Article
Biochemistry & Molecular Biology
Yu-Jian Wang, Li Sun
Summary: This study investigated the function of a Type Ib crustin from a deep-sea crustacean and revealed its antimicrobial and immunomodulatory functions. The crustin was found to bind and kill bacteria, while reducing the inflammatory response caused by bacteria. The whey acidic protein domain and the C-terminal region of the crustin played different roles in its antimicrobial activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Linxin Dai, Peixun Chen, Danqing Hou, Yue Wang, Yinhuan Zhou, Chengbo Sun
Summary: This study isolated and identified a strain of Vibrio fortis from a biofloc pool, and investigated its pathogenicity and antibiotic sensitivity in Penaeus monodon. The study also explored the immune response and molecular changes in P. monodon infected with V. fortis. The findings provide insights into the study and control of shrimp vibriosis.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Marcel Martinez-Porchas, Jorge Hernandez-Lopez, Francisco Vargas-Albores
Summary: This study aimed to verify the loss of valuable descriptive information in crustin identification during automatic annotation processes of high-throughput sequencing and detect possible crustin sequences reported in Penaeids to establish structural similarities and phylogenetic relationships. The results showed that most sequences meeting the structural criteria obtained from Sanger or transcriptomics were correctly identified as crustin, while those obtained from whole genome sequencing projects were incorrectly classified or uncharacterized, being buried in the generated information. In addition, sequences that met the crustin criteria tended to be grouped into species separated by geographical regions, indicating regional variations in crustin composition.
Article
Fisheries
Wen-Ya Wei, Jian-Hua Huang, Qi-Bin Yang, Fa-Lin Zhou, Song Jiang, Yun-Dong Li, Shi-Gui Jiang, Li-Shi Yang
Summary: As a highly conserved transcription factor family, DMRTs play important roles in sex determination and sexual differentiation in various animals. In this study, a DM-domain gene named PmDMRT11E was identified in Penaeus monodon, and it was found to be highly expressed in the testis and play a crucial regulatory role during embryonic development. Additionally, PmDMRT11E was found to be closely related to gonadal development and spermatogenesis.
AQUACULTURE REPORTS
(2022)
Article
Chemistry, Medicinal
Xinjia Lv, Shihao Li, Yang Yu, Xiaojun Zhang, Fuhua Li
Summary: This study reveals that a crustin called LvCrustin I-2 can resist bacterial infection by regulating the balance of intestinal microbiota and has an impact on the intestinal epithelial barrier and immune function in shrimp.
Article
Fisheries
Prawit Oangkhana, Piti Amparyup, Anchalee Tassanakajon, Elumalai Preetham, Ratree Wongpanya
Summary: In this study, a fibrinogen-related protein in the black tiger shrimp Penaeus monodon was identified and characterized. The PmFreP was found to be mainly expressed in the intestine, able to bind bacteria and facilitate the clearance of pathogens in shrimp innate immunity. These results suggest that PmFreP may serve as pattern recognition receptors implicated in shrimp innate immunity.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Shen Yang, Jian Li, Jude Juventus Aweya, Shaogui He, Shanggui Deng, Wuyin Weng, Yueling Zhang, Guang-Ming Liu
Summary: The histone H4-derived peptide PvH4a exhibits strong antimicrobial activity, especially against Gram-negative bacteria. PvH4a increases the inner membrane permeability of V. parahaemolyticus and has no adverse effects on normal human cells.
Article
Immunology
Manyu Jiang, Roushi Chen, Fangyi Chen, Xuewu Zhu, Ke-Jian Wang
Summary: This study identified a new antimicrobial peptide gene, SpCrus8, in mud crabs and revealed its important role in opsonization and regulation of the immune response. The gene reduced bacterial load and improved survival rate in infected crabs. It exhibited broad distribution in various tissues and had strong binding activity to bacteria and microbial surface components.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chao Peng, Chao Zhao, Pengfei Wang, Lulu Yan, Sigang Fan, Lihua Qiu
Summary: TRIM9 plays a crucial role in regulating innate immune signaling. The identification of a novel TRIM9 homolog (PmTRIM9) in Penaeus monodon revealed its involvement in facilitating V. parahaemolyticus infection by negatively regulating the PmRelish-mediated expression of antimicrobial peptides.
MOLECULAR IMMUNOLOGY
(2021)
Article
Fisheries
Xiaoling Dai, Ximei Nie, Zhengxiao Han, Qian Ren, Xin Huang
Summary: This study identified two glycine-rich crustin genes, Mn-GlyCru3 and Mn-Gly-Cru4, in Macrobrachium nipponense, which play crucial roles in the defense against foreign pathogens. The expression of these genes was significantly altered in different tissues after challenge with bacteria or viruses. The findings suggest that Mn-GlyCru3 and Mn-Gly-Cru4 are important players in the innate immune response against bacterial or viral infections.
Article
Food Science & Technology
Shen Yang, Jingyi Dai, Jude Juventus Aweya, Rong Lin, Wuyin Weng, Yuanhong Xie, Ritian Jin
Summary: This study aimed to improve bacterial inhibition in Pickering emulsions during storage using antimicrobial peptides. A peptide (ARHQGVMVGMGQK), designated SA6, isolated from the broth of salt-fermented shrimp (Penaeus vannamei). Peptide SA6 could stabilize Pickering emulsion while exerting antibacterial effects by effectively inhibiting bacterial growth and disrupting bacterial cell membrane structure.
FOOD AND BIOPROCESS TECHNOLOGY
(2023)
Article
Immunology
Yundong Li, Falin Zhou, Qibin Yang, Song Jiang, Jianhua Huang, Lishi Yang, Zhenhua Ma, Shigui Jiang
Summary: This study used single-cell transcriptome sequencing to construct a single-cell transcriptional map of hepatopancreatic cells and haemocytes in shrimp, and analyzed the molecular mechanisms of the immune response to ammonia nitrogen stress. The results identified the developmental starting points of haemocytes and hepatopancreatic cells, and revealed the activation of antioxidant and immune defense systems under ammonia nitrogen stress. Important response genes played a role in shrimp cell differentiation and functional plasticity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Kai Xu, Weili Wang, Dong Liu, Chao Wang, Jun Zhu, Binlun Yan, Huan Gao, Guangwei Hu
Summary: This study identified a novel crustin-like peptide, EcCrustin2, which was found to be mainly expressed in immune tissues and demonstrated antibacterial activity. These findings suggest that EcCrustin2 plays a crucial role in the immune response against bacteria and EHP infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Ximing Li, Ruixiang Liu, Zhe Wang, Guotai Zheng, Junlin Lv, Lanfen Fan, Yubin Guo, Yuefang Gao
Summary: To enhance farming efficiency, most farmers prefer to purchase Penaeus larvae as opposed to hatching them themselves. However, counting small and highly congested larvae during transactions is a challenging task to accomplish manually. We propose an equal keypoint regression method to improve counting precision and decrease human labor costs.
Article
Fisheries
Meng-Ru Si, Yun-Dong Li, Shi-Gui Jiang, Qi-Bin Yang, Song Jiang, Li-Shi Yang, Jian-Hua Huang, Xu Chen, Fa-Lin Zhou
Summary: In this study, a novel RNA-binding protein, PmCSDE1, was identified in shrimp and found to play a key role in immunological stress regulation. PmCSDE1 was widely expressed in all tissues and downregulated in hepatopancreas and gill tissues under low salt stress. Knockdown of PmCSDE1 resulted in increased shrimp mortality. Additionally, two SNPs in the PmCSDE1 genome were found to be significantly associated with low-salt tolerance. These findings provide a foundation for further research on resistance breeding and the study of low-salt tolerant strains in shrimp.
Article
Multidisciplinary Sciences
Ansaya Pumchan, Sucheewin Krobthong, Sittiruk Roytrakul, Orathai Sawatdichaikul, Hidehiro Kondo, Ikuo Hirono, Nontawith Areechon, Sasimanas Unajak
SCIENTIFIC REPORTS
(2020)
Article
Genetics & Heredity
Ivane R. Pedrosa-Gerasmio, Hidehiro Kondo, Ikuo Hirono
Article
Fisheries
Iyapa Palang, Ikuo Hirono, Saengchan Senapin, Wanna Sirimanapong, Boonsirm Withyachumnarnkul, Rapeepun Vanichviriyakit
JOURNAL OF FISH DISEASES
(2020)
Article
Fisheries
Channapha Sakseepipad, Reiko Nozaki, Keisuke Yoshii, Yutaka Fukuda, Kaori Mizuno, Hidemasa Kawakami, Ikuo Hirono, Hidehiro Kondo
Summary: This study developed two primer sets to amplify SNP regions of RSIV-type megalocytiviruses, identifying 53 polymorphic sites and clustering 25 isolates into eight haplotypes through phylogenetic analysis. These findings suggest that SNPs in the RSIV genome are a powerful tool for detection and identification of RSIV-type megalocytiviruses.
JOURNAL OF FISH DISEASES
(2021)
Article
Genetics & Heredity
Masaya Sato, Satoshi Kawato, Hikaru Oyama, Gen Kaneko, Eric J. Post, Rei Suo, Noriyuki Takai, Haruo Sugita, Hidehiro Kondo, Ikuo Hirono, Shiro Itoi
Summary: The complete mitochondrial genome of the Atlantic Gnomefish was determined, revealing distinct phylogenetic relationships with Japanese and African populations based on the analysis of mitochondrial DNA and cytochrome c oxidase subunit I gene.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Microbiology
Kei Kagaya, Rika Kogure, Masahiko Okai, Satoshi Kawato, Hidehiro Kondo, Ikuo Hirono, Masami Ishida
Summary: This article presents the draft genome sequences of two lipid-degrading bacteria strains, Moritella sp. F1 and F3, isolated from intermediate water in the Sagami Trough, Japan. The genome sizes of these strains are 4,983,334 bp and 4,967,310 bp respectively.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Microbiology
Satoshi Kawato, Reiko Nozaki, Ikuo Hirono, Hidehiro Kondo
Summary: The genome sequence of lymphocystis disease virus 2 LCDV-JP_Oita_2018, isolated from a diseased Japanese flounder in Japan, is presented here. The circular contig of LCDV-JP_Oita_2018 genome is 186,627 bp long, with 140 predicted protein-coding genes and a GC content of 27%.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Genetics & Heredity
Wafaa Ragab, Satoshi Kawato, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
Summary: This study presents a comparative genome analysis of Vibrio penaeicida, revealing its genetic features, evolution, and pathogenicity. The results indicate that the strains belong to the same species and possess typical virulence factors and other related genes. These findings are important for understanding the pathogenesis of Vibrio penaeicida.
MICROBIAL GENOMICS
(2022)
Article
Virology
Satoshi Kawato, Shoya Fujishima, Reiko Nozaki, Hidehiro Kondo, Tadashi Isshiki, Ikuo Hirono
Summary: This study reports the causative agent of milky hemolymph disease in snow crab, known as Chionoecetes opilio bacilliform virus (CoBV), and reveals its complete genome sequence, confirming its classification as a member of the Nimaviridae family.
ARCHIVES OF VIROLOGY
(2023)
Article
Fisheries
Mahmoud Mostafa Mahmoud, Mohamed Abdelsalam, Satoshi Kawato, Shogo Harakawa, Hidemasa Kawakami, Ikuo Hirono, Hidehiro Kondo
Summary: This study compared the whole genome sequences of three different serotypes of Lactococcus garvieae isolated from diseased cultured striped jack in Ehime prefecture, Japan. The serotypes showed different virulence in a challenge test. Two serotypes were identified as L. garvieae and one as L. formosensis. The genome sizes ranged from 1.9 to 2.0 Mb, GC content ranged from 38.2% to 38.9%, and the number of predicted protein-coding sequences ranged from 1922 to 1959. Only one serotype harbored two plasmids.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Jayasundara Mudiyanselage Sajani Nisansala Rathnapala, Wafaa Ragab, Satoshi Kawato, Miho Furukawa, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
Summary: By sequencing the genomes of three strains of Vibrio nigripulchritudo, it was found that they possess various virulence factors and mobile genetic elements, as well as multidrug resistance. Comparative genomic analysis showed that all three strains belong to the same clade.
JOURNAL OF FISH DISEASES
(2023)
Article
Biotechnology & Applied Microbiology
Keiichiro Koiwai, Hidehiro Kondo, Ikuo Hirono
Summary: In this study, Drop-seq technology was used to investigate the impact of virus infection on hemocyte populations in shrimp. The findings revealed that virus infection reduced specific cell populations in circulating hemolymph and inhibited the expression of antimicrobial peptides. Unknown genes with antimicrobial activity were also identified. Additionally, the study discussed the effects of methanol fixation on Drop-seq data.
MARINE BIOTECHNOLOGY
(2023)
Article
Fisheries
Chalermkwan Eurlaphan, Reiko Nozaki, Motohiko Sano, Keiichiro Koiwai, Ikuo Hirono, Hidehiro Kondo
Summary: This study investigated the kinetics of red sea bream iridovirus and host gene expression during infection in rock bream (Oplegnathus fasciatus). The results showed that viral gene transcripts were detected at 3 and 5 days post-injection, and certain host genes were differentially expressed during the infection process. The downregulation of certain inflammation-related genes may contribute to the increased susceptibility of this fish to the virus.
JOURNAL OF FISH DISEASES
(2023)
Article
Microbiology
Satoshi Kawato, Jian Lu, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
Article
Microbiology
Satoshi Kawato, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
Article
Fisheries
Ratiporn Thongsoi, Siriluk Maskaew, Panumas Puechpon, Benjaporn Noppradit, Napassawan Inaek, Prapaporn Utarabhand, Phanthipha Runsaeng
Summary: In this study, a PPAE gene called FmPPAE1 was cloned and analyzed in banana shrimp. FmPPAE1 plays a vital role in regulating the proPO system, exhibits antibacterial properties, and contributes to the suppression of bacterial proliferation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jishuang Tan, Paizelaiti Sahaer, Huan Li, Wei Han, Hongyan Sun
Summary: Circular RNAs (circRNA) are involved in gene expression and affect cellular processes such as transcription, splicing, and translation by interacting with microRNA and RNA binding proteins. A study identified circDNAJB6 as a circular RNA abundant in chicken macrophages after bacterial infection, and found that circDNAJB6 exacerbates cellular injury and interacts with multiple miRNAs and target genes, potentially impacting autophagy and MAPK signaling pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Rodrigo Guabiraba, Damaris Ribeiro Rodrigues, Paul T. Manna, Melanie Chollot, Vincent Saint-Martin, Sascha Trapp, Marisa Oliveira, Clare E. Bryant, Brian J. Ferguson
Summary: The innate immune response relies on the ability of host cells to detect and respond to microbial nucleic acids. Toll-like receptors (TLRs) play a crucial role in this process by distinguishing self from non-self. This study focused on TLR21, an avian TLR that recognizes bacterial DNA motifs. The findings suggest that avian TLR21 shares similar activation mechanisms to mammalian TLR9, highlighting the conservation of nucleic acid sensing mechanisms across species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Safieh Zeinali, Kate Sutton, Lonneke Vervelde
Summary: Chickens have a unique immune structure characterized by the absence of draining lymph nodes and the presence of well-developed mucosa-associated lymphoid tissue. The structure and development of chicken lymphoid tissues in the intestine, particularly Peyer's patches, have been poorly studied. However, the use of CSF1R-eGFP reporter transgenic chickens has allowed for the visualization and investigation of the development and distribution of these tissues. This research provides insights that could benefit mucosal vaccination strategies and the development of targeted vaccine delivery systems.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Retraction
Fisheries
G. -g. Li, Z. -z. Guo, X. -f. Ma, N. Cao, S. -n. Geng, Y. -q. Zheng, M. -j. Meng, H. -h. Lin, G. Han, G. -j. Du
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jinyue Yang, Peng Zhou, Wanrong Wu, Qingxiang Zhang, Dong Chen, Rui Luo
Summary: This study cloned and characterized the full-length sequence of duck MRE11 (duMRE11) for the first time, and found that this protein plays an important role in the innate immune response of ducks.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Mengjiao Guo, Jiaqi Zhang, Mingtao Li, Xiaorong Zhang, Yantao Wu
Summary: rNLRC3 negatively regulates the NF -KB signaling pathway induced by P. multocida in rabbits by inhibiting the activation of NF -KB, reducing the expression of inflammatory cytokines, and interacting with rTRAF4 and rTRAF6. The NACHT-LRR domain is the functional domain of rNLRC3. These findings provide insights into the important role of rNLRC3 in combating P. multocida infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Yinan Li, Xingye Lian, Wenwen Yang, Jinyuan Leng, Jiejie Sun, Yu Liu, Siqi Fan, Lingling Wang, Linsheng Song
Summary: The DM9-containing protein CgDM9CP-7 plays an important role in the innate immune response of molluscs. It is constitutively expressed in various tissues and is up-regulated in haemocytes after stimulation by Vibrio splendidus and lipopolysaccharide (LPS). CgDM9CP-7 binds to various pathogens and exhibits antimicrobial activity, while also regulating the expression of CgMyD88 and CgIL-17.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Xiuwen Bao, Haoran Song, Liying He, Yong Li, Shuqi Niu, Jinlin Guo
Summary: This study focuses on the interaction between Hepialus xiaojinensis larvae and Ophiocordyceps sinensis during the infection process. The results show that the infected third instar larvae enhance antioxidant defense ability, while the infected fourth instar larvae reduce this ability and allocate more energy to growth and development.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
