Article
Immunology
Furong Deng, Di Wang, Thomas P. Loch, Fuguang Chen, Tongyan Lu, Yongsheng Cao, Dan Fan, Shaowu Li
Summary: This study utilized RNA-seq analysis to investigate the molecular mechanism of immune response in rainbow trout spleen during Flavobacterium psychrophilum infection. The results revealed key signaling pathways and provided valuable insights for future research on prevention and control of bacterial coldwater disease in salmon culture.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Jiyeon Park, Hyeongjin Roh, Yoonhang Lee, Ju-Yeop Lee, Hyo-Young Kang, Min Ji Seong, Yura Kang, Young Ung Heo, Bo Seong Kim, Chan-Il Park, Do-Hyung Kim
Summary: This study reports the first detection of Flavobacterium psychrophilum in Korea and describes its genotypic and phenotypic characteristics. The study also reveals the pathogenicity and antibiotic resistance of the pathogen and proposes control measures. This is important for understanding the impact of this pathogen on Korean rainbow trout populations and establishing surveillance and control measures to prevent further transmission and outbreaks.
Article
Fisheries
Jie Ma, Jesse T. Trushenski, Evan M. Jones, Timothy J. Bruce, Doug G. McKenney, Gael Kurath, Kenneth D. Cain
Summary: This study explores the maternal immunity in rainbow trout aquaculture, showing that vaccination of broodstock can reduce mortality in offspring from viral and bacterial diseases.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Juan-Ting Liu, Phuc H. Pham, John S. Lumsden
Summary: Feeding rainbow trout with deoxynivalenol (DON) or partially feeding (pair-fed) can significantly reduce mortality rates when infected with Flavobacterium psychrophilum. Fasted or DON-fed fish showed increased autophagy in the liver, while fasted fish showed decreased autophagy in muscle. Treatment with chloroquine (CQ) increased Atg gene expression in the liver. The mechanisms behind the resistance of rainbow trout fed DON, CQ, or pair-fed to F. psychrophilum infection are still unclear.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Brian W. Avila, Dana L. Winkelman, Eric R. Fetherman
Summary: Research has shown that rainbow trout crosses created by mating resistant strains can exhibit some level of pathogen resistance, but not all crosses show resistance, and only a few multigenerational strains are suitable for further evaluation.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Heidi Mathiessen, Yajiao Duan, Moonika H. Marana, Shaozhi Zuo, Asma M. Karami, Rzgar Jafaar, Louise von Gersdorff Jorgensen, Per W. Kania, Inger Dalsgaard, Lone Madsen, Torben Nielsen, Fabian Grammes, Jorgen odegard, Valeria Macchia, Kurt Buchmann
Summary: Flavobacterium psychrophilum is the causative agent of Bacterial Cold Water Disease (BCWD)/ Rainbow Trout Fry Syndrome (RTFS) in rainbow trout. The disease causes significant mortality in trout farms and leads to economic losses in the aquaculture industry. The immature development of adaptive immunity in young life cycle stages hinders successful vaccination, resulting in the widespread use of antibiotics. Selective breeding and identification of markers associated with disease resistance may improve breeding efforts for more resilient strains.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Di Wu, Shuai Shao, Qin Liu, Qiyao Wang, Yuanxing Zhang, Xiaohong Liu
Summary: Rainbow trout larvae are suffering from high mortality due to infectious hematopoietic necrosis virus (IHNV). In China, IHNV strains have caused significant loss in rainbow trout. A DNA vaccine encoding glycoprotein based on a local IHNV strain was developed, combined with a series of CpG sequences as adjuvant, to control IHNV outbreaks. The vaccine induced both innate and adaptive immune responses, resulting in high survival rate, low virus load, and increased antibody levels in vaccinated fish.
Article
Biotechnology & Applied Microbiology
Segolene Calvez, Nora Navarro-Gonzalez, Charlene Siekoula-Nguedia, Catherine Fournel, Eric Duchaud
Summary: This study assessed the genetic diversity of Flavobacterium psychrophilum isolates from apparently healthy rainbow trout from several fish farms in the same watershed in France. High diversity was revealed by two different genotyping methods, with the majority of isolates being unrelated to the predominant clonal complex worldwide. Thirteen novel sequence types were discovered, suggesting a diverse subpopulation of F. psychrophilum in rainbow trout.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Fisheries
Izzet Burcin Saticioglu, Hilal Ay, Soner Altun, Nevzat Sahin, Muhammed Duman
Summary: In the study of farmed rainbow trout samples in Rize, Turkey, two novel Flavobacterium species were discovered, and their taxonomic positions were confirmed through 16S rRNA gene sequence analysis. A polyphasic taxonomy approach was employed to establish the novelty of these strains, leading to the proposal of Flavobacterium bernardetii sp. nov. The isolates were found to have unique genomic and phenotypic characteristics, indicating a possible correlation with mortality in rainbow trout.
Article
Fisheries
Jason P. Evenhuis, Ryan Lipscomb, Clayton Birkett
Summary: In this study, the virulence phenotypes of two Flavobacterium columnare (Fc) isolates were characterized against rainbow trout at different life stages, revealing stark differences in their pathogenicity towards eyed eggs and alevin. These findings suggest a potential model system for studying virulence kinetics and alternative pathogenic pathways.
JOURNAL OF FISH DISEASES
(2021)
Article
Immunology
Jeremy L. Everson, Darbi R. Jones, Amy K. Taylor, Barb J. Rutan, Timothy D. Leeds, Kate E. Langwig, Andrew R. Wargo, Gregory D. Wiens
Summary: The study found that rainbow trout exposed to reuse water had lower survival rates, while vaccination could reduce the prevalence and load of IHNV and Flavobacterium psychrophilum. Different genetic lines showed variations in response to reuse water.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Mio Takeuchi, Erina Fujiwara-Nagata, Taiki Katayama, Hiroaki Suetake
Summary: Research on manipulating the skin microbiome is important for combating rainbow trout diseases, and using cultivation combined with sequencing helps to identify skin bacteria that can antagonize Flavobacterium psychrophilum.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Jiyeon Park, HyeongJin Roh, Yoonhang Lee, Junewoo Park, Do-Hyung Kim
Summary: The complete genome sequence of Flavobacterium psychrophilum FPRT1 isolated from diseased rainbow trout was reported, with whole-genome sequencing revealing a circular chromosome containing 2,795,347 base pairs and 2,895 protein-coding genes.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Fisheries
Rebecca A. Dupre, Ryan Ardoin, Jesse Trushenski, Chris Jackson, Casey Grimm, Brennan Smith
Summary: The present research demonstrates that dietary exposure is an alternative route of geosmin uptake in Rainbow Trout fillets, leading to a significant increase in geosmin concentrations. This finding is important for further investigation of sensory quality and off-flavor mitigation strategies for farm-raised fish.
Article
Fisheries
Sumanta Kumar Mallik, Shivam Singh, Neetu Shahi, Richa Pathak, Krishna Kala, Partha Das, Bhupendra Singh, Abhay Kumar Giri, Suresh Chandra, Debajit Sarma, Pramod Kumar Pandey
Summary: Flavobacterium species have been found as significant pathogens in aquaculture worldwide. In this study, isolates of Flavobacterium tructae were recovered from infected rainbow trout in the Indian Himalayan Region, and their pathogenicity was investigated. The bacterium showed biofilm formation and haemolytic activity, and it was found to be sensitive to various antibiotics. The study concludes that F.tructae is an opportunistic pathogen and further investigation is needed to evaluate its risk in global aquaculture.
AQUACULTURE INTERNATIONAL
(2023)
Article
Oncology
Daniel E. Carvajal-Hausdorf, Jonathan Patsenker, Kelly P. Stanton, Franz Villarroel-Espindola, Amanda Esch, Ruth R. Montgomery, Amanda Psyrri, Konstantine T. Kalogeras, Vassiliki Kotoula, George Foutzilas, Kurt A. Schalper, Yuval Kluger, David L. Rimm
CLINICAL CANCER RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Kurt A. Schalper, Maria E. Rodriguez-Ruiz, Ricardo Diez-Valle, Alvaro Lopez-Janeiro, Angelo Porciuncula, Miguel A. Idoate, Susana Inoges, Carlos De Andrea, Ascension Lopez-Diaz de Cerio, Sonia Tejada, Pedro Berraondo, Franz Villarroel-Espindola, Jungmin Choi, Alfonso Gurpide, Miriam Giraldez, Iosune Goicoechea, Jaime Gallego Perez-Larraya, Miguel F. Sanmamed, Jose L. Perez-Gracia, Ignacio Melero
Article
Oncology
Arta M. Monjazeb, Kurt A. Schalper, Franz Villarroel-Espindola, Anthony Nguyen, Stephen L. Shiao, Kristina Young
SEMINARS IN RADIATION ONCOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Maria Alejandra Alarcon, Wilda Olivares, Miguel Cordova-Delgado, Matias Munoz-Medel, Tomas de Mayo, Gonzalo Carrasco-Avino, Ignacio Wichmann, Natalia Landeros, Julio Amigo, Enrique Norero, Franz Villarroel-Espindola, Arnoldo Riquelme, Marcelo Garrido, Gareth Owen, Alejandro H. Corvalan
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Oncology
Sandra Martinez-Morilla, Franz Villarroel-Espindola, Pok Fai Wong, Maria Toki, Thazin Nwe Aung, Vasiliki Pelekanou, Brian Bourke-Martin, Kurt A. Schalper, Harriet M. Kluger, David L. Rimm
Summary: The study utilized IMC technology to characterize the tumor microenvironment of patients with metastatic melanoma undergoing immunotherapy, identifying potential indicative biomarkers for treatment response. Through image and multivariable analysis, significant associations with progression-free survival and overall survival were found for specific markers.
CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
Miguel F. Sanmamed, Xinxin Nie, Shruti S. Desai, Franz Villaroel-Espindola, Ti Badri, Dejian Zhao, Anthony W. Kim, Lan Ji, Tianxiang Zhang, Edward Quinlan, Xiaoxiao Cheng, Xue Han, Matthew D. Vesely, Ala F. Nassar, Jingwei Sun, Yu Zhang, Tae Kon Kim, Jun Wang, Ignacio Melero, Roy S. Herbst, Kurt A. Schalper, Lieping Chen
Summary: The study has identified a dysfunctional CD8(+) tumor-infiltrating subpopulation that is highly proliferative, overactivated, and apoptotic, distinct from previously described exhausted T cells. This subpopulation expands in lung cancer tissues in a PD-1/B7-H1-dependent manner and its abundance is associated with resistance to cancer immunotherapy, making it a potential tissue biomarker.
Article
Medicine, General & Internal
Maria Elvira Balcells, Luis Rojas, Nicole Le Corre, Constanza Martinez-Valdebenito, Maria Elena Ceballos, Marcela Ferres, Mayling Chang, Cecilia Vizcaya, Sebastian Mondaca, Alvaro Huete, Ricardo Castro, Mauricio Sarmiento, Luis Villarroel, Alejandra Pizarro, Patricio Ross, Jaime Santander, Barbara Lara, Marcela Ferrada, Sergio Vargas-Salas, Carolina Beltran-Pavez, Ricardo Soto-Rifo, Fernando Valiente-Echeverria, Christian Caglevic, Mauricio Mahave, Carolina Selman, Raimundo Gazitua, Jose Luis Briones, Franz Villarroel-Espindola, Carlos Balmaceda, Manuel A. Espinoza, Jaime Pereira, Bruno Nervi
Summary: The study did not find evidence of benefit in mortality, length of hospitalization, or mechanical ventilation requirement by immediate addition of CP therapy in the early stages of COVID-19 compared to its use only in case of patient deterioration.
Article
Immunology
R. Gonzalez-Stegmaier, K. Cereceda, J. L. Briones, C. Beltran-Pavez, A. Oyarzun-Arrau, S. Riquelme-Barrios, C. Selman, F. Yarad, M. Mahave, C. Caglevic, R. Morales, A. Aguirre, F. Valiente-Echeverria, R. Soto-Rifo, H. Marsiglia, R. Gazitua, F. Villarroel-Espindola
Summary: In Chile, the majority of convalescent patients have developed anti-SARS-CoV-2 antibodies and are suitable candidates for COVID-19 convalescent plasma donation. Neutralizing activity correlates with an increased concentration of anti-RBD IgG and shows variability between donors.
JOURNAL OF IMMUNOLOGY RESEARCH
(2021)
Article
Multidisciplinary Sciences
Carolina Beltran-Pavez, Sebastian Riquelme-Barrios, Aaron Oyarzun-Arrau, Aracelly Gaete-Argel, Roxana Gonzalez-Stegmaier, Karina Cereceda-Solis, Adam Aguirre, Dante Travisany, Ricardo Palma-Vejares, Gonzalo P. Barriga, Aldo Gaggero, Constanza Martinez-Valdebenito, Nicole Le Corre, Marcela Ferres, Maria Elvira Balcells, Jorge Fernandez, Eugenio Ramirez, Franz Villarroel, Fernando Valiente-Echeverria, Ricardo Soto-Rifo
Summary: Chile has one of the worst numbers worldwide in terms of SARS-CoV-2 positive cases and COVID-19-related deaths per million inhabitants; characterization of neutralizing antibody (NAb) responses in the general population is critical to understanding immunity at the local level. The study found samples with decreased or enhanced neutralization activity against the D614G spike variant, indicating its relevance in host immunity. These data provide the first insights into NAb responses in individuals from Chile, serving as a guide for future studies in the country.
Article
Fisheries
Roxana Gonzalez-Stegmaier, Andrea Pena, Franz Villarroel-Espindola, Patricia Aguila, Cristian Oliver, Desmond MacLeod-Carey, Marco Rozas-Serri, Ricardo Enriquez, Jaime Figueroa
Summary: Recombinant flagellin and its D1 domain from Vibrio anguillarum can stimulate pro-inflammatory cytokines in Atlantic salmon, suggesting their potential use as immunostimulants or adjuvants in farmed salmon vaccines.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Oncology
Brian S. Henick, Franz Villarroel-Espindola, Ila Datar, Miguel F. Sanmamed, Jovian Yu, Shruti Desai, Alice Li, Adam Aguirre-Ducler, Konstantinos Syrigos, David L. Rimm, Lieping Chen, Roy S. Herbst, Kurt A. Schalper
Summary: QIF was used to measure the distribution and clinical significance of major myeloid cell subsets in human NSCLC. M1-like macrophages were lower and MDSC-like cells were higher in tumor tissues. Stromal CD11b was higher in SCC than in ADC.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Karina Cereceda, Nicolas Bravo, Roddy Jorquera, Roxana Gonzalez-Stegmaier, Franz Villarroel-Espindola
Summary: This study developed an objective and reliable method for combined biomarker immunostaining and digital image analysis using open informatics tools. The results confirmed the usefulness and robustness of the method in rare cancers.
Article
Biochemistry & Molecular Biology
Roxana Gonzalez-Stegmaier, Adam Aguirre, Constanza Carcamo, Patricia Aguila-Torres, Franz Villarroel-Espindola
Summary: This study evaluated the immunomodulatory effects of a recombinant domain (rND1) from flagellin in human peripheral blood mononuclear cells (PBMCs) and monocyte-derived dendritic cells (MoDCs). It was found that rND1 induced upregulation of proinflammatory cytokines in PBMCs, with IL-1 beta showing the highest increase. MoDCs treated with rND1 showed low expression of co-stimulatory and HLA-DR molecules, indicating an immature phenotype.
Article
Oncology
Vasiliki Pelekanou, Franz Villarroel-Espindola, Kurt A. Schalper, Lajos Pusztai, David L. Rimm
BREAST CANCER RESEARCH
(2018)
Article
Oncology
Franz Villarroel-Espindola, Xiaoqing Yu, Ila Datar, Nikita Mani, Miguel Sanmamed, Vamsidhar Velcheti, Konstantinos Syrigos, Maria Toki, Hongyu Zhao, Lieping Chen, Roy S. Herbst, Kurt A. Schalper
CLINICAL CANCER RESEARCH
(2018)
Article
Fisheries
Ratiporn Thongsoi, Siriluk Maskaew, Panumas Puechpon, Benjaporn Noppradit, Napassawan Inaek, Prapaporn Utarabhand, Phanthipha Runsaeng
Summary: In this study, a PPAE gene called FmPPAE1 was cloned and analyzed in banana shrimp. FmPPAE1 plays a vital role in regulating the proPO system, exhibits antibacterial properties, and contributes to the suppression of bacterial proliferation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jishuang Tan, Paizelaiti Sahaer, Huan Li, Wei Han, Hongyan Sun
Summary: Circular RNAs (circRNA) are involved in gene expression and affect cellular processes such as transcription, splicing, and translation by interacting with microRNA and RNA binding proteins. A study identified circDNAJB6 as a circular RNA abundant in chicken macrophages after bacterial infection, and found that circDNAJB6 exacerbates cellular injury and interacts with multiple miRNAs and target genes, potentially impacting autophagy and MAPK signaling pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Rodrigo Guabiraba, Damaris Ribeiro Rodrigues, Paul T. Manna, Melanie Chollot, Vincent Saint-Martin, Sascha Trapp, Marisa Oliveira, Clare E. Bryant, Brian J. Ferguson
Summary: The innate immune response relies on the ability of host cells to detect and respond to microbial nucleic acids. Toll-like receptors (TLRs) play a crucial role in this process by distinguishing self from non-self. This study focused on TLR21, an avian TLR that recognizes bacterial DNA motifs. The findings suggest that avian TLR21 shares similar activation mechanisms to mammalian TLR9, highlighting the conservation of nucleic acid sensing mechanisms across species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Safieh Zeinali, Kate Sutton, Lonneke Vervelde
Summary: Chickens have a unique immune structure characterized by the absence of draining lymph nodes and the presence of well-developed mucosa-associated lymphoid tissue. The structure and development of chicken lymphoid tissues in the intestine, particularly Peyer's patches, have been poorly studied. However, the use of CSF1R-eGFP reporter transgenic chickens has allowed for the visualization and investigation of the development and distribution of these tissues. This research provides insights that could benefit mucosal vaccination strategies and the development of targeted vaccine delivery systems.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Retraction
Fisheries
G. -g. Li, Z. -z. Guo, X. -f. Ma, N. Cao, S. -n. Geng, Y. -q. Zheng, M. -j. Meng, H. -h. Lin, G. Han, G. -j. Du
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jinyue Yang, Peng Zhou, Wanrong Wu, Qingxiang Zhang, Dong Chen, Rui Luo
Summary: This study cloned and characterized the full-length sequence of duck MRE11 (duMRE11) for the first time, and found that this protein plays an important role in the innate immune response of ducks.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Mengjiao Guo, Jiaqi Zhang, Mingtao Li, Xiaorong Zhang, Yantao Wu
Summary: rNLRC3 negatively regulates the NF -KB signaling pathway induced by P. multocida in rabbits by inhibiting the activation of NF -KB, reducing the expression of inflammatory cytokines, and interacting with rTRAF4 and rTRAF6. The NACHT-LRR domain is the functional domain of rNLRC3. These findings provide insights into the important role of rNLRC3 in combating P. multocida infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Yinan Li, Xingye Lian, Wenwen Yang, Jinyuan Leng, Jiejie Sun, Yu Liu, Siqi Fan, Lingling Wang, Linsheng Song
Summary: The DM9-containing protein CgDM9CP-7 plays an important role in the innate immune response of molluscs. It is constitutively expressed in various tissues and is up-regulated in haemocytes after stimulation by Vibrio splendidus and lipopolysaccharide (LPS). CgDM9CP-7 binds to various pathogens and exhibits antimicrobial activity, while also regulating the expression of CgMyD88 and CgIL-17.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Xiuwen Bao, Haoran Song, Liying He, Yong Li, Shuqi Niu, Jinlin Guo
Summary: This study focuses on the interaction between Hepialus xiaojinensis larvae and Ophiocordyceps sinensis during the infection process. The results show that the infected third instar larvae enhance antioxidant defense ability, while the infected fourth instar larvae reduce this ability and allocate more energy to growth and development.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)