactic acid bacteria (LAB) and innate immune receptors. The researchers found that C-type lectin receptors (CLRs) are involved in the immune-stimulating function of LAB. The interaction between LAB and CLRs can reduce the levels of certain cytokines and is only observed in heat-killed LAB. These findings contribute to a better understanding of the immunomodulatory effects of LAB.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Entomology
Dongxu Shen, Meijin Tong, Jiyun Guo, Xianghan Mei, Dingguo Xia, Zhiyong Qiu, Qiaoling Zhao
Summary: Insect innate immunity is initiated by pattern recognition receptors (PRRs) such as C-type lectins (CTLs), which play various roles including pathogen recognition, prophenoloxidase stimulation, and cellular immunity regulation. The study cloned a CTL gene named CTL-S6 from the silkworm, Bombyx mori, and found it to be involved in recognizing foreign pathogens, stimulating the prophenoloxidase pathway, and participating in innate immunity.
JOURNAL OF INSECT SCIENCE
(2021)
Article
Fisheries
Tongchol Kim, Sanghyok Ri, Kwangjin Ju, Wei Shi, Weishang Zhou, Yihan Yu, Sangryong Ri, Hyoksong Ri, Songsu Yun, Junjin Ri, Guangxu Liu
Summary: In this study, a new C-type lectin (CTL) named TgCTL-1 was identified in the blood clam. TgCTL-1 was highly expressed in hemocytes and visceral mass under normal condition and significantly up-regulated upon pathogen challenge. The recombinant TgCTL-1 displayed agglutinating and binding activities against both gram-positive and gram-negative bacteria and enhanced the phagocytic activity of hemocytes. These results suggest that TgCTL-1 plays important roles in the immune defense of blood clam.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jason S. Presnell, Elizabeth Wirsching, Virginia M. Weis
Summary: This study investigates the development and morphological characteristics of pedal laceration in the tropical sea anemone Aiptasia. The results show that aposymbiotic (without algae) lacerates develop tentacles earlier than symbiotic lacerates, while symbiotic lacerates end up with a greater number of tentacles after 20 days. The study also reveals the differential effects of symbiotic and nutritional state on pedal lacerate development. These findings provide important insights for further understanding cnidarian-dinoflagellate symbiosis.
Article
Cell Biology
Lucy M. Gorman, Migle K. Konciute, Guoxin Cui, Clinton A. Oakley, Arthur R. Grossman, Virginia M. Weis, Manuel Aranda, Simon K. Davy
Summary: In the symbiotic relationship between cnidarians and dinoflagellates, the presence of symbionts affects the host's cell cycle, cell division, and apoptosis, contributing to the persistence of the symbionts within the host. The symbionts stimulate host cell division in the epidermis, while inhibiting apoptosis in the gastrodermis. These findings provide insights into the mechanisms of cnidarian cell proliferation and symbiont maintenance.
CELLULAR MICROBIOLOGY
(2022)
Article
Microbiology
Shumpei Maruyama, Paige E. Mandelare-Ruiz, Mark McCauley, Wenjing Peng, Byeong Gwan Cho, Junyao Wang, Yehia Mechref, Sandra Loesgen, Virginia M. Weis
Summary: This study investigates the effects of heat stress on the symbiotic relationship between corals and dinoflagellate algae. Using the sea anemone Aiptasia and its native symbiont Breviolum minutum as a model system, the researchers found that heat stress reduces algal colonization and alters the cell surface molecules of the algae. This study contributes to our understanding of coral-algal symbiosis and its breakdown under heat stress.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biology
Maggie Wuerz, Caitlin A. Lawson, Maiken Ueland, Clinton A. Oakley, Arthur R. Grossman, Virginia M. Weis, David J. Suggett, Simon K. Davy
Summary: The establishment and maintenance of symbiosis between a cnidarian host and its dinoflagellate symbionts is crucial for the success of coral reefs. This study explored the production of biogenic volatile organic compounds (BVOCs) in this symbiosis using gas chromatography-mass spectrometry. The results revealed 152 BVOCs, with differential abundances between symbiotic and aposymbiotic anemones, as well as between symbiotic anemones and cultured dinoflagellates. The findings suggest that symbiosis may have a greater impact on symbiont physiology than host physiology.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Review
Microbiology
Giulia Puntin, Michael Sweet, Sebastian Fraune, Monica Medina, Koty Sharp, Virginia M. Weis, Maren Ziegler
Summary: Stony corals form the foundation of coral reefs, which are significant ecosystems in both ecological and economic aspects. However, climate change and other human-induced stressors pose threats to the existence of these ecosystems, leading to coral bleaching, disease, and ultimately coral mortality. Despite extensive research, the underlying mechanisms and potential solutions to these destructive phenomena are still not fully understood.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Sheila A. Kitchen, Duo Jiang, Saki Harii, Noriyuki Satoh, Virginia M. Weis, Chuya Shinzato
Summary: The study examines the effects of thermal stress and symbiosis onset on colonization dynamics, survival, metamorphosis, and gene expression in coral larvae. It finds that thermal stress decreases symbiont colonization and density, and influences larval survival and metamorphosis. The transcriptomic responses to colonization and thermal stress are mostly independent. Furthermore, host immune suppression turns to immune activation under heat stress. The study suggests that colonization may hinder larval survival and recruitment under projected climate scenarios.
Editorial Material
Astronomy & Astrophysics
Jasmin Graham, Angelos Hannides, Nabir Mamnun, Lina E. Sitz, Ian D. Walsh, Elisha M. Wood-Charlson, Leandro Ponsoni
Summary: This article presents three independent commentaries on the application and challenges of the Integrated, Coordinated, Open, and Networked (ICON) principles and the Findable, Accessible, Interoperable, and Reusable (FAIR) principles in ocean sciences. Progress has been made in areas such as field sampling, global collaboration and data sharing, and increasing diversity, but there are still opportunities for improvement.
EARTH AND SPACE SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Aidan L. Huene, Jack C. Koch, Lucia Arregui, Yue Liu, Matthew L. Nicotra, Virginia M. Weis, Terrence R. Tiersch
Summary: This study established a cryopreservation approach for Hydractinia symbiolongicarpus sperm, using open hardware and 3D printing technology. The results showed that, under specific conditions, a certain fertilization rate could be achieved. Establishing a repository for Hydractinia resources is crucial for future research and development.
Article
Biochemical Research Methods
Dylan Chivian, Sean P. Jungbluth, Paramvir S. Dehal, Elisha M. Wood-Charlson, Richard S. Canon, Benjamin H. Allen, Mikayla M. Clark, Tianhao Gu, Miriam L. Land, Gavin A. Price, William J. Riehl, Michael W. Sneddon, Roman Sutormin, Qizhi Zhang, Robert W. Cottingham, Chris S. Henry, Adam P. Arkin
Summary: Uncultivated Bacteria and Archaea, which make up the majority of Earth's species, can now have their genomes extracted using shotgun sequencing. The U.S. Department of Energy Systems Biology Knowledgebase (KBase) platform offers a series of analysis steps and data products to facilitate the recovery of high-quality metagenome-assembled genomes (MAGs) from microbiomes. This platform allows users to go from sequencing reads to MAGs, which can be further analyzed with other KBase capabilities.
Correction
Biochemical Research Methods
Dylan Chivian, Sean P. Jungbluth, Paramvir S. Dehal, Elisha M. Wood-Charlson, Richard S. Canon, Benjamin H. Allen, Mikayla M. Clark, Tianhao Gu, Miriam L. Land, Gavin A. Price, William J. Riehl, Michael W. Sneddon, Roman Sutormin, Qizhi Zhang, Robert W. Cottingham, Chris S. Henry, Adam P. Arkin
Article
Microbiology
Immy A. Ashley, Sheila A. Kitchen, Lucy M. Gorman, Arthur R. Grossman, Clinton A. Oakley, David J. Suggett, Virginia M. Weis, Sabrina L. Rosset, Simon K. Davy
Summary: This study analyzed the phosphatidylinositol (PI) signaling pathway in the cnidarian-dinoflagellate symbiosis and found that it is conserved in symbiotic cnidarians and expanded in symbiotic dinoflagellates. The study also identified unique proteins in the symbiotic dinoflagellates that are not found in non-symbiotic unicellular organisms.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Amirhossein Gheitanchi Mashini, Clinton A. Oakley, Sandeep S. Beepat, Lifeng Peng, Arthur R. Grossman, Virginia M. Weis, Simon K. Davy
Summary: We used liquid chromatography-mass spectrometry to study the proteomic changes in the symbiotic relationship between Breviolum minutum and the sea anemone Exaiptasia diaphana. Both the symbiotic and nutritional states had significant impacts on the B. minutum proteome. These results provide insights into the physiological changes of the symbiont in response to the host microenvironment and nutrient availability.
Editorial Material
Biochemistry & Molecular Biology
Elisha M. Wood-Charlson
Summary: Systems biology research requires collaborative efforts to collect and share data for integration, but effective data sharing is challenging and requires alignment and coordination among partners, organizations, repositories, and journals. As a community of systems biology researchers, we need to improve the efficiency of data sharing and ensure that shared data receives the recognition and citations it deserves.
Article
Multidisciplinary Sciences
Guoxin Cui, Migle K. Konciute, Lorraine Ling, Luke Esau, Jean -Baptiste Raina, Baoda Han, Octavio R. Salazar, Jason S. Presnell, Nils Raedecker, Huawen Zhong, Jessica Menzies, Phillip A. Cleves, Yi Jin Liew, Cory J. Krediet, Val Sawiccy, Maha J. Cziesielski, Paul Guagliardo, Jeremy Bougoure, Mathieu Pernice, Heribert Hirt, Christian R. Voolstra, Virginia M. Weis, John R. Pringle, Manuel Aranda
Summary: Symbiotic cnidarians, such as corals and anemones, have evolved efficient molecular mechanisms for nutrient distribution and recycling in order to thrive in nutrient-poor ocean environments. Using the sea anemone Aiptasia, researchers have discovered that glucose and the presence of algae induce the up-regulation and relocalization of glucose and ammonium transporters, which are critical for symbiont functioning and organism-wide nitrogen assimilation. These findings provide insight into the molecular mechanisms underlying nitrogen conservation and recycling in symbiotic cnidarians.
Article
Biology
Maggie Wuerz, Caitlin A. Lawson, Clinton A. Oakley, Malcolm Possell, Shaun P. Wilkinson, Arthur R. Grossman, Virginia M. Weis, David J. Suggett, Simon K. Davy
Summary: The symbiosis between corals and their dinoflagellate symbionts plays a crucial role in supporting coral metabolism and survival. This study investigates how the identity of the symbiont affects the release of BVOCs and the changes in the host microbiome. The findings contribute to our understanding of coral response to climate change and provide a platform for future studies on the metabolic and signaling roles of BVOCs in this symbiosis.
Article
Fisheries
Ratiporn Thongsoi, Siriluk Maskaew, Panumas Puechpon, Benjaporn Noppradit, Napassawan Inaek, Prapaporn Utarabhand, Phanthipha Runsaeng
Summary: In this study, a PPAE gene called FmPPAE1 was cloned and analyzed in banana shrimp. FmPPAE1 plays a vital role in regulating the proPO system, exhibits antibacterial properties, and contributes to the suppression of bacterial proliferation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jishuang Tan, Paizelaiti Sahaer, Huan Li, Wei Han, Hongyan Sun
Summary: Circular RNAs (circRNA) are involved in gene expression and affect cellular processes such as transcription, splicing, and translation by interacting with microRNA and RNA binding proteins. A study identified circDNAJB6 as a circular RNA abundant in chicken macrophages after bacterial infection, and found that circDNAJB6 exacerbates cellular injury and interacts with multiple miRNAs and target genes, potentially impacting autophagy and MAPK signaling pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Rodrigo Guabiraba, Damaris Ribeiro Rodrigues, Paul T. Manna, Melanie Chollot, Vincent Saint-Martin, Sascha Trapp, Marisa Oliveira, Clare E. Bryant, Brian J. Ferguson
Summary: The innate immune response relies on the ability of host cells to detect and respond to microbial nucleic acids. Toll-like receptors (TLRs) play a crucial role in this process by distinguishing self from non-self. This study focused on TLR21, an avian TLR that recognizes bacterial DNA motifs. The findings suggest that avian TLR21 shares similar activation mechanisms to mammalian TLR9, highlighting the conservation of nucleic acid sensing mechanisms across species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Safieh Zeinali, Kate Sutton, Lonneke Vervelde
Summary: Chickens have a unique immune structure characterized by the absence of draining lymph nodes and the presence of well-developed mucosa-associated lymphoid tissue. The structure and development of chicken lymphoid tissues in the intestine, particularly Peyer's patches, have been poorly studied. However, the use of CSF1R-eGFP reporter transgenic chickens has allowed for the visualization and investigation of the development and distribution of these tissues. This research provides insights that could benefit mucosal vaccination strategies and the development of targeted vaccine delivery systems.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Retraction
Fisheries
G. -g. Li, Z. -z. Guo, X. -f. Ma, N. Cao, S. -n. Geng, Y. -q. Zheng, M. -j. Meng, H. -h. Lin, G. Han, G. -j. Du
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jinyue Yang, Peng Zhou, Wanrong Wu, Qingxiang Zhang, Dong Chen, Rui Luo
Summary: This study cloned and characterized the full-length sequence of duck MRE11 (duMRE11) for the first time, and found that this protein plays an important role in the innate immune response of ducks.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Mengjiao Guo, Jiaqi Zhang, Mingtao Li, Xiaorong Zhang, Yantao Wu
Summary: rNLRC3 negatively regulates the NF -KB signaling pathway induced by P. multocida in rabbits by inhibiting the activation of NF -KB, reducing the expression of inflammatory cytokines, and interacting with rTRAF4 and rTRAF6. The NACHT-LRR domain is the functional domain of rNLRC3. These findings provide insights into the important role of rNLRC3 in combating P. multocida infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Yinan Li, Xingye Lian, Wenwen Yang, Jinyuan Leng, Jiejie Sun, Yu Liu, Siqi Fan, Lingling Wang, Linsheng Song
Summary: The DM9-containing protein CgDM9CP-7 plays an important role in the innate immune response of molluscs. It is constitutively expressed in various tissues and is up-regulated in haemocytes after stimulation by Vibrio splendidus and lipopolysaccharide (LPS). CgDM9CP-7 binds to various pathogens and exhibits antimicrobial activity, while also regulating the expression of CgMyD88 and CgIL-17.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Xiuwen Bao, Haoran Song, Liying He, Yong Li, Shuqi Niu, Jinlin Guo
Summary: This study focuses on the interaction between Hepialus xiaojinensis larvae and Ophiocordyceps sinensis during the infection process. The results show that the infected third instar larvae enhance antioxidant defense ability, while the infected fourth instar larvae reduce this ability and allocate more energy to growth and development.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
