期刊
DEVELOPMENT
卷 141, 期 10, 页码 2157-2164出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.105148
关键词
LCN2; Sperm; Lipid raft; Phosphatidylethanolamine; PKA; Oviduct; Mouse
资金
- Ministry of Education, Science, Sports and Culture of Japan
- Fujiwara Foundation
Mammalian sperm undergo multiple maturation steps after leaving the testis in order to become competent for fertilization, but the molecular mechanisms underlying this process remain unclear. In terms of identifying factors crucial for these processes in vivo, we found that lipocalin 2 (Lcn2), which is known as an innate immune factor inhibiting bacterial and malarial growth, can modulate sperm maturation. Most sperm that migrated to the oviduct of wild-type females underwent lipid raft reorganization and glycosylphosphatidylinositol-anchored protein shedding, which are signatures of sperm maturation, but few did so in Lcn2 null mice. Furthermore, we found that LCN2 binds to membrane phosphatidylethanolamine to reinforce lipid raft reorganization via a PKA-dependent mechanism and promotes sperm to acquire fertility by facilitating cholesterol efflux. These observations imply that mammals possess a mode for sperm maturation in addition to the albumin-mediated pathway.
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