Article
Cell & Tissue Engineering
Kenneth N. Grise, Brenda L. K. Coles, Nelson X. Bautista, Derek van der Kooy
Summary: The adult mammalian retina lacks the ability to regenerate lost cells, but retinal stem cells (RSCs) in a quiescent state in the ciliary epithelium have the potential to generate all retinal cell types. By antagonizing BMP and sFRP2 proteins, researchers were able to activate RSCs and expand the population, suggesting a potential for endogenous retinal cell therapy.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Cindy L. Olmos-Carreno, Maria Figueres-Onate, Gabriel E. Scicolone, Laura Lopez-Mascaraque
Summary: Clonal cell analysis reveals the ontogenic potential of retinal stem/progenitor cells and uncovers neural heterogeneity among different cell types and lineages. Using a chick embryo model, this study tracks retinal cell lineages derived from individual progenitors and shows the presence of multipotent and committed progenitor cells in the early stages of development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Xiaotong Wang, Mengyuan Qu, Zili Li, Yuting Long, Kai Hong, Honggang Li
Summary: The study developed a protocol to induce spermatogonial stem cell-like cells (SSCLCs) from human pluripotent stem cells, revealing that a combination of low concentrations of VPA and VC was most effective in inducing SSCLCs expressing spermatogonial genes. RNA-seq analysis showed upregulated genes in the Wnt signaling pathway during SSCLC induction. Additionally, hiPSC lines from non-obstructive azoospermia (NOA) patients exhibited decreased SSCLC induction efficiency and reduced expression of genes in the Wnt signaling pathway. The study suggests that the inactivation of the Wnt signaling pathway may be associated with SSC depletion in azoospermia testes.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Developmental Biology
Warren A. Campbell, Allen Tangeman, Heithem M. El-Hodiri, Evan C. Hawthorn, Maddie Hathoot, Sydney Blum, Thanh Hoang, Seth Blackshaw, Andy J. Fischer
Summary: A recent study on chick retinas found that fatty acid binding proteins (FABPs) are highly expressed and play a role in regulating the function of glial cells and microglia. Inhibition of FABPs leads to transitions of glial cells to reactive states and suppresses the formation of progenitor cells, while inhibition of fatty acid synthase (FASN) increases microglia proliferation and suppresses progenitor cell formation.
Article
Anatomy & Morphology
Yuanfa He, Jonathan J. J. Carver, Timothy Erickson, Pierre Le Pabic, Yong Zhu
Summary: This study created a new transgenic zebrafish reporter line Tg(adamts9:EGFP) and used confocal imaging to analyze its expression in various tissues and cells. The results showed strong expression of the adamts9:EGFP transgene in multiple tissues and cells of adult and embryonic zebrafish, suggesting its involvement in the development and physiological functions of various tissues in animals.
DEVELOPMENTAL DYNAMICS
(2023)
Article
Cell Biology
Casandra Carrillo, Vagisha Ravi, Sarika Tiwari, Ellen A. Chernoff, Teri L. Belecky-Adams
Summary: The factors necessary for cell differentiation in the retina are not fully understood. This study focuses on the potential role of transforming growth factor beta-activated kinase 1 (TAK1) in the differentiation of cell types in the chick retina. The results indicate that TAK1 plays a unique role in differentiating and proliferating retinal cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Markus Lechner, Thomas Engleitner, Tea Babushku, Marc Schmidt-Supprian, Roland Rad, Lothar J. Strobl, Ursula Zimber-Strobl
Summary: FoB and MZB cells are functionally distinct mature B cell populations in the spleen, which can be re-programmed through Notch2 activation. The study demonstrates plasticity between these cell types and suggests a potential pathway for MZB cell development in vivo, driven by a singular signaling event.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Sarah N. Bradshaw, W. Ted Allison
Summary: The evolution of the vertebrate eye remains unclear, although studies on the eyes of hagfish and lamprey, the most ancient extant vertebrates, suggest similarities in eye development and retinal neurogenesis genes with jawed vertebrates. Further research on retinal neurogenesis in jawless vertebrates could provide insights into the evolutionary origins of the vertebrate eye. The unexpected finding of retinal growth in hagfish despite their degenerate eye phenotype raises questions about the mechanisms involved.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Natalia Sokolova, Lucie Zilova, Joachim Wittbrodt
Summary: The discovery and study of adult stem cells have revolutionized regenerative medicine. Understanding the differences between anamniote and mammalian stem cells is of significant interest.
CELLS & DEVELOPMENT
(2023)
Article
Immunology
Anna-Karin E. Palm, Sandra Kleinau
Summary: Marginal zone (MZ) B cells are a subset of innate-like B cells mainly found in the spleen, participating in quick responses to blood-borne pathogens and secreting natural antibodies. While typically non-pathogenic, they may contribute to breaking immunological tolerance under specific stimuli.
JOURNAL OF AUTOIMMUNITY
(2021)
Article
Ophthalmology
Tafadzwa Young-Zvandasara, David Brunner, Sarah Welch, Joanne L. Sims, Clairton De Souza, William J. Cunningham, Rachael L. Niederer
Summary: The study aimed to investigate the occurrence, activity, features, rate of proliferative vitreoretinopathy (PVR), and outcomes following rhegmatogenous retinal detachment (RRD) in a large tertiary referral uveitis service. The findings revealed a high rate of RRD in uveitis eyes, accompanied by high rates of PVR and redetachment. Anatomical success was high, but visual outcomes remained unpredictable.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Biology
Kirsty Uttley, Andrew S. Papanastasiou, Manuela Lahne, Jennifer M. Brisbane, Ryan B. Macdonald, Wendy A. Bickmore, Shipra Bhatia
Summary: Enhancers play a critical role in precise modulation of gene expression in development, but the functional consequences of sequence variants in enhancers remain challenging to establish. This study focuses on two PAX6 retinal enhancers, HS5 and NRE, and reveals their distinct activities in different cell types using live imaging and single-cell RNA sequencing. The findings demonstrate the potential for uncovering unique cell type-specific activities in apparently similar enhancers when investigated at high resolution in vivo.
LIFE SCIENCE ALLIANCE
(2023)
Article
Medicine, Research & Experimental
Brian T. Gaudette, Carly J. Roman, Trini A. Ochoa, Daniela Gomez Atria, Derek D. Jones, Christian W. Siebel, Ivan Maillard, David Allman
Summary: Little is known about how cells regulate and integrate distinct biosynthetic pathways governing differentiation and cell division. For B lineage cells it is widely accepted that activated cells must complete several rounds of mitosis before yielding antibody-secreting plasma cells. However, we report that marginal zone (MZ) B cells, innate-like naive B cells known to generate plasma cells rapidly in response to blood-borne bacteria, generate functional plasma cells despite cell-cycle arrest. Further, short-term Notch2 blockade in vivo reversed division-independent differentiation potential and decreased transcript abundance for numerous mTORC1-and Myc-regulated genes. Myc loss compromised plasma cell differentiation for MZ B cells, and reciprocally induced ectopic mTORC1 signaling in follicular B cells enabled division-independent differentiation and plasma cell-affiliated gene expression. We conclude that ongoing in situ Notch2/mTORC1 signaling in MZ B cells establishes a unique cellular state that enables rapid division-independent plasma cell differentiation.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Kwangjin Park, Chunmei Li, Sofia Tsiropoulou, Joao Goncalves, Christine Kondratev, Laurence Pelletier, Oliver E. Blacque, Michel R. Leroux
Summary: Cilia play essential roles in cell motility, fluid flow, and signaling, and are divided into subcompartments with variable structures and functions. CDKL-1, a kinase related to the human CDKL family, controls the length of a specific ciliary subdomain and influences cilium-dependent processes. Overall, cilium length is regulated by various pathways and CDKL-1 is crucial for the construction of a specific ciliary compartment and contributes to development and sensory physiology.
Article
Biochemistry & Molecular Biology
Bing-Chun Liu, Fang-Yuan Liu, Xin-Yue Gao, Yang-Lin Chen, Qiao-Qiao Meng, Yong-Li Song, Xi-He Li, Si-Qin Bao
Summary: The study reveals that Wnt signaling plays a crucial role in driving human pluripotent stem cells towards neural stem cells differentiation and maintaining their self-renewal. CLNSCs exhibit spinal cord properties and high proliferative capacity, providing a new cell model for investigating neurogenesis mechanisms and potential applications in regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cardiac & Cardiovascular Systems
Wolfgang Poller, Stefanie Dimmeler, Stephane Heymans, Tanja Zeller, Jan Haas, Mahir Karakas, David-Manuel Leistner, Philipp Jakob, Shinichi Nakagawa, Stefan Blankenberg, Stefan Engelhardt, Thomas Thum, Christian Weber, Benjamin Meder, Roger Hajjar, Ulf Landmesser
EUROPEAN HEART JOURNAL
(2018)
Article
Cell Biology
Xiaona Chen, Liangqiang He, Yu Zhao, Yuying Li, Suyang Zhang, Kun Sun, Karl So, Fengyuan Chen, Liang Zhou, Leina Lu, Lijun Wang, Xihua Zhu, Xichen Bao, Miguel A. Esteban, Shinichi Nakagawa, Kannanganattu V. Prasanth, Zhenguo Wu, Hao Sun, Huating Wang
Article
Biochemistry & Molecular Biology
Tomohiro Yamazaki, Sylvie Souquere, Takeshi Chujo, Simon Kobelke, Yee Seng Chong, Archa H. Fox, Charles S. Bond, Shinichi Nakagawa, Gerard Pierron, Tetsuro Hirose
Article
Multidisciplinary Sciences
Mari Mito, Mitsutaka Kadota, Kaori Tanaka, Yasuhide Furuta, Kuniya Abe, Shintaro Iwasaki, Shinichi Nakagawa
SCIENTIFIC REPORTS
(2018)
Article
Cardiac & Cardiovascular Systems
Martina Gast, Bernhard H. Rauch, Shinichi Nakagawa, Arash Haghikia, Andrzej Jasina, Jan Haas, Neetika Nath, Lars Jensen, Andrea Stroux, Andreas Boehm, Julian Friebel, Ursula Rauch, Carsten Skurk, Stefan Blankenberg, Tanja Zeller, Kannanganattu V. Prasanth, Benjamin Meder, Andreas Kuss, Ulf Landmesser, Wolfgang Poller
CARDIOVASCULAR RESEARCH
(2019)
Article
Multidisciplinary Sciences
Abu Shufian Ishtiaq Ahmed, Kunzhe Dong, Jinhua Liu, Tong Wen, Luyi Yu, Fei Xu, Xiuhua Kang, Islam Osman, Guoqing Hu, Kristopher M. Bunting, Danielle Crethers, Hongyu Gao, Wei Zhang, Yunlong Liu, Ke Wen, Gautam Agarwal, Tetsuro Hirose, Shinichi Nakagawa, Almira Vazdarjanova, Jiliang Zhou
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Taiwa Komatsu, Saori Yokoi, Koichi Fujii, Mari Mito, Yusuke Kimura, Shintaro Iwasaki, Shinichi Nakagawa
Article
Neurosciences
Michael Kunst, Eva Laurell, Nouwar Mokayes, Anna Kramer, Fumi Kubo, Antonio M. Fernandes, Dominique Foerster, Marco Dal Maschio, Herwig Baier
Article
Neurosciences
Anna Kramer, Yunmin Wu, Herwig Baier, Fumi Kubo
Article
Biology
Paride Antinucci, Adna Dumitrescu, Charlotte Deleuze, Holly J. Morley, Kristie Leung, Tom Hagley, Fumi Kubo, Herwig Baier, Isaac H. Bianco, Claire Wyart
Article
Neurosciences
Yunmin Wu, Marco dal Maschio, Fumi Kubo, Herwig Baier
Review
Neurosciences
Koji Matsuda, Fumi Kubo
Summary: Animals utilize optic flow generated by their own movements to estimate and adjust their body posture and gaze direction. Recent studies have identified the pretectum as a primary center for optic flow processing in vertebrates like zebrafish, showing that it responds to complex visual features relevant to behavior. Additionally, optic flow and motor signals are represented in a region-specific manner in the cerebellum.
FRONTIERS IN NEURAL CIRCUITS
(2021)
Article
Biochemical Research Methods
Fabian Svara, Dominique Foerster, Fumi Kubo, Michal Januszewski, Marco dal Maschio, Philipp J. Schubert, Joergen Kornfeld, Adrian A. Wanner, Eva Laurell, Winfried Denk, Herwig Baier
Summary: This article presents a resource on a serial block-face electron microscopy dataset of the larval zebrafish brain, which includes automated segmentation of neurons, detection of synapses, and reconstruction of visual motion processing circuitry. This resource provides a foundation for synaptic-resolution circuit analyses in the zebrafish nervous system by utilizing high-resolution imaging and efficient tracing tools.
Article
Multidisciplinary Sciences
Piu Banerjee, Fumi Kubo, Hirofumi Nakaoka, Rieko Ajima, Takuya Sato, Tatsumi Hirata, Takuji Iwasato
Summary: It has been found that neurons in the whisker-innervating region of the trigeminal ganglion (TG) in neonatal mice generate spontaneous activity. Some neurons showed correlated activity, which mostly occurred between neurons in close proximity. This spontaneous activity was primarily observed in medium-to-large diameter neurons, characteristic of mechanosensory neurons, and could be blocked by chelation of extracellular calcium. Furthermore, this activity diminished in the adult stage.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Takeshi Chujo, Tomohiro Yamazaki, Tetsuya Kawaguchi, Satoshi Kurosaka, Toru Takumi, Shinichi Nakagawa, Tetsuro Hirose