期刊
DEVELOPMENT GROWTH & DIFFERENTIATION
卷 56, 期 9, 页码 615-624出版社
WILEY-BLACKWELL
DOI: 10.1111/dgd.12179
关键词
dental pulp stem cells; insulin-like growth factor 1; osteogenic differentiation; proliferation; rapamycin
资金
- Chinese National Natural Science Foundation [81172841, 81202368, 81471603]
- China Postdoctoral Science Foundation [2013M541708]
- project of 333 Natural Science Foundation of Jiangsu Grant [BRA2013286]
- Top Six Types of Talents Financial Assistance of Jiangsu Province Grant
- Jiangsu Provincial Health Department [Z201005]
- Nantong University [13025043]
- Jiangsu province's Outstanding Medical Academic Leader Program [LJ201136]
Insulin-like growth factor 1 (IGF-1) is a multifunctional peptide that can enhance osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs). However, it remains unclear whether IGF-1 can promote osteogenic differentiation of human dental pulp stem cells (DPSCs). In our study, DPSCs were isolated from the impacted third molars, and treated with IGF-1. Osteogenic differentiation abilities were investigated. We found that IGF-1 activated the mTOR signaling pathway during osteogenic differentiation of DPSCs. IGF-1 also increased the expression of runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osterix (OSX) and collagen type I (COL I) during this process. Rapamycin, an mTOR inhibitor, blocked osteogenic differentiation induced by IGF-1. Meanwhile, CCK-8 assay and flow cytometry results demonstrated that 10-200ng/mL IGF-1 could enhance proliferation ability of DPSCs and 100ng/mL was the optimal concentration. In summary, IGF-1 could promote proliferation and osteogenic differentiation of DPSCs via mTOR pathways, which might have clinical implications for osteoporosis.
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