期刊
DEVELOPMENT
卷 141, 期 24, 页码 4841-4850出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.113365
关键词
Polysaccharides; Arabidopsis; Exoskeletons; Carbohydrate microarrays; Cell wall; Fluorescence imaging; Root cap
资金
- FP7 Marie Curie action project CeWalDyn [329830]
- Danish National Advanced Technology Foundation project [B21st]
- Danish Foundation for Strategic Research
- Norwegian Research Council [214613]
- FP7 project Waste2Go [308363]
- US National Science Foundation (NSF) Molecular and Cellular Bioscience [0919925]
- NSF Division of Biological Infrastructure [0922805]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1337280, 0922805] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0919925] Funding Source: National Science Foundation
Polysaccharides are major components of extracellular matrices and are often extensively modified post-synthetically to suit local requirements and developmental programmes. However, our current understanding of the spatiotemporal dynamics and functional significance of these modifications is limited by a lack of suitable molecular tools. Here, we report the development of a novel non-immunological approach for producing highly selective reciprocal oligosaccharide-based probes for chitosan (the product of chitin deacetylation) and for demethylesterified homogalacturonan. Specific reciprocal binding is mediated by the unique stereochemical arrangement of oppositely charged amino and carboxy groups. Conjugation of oligosaccharides to fluorophores or gold nanoparticles enables direct and rapid imaging of homogalacturonan and chitosan with unprecedented precision in diverse plant, fungal and animal systems. We demonstrated their potential for providing new biological insights by using them to study homogalacturonan processing during Arabidopsis thaliana root cap development and by analyzing sites of chitosan deposition in fungal cell walls and arthropod exoskeletons.
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