Article
Multidisciplinary Sciences
Michael E. Baumgartner, Paul F. Langton, Remi Logeay, Alex Mastrogiannopoulos, Anna Nilsson-Takeuchi, Iwo Kucinski, Jules Lavalou, Eugenia Piddini
Summary: Investigating organ biology often involves creating genetically distinct clones in living tissues. However, analyzing the 3D nature of these clones is challenging and time-consuming, leading to limited manually-extracted information. In this study, the researchers introduce PECAn, a pipeline for processing and analyzing complex multi-genotype 3D images, which enables rapid and scalable rigorous analyses without programming skills. They demonstrate the effectiveness of PECAn by applying it to study Minute cell competition in drosophila, identifying sexual dimorphism and tissue parameters correlated with competitive death, as well as discovering genes involved in cell competition through an RNAi-based screen.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Frank Macabenta, Hsuan-Te Sun, Angelike Stathopoulos
Summary: Through regulating the FGF and BMP signaling pathways, collectively migrating Drosophila muscle precursors promote cell homeostasis and prevent anoikis. The BMP signaling pathway drives cell-cycle regulation and apoptosis to maintain tissue homeostasis.
DEVELOPMENTAL CELL
(2022)
Article
Multidisciplinary Sciences
A. Raouf Issa, Jonathan A. C. Menzies, Aishwarya Padmanabhan, Claudio R. Alonso
Summary: This study reveals a new role of Hox genes in controlling adult behavior and neural function. By studying Drosophila, it was found that downregulating Hox genes in adult neurons leads to flight anomalies. Further investigation shows that Hox genes are required within a subset of dopaminergic neurons, and their role in flight control is linked through cell circuitry analysis and optogenetics.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Sophie Layalle, Laetitia They, Sarah Ourghani, Cedric Raoul, Laurent Soustelle
Summary: Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease characterized by the degeneration of motoneurons. Fruit flies have emerged as a versatile model for studying ALS, providing insights into cellular mechanisms and potential therapeutic targets for future treatments. Research on fruit fly ALS models has revealed novel pathogenic mechanisms and identified disease-modifying genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Stefanny Villalobos-Cantor, Ruth M. Barrett, Alec F. Condon, Alicia Arreola-Bustos, Kelsie M. Rodriguez, Michael S. Cohen, Ian Martin
Summary: Controlled protein synthesis is crucial for regulating gene expression in a cell type-specific manner. We developed a new method to specifically label and visualize newly-synthesized proteins in targeted cell populations in the fly brain, allowing for quantitative analysis of protein synthesis states in vivo.
Article
Cell Biology
Michael E. Baumgartner, Michael P. Dinan, Paul F. Langton, Iwo Kucinski, Eugenia Piddini
Summary: Baumgartner et al. identify proteotoxic stress as the underlying cause of the loser status in a cell competition model caused by reduced autophagic, proteasomal flux and accumulation of protein aggregates.
NATURE CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jeroen Dobbelaere, Tiffany Y. Su, Balazs Erdi, Alexander Schleiffer, Alexander Dammermann
Summary: Cilia are cellular projections that perform sensory and motile functions in eukaryotic cells. In this study, a set of 386 human genes associated with cilium assembly or motility were identified by analyzing the presence and absence pattern in the genomes of diverse eukaryotes. The novel genes were further characterized and found to be related to cilium defects in fruit flies and nematodes. This dataset defines the core set of genes required for cilium assembly and motility across eukaryotes and provides a valuable resource for future studies.
Article
Environmental Sciences
Zhi Chen, Fen Wang, Di Wen, Ren Mu
Summary: This study investigates the gastrointestinal toxicity of Bisphenol A (BPA) in the fruit fly model organism Drosophila melanogaster. The results show that BPA exposure causes morphological changes in the midgut of the fruit flies, as well as a decrease in survival rates and climbing ability. BPA also induces oxidative stress in the midgut, leading to cell death and gut barrier dysfunction. Additionally, BPA-induced oxidative injury stimulates the proliferation of intestinal stem cells and tissue regeneration. The activation of the Jun N-terminal kinase signal pathway is required for BPA-induced cell death and tissue regeneration in the midgut.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Cell Biology
Di Wen, Zhi Chen, Jiamin Wen, Qiangqiang Jia
Summary: In Drosophila melanogaster, different dietary sterols have differential effects on the expression of 20E biosynthetic genes and activate 20E primary response gene expression. Single sterol types are sufficient to support growth and development. These findings suggest that the enzymatic system responsible for the classical C-27 ecdysteroid biosynthetic pathway also participates in the conversion of C-28 and C-29 dietary sterols into C-28 ecdysteroids.
Article
Multidisciplinary Sciences
Sreesankar Easwaran, Matthew Van Ligten, Mackenzie Kui, Denise J. Montell
Summary: This study reveals that Drosophila enter adult reproductive diapause under specific environmental conditions, which leads to ovarian arrest but preserves fertility. Germline stem cells (GSCs) in diapause incur DNA damage and division reduction, yet they are able to recover. Mimicking reduced juvenile hormone production during diapause enhances GSC longevity in non-diapausing flies.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
U. Nguyen, B. Tinsley, Y. Sen, J. Stein, Y. Palacios, A. Ceballos, C. Welch, K. Nzenkue, A. Penn, L. Murphy, K. Leodones, J. Casiquin, I. Ivory, K. Ghenta, K. Danziger, E. Widman, J. Newman, M. Triplehorn, Z. Hindi, K. Mulligan
Summary: Research investigated the neurodevelopmental impacts of BPA on fruit flies across different genetic strains, showing that BPA can have varying effects depending on genetic background, indicating a potential gene-environment interaction.
Review
Biochemistry & Molecular Biology
Arjun Rajan, Cyrina M. Ostgaard, Cheng-Yu Lee
Summary: Indirect neurogenesis, driven by intermediate progenitors, plays a crucial role in brain evolution. Studying the mechanisms in fruit fly neural stem cells has provided valuable insights into the regulation of indirect neurogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ekaterina Proshkina, Elena Yushkova, Liubov Koval, Nadezhda Zemskaya, Evgeniya Shchegoleva, Ilya Solovev, Daria Yakovleva, Natalya Pakshina, Natalia Ulyasheva, Mikhail Shaposhnikov, Alexey Moskalev
Summary: Small RNAs play a crucial role in coordinating various cellular processes, affecting gene expression, genomic stability, and suppressing mutagenic transposon activity, thus influencing aging, longevity, and sensitivity to stress factors. Proteins of the Argonaute family are essential for the biogenesis and activity of small RNAs, but their role in regulating lifespan and radioresistance is still not well understood. Studies on knockdown of Argonaute genes in different tissues of Drosophila melanogaster show tissue-specific effects on lifespan, survival after irradiation, and retrotransposon levels, providing insights into the associated molecular mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Rohit Joshi, Rashmi Sipani, Asif Bakshi
Summary: Hox genes play a crucial role in determining the anterior-posterior axis in animal body plans. They are involved in neurogenesis and myogenesis, and also control the assembly and function of neuromuscular networks, ultimately affecting behavior.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Jiaqi Li, Jingjing Wang, Peijing Zhang, Renying Wang, Yuqing Mei, Zhongyi Sun, Lijiang Fei, Mengmeng Jiang, Lifeng Ma, E. Weigao, Haide Chen, Xinru Wang, Yuting Fu, Hanyu Wu, Daiyuan Liu, Xueyi Wang, Jingyu Li, Qile Guo, Yuan Liao, Chengxuan Yu, Danmei Jia, Jian Wu, Shibo He, Huanju Liu, Jun Ma, Kai Lei, Jiming Chen, Xiaoping Han, Guoji Guo
Summary: This study generated single-cell whole-body expression landscapes of zebrafish, Drosophila, and earthworm using Microwell-seq. By integrating cell landscapes from eight representative species, the researchers developed a deep-learning-based strategy, Nvwa, to predict gene expression and identify regulatory programs. Conserved genetic regulation in vertebrates and invertebrates were revealed through comparison of cell-type-specific transcription factors. This work provides a valuable resource and a new strategy for studying regulatory grammar in diverse biological systems.
Article
Neurosciences
Jonathan Enriquez, Lalanti Venkatasubramanian, Myungin Baek, Meredith Peterson, Ulkar Aghayeva, Richard S. Mann
Article
Neurosciences
Jonathan Enriquez, Laura Quintana Rio, Richard Blazeski, Stephanie Bellemin, Pierre Godement, Carol Mason, Richard S. Mann
Article
Developmental Biology
Jonathan Enriquez, Hadi Boukhatmi, Laurence Dubois, Anthony A. Philippakis, Martha L. Bulyk, Alan M. Michelson, Michele Crozatier, Alain Vincent
Article
Developmental Biology
Hadi Boukhatmi, Jean Louis Frendo, Jonathan Enriquez, Michele Crozatier, Laurence Dubois, Alain Vincent
Article
Developmental Biology
Jonathan Enriquez, Mathilde de Taffin, Michele Crozatier, Alain Vincent, Laurence Dubois
DEVELOPMENTAL BIOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Jonathan Enriquez, Alain Vincent
Article
Multidisciplinary Sciences
Wenyue Guan, Lalanti Venkatasubramanian, Myungin Baek, Richard S. Mann, Jonathan Enriquez
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Multidisciplinary Sciences
H. Babski, T. Jovanic, C. Surel, S. Yoshikawa, M. F. Zwart, J. Valmier, J. B. Thomas, J. Enriquez, P. Carroll, A. Garces
NATURE COMMUNICATIONS
(2019)
Article
Medicine, Research & Experimental
Camille Guillermin, Mathilde Bouchet, Alain Garces, Jonathan Enriquez
M S-MEDECINE SCIENCES
(2020)
Article
Developmental Biology
Laurence Dubois, Jonathan Enriquez, Virginie Daburon, Fabien Crozet, Gaelle Lebreton, Michele Crozatier, Alain Vincent
