期刊
DEVELOPMENT
卷 141, 期 14, 页码 2901-2911出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.106898
关键词
Epithelial morphogenesis; Junctional dynamics; Mechanical forces; Quantitative image analysis; Segmentation algorithms; Wound healing; Drosophila
资金
- Ontario Trillium Scholarship
- Connaught Fund New Investigator Award
- University of Toronto Faculty of Medicine Dean's New Staff Fund
- Canada Foundation for Innovation [30279]
- Ontario Research Fund
- Natural Sciences and Engineering Research Council of Canada Discovery Grant [418438-13]
The embryonic epidermis displays a remarkable ability to repair wounds rapidly. Embryonic wound repair is driven by the evolutionary conserved redistribution of cytoskeletal and junctional proteins around the wound. Drosophila has emerged as a model to screen for factors implicated in wound closure. However, genetic screens have been limited by the use of manual analysis methods. We introduce MEDUSA, a novel image-analysis tool for the automated quantification of multicellular and molecular dynamics from time-lapse confocal microscopy data. We validate MEDUSA by quantifying wound closure in Drosophila embryos, and we show that the results of our automated analysis are comparable to analysis by manual delineation and tracking of the wounds, while significantly reducing the processing time. We demonstrate that MEDUSA can also be applied to the investigation of cellular behaviors in three and four dimensions. Using MEDUSA, we find that the conserved nonreceptor tyrosine kinase Abelson (Abl) contributes to rapid embryonic wound closure. We demonstrate that Abl plays a role in the organization of filamentous actin and the redistribution of the junctional protein beta-catenin at the wound margin during embryonic wound repair. Finally, we discuss different models for the role of Abl in the regulation of actin architecture and adhesion dynamics at the wound margin.
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