Biographical-Item
Developmental Biology
Chris Wylie, Claudio D. Stern
Summary: Ruth Bellairs, a legendary figure in chick embryology, pioneered new techniques and made important discoveries. Her mentorship had a profound impact on us.
Article
Chemistry, Multidisciplinary
Elke Hebisch, Martin Hjort, Diogo Volpati, Christelle N. Prinz
Summary: Fluorescent nanodiamonds (FND) are promising materials for long term cell labeling and tracking due to their stable fluorescence, biocompatibility, and amenability to functionalization. This study successfully achieved direct delivery of FND to the cytosol using nanostraws and low voltage electroporation pulses, showing efficient and rapid transport of FND into cells. Live cell STED microscopy revealed that cell membranes open entirely on top of nanostraws, supporting the hypothesis that many FND are delivered directly to the cytosol, avoiding endocytosis and lysosomal entrapment.
Article
Biology
Masahito Yamagata, Joshua R. Sanes
Summary: The ability to mark and manipulate specific cell types is essential for various analyses in model organisms. This study introduces a method for CRISPR-based somatic integration of reporters or Cre recombinase into specific genes in the chick genome, allowing for visualization of cells in the retina and midbrain. Genomic loci are selected based on an RNA-seq-based cell atlas, and reporters can be used for visualizing cell morphology or assessing subcellular localization.
Review
Immunology
Paul Garcia, Yan Wang, Jean Viallet, Zuzana Macek Jilkova
Summary: The chicken embryo model has emerged as an alternative to rodents in immune-based studies, offering advantages such as cost-effectiveness, time efficiency, and ease of use. It can be utilized for specific experimental hypotheses or preclinical testing, potentially serving as a valuable tool in biomedical research.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Xi Sun, Yumeng Wang, Chaohui Wang, Yibin Wang, Zhouzheng Ren, Xin Yang, Xiaojun Yang, Yanli Liu
Summary: The aim of this study was to reveal the epigenetic mechanism of hepatic tran-scriptional reprogramming changes during the embryonic period of chicken. The results showed gradual increase in lipid contents and decline in H3K27ac and H3K4me2 levels with embryonic age. Differential expression genes (DEGs) were determined through transcriptome analysis. Furthermore, the integration of RNA-seq and CUT&Tag data revealed the involvement of H3K27ac histone modification in transcriptional reprogramming regulation and the recruitment of transcription factors (TFs) to mediate differential enhancer activation.
Article
Biochemistry & Molecular Biology
Yunfeng Zhang, Lingyu Xie, Xiaoqing Ding, Yuanyuan Wang, Yibin Xu, Danlei Li, Shuang Liang, Yongxia Wang, Ling Zhang, Aikun Fu, Xiuan Zhan
Summary: This study found that maternal zinc can alleviate oxidative stress injury caused by heat stress in chicken embryos. It enhances antioxidant capacity, reduces apoptosis, and activates the Nrf2 signaling pathway.
Review
Genetics & Heredity
Jason C. Lin, Alison L. Van Eenennaam
Summary: Introducing genome editing reagents into mammalian zygotes via electroporation provides a simpler and more streamlined approach compared to traditional methods. Studies have examined parameters for mouse and rat zygotes, with limited reports on livestock zygotes. Delivery of genome editing reagents via electroporation can help reduce mosaicism, especially in large livestock species.
FRONTIERS IN GENETICS
(2021)
Article
Agriculture, Dairy & Animal Science
Ahmad Hanafi Sulong, Idrus Zulkifli, Suriya Kumari Ramiah, Eric Lim Teik Chung, Elmutaz Atta Awad, Awis Qurni Sazili
Summary: The study detected APPs in the blood of chicken embryos and neonates for the first time, with levels varying depending on the developmental stage.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sakura Niinuma, Yui Wake, Yuki Nakagawa, Takehito Kaneko
Summary: By evaluating the localization of Cas9 protein with or without nuclear localization signal (NLS) in mouse embryos, we found that the effective transportation of Cas9 from the cytoplasm to the pronuclei through NLS resulted in increased genome-editing rates in offspring.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Developmental Biology
Ido Regev, Karine Guevorkian, Anupam Gupta, Olivier Pourquie, L. Mahadevan
Summary: This study investigates the effects of cell motility gradients on body elongation using the chicken embryo as a model. The combination of electroporation and time-lapse imaging techniques reveals that random cell motility induced by a cellular signal (FGF) and geometric confinement play important roles in tissue elongation. Both experimental observations and theoretical models demonstrate that the continuous addition of cells at the tail bud, combined with lateral confinement, results in directed movement and body elongation.
Article
Agriculture, Dairy & Animal Science
Yu Zhang, Bu-ting Duan, Yong-juan Zhao, Kai-ling Cui, Tong Xu, Xue-song Zhang, Xiao-ling Lv, Lu-Lu Guo, Ming-xue Zheng, Rui Bai
Summary: Eimeria tenella infection induces autophagy in chicken embryo cecal epithelial cells. The expression of autophagy-related proteins is increased, while p62 protein levels are decreased. Chloroquine enhances autophagy, reducing E. tenella infection, while rapamycin induces autophagy and inhibits E. tenella infection. In contrast, 3-Methyladenine treatment increases E. tenella infection rate.
Article
Agriculture, Dairy & Animal Science
Xujie Li, Nancy McLean, Janice MacIsaac, Alex Martynenko, Bruce Rathgeber
Summary: This study investigates the effects of photoperiod and strain on the temperature, hatching traits, and growth performance of broiler embryos. Results show that providing 12-hour or 18-hour light during incubation lowers air cell temperature compared to no illumination. Different strains of embryos respond differently to periodic illumination. The 12-hour light treatment improves chick quality at hatch and results in heavier birds compared to the 18-hour light treatment.
Article
Agriculture, Dairy & Animal Science
Ozlem Varol Avcilar, Esin Ebru Onbasilar, Sakine Yalcin, Hanife Akca, Umut Can Gundogar
Summary: It is important to consider the breeder age when obtaining chicks for broiler fattening, as it affects the mineral levels in eggs and bones. This study aimed to examine the changes in mineral levels, embryonic development, and bone characteristics according to breeder age. The results showed that older breeders produced eggs with higher weight and embryos with greater weight and length. The mineral levels in the shell, albumen, yolk, and bones of embryos and chicks were also higher in the older breeders. This information can be useful in designing in-ovo injections, formulating diets, and preventing bone diseases in broilers.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2023)
Article
Multidisciplinary Sciences
Samuel V. Rasmussen, Noah E. Berlow, Lisa Hudson Price, Atiya Mansoor, Stefano Cairo, Sandra Rugonyi, Charles Keller
Summary: Preclinical cancer research can be expensive, with in vitro studies often not representative of the tumor microenvironment and mouse studies being costly at scale. Using Japanese quail in chorioallantoic membrane (CAM) assays, researchers have developed a cost-effective method to screen for anti-cancer efficacy and drug toxicity, demonstrating concordance with murine in vivo studies and suggesting potential for validating new anti-cancer agents.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Kelly Harper, Anna Yatsyna, Martine Charbonneau, Karine Brochu-Gaudreau, Alexis Perreault, Claudio Jeldres, Patrick P. McDonald, Claire M. Dubois
Summary: Hypoxia in the tumor microenvironment is a negative prognostic factor associated with tumor progression and metastasis, making it an attractive therapeutic target for anti-tumor therapy. The use of the CAM model effectively supports the development of hypoxic zones in a variety of tumor types, offering a valuable platform for studying hypoxia and testing drugs targeting this tumor microenvironment.