期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 33, 期 4, 页码 592-600出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2008.10.008
关键词
Crassostrea virginica; Oyster gonadal tissue; Oyster digestive gland; Vibrio spp.; Bacteriostasis; Quantitative real-time PCR
资金
- National Oceans and Atmospheric Association Center of Excellence in Oceans and Human Health at the Hollings Marine Laboratory
In bivalve mollusks the roles of individual tissues in antimicrobial defense remain unclear. In this study, Crassostrea virginica were injected in the adductor muscle with 10(5) live Vibrio campbellii. Major tissues were dissected at 10, 30, 60 or 120 min postinjection (PI): in each tissue undegraded (intact) bacteria were quantified by real-time PCR and culturable bacteria were enumerated by selective plating. At 10 min PI, accumulation of bacteria varied among tissues from approximately 2.4 x 10(3) (labial palps, digestive gland) to 24.2 x 10(3) (gonads) intact Vibrio g(-1). Neither distribution nor accumulation of intact bacteria changed with time except in the hemolymph. In most tissues, more than 80% of intact bacteria were culturable at 10 min PI and culturability decreased with time. In contrast, only 19% of intact bacteria in gonadal tissue could be cultured at 10 min PI, pointing to a major role for the gonadal tissues in antibacterial defense of molluscs. (C) 2008 Elsevier Ltd. All rights reserved.
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