期刊
DEVELOPMENT GENES AND EVOLUTION
卷 221, 期 5-6, 页码 357-362出版社
SPRINGER
DOI: 10.1007/s00427-011-0379-z
关键词
Heat shock proteins; Metamorphosis; Eye development; RNA interference; Tribolium castaneum
资金
- Hessian Ministry of Science and Art (HMWK) via the LOEWE
Heat shock protein 90 (HSP90) belongs to a family of conserved chaperons with multiple roles in stress adaptation and development, including spermatogenesis, oogenesis and embryogenesis in insects. In the red flour beetle, Tribolium castaneum, we found that HSP90 is transiently upregulated during larval development, in prepupae, in female pupae and in adults, suggesting multiple post-embryonic roles. We found that silencing HSP90 expression by RNA interference was lethal within 10 days at all developmental stages. Titration experiments revealed that larvae were more susceptible than pupae or beetles. Interestingly, HSP90 silencing in final instar larvae resulted in abnormal pupal phenotypes lacking compound eyes and exhibiting prepupal features, suggesting developmental arrest at the prepupal stage. Our results suggest that HSP90 functions can be expanded beyond the known ones in insect embryogenesis to include roles in post-embryonic development such as the regulation of compound eye development.
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