Review
Cell Biology
Yoshiya Ito, Kanako Hosono, Hideki Amano
Summary: The liver has the ability to regenerate in response to acute liver injury, but liver ischemia-reperfusion injury (IRI) remains a major problem in liver surgery. Non-parenchymal cells in the liver play critical roles in liver repair and regeneration, and recent technological advances have provided insights into their heterogeneity. This review discusses the progress in understanding the biology of liver non-parenchymal cells and the functional role of each cell component in response to liver IRI.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xiaoping Wu, Lingling Shu, Zixuan Zhang, Jingjing Li, Jiuyu Zong, Lai Yee Cheong, Dewei Ye, Karen S. L. Lam, Erfei Song, Cunchuan Wang, Aimin Xu, Ruby L. C. Hoo
Summary: Liver fibrosis results in significant changes in the liver microenvironment, which promotes disease progression. The study demonstrates that increased expression of adipocyte fatty acid binding protein (A-FABP) in liver sinusoidal endothelial cells (LSECs) plays a crucial role in promoting liver fibrosis in mice. A-FABP activates signaling pathways that lead to liver fibrosis progression, suggesting that targeting A-FABP may be a potential therapeutic approach against liver fibrosis.
Article
Biotechnology & Applied Microbiology
Ingrid Wilhelmsen, Mikel Amirola Martinez, Justyna Stokowiec, Chencheng Wang, Aleksandra Aizenshtadt, Stefan Krauss
Summary: This study investigates the phenotypic and functional development of pluripotent stem cell-derived hepatic stellate cells (scHSCs) and liver sinusoidal endothelial cells (scLSECs) during extended in vitro culture. It is crucial to consider the choice of pluripotent stem cell line and experimental timeframe in designing in vitro platforms involving scHSCs and scLSECs. Notch inhibition and co-culturing scHSCs and scLSECs have shown potential in enhancing the stability and maturity of these cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Oncology
Nicolo Manicardi, Anabel Fernandez-Iglesias, Laia Abad-Jorda, Felix Royo, Mikel Azkargorta, Marti Ortega-Ribera, David Sanfeliu-Redondo, Ana Martinez-Alcocer, Felix Elortza, Amelia J. Hessheimer, Constantino Fondevila, Juan Jose Lozano, Juan Carlos Garcia-Pagan, Jaime Bosch, Francisco Javier Cubero, Agustin Albillos, Javier Vaquero, Juan M. Falcon-Perez, Jordi Gracia-Sancho
Summary: This study defines the transcriptome and secretome of primary LSECs during the progression of cirrhosis, revealing specific molecular signatures, novel biomarkers and therapeutic targets for patients with advanced chronic liver disease. The analysis of LSECs in pre-clinical models and fresh human cirrhotic tissue showed common modifications in gene expression related to pro-oncogenic processes and extracellular vesicles biogenesis. The study also identified endothelial EVs as potent angiocrine effectors and discovered stage-specific proteomic signatures that could be potential therapeutic targets for treating cirrhotic patients.
Article
Multidisciplinary Sciences
Jing Fang, Qiang Ji, Siqi Gao, Zhun Xiao, Wei Liu, Yonghong Hu, Ying Lv, Gaofeng Chen, Yongping Mu, Hong Cai, Jiamei Chen, Ping Liu
Summary: This study found that the HIF-1 alpha/CXCR4/PDGF-BB/CXCR7 axis promotes the dedifferentiation of LSECs, thereby triggering the activation of HSCs and liver fibrosis.
Article
Biochemistry & Molecular Biology
Elvira Olaso, Aitor Benedicto, Aritz Lopategi, Fernando P. Cossio, Beatriz Arteta
Summary: Fas21, a resveratrol analog, was used to modulate the function of hepatic stellate cells (HSCs) and liver sinusoidal endothelial cells (LSECs) during the angiogenic phase of murine liver metastasis. Treatment with Fas21 resulted in reduced liver tumor foci in both tumor metastasis models. The inhibitory effect of Fas21 on HSCs and LSECs migration and VEGF secretion suggests its potential as a therapeutic agent for liver metastasis.
BIOMOLECULES & THERAPEUTICS
(2022)
Article
Materials Science, Multidisciplinary
Dong Chen, Yutong Jiang, Zehua Sun, Yuelong Huang, Jian Yu, Tao Chen
Summary: By depositing the ITO/Ag/ITO and ITO/Cu/ITO sandwiched structure at room temperature, the average visible transmittance and sheet resistance of the transparent rear contacts for ST-PSCs can be improved, leading to enhanced opto-electrical properties.
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Materials Science, Ceramics
Beomki Jeon, Sungjun Kim
Summary: In this work, TiN/SiOx/ITO devices were used to achieve conductance quantization and multilevel conductance for high-density memory applications. Stepwise conductance with integer and half-integer multiples was observed under pulse and DC voltages. The conductance quantization of this device is predicted to be a combination of the formation and rupture of oxygen vacancies in the SiOx film and the formation of O-2 rich ITO layers due to the oxidation reaction between ITO and SiOx. Furthermore, TiN/SiOx/ITO RRAM devices were found to stably endure each conductance level.
CERAMICS INTERNATIONAL
(2023)
Article
Immunology
Qianqian Guo, Kunimaro Furuta, Shahidul Islam, Nunzia Caporarello, Enis Kostallari, Kobe Dielis, Daniel J. J. Tschumperlin, Petra Hirsova, Samar H. H. Ibrahim
Summary: The study reveals that VCAM1 not only functions as a scaffold for leukocyte adhesion during liver injury, but also modulates LSEC capillarization and liver fibrosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Neurosciences
Dakota R. Kamm, Kyle S. McCommis
Summary: Hepatic stellate cells (HSCs) play critical roles in the normal liver and in response to injury. They are activated during liver injury and produce extracellular matrix in liver fibrosis. In the absence of injury, HSCs are in a quiescent state and store retinoids or vitamin A-containing metabolites. They also enhance the inflammatory response and express growth factors crucial for liver development and regeneration. Recent studies have identified diverse phenotypic alterations and unique subpopulations of HSCs.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Biochemistry & Molecular Biology
Maximilian Schinagl, Tamara Tomin, Juergen Gindlhuber, Sophie Honeder, Raphael Pfleger, Matthias Schittmayer, Michael Trauner, Ruth Birner-Gruenberger
Summary: This study investigated the activation of hepatic stellate cells (HSC) through an increase in growth medium serum concentration, revealing cellular processes involved in HSC transformation. Activated HSC showed increased production of ribosomal proteins and proteins related to migration and cell cycle control, along with a decrease in cholesterol and fatty acid biosynthesis proteins. The findings provide insights into HSC activation characteristics and present a accessible model for studying HSC activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Laura Talamini, Pierre Picchetti, Lorena Maria Ferreira, Giovanni Sitia, Luca Russo, Martina B. Violatto, Leana Travaglini, Jennifer Fernandez Alarcon, Lucrezia Righelli, Paolo Bigini, Luisa De Cola
Summary: This study evaluates the biocompatibility of novel organosilica nanoparticles in vivo, finding that they have the potential to be used as nanotools for modulating biological processes, impacting tumor cell extravasation and hepatic immune functions.
Article
Cell Biology
Hiroyuki Ogawa, Kosuke Kaji, Norihisa Nishimura, Hirotetsu Takagi, Koji Ishida, Hiroaki Takaya, Hideto Kawaratani, Kei Moriya, Tadashi Namisaki, Takemi Akahane, Hitoshi Yoshiji
Summary: Lenvatinib shows potential as a novel therapeutic strategy for liver fibrosis by inhibiting hepatic stellate cell proliferation and promoting apoptosis, as well as reducing protein expression levels and intrahepatic neovascularization.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Bai Ruan, Juan-Li Duan, Hao Xu, Kai-Shan Tao, Hua Han, Guo-Rui Dou, Lin Wang
Summary: Liver sinusoidal endothelial cells undergo partial endothelial mesenchymal transition during liver fibrosis, leading to increased extracellular matrix production without activating cell mobility. Targeting this transitional process may be valuable for antifibrotic treatment of liver fibrosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Obstetrics & Gynecology
Jackie R. York, Giuliano Testa, Robert T. Gunby, J. Michael Putman, Gregory J. McKenna, Eric C. Koon, Johanna Bayer, Lilly Zhang, Anthony R. Gregg, Liza Johannesson
Summary: This study described the hospital course and laboratory findings in the first 2 months of life of 12 infants born after uterus transplantation. The infants had a gestational age that reflected the time of delivery and showed no identified malformations or organ dysfunctions. At 2 months of age, all infants achieved developmental and behavioral milestones, but longer follow-up and more participants are needed to confirm these observations.
AMERICAN JOURNAL OF PERINATOLOGY
(2023)
Article
Orthopedics
Gennaro Selvaggi, Kristiina Manner, Augustinas Sakinis, Michael Olausson
Summary: Literature reports successful cases of penile transplantation for cis men, with no attempts for trans men. Modern surgical techniques for penis reconstruction may have limitations, making penis transplantation a potential alternative. The study confirmed the feasibility of using the internal pudendal artery for arterial anastomosis in transplantation procedures based on measurements of diameter and length.
JOURNAL OF PLASTIC SURGERY AND HAND SURGERY
(2022)
Review
Obstetrics & Gynecology
S. Vali, B. P. Jones, S. Saso, M. Fertleman, G. Testa, L. Johanesson, A. Alghrani, J. R. Smith
Summary: Uterine transplantation is rapidly progressing from experimental to clinical, with the first trial in the UK prompting a closer look at the legal implications on fertility treatment and transplantation in England.
BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY
(2022)
Article
Obstetrics & Gynecology
Anji E. Wall, Liza Johannesson, Monica Sok, Ann Marie Warren, Elisa J. Gordon, Giuliano Testa
Summary: Absolute uterine factor infertility has a negative impact on women deform a young age, affecting multiple relationships and challenging female identity. Uterus transplantation helps to reverse this impact, transforming women's life narrative of infertility and enhancing female identity.
BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY
(2022)
Article
Cell & Tissue Engineering
Debashish Banerjee, Nikhil B. Nayakawde, Deepti Antony, Meghshree Deshmukh, Sudip Ghosh, Carina Sihlbom, Evelin Berger, Uzair Ul Haq, Michael Olausson
Summary: Biological scaffolds prepared by decellularization and implanted using different routes were studied to understand the differential immune responses elicited. The use of sensitive protein and genomic tools provided valuable insights into the changes associated with chemical decellularization and postimplantation. This study contributes to the development of appropriate chemical strategies for biological scaffolds and the identification of implantable routes to overcome adverse immune reactions.
TISSUE ENGINEERING PART A
(2022)
Article
Immunology
Michael Olausson, Deepti Antony, Galina Travnikova, Martin Johansson, Nikhil B. Nayakawde, Debashish Banerjee, John Mackay Softeland, Goditha U. Premaratne
Summary: This study demonstrates that kidneys from extended uDCD, without aCPR/EC, can be salvaged using thrombolytic treatment to remove fibrin thrombi while preserving histology. These kidneys can then be transplanted with clinically acceptable early function.
Article
Biochemistry & Molecular Biology
Mihai Oltean, Jasmine Bagge, George Dindelegan, Diarmuid Kenny, Antonio Molinaro, Mats Hellstrom, Ola Nilsson, Carina Sihlbom, Anna Casselbrant, Marcela Davila, Michael Olausson
Summary: This study conducted a proteome-wide analysis in a mouse model of intestinal transplantation and identified proteome changes associated with rejection, potentially serving as biomarkers for intestinal rejection.
Article
Surgery
Deirdre Sawinski, Liza Johannesson, Jakub Kristek, Jiri Fronek, Kathleen E. O'Neill, Anthony Gregg, Giuliano Testa, Paige M. Porrett
Summary: Uterus transplantation is an effective treatment for uterine factor infertility, but it carries the risk of immunosuppression and congenital renal anomalies. A study on UTx recipients found a decline in renal function starting at 30 days posttransplant and a high incidence of acute kidney injury and pre-eclampsia during pregnancy. Further research is needed to assess long-term renal risks for UTx recipients.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Obstetrics & Gynecology
Philipp Schulz, Giuliano Testa, Jackie R. York, Liza Johannesson
Summary: This study describes the outcomes for children born in the Dallas Uterus Transplant Study during their first 2 years of life. The results show that the children's growth and physical, neurological, and cognitive development were age appropriate within this period.
BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY
(2022)
Article
Obstetrics & Gynecology
Mats Brannstrom, Pernilla Dahm-Kahler, Niclas Kvarnstrom, Anders Enskog, Jan I. Olofsson, Michael Olausson, Johan Molne, Randa Akouri, Stina Jarvholm, Lars Nilsson, Lennart Stigson, Henrik Hagberg, Hans Bokstrom
Summary: The first completed study of uterus transplantation (UTx) demonstrates that it is an effective treatment for infertility, resulting in the birth of healthy children and having only minor psychological and medical long-term effects on donors and recipients.
FERTILITY AND STERILITY
(2022)
Article
Medicine, General & Internal
Jakub Kristek, Liza Johannesson, Matthew Paul Clemons, Dana Kautznerova, Jaroslav Chlupac, Jiri Fronek, Giuliano Testa, Gregory dePrisco
Summary: Uterus transplantation is a treatment option for women with absolute uterine factor infertility. This article introduces a novel radiologic grading scale for evaluating uterine arteries and veins for transplantation suitability. Magnetic resonance angiography can provide reliable information on uterine venous characteristics when performed consistently and assessed by a dedicated radiologist.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Surgery
Anji E. Wall, Liza Johannesson, Vikrant Reddy, Ann Marie Warren, Elisa J. Gordon, Giuliano Testa
Summary: This study explored the perceptions of living uterus donors regarding informed consent and decision-making processes. The findings indicate that the decision to donate a uterus was influenced by motivations, rationale, and considerations of helping other infertile women. The donors were satisfied with the informed consent process, their experiences were aligned with expectations, and they found value in the act of donation itself. Therefore, living donor uterus programs should develop comprehensive informed consent processes that provide detailed information and encourage shared decision-making with potential donors.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Medicine, General & Internal
Liza Johannesson, Sophie Anderson, J. Michael Putman, Robert T. Gunby, Lilly Zhang, Giuliano Testa, Anthony R. Gregg
Summary: Recipients of uterus transplantation may have an increased risk of cervical insufficiency, and using different cerclage methods may improve this condition. Prophylactic cerclage placement may play an important role at the time of transplantation.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Obstetrics & Gynecology
Liza Johannesson, Giuliano Testa, Nicole Petrillo, Anthony R. Gregg
Summary: Cervical length showed little association with preterm delivery in UTx recipients, but cervical inflammation may be a cause of preterm delivery.
HUMAN REPRODUCTION
(2023)
Meeting Abstract
Surgery
Michael Olausson, Galina Travnikova, Goditha Premaratne, Debashish Banerjee, Nikhil Nayakawde, Martin Johansson, John Softeland, Deepti Antony
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)