期刊
DEVELOPMENT
卷 137, 期 6, 页码 953-961出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.041939
关键词
Arabidopsis thaliana; DNA damage; Microtubules; Mitosis; radially swollen 4; Root morphology
资金
- Max-Planck Society
- US Department of Energy [DE-FG02 03ER15421]
- U.S. Department of Energy (DOE) [DE-FG02-03ER15421] Funding Source: U.S. Department of Energy (DOE)
The caspase family protease, separase, is required at anaphase onset to cleave the cohesin complex, which joins sister chromatids. However, among eukaryotes, separases have acquired novel functions. Here, we show that Arabidopsis thaliana radially swollen 4 (rsw4), a temperature-sensitive mutant isolated previously on the basis of root swelling, harbors a mutation in At4g22970, the A. thaliana separase. Loss of separase function in rsw4 at the restrictive temperature is indicated by the widespread failure of replicated chromosomes to disjoin. Surprisingly, rsw4 has neither pronounced cell cycle arrest nor anomalous spindle formation, which occur in other eukaryotes upon loss of separase activity. However, rsw4 roots have disorganized cortical microtubules and accumulate the mitosis-specific cyclin, cyclin B1;1, excessive levels of which have been associated with altered microtubules and morphology. Cyclin B1;1 also accumulates in certain backgrounds in response to DNA damage, but we find no evidence for aberrant responses to DNA damage in rsw4. Our characterization of rsw4 leads us to hypothesize that plant separase, in addition to cleaving cohesin, regulates cyclin B1;1, with profound ramifications for morphogenesis.
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