Article
Cell & Tissue Engineering
Kasey Y. C. Lau, Hernan Rubinstein, Carlos W. Gantner, Ron Hadas, Gianluca Amadei, Yonatan Stelzer, Magdalena Zernicka-Goetz
Summary: Scientists have developed an in vitro model using mouse embryonic stem cells (ESCs) that can recapitulate mouse embryogenesis, including the interactions between embryonic and extraembryonic tissues. By comparing the transcriptional programs with natural embryos, the model shows remarkable similarity but also some divergences.
Article
Biochemistry & Molecular Biology
Jianmin Hu, Jin Li, Can Dai, Jinlin Ren, Wenru Yang, Caixia He, Fei Meng, Congling Dai, Sicong Zeng
Summary: By introducing HES1-/-mutations into human embryonic stem cells and differentiating them into human intestinal organoids, the study reveals the important role of HES1 and WNT5A signaling in epithelial-mesenchymal crosstalk in the human intestinal mucosa.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Developmental Biology
Simone Probst, Sagar, Jelena Tosic, Carsten Schwan, Dominic Grun, Sebastian J. Arnold
Summary: Research shows that in mouse embryo development, progenitors of anterior mesoderm and definitive endoderm differentiate specifically in the primitive streak under the control of the Eomes signal. Through genetic experiments and single-cell RNA sequencing, the relationships and differentiation trajectories of these cell types are revealed.
Article
Cell Biology
Katrin M. Schuele, Jelena Weckerle, Simone Probst, Alexandra E. Wehmeyer, Lea Zissel, Chiara M. Schroeder, Mehmet Tekman, Gwang-Jin Kim, Inga-Marie Schlaegl, Sagar, Sebastian J. Arnold
Summary: Eomes and Brachyury exhibit distinct transcriptional activities, guiding the specification of specific cell lineages. While their binding sites overlap at the promoters of target genes, they show specificity for distal enhancer regions.
DEVELOPMENTAL CELL
(2023)
Article
Multidisciplinary Sciences
Lizhong Liu, Anastasiia Nemashkalo, Luisa Rezende, Ji Yoon Jung, Sapna Chhabra, M. Cecilia Guerra, Idse Heemskerk, Aryeh Warmflash
Summary: This study visualizes the activity of endogenous Nodal protein in living cells using a human gastruloid model and reveals that Nodal activity spreads through a relay mechanism, with timing controlled by the Nodal inhibitor Lefty. This study provides insights into how morphogen gradients are formed and interpreted during mammalian embryogenesis.
NATURE COMMUNICATIONS
(2022)
Review
Biology
Karen M. Downs
Summary: This article discusses the formation and function of the placental umbilical cord and the development of the allantois in placental mammals. It provides new insights into the mechanism and role of the allantois, and highlights its importance in understanding various diseases in humans.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Cell Biology
Gianluca Amadei, Kasey Y. C. Lau, Joachim De Jonghe, Carlos W. Gantner, Berna Sozen, Christopher Chan, Meng Zhu, Christos Kyprianou, Florian Hollfelder, Magdalena Zernicka-Goetz
Summary: The study demonstrates that replacing XEN cells with ESCs transiently expressing Gata4 enhances the developmental potential of iETX embryos, enabling the study of anterior-posterior patterning and gastrulation in an in vitro system.
DEVELOPMENTAL CELL
(2021)
Article
Chemistry, Medicinal
Yuta Hozumi, Kiyoto Aramis Tanemura, Guo-Wei Wei
Summary: Single-cell RNA sequencing (scRNA-seq) is commonly used to study cellular heterogeneity and has great potential for understanding cell-cell communication, cell differentiation, and gene expression. However, analyzing scRNA-seq data is challenging due to sparsity and high dimensionality. In this study, we introduce a new dimensionality reduction method called Correlated Clustering and Projection (CCP) that has advantages over traditional methods like principal component analysis (PCA). We also propose a novel metric called Residue-Similarity index (RSI) for clustering and classification and a new visualization tool called the R-S plot for data with a large number of cell types.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Gastroenterology & Hepatology
Saiko Marui, Yoshihiro Nishikawa, Masahiro Shiokawa, Masataka Yokode, Shimpei Matsumoto, Yuya Muramoto, Sakiko Ota, Takeharu Nakamura, Hiroyuki Yoshida, Hirokazu Okada, Takeshi Kuwada, Tomoaki Matsumori, Katsutoshi Kuriyama, Akihisa Fukuda, Dieter Saur, Takashi Aoi, Norimitsu Uza, Yuzo Kodama, Tsutomu Chiba, Hiroshi Seno
Summary: The roles of HES1 in the adult pancreas and pancreatic cancer are context-dependent. HES1 is not required for maintaining the adult pancreas under normal conditions, but is important for regeneration during recovery from pancreatitis. Furthermore, HES1 promotes early lesion formation while inhibiting the progression of late lesions in pancreatic cancer.
Article
Cell Biology
Vivian Lu, Irena J. Roy, Alejandro Torres, James H. Joly, Fasih M. Ahsan, Nicholas A. Graham, Michael A. Teitell
Summary: This study reveals that, under exogenous glutamine withdrawal, ectoderm can generate sufficient glutamine to sustain cell viability and differentiation, while glutamine in mesoderm and endoderm is a preferred precursor of other compounds. The study also suggests that factors affecting germ layer differentiation may include specific glutamine enzyme gene expression patterns at the embryonic stage.
DEVELOPMENTAL CELL
(2022)
Article
Cell Biology
Valeriya V. Zinina, Melanie Sauer, Lira Nigmatullina, Nastasja Kreim, Natalia Soshnikova
Summary: This study investigated the role of TCF7L1 in the embryonic and adult intestinal epithelium using conditional mouse mutants. The results showed that TCF7L1 prevents the precocious differentiation of embryonic intestinal epithelial progenitors towards enterocytes and ISCs. In addition, TCF7L1 is necessary for the differentiation of secretory epithelial progenitors along the tuft cell lineage and for the differentiation of enteroendocrine D- and L-cells in the anterior small intestine. The study concludes that TCF7L1-mediated repression of both Notch and WNT pathways is crucial for the correct differentiation of intestinal secretory progenitors.
Review
Cardiac & Cardiovascular Systems
Wai Hoe Ng, Barbie Varghese, Hongpeng Jia, Xi Ren
Summary: Studies have shown that signals from adjacent endodermal tissues play a crucial role in coordinating proper cardiac morphogenesis. In vitro models like cardiac organoids have potential, but cannot capture the complex communication between the heart and endodermal organs. Recent reports of multilineage organoids combining cardiac and endodermal derivatives have shed light on their morphogenesis and shared signaling requirements. These organoids offer insights into human development and could be used for regenerative interventions, disease investigation, and drug testing.
CIRCULATION RESEARCH
(2023)
Article
Cell & Tissue Engineering
Lide Su, Guofu Zhang, Lili Jiang, Chao Chi, Bing Bai, Kai Kang
Summary: By knocking out the c-Jun gene in mouse embryonic stem cells using CRISPR-Cas9, it was found that the resulting cells were directed towards a meso-endodermal fate, leading to the generation of beating myocardial cells. This suggests that c-Jun plays a significant role in regulating early cardiac cell development.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Kulbhushan Sharma, Nagham T. Asp, Sean P. Harrison, Richard Siller, Saphira F. Baumgarten, Swapnil Gupta, Maria E. Chollet, Elisabeth Andersen, Gareth J. Sullivan, Anne Simonsen
Summary: This study investigates the role of autophagy in early events of lineage commitment using human embryonic and induced pluripotent stem cells. They found that modulation of autophagy affects SOX2 levels and lineage commitment, with autophagy promoting mesendoderm formation and inhibiting neuroectoderm formation. The results suggest that autophagy-mediated SOX2 turnover is a key factor in determining lineage commitment.
Review
Endocrinology & Metabolism
Federica Marelli, Giuditta Rurale, Luca Persani
Summary: This review provides an update on recent advancements in understanding the early steps of thyroid differentiation in zebrafish, highlighting the physiological processes and molecular mechanisms involved. The study reveals a complex crosstalk between intrinsic and extrinsic factors that are crucial for proper thyroid development, with various signaling pathways playing important roles in the commitment of endodermal cells to a thyroid fate during specific developmental windows in the zebrafish embryo.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Ke Liu, Lei Lyu, David Chin, Junyuan Gao, Xiurong Sun, Fu Shang, Andrea Caceres, Min-Lee Chang, Sheldon Rowan, Junmin Peng, Richard Mathias, Hideko Kasahara, Shuhong Jiang, Allen Taylor
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Ophthalmology
Sheldon Rowan, Min-Lee Chang, Natalie Reznikov, Allen Taylor
EXPERIMENTAL EYE RESEARCH
(2017)
Article
Multidisciplinary Sciences
Sheldon Rowan, Shuhong Jiang, Tal Korem, Jedrzej Szymanski, Min-Lee Chang, Jason Szelog, Christa Cassalman, Kalavathi Dasuri, Christina McGuire, Ryoji Nagai, Xue-Liang Du, Michael Brownlee, Naila Rabbani, Paul J. Thornalley, James D. Baleja, Amy A. Deik, Kerry A. Pierce, Justin M. Scott, Clary B. Clish, Donald E. Smith, Adina Weinberger, Tali Avnit-Sagi, Maya Lotan-Pompan, Eran Segal, Allen Taylor
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Biochemistry & Molecular Biology
Chung-Jung Chiu, Naila Rabbani, Sheldon Rowan, Min-Lee Chang, Sherilyn Sawyer, Frank B. Hu, Walter Willett, Paul J. Thornalley, Attia Anwar, Liliana Bar, Jae H. Kang, Allen Taylor
Review
Biochemistry & Molecular Biology
Sheldon Rowan, Eloy Bejarano, Allen Taylor
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2018)
Article
Multidisciplinary Sciences
Raymond M. Anchan, Salil A. Lachke, Behzad Gerami-Naini, Jennifer Lindsey, Nicholas Ng, Catherine Naber, Michael Nickerson, Resy Cavallesco, Sheldon Rowan, Jennifer L. Eaton, Qiongchao Xi, Richard L. Maas
Article
Genetics & Heredity
Phuong T. Lam, Stephanie L. Padula, Thanh V. Hoang, Justin E. Poth, Lin Liu, Chun Liang, Adam S. LeFever, Lindsay M. Wallace, Ruth Ashery-Padan, Penny K. Riggs, Jordan E. Shields, Ohad Shaham, Sheldon Rowan, Nadean L. Brown, Tom Glaser, Michael L. Robinson
Article
Gastroenterology & Hepatology
Sheldon Rowan, Allen Taylor
Meeting Abstract
Ophthalmology
Sheldon Rowan, Shuhong Jiang, Carlos Moreira-Neto, Min-Lee Chang, Donald Smith, Nadia K. Waheed, Allen Taylor
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2018)
Meeting Abstract
Ophthalmology
Sheldon Rowan, Shuhong Jiang, Min-Lee Chang, Naila Rabbani, Paul J. Thornalley, Donald Smith, Allen Taylor
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2017)
Meeting Abstract
Ophthalmology
Allen Taylor, Sheldon Rowan, Min-Lee Chang, Fu Shang, Natalie Reznikov, Lei Lyu, Ke Liu
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2016)
Meeting Abstract
Ophthalmology
Sheldon Rowan, Shuhong Iang, Min-Lee Chang, Ledrzej Szymanski, Tal Korem, Eran Segal, Christa Cassalman, Christina McGuire, James D. Balea, Clary B. Clish, Allen Taylor
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2016)
Meeting Abstract
Ophthalmology
Sheldon Rowan, Shuhong Jiang, Min-Lee Chang, Christa Cassalman, Christina McGuire, James D. Baleja, Barbara A. Nagel, Steven J. Fliesler, Donald Smith, Allen Taylor
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2015)
Meeting Abstract
Biochemistry & Molecular Biology
Sheldon Rowan, Min-Lee Chang, Shuhong Jiang, Christa Cassalman, Christina McGuire, James Baleja, Barbara Nagel, Steven Fliesler, Donald Smith, Allen Taylor
Article
Ophthalmology
Sheldon Rowan, Karen Weikel, Min-Lee Chang, Barbara A. Nagel, Jeffrey S. Thinschmidt, Amanda Carey, Maria B. Grant, Steven J. Fliesler, Donald Smith, Allen Taylor
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2014)